• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 춘계학술대회
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• pp.42-42
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• 2019

This study was carried out to build a database system for amylose and protein contents of rice germplasm based on NIRS (Near-Infrared Reflectance Spectroscopy) analysis data. The average waxy type amylose contents was 8.7% in landrace, variety and weed type, whereas 10.3% in breeding line. In common rice, the average amylose contents was 22.3% for landrace, 22.7% for variety, 23.6% for weed type and 24.2% for breeding line. Waxy type resources comprised of 5% of the total germplasm collections, whereas low, intermediate and high amylose content resources share 5.5%, 20.5% and 69.0% of total germplasm collections, respectively. The average percent of protein contents was 8.2 for landrace, 8.0 for variety, and 7.9 for weed type and breeding line. The average Variability Index Value was 0.62 in waxy rice, 0.80 in common rice, and 0.51 in protein contents. The accession ratio in arbitrary ranges of landrace was 0.45 in amylose contents ranging from 6.4 to 8.7%, and 0.26 in protein ranging from 7.3 to 8.2%. In the variety, it was 0.32 in amylose ranging from 20.1 to 22.7%, and 0.51 in protein ranging from 6.1 to 8.3%. And also, weed type was 0.67 in amylose ranging from 6.6 to 9.7%, and 0.33 in protein ranging from 7.0 to 7.9%, whereas, in breeding line it was 0.47 in amylose ranging from 10.0 to 12.0%, and 0.26 in protein ranging from 7.0 to 7.9%. These results could be helpful to build database programming system for germplasm management.

• Journal of Ginseng Research
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• 제38권2호
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• pp.130-135
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• 2014

Background: Panax ginseng, the most famous medicinal herb, has a highly duplicated genome structure. However, the genome duplication of P. ginseng has not been characterized at the sequence level. Multiple band patterns have been consistently observed during the development of DNA markers using unique sequences in P. ginseng. Methods: We compared the sequences of multiple bands derived from unique expressed sequence tagsimple sequence repeat (EST-SSR) markers to investigate the sequence level genome duplication. Results: Reamplification and sequencing of the individual bands revealed that, for each marker, two bands around the expected size were genuine amplicons derived from two paralogous loci. In each case, one of the two bands was polymorphic, showing different allelic forms among nine ginseng cultivars, whereas the other band was usually monomorphic. Sequences derived from the two loci showed a high similarity, including the same primer-binding site, but each locus could be distinguished based on SSR number variations and additional single nucleotide polymorphisms (SNPs) or InDels. A locus-specific marker designed from the SNP site between the paralogous loci produced a single band that also showed clear polymorphism among ginseng cultivars. Conclusion: Our data imply that the recent genome duplication has resulted in two highly similar paralogous regions in the ginseng genome. The two paralogous sequences could be differentiated by large SSR number variations and one or two additional SNPs or InDels in every 100 bp of genic region, which can serve as a reliable identifier for each locus.

• Asian-Australasian Journal of Animal Sciences
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• 제27권4호
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• pp.471-478
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• 2014

Investigating gene expression of immune cells of whole blood or peripheral blood mononuclear cells (PBMC) under polyinosinic:polycytidylic acid (poly I:C) stimulation is valuable for understanding the immune response of organism to RNA viruses. Quantitative real-time PCR (qRT-PCR) is a standard method for quantification of gene expression studies. However, the reliability of qRT-PCR data critically depends on proper selection of reference genes. In the study, using two different analysis programs, geNorm and NormFinder, we systematically evaluated the gene expression stability of six candidate reference genes (GAPDH, ACTB, B2M, RPL4, TBP, and PPIA) in samples of whole blood and PBMC with or without poly I:C stimulation. Generally, the six candidate genes performed a similar trend of expression stability in the samples of whole blood and PBMC, but more stably expressed in whole blood than in PBMC. geNorm ranked B2M and PPIA as the best combination for gene expression normalization, while according to NormFinder, TBP was ranked as the most stable reference gene, followed by B2M and PPIA. Comprehensively considering the results from the two programs, we recommended using the geometric mean of the three genes, TBP, PPIA and B2M, to normalize the gene expression of whole blood and PBMC with poly I:C stimulation. Our study is the first detailed survey of the gene expression stability in whole blood and PBMC with or without poly I:C stimulation and should be helpful for investigating the molecular mechanism involved in porcine whole blood and PBMC in response to poly I:C stimulation.

