• The Korean Journal of Nuclear Medicine
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• v.39 no.1
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• pp.21-25
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• 2005

Purpose: $^{14}C$-urea breath test (UBT) is a non-invasive and reliable method for the diagnosis of Helicobacter pylori (HP) infection. In this study, we evaluated the diagnostic performance of a new and rapid $^{14}C$-UBT (Heliprobe method), which was equipped with $Geiger-M\ddot{u}ller$ counter and compared the results with those obtained by using the conventional method. Materials and Methods: Forty-nine patients with dyspepsia underwent gastroduodenoscopy and $^{14}C$-UBT. A 37 KBq $^{14}C$-urea capsule was administered to patients and breath samples were collected. In Heliprobe method, patients exhaled into a Hellprobe BreathCard for 10 min. And then the activities of the BreathCard were countered using Heliprobe analyzer. In the conventional method, results were countered using liquid scintillation counter. During gastroduodenoscopy, 18 of 49 patients were underwent biopsies. According to these histologic results, we evaluated the diagnostic performance of two different methods and compared them. Also we evaluated the concordant and disconcordant rates between them. Results: In all 49 patients, concordant rate of both conventional and Heliprobe methods was 98% (48/49) and the discordant rate was 2% (1/49). Thirteen of 18 patients to whom biopsies were applied, were found to be HP positive on histologic results. And both Heliprobe method and conventional method classified 13 of 13 HP-positive patients and 5 of 5 HP-negative patients correctly (sensitivity 100%, specificity 100%, accuracy 100%). Conclusion: The Heliprobe method demonstrated the same diagnostic performance compared with the conventional method and was a simpler and more rapid technique.

• Journal of Pharmaceutical Investigation
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• v.32 no.1
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• pp.7-11
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• 2002

The purpose of this study was to develop a new $^{l3}C-urea-containing$ capsule for diagnosis of H. pylori. The urea-containing capsules were prepared with various diluents such as polyethylene glycol (PEG), microcrystalline cellulose, sodium lauryl sulfate and citric acid. The dissolution test, $^{l3}C-urea$ breath test and stability test were then performed on the capsules. Microcrystalline cellulose and sodium lauryl sulfate retarded the initial dissolution rates of urea. However, PEG increased the initial dissolution rates of urea. Furthermore, two formulae composed of PEG, [$^{l3}C-urea/PEG$ (38/1.9 mg/cap)] and [$^{l3}C-urea/PEG/citric$ acid (38/1.9/1.9 mg/cap)] had the maximum DOB value, about 16 at 20 mim, while the formula composed of only 38 mg $^{l3}C-urea$ had the maximum DOB value at 30 min. The results indicated that PEG improved the, sensitivity of $^{l3}C-urea$ in the human volunteers. The capsule [$^{l3}C-urea/PEG$ (38/1.9 mg/cap)] was stable for at least six months in 25 and $37^{\circ}C$. Thus, a PEG-containing capsule, [$^{l3}C-urea/PEG$ (38/1.9 mg/cap)] would be a more economical, sensitive and stable preparation for diagnosis of H. pylori.

• Journal of Korean society of Dental Hygiene
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• v.11 no.6
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• pp.843-852
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• 2011

Objectives : The aims of the study were to examine perceived oral malodor and self-reported need for oral and dental care among patients receiving oral prophylaxis services and provide guidelines for developing educational programs(toothbrushing method and tongue cleaning) for oral hygiene and oral malodor prevention. Methods : The survey was administered to a sample of 462 receiving voluntary oral prophylaxis service in a dental laboratory at the J School of Public Health in Korea. The subjects were asked a range of questions related to the degrees of perceived oral malodor and concern for oral health status, as well as their demographic information and need for oral and dental treatment. Univariate analyses using Chi-square and T-test with a P-value of .05 were performed using SPSS Version 12.0 for Windows. Results : 1. Male participants reported "moderately concerned for bad breath and smell" and "I don't care bad breath and smell" 39.1% and 26.2% respectively, while more female participants were concerned for oral malodor. "moderately concerned for malodor" and" highly concerned for malodor" 41.1% and 28.5%(p<.05). a significant difference among age groups was found. 19% of young adults (less than or equal to 29 years of age) reported "highly concerned for bad breath and smell" while 36.4% of older adults (greater than or equal to 50 years of age) reported "highly concerned for bad breath and smell"(p<.05). 2. 12 non-smoking participants (3.7%) and 15 smoking participants (10.8%) reported that they have perceived bad breadth and smell (p<.05). 3. Smoking participants reported a higher degree of need for oral malodor treatment than that of non-smoking participants 88.5% and 82% respectively(p<.05). 4. The participants who did regular toothbrushing more than 3 times a day reported "no malodor", 77 % as compared to 66.7% of the participants who did regular toothbrushing 2 times a day. Toothbrushing 2 times a day reported either "moderate malodor" or "sever malodor"(p<.01). Participants with more frequent toothbrushing reported less oral and breath odor as compared participants with less frequent toothbrushing. Conclusions : The study suggested that there is a need to oral prophylaxis for prevention and toothbrushing and tongue cleaning method oral malodor care and oral health status.

• Journal of dental hygiene science
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• v.20 no.3
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• pp.126-135
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• 2020

Background: The purpose of this study is to investigate the effects of entrance exam stress on oral health behaviors and subjective oral health status in female high school students. Methods: A self-reported questionnaire was administered to 216 female high school students in the Gwangju area. We performed an independent t-test, one-way analysis of variance (ANOVA), Pearson's correlation coefficient, and multiple regression analysis using the SPSS/WIN 21.0 program. Results: The average of the entrance exam stress was 2.73, and among the sub-areas, the tension for exam/poor stress was the highest with 3.21. As a result of analysis of general characteristics and subjective oral health status, oral mucosal disease was lower in family income level (p<0.05), bad breath was significantly higher with lower academic performance (p<0.001) and family income level (p<0.05). As a result of oral health behavior and subjective oral health status analysis, dental caries has more than 4 times of caries snacks (per week) (p<0.05), bad breath was found to be more perceived when the average number of daily brushing was 2 or less (p<0.01). Factors affecting subjective oral health status were that dental caries was a patients pressure stress (β=0.202); temporomandibular disorder (β=0.227), xerostomia (β=0.342), and oral mucosal disease (β=0.190) were insufficient spare time; bad breath was academic performance (lower) (β=0.231) and insufficient spare time (β=0.184). There was a statistically significant positive correlation between the subjective oral health status and the subarea of entrance exam stress, excluding future uncertainty stress. Conclusion: It is considered that oral health education should be conducted to prevent oral diseases for students with high parental pressure and insufficient spare time stress as well as finding practical ways to reduce entrance stress.

• Toxicological Research
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• v.26 no.4
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• pp.329-337
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• 2010

Inhaled inorganic dusts, such as coal, can cause inflammation and fibrosis in the lungs, known as pneumoconiosis. Diagnosis of pneumoconiosis depends on morphological changes by radiological findings and functional change by pulmonary function test (PFT). Unfortunately, current diagnostic findings are limited only to lung fibrosis, which is usually irreversibly progressive. Therefore, it is important that research on potential and prospective biomarkers for pneumoconiosis should be conducted prior to initiation of irreversible radiological or functional changes in the lungs. Analytical techniques using exhaled breath condensate (EBC) or exhaled gas are non-invasive methods for detection of various respiratory diseases. The objective of this study is to investigate the relationship between inflammatory biomarkers, such as EBC pH or fractional exhaled nitric oxide ($FE_{NO}$), and pneumoconiosis among 120 retired coal miners (41 controls and 79 pneumoconiosis patients). Levels of EBC pH and FENO did not show a statistically significant difference between the pneumoconiosis patient group and pneumoconiosis patients with small opacity classified by International Labor Organization (ILO) classification. The mean concentration of $FE_{NO}$ in the low percentage $FEV_1$ (< 80%) was lower than that in the high percentage (80% $\leq$) (p = 0.023). The mean concentration of $FE_{NO}$ in current smokers was lower than that in non smokers (never or past smokers) (p = 0.027). Although there was no statistical significance, the levels of $FE_{NO}$ in smokers tended to decrease, compared with non smokers, regardless of pneumoconiosis. In conclusion, there was no significant relationship between the level of EBC pH or $FE_{NO}$ and radiological findings or PFT. The effects between exhaled biomarkers and pneumoconiosis progression, such as decreasing PFT and exacerbation of radiological findings, should be monitored.

• Journal of the Korean Home Economics Association
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• v.41 no.6
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• pp.1-12
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• 2003

The purpose of this study were to analyze the anthropometric data of feet of Korean women with aging, to categorize the women's foot shapes, and to compare the shoe size according to the foot shapes in order to provide the basic information for more comfortable shoes. Subjects of this study were 181 women over age 20. They were measured with the direct measurement method and the indirect measurement method. 26 items were measured from the right foot and 6 items were taken on foot outline. Factor analysis, cluster analysis, analysis of variance, post-hoc test, and cross tabs were peformed for statistical analysis of the data by SPSS program. There were significant differences in height items, breadth items, girth items, and angle items by subjects' age. The older subjects' feet were wide and thick with big deformity on toes. The arch height of the older ones was low. This implicates that the degree of deformity on toes, the foot ratio, the foot girth, the foot breath and the arch height as well as the foot length are needed to be considered in developing comfortable shoes. Nine foot construction factors were extracted by the factor analysis of anthropometric measurements; foot size factor, heel and instep factor, malleolus lateralis factor, malleolus medialis factor, foot shape factor, shape of toes factor, heel height factor, big toe height factor, and internal factor. On the basis of the cluster analysis, three different foot shapes were categorized. Type 1 was large and wide foot with little deformity on little toe. Type 2 was medium foot with deformation of big toe, and with the lowest arch height. Type 3 was small and narrow foot with the highest arch height. Distribution of shoe size according to the foot shape was analyzed. The ball of foot breath was of wide distribution than the ball of foot girth. This implicates that girth items and breath items of the foot should be enclosed for the same foot length in the shoe sizing system.

• The Korean Journal of Nuclear Medicine
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• v.35 no.1
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• pp.61-68
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• 2001

Purpose: The C-14 urea breath test (C-14 UBT) is the most specific noninvasive method to detect Helicobacter (H) pylori infection. We investigated if the C-14 UBT can reflect the presence and degree of H. pylori detected by gastroduodenoscopic biopsies (GBx). Materials and methods: One hundred fifty patients (M:F=83:67, age $48.6{\pm}11.2$ yrs) underwent C-14 UBT, rapid urease test (CLO test) and GBx on the same day. For the C-14 UBT, a single breath sample was collected at 10 minutes after ingestion of C-14 urea (137 KBq) capsule and counting was done in a liquid scintillation counter for 1 minute, and the results were classified as positive (${\geq}200dpm$), Intermediate ($50{\sim}199dpm$) or negative (<50 dpm). The results of CLO tests were classified as positive or negative according to color change. The results of GBx on giemsa stain were graded 0 (normal) to 4 (diffuse) according to the distribution of H. pylori by the Wyatt method. We compared C-14 UBT results with GBx grade as a gold standard. Results: In the assessment of the presence of H. pylori infection, the C-14 UBT global performance yielded sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and accuracy of 92.5%, 88.4%, 97.1%, 88.4% and 91.3%, respectively. However, the CLO test had sensitivity, specificity, PPV, NPV and accuracy of 83.2%, 81.4%, 91.8%, 81.4% and 82.7%, respectively. The quantitative values of the C-14 UBT were $45{\pm}27$ dpm in grade 0, $707{\pm}584dpm$ in grade 1, $1558{\pm}584dpm$ in grade 2, $1851{\pm}604dpm$ in grade 3, and $2719{\pm}892dpm$ in grade 4. A significant correlation (r=0.848, p<0.01) was found between C-14 UBT and the grade of distribution of H. pylori infection on GBx with giemsa stain. Conclusion: We conclude that the C-14 UBT is a highly accurate, simple and noninvasive method for the diagnosis of ongoing H. pylori infection and reflects the degree of bacterial distribution.

• Progress in Medical Physics
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• v.25 no.2
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• pp.72-78
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• 2014

The aim of the study is to test a hypothesis that quasi-breath-hold (QBH) biofeedback improves the residual respiratory motion management in gated 3D thoracic MR imaging, reducing respiratory motion artifacts with insignificant acquisition time alteration. To test the hypothesis five healthy human subjects underwent two gated MR imaging studies based on a T2 weighted SPACE MR pulse sequence using a respiratory navigator of a 3T Siemens MRI: one under free breathing and the other under QBH biofeedback breathing. The QBH biofeedback system utilized the external marker position on the abdomen obtained with an RPM system (Real-time Position Management, Varian) to audio-visually guide a human subject for 2s breath-hold at 90% exhalation position in each respiratory cycle. The improvement in the upper liver breath-hold motion reproducibility within the gating window using the QBH biofeedback system has been assessed for a group of volunteers. We assessed the residual respiratory motion management within the gating window and respiratory motion artifacts in 3D thoracic MRI both with/without QBH biofeedback. In addition, the RMSE (root mean square error) of abdominