• Journal of Mushroom
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• v.13 no.2
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• pp.129-134
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• 2015

Hybrid strains was selected by crossing between dikaryotic strain ASI 0628 (Dagul) bred from ASI 2596 (Suhan No.3) and ASI 2782(Black pileus mutant) and monokaryotic strain ASI 2344-84. The strain 84 that shown the best cultural characteristics was selected to be a new cytoplasmic hybrid variety and named as 'Chunhwashim'. The 'Chunhwashim' shown incompatibility among them forming the confrontation growth line against parental strains ASI 0628 (Dagul) and ASI 2344 (chunchu 2ho). Fruiting body produced about $161.4{\pm}4.8g$ per bottle. And also the individual generation of fruit body is multiple than chunchu2ho as 40.9. The 'Chunhwashim' was cytoplasmic hybrid included hybrid DNA bands of parental strains ASI 0628 (Dagul) and ASI 2344 (chunchu 2ho) by Random Amplified Polymorphic DNA (RAPD) primer, and mitochondria DNA band of monokaryotic ASI 2344 (chunchu 2ho) using mitochondria microsatellite DNA marker. This new cytoplasmic hybrid variety 'Chunhwashim' of oyster mushroom is characterized by multiple of individual generation and deeply grey color of pileus.

• Horticultural Science & Technology
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• v.28 no.4
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• pp.664-671
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• 2010

A genetic linkage map was constructed using 188 $F_9$ RILs derived from a cross between $Solanum$ $lycopersicum$ H7996 (resistant to bacterial wilt) and $S.$ $pimpinellifolium$ WVa700 (highly susceptible to bacterial wilt). The map consisted of 361 markers including 260 DArTs, 74 AFLPs, 4 RFLPs, 1 SNP, and 22 SSRs. The resulting linkage map was comprised of 13 linkage groups covering 2042.7 cM. The genetic linkage map had an average map distance between markers of 5.7 cM, with an average DArT marker density of 1/7.9 cM. Based on the distribution of anchor SSR markers, 11 linkage groups were assigned to 10 chromosomes of tomato except chromosomes 5 and 12. The DArT markers were distributed across the genome in a similar way as other markers and showed the highest frequency of clustering (38.8%) at ${\leq}$ 0.5 cM intervals between adjacent markers, which is 3 times higher than AFLPs (13.5%). The present study is the first utilization of DArT markers in tomato linkage map construction.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.50 no.3
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• pp.205-211
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• 2005

This study was carried out to construct cold-tolerance characteristics. The RILs were developed from progenies of a cross between cold-susceptible Tongil-type rice variety, Milyang 23 and cold-tolerant Japonica rice variety, Stejaree 45 by single seed descent methods. The 175 RILs $(F_8)$ were evaluated for cold tolerance traits by field screening under cold-water irrigation. Frequency distribution of RILs in leaf discoloration, heading delay, culm length reduction and number of spikelets reduction displayed nearly normal distributions with transgressive segregations to either side of parents, while the spikelet fertility reduction and panicle exsertion at low-temperature showed the more or less skewed continuous distribution toward the susceptible parent. Higher heritabilities over $60\%$ were observed in leaf discoloration, spikelet fertility reduction, panicle exsertion, while relatively lower heritabilities less than $40\%$ were observed in culm length reduction, number of spikelets reduction and grain yield reduction. Some cold-tolerance RILs were selected effectively by cold water irrigation, which are expected to be good materials in breeding program for cold tolerance.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.6
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• pp.489-494
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• 1993

The amount of maize being imported from other countries to meet the national demand are increasing every year. Regardless of the usage whether it is for silage or for human consumption, amount of seeds for farmers are ever being increased. In order to solve the problems arising from the seed import, a program for developing waxy hybrids with high quality was set up at the College of Agr., Chungnam National University. The main breeding targets for high quality waxy hybrids are focused on the pericarp thickness. In order to obtain basic information needed for developing hybrids with thin pericarp, six inbred lines all derived from open pollinated Korean waxy lines were diallel crossed. Results obtained indicate that waxy hybrids with thin pericarp can be developed by choosing proper parental lines. Of the six inbreds, Jewon inbred had utmost thin pericarp compared with other lines. Hybrids crossed with Jewon showed also thinner pericarp than other hybrids. However, Danyang which has thick pericarp showed thicker pericarp in hybrid combinations. Variance due to general combining abilities was greater than the variance due to the specific combining abilities, indicating that additive gene effects are more important. The pericarp thickness of waxy hybrid endosperm varied with the parts of pericarp. The germinal side of the pericarp is comparatively thinner than the abgerminal side. The upper part (crown) has thicker pericaip than lower part (tip) of the kernel.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.37 no.5
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• pp.405-412
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• 1992

Growth characters of dry seeded paddy rice was studied at different levels of nitrogen(N) split application (basal : immediately after irrigation : 2 weeks after irrigation: 25 days before heading: heading stage with 10 : 20 : 30 : 20 : 20%, 20 : 30 : 20 : 20 : 10%, 30 : 40 : 0 : 20 : 10%, and 100% basal of slow release urea) in tillage and no-tillage systems. On May 17 in 1990, 6kg /10a of dry seeds of a breeding line of Milyang 95 was broadcasted and covered with a power tiller. Between tillage and no-tillage systems yield and its components, lodging related characteristics, and contents of cellulose, hemicellulose and lignin of culm base were similar. Leaf area index at heading stage was higher in tillage, but lodging index was lower compared with those in no-tillage plot. Culm length, effective tiller ratio, culm base weight, 1000-grain weight, harvest index in slow release N applied plot were higher compared with those in other N treatments. Grain yield of rice among levels of N split applications was similar in tillage, but it was higher in slow release N applied plot in no-tillage if 1kg /10a of additional N was applied as urea at panicle formation stage. At early growth stage N concentration and N uptake were lower in the slow release N applied plot, but higher after heading stage compared with other N treatments. The number of tillers was lowest in slow release N applied plot during the tillering stage, but the number of panicles per hill was similar to other N treatments.

• Current Research on Agriculture and Life Sciences
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• v.12
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• pp.29-34
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• 1994

A leading sweet pepper cultivar, Keystone Resistant Giant #3, was crossed with a line with resistance to Phytophthora capsici, PI201232, for incorporation of the resistance and to study the inheritance of resistance to the disease. Seedlings of parents, $F_1$, $F_2$ and backcross populations of a cross between Keystone Resistant Giant #3 and PI201232 were inoculated with zoospore suspension of P. capsici at 36 days after seeding. Most of the $F_1$ seedlings survied the inoculation and this suggested that resistance is dominant over susceptibility. The number of survived plants in $F_2$ population was, however, much less than the killed. All the plants in a backcross to Keystone Resistant Giant #3 were killed. Therefore, the observed numbers did not fit the expected ratio for segregation of one or two dominant alleles as previously reported. The resistance to P. capsici appeared to be inherited in a quantitative mode in evaluation of root rot. Resistant individuals in $F_2$ population were selected and a breeding program for incorporation of the resistance to P. capsici by backcross method is continued.

• Current Research on Agriculture and Life Sciences
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• v.12
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• pp.23-28
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• 1994

A leading sweet pepper cultivar, Keystone Resistant Giant #3, was crossed with a line with resistance to Xanthomonas campestris pv. vesicatoria, PI271322, for incorporation of the resistance and to study the inheritance of resistance to the disease. Seedlings of parents, $F_1$, $F_2$ and backcross populations of a cross between Keystone Resistant Giant #3 and PI271322 were inoculated with race 1 and race 3 of Xanthamonas campestris pv. vesicatoria by infiltrating bacterial suspension into abaxial side of leaves. PI271322 was carrying a gene ($Bs_3$) for hypersensitive resistance to race 1 of X. c. pv. vesicatoria and also a component of non-hypersensitive resistance to race 3 inheriting in a quantitative mode. Correlation coefficient between disease indices to race 1 and race 3 of non-hypersensitive plants in $F_2$ population was highly significant, thus indicating that a component of non-hypersensitive general resistance in PI271322 acted consistently to both races. Individual plants with resistance to both race 1 and race 3 of X. c. pv. vesicatoria were selected and a breeding program for incorporation of the resistance is continued.

• Journal of Sericultural and Entomological Science
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• v.38 no.2
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• pp.93-99
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• 1996

By treatment(dropping) of 0.1~0.4% colchicine solution on the sprouts of winter buds of hard wood cutting slips for 4~5 days, two lines of artificial autotetraploids from Yongchonppong and one line from Yeongbyonppong were bred and the important cultivative characteristics of those new lines were as follows. The greentip sprouting stage of the new bred lines in spring season is later than the parental varieties by two days, but growth speed of the new lines after sprouting was faster than that of the parental varieties reaching the same level development with the parental varieties at the fifth leaf sprouting stage to be mid varieties same as the origins. The leaf shape of the new bred lines was wide round and the petioloes were long and thick. The thickness of leaf was thicker than the parental varieties by 17-33% and single leaf weight was heavy. The leaf area weight increased by 21-31% and the content of chlorophyll was also higher by 11-33%. With all the characteristics, the new breds produced good quality of leaves. The length and number of branches were shorter and less, respectively, than the parental varieties, but the internode length was either same or longer than the parents. Looking at the characteristics, the constitution of shoots was slightly inferior to the parental varieties. The cold hardness expressed by the death top rate of Sawonppong 23 and Sawonppong 24 was same level as that of Yongchonppong, but Sawonppong 25 was stronger than Yeongbyonppong in it with a high infection rate of dwarf disease. The productivity was lower than the parental varieties, but young shoot rate to shoot and branch and the ratio of leaf to young shoot were higher than the parental varieties. The fertility of Sawonppong 23 and Sawonppong 24 was comparatively high with 62% of cross success, but that of Sawonppong 25 was low with 23.9% of cross success.

• Korean Journal of Environment and Ecology
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• v.8 no.2
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• pp.183-192
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• 1995

This study was conducted to investigate bird community and to suggest a proper way how to manage and protect wildbirds in Chuwangsan National Park. The survey was carried out over two survey sections by the line transect method in spring, summer and autumn in 1994. Total number of observed birds were 42 species(33 species in spring, 32 species in summer, and 26 species in autumn), and the result was the same with the typical pattern of temperate bird communities. Total number of natural monument and rare birds were 7 species, which included Golden Eagle(Aquila chrysaetos), Chinese Sparrow Hawk(Accipter soloensis), Eagle Owl(Bubo bubo), etc. In breeding season, species richness was high in canopy nesting guild, and density(ea/ha) was high in hole nesting guild. Canopy foraging guild was dominated in spring and summer, ratio of bush(or ground) foraging guild was increased in autumn. Since the habitats of observed natural monuments and rare species were roughly overlapped with the nature preservation area, it is necessary to preserve the area completely and to investigate and protect these species continuously. For the protection of the bush(or ground) foraging guild, it needs to plant bushes around trails and to control users. Food plants, artificial food planting of bushs, and artificial nest are needed for the habituation of wildbirds, and as the damage of wild cats is increasing lately, it needs to control these cats.

• Korean Journal of Agricultural Science
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• v.25 no.1
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• pp.6-11
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• 1998

Bean sprout is one of the traditional vegetable in Korea and produced by sprouting soybean (Glycine max L.) cultivars. This experiments was conducted to investigate the ecological characteristics in collected small grain soybean lines from Chungnam province region. Thirty varieties were used in this study. Seed coat color of collected small soybean lines showed eight different types. Major seed coat color type was yellow as 8 lines of total 35 lines. The stem height, number of nodes and number of branches averaged 58.5 cm, 16.5 nodes and 11.8 branches, respectively. The average number of main stem pods and number of branching pod were appeared 43.1 and 62.7 respectively. One hundred seed weight averaged 9.9g which was lower than 10~12g of sprouting soybean cultivars as breeding goal of Korea. The average of node width showed 13.2cm and long stem open types and short stem close types were classified 1 line and 14 lines. The flower dates of collecting small soybean lines were between 8 Aug. and 13 Aug. and flowering duration was 74~85 days. The date of maturity were between 12 Sep. and 12 Oct., and maturity duration showed great variation among cultivated soybean lines.