• Asian-Australasian Journal of Animal Sciences
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• 제21권10호
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• pp.1411-1415
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• 2008

The purpose of the present study was to determine the onset of breeding season, the occurrence of silent and true heats and the duration of estrus in female Markhoz goats of the Kurdistan province in Iran. Ten, 3 years-old Markhoz does with an average weight of $34.05{\pm}2.62kg$ and with one kidding record, were used. The goats were maintained in an open barn under constant nutritional levels and natural photoperiod. One aproned buck was used twice a day every 12 h to detect estrus from mid August to early January. For the determination of the onset of reproductive activity as well as occurrence of silent heat, blood samples were collected every 10 days, from the beginning of the experiment. After $2^{nd}$ standing heat, blood samples were obtained twice a week in order to assess luteal activity and the length of estrous cycles. In this study, estrous behavior was observed including sniffing, vocal exchange, following courtship, flehman, standing heat and mounting. The results of the progesterone assay indicated that in this goat silent heats occur mostly in the early breeding season. The first standing heat was observed in mid-October which was considered as the onset of the breeding season. Duration of the estrous cycle and estrus was recorded as being $20.93{\pm}1.56days$ and $38.86{\pm}15.19h$, respectively. The correlation coefficient between length of daylight and occurrence of estrus was negative (r = -0.470) but not significant (p>0.05). The data showed that there was no significant effect of body weight on estrous cycles (first, second and third) and estrous periods (first, second, third and fourth). Progesterone levels were not significantly different in the first, second and third estrous cycles at days 0, 4, 10, 12, 14 and 19. The results of progesterone assay during the estrous cycle indicate that follicular and luteal phases last 4-5 and 14-15 days, respectively and the concentration of serum progesterone in these phases was $0.88{\pm}0.08$ and $7.44{\pm}0.26ng/ml$, respectively. The study concluded that Markhoz does could be considered as a breed with a short breeding season and an optimal estrous activity in autumn.

• Journal of Microbiology and Biotechnology
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• 제22권9호
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• pp.1218-1223
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• 2012

${\varepsilon}$-Poly-$_L$-lysine (${\varepsilon}$-PL), produced by Streptomyces or Kitasatospora strains, is a homo-poly-amino acid of $_L$-lysine, which is used as a safe food preservative. The present study investigates the combined use of cell immobilization and in situ adsorption (ISA) to produce ${\varepsilon}$-PL in shaken flasks. Loofah sponge-immobilized Streptomyces ahygroscopicus GIM8 produced slightly more ${\varepsilon}$-PL than those immobilized on synthetic sponge, and sugarcane bagasse. Moreover, loofah sponge supported the maximum biomass. Hence, loofah sponge was chosen for cell immobilization. Meanwhile, the ion-exchange resin D152 was employed for ISA. The loofah sponge-immobilized cells produced $0.54{\pm}0.1g/l$ ${\varepsilon}$-PL, which significantly increased to $3.64{\pm}0.32g/l$ after combining with ISA through the addition of resin bags. The free cells with ISA using the dispersed resin yielded $2.73{\pm}0.26g/l$ of ${\varepsilon}$-PL, an increase from $0.82{\pm}0.08g/l$. These data illustrate that the proposed combination method improved production most significantly compared with either immobilization or ISA only. Moreover, the immobilized cells could be repeatedly used and an ${\varepsilon}$-PL total amount of $8.05{\pm}0.84g/l$ was obtained. The proposed combination method offers promising perspectives for ${\varepsilon}$-PL production.

• Asian-Australasian Journal of Animal Sciences
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• 제33권11호
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• pp.1741-1754
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• 2020

Objective: This study was conducted to estimate genetic parameters for milk yield traits using daily milk yield records from parlour data generated in an intensively managed commercial dairy farm with Jersey and Jersey-Friesian cows in Sri Lanka. Methods: Genetic parameters were estimated for first and second lactation predicted and realized 305-day milk yield using univariate animal models. Genetic parameters were also estimated for total milk yield for each 30-day intervals of the first lactation using univariate animal models and for daily milk yield using random regression models fitting second-order Legendre polynomials and assuming heterogeneous residual variances. Breeding values for predicted 305-day milk yield were estimated using an animal model. Results: For the first lactation, the heritability of predicted 305-day milk yield in Jersey cows (0.08±0.03) was higher than that of Jersey-Friesian cows (0.02±0.01). The second lactation heritability estimates were similar to that of first lactation. The repeatability of the daily milk records was 0.28±0.01 and the heritability ranged from 0.002±0.05 to 0.19±0.02 depending on day of milk. Pearson product-moment correlations between the bull estimated breeding values (EBVs) in Australia and bull EBVs in Sri Lanka for 305-day milk yield were 0.39 in Jersey cows and -0.35 in Jersey-Friesian cows. Conclusion: The heritabilities estimated for milk yield in Jersey and Jersey-Friesian cows in Sri Lanka were low, and were associated with low additive genetic variances for the traits. Sire differences in Australia were not expressed in the tropical low-country of Sri Lanka. Therefore, genetic progress achieved by importing genetic material from Australia can be expected to be slow. This emphasizes the need for a within-country evaluation of bulls to produce locally adapted dairy cows.

• Journal of Periodontal and Implant Science
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• 제46권1호
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• pp.22-34
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• 2016

Purpose: Gingival recession is a major esthetic concern and may lead to root sensitivity during periodontal treatment. Coronally advanced flaps (CAFs) with and without acellular dermal matrix (ADM) are widely used in root coverage procedures. The aim of this study was to analyze the efficacy of CAF in combination with ADM in the treatment of gingival recession. Methods: PubMed, The Cochrane Library, and Embase were used to identify relevant articles. The articles were screened, data were extracted, and the quality of the studies was assessed by three reviewers with expertise in clinical practice, trials, statistics, and biomedical editing. The clinical endpoints of interest included changes in recession, probing depth (PD), clinical attachment level (CAL), and keratinized tissue (KT). Results: Ten randomized controlled trials were identified, including six studies that compared CAFs with ADM and CAFs using connective tissue grafting (CTG) and four studies that compared CAFs with or without ADM. No statistically significant differences were found between the use of ADM and CTG, whereas statistically significant differences were found between groups in which ADM and CAF were combined and groups that underwent CAF alone with regard to recession coverage, CAL, and KT. The combination of CAF with an ADM allograft achieved more favorable recession coverage and recovery of CAL and KT than CAF alone. Conclusions: The results from the ADM and CTG groups suggest that both procedures may be equally effective in clinical practice. Given the limitations of this study, further investigation is needed to clarify the effectiveness of ADM and CAF in clinical practice.

• 한국수정란이식학회지
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• 제32권3호
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• pp.171-176
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• 2017

본 연구는 한우 송아지 생산효율 향상을 위한 농가 번식 실태를 조사하였다. 한우 사육 규모별, 지역별로 나누어 45개 농가의 가임암소를 대상으로 조사를 실시하여 다음과 같은 결과를 얻었다. 한우의 최초 분만월령은 평균 28.7개월령이었다. 수태당 인공수정 횟수는 $1.45{\pm}0.32$회였으며, 분만 후 인공수정 일수는 119.8일이었다. 수태율은 소규모 농가가 $75.2{\pm}16.93%$로 중규모($70.6{\pm}17.46%$) 및 대규모 농가($71.4{\pm}11.03%$)보다 높은 것으로 나타났다. 번식률 상위 농가의 사육 방식을 보면, '발정관찰 보조기구를 사용하는 농가'가 '그렇지 않은 농가'보다 송아지 생산율이 10.42% 높았다. 번식소에 대해 IBR과 BVDV 예방접종을 실시한 농가는 유사산 폐사율이 4.41% 감소한 것으로 나타났다. 또한 농가여건에 따라 방목과 운동을 한 경우, 수태율과 분만율이 각각 3.47%, 18.29% 향상되는 것으로 나타났다. 본 조사 결과를 통해 발정관찰, 예방접종, 방목이 한우농가에서 송아지 생산 효율을 높이는데 중요한 지표로 나타났다. 한우 사육 농가의 번식실태 조사를 통해 농가 현장의 번식률 향상을 위한 연구 자료로 활용이 가능할 것이다.

• 생명과학회지
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• 제32권4호
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• pp.279-284
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• 2022

본 연구는 일반농가에서 적용 가능한 유전평가시스템을 구축을 위해 경기 지역에서 사육중인 암소 619두를 BLUP (Best Linear Unbiased Prediction)과 GBLUP (Genomic Best Linear Unbiased Prediction)을 사용하여 각 형질(도체중, 등심단면적, 등지방두께, 근내지방도) 별 추정 육종가의 정확도를 비교분석 하였다. GBLUP의 경우 참조집단의 크기를 다르게 그룹을 나누어 분석하였다. 분석결과 GBLUP 참조집단의 크기가 커질수록 각 형질의 육종가의 정확도도 상승하는 것을 확인 하였다. BLUP과 GBLUP 방법을 사용하여 추정한 육종가의 정확도를 비교하면, GBLUP 방법을 사용하여 육종가를 추정하였을 때 도체중, 등심단면적, 등지방두께 근내지방도순으로 각각 0.10, 0.09, 0.09, 0.11 이상 상승한 것을 확인할 수 있었다. 따라서, GBLUP 방법을 암소 평가 및 선발에 적용한다면, 정밀하고 정확한 개체 선발이 가능하고 참조집단의 크기를 더욱 키운다면 보다 정확한 개체 선발을 할 수 있기 때문에 선발의 효율성이 증가할 것으로 사료된다.

• Journal of the Korean Data and Information Science Society
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• 제22권3호
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• pp.515-521
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• 2011

'말산업육성법' 제정에 따라 국내 승마산업의 저변확대를 위해 자질이 우수한 국내산 승용마 생산 및 개량에 대한 연구가 절실히 필요한 실정이다. 따라서 본 논문에서는 국내에서 승용마로 활용되고 있는 3품종 (웜블러드, 더러브렛, 제주산마) 32두에 대해 12항목의 체형을 측정하여, 측정자료를 바탕으로 판별분석을 실시한 결과 81.3%가 정확하게 분류되는 것을 확인 할 수 있었다. 본 연구의 결과는 향후 체구성 분석을 통한 말의 유형 (경주마, 승용마, 재활치료마, 역용마, 비육마) 및 승용마 외모 심사를 판단하는 모형 개발에 기초자료로 활용될 것이며 이는 향후 3D 영상촬영측정치를 활용한 한국형 승용마 생산 및 개량화 연구에 활용될 것으로 사료된다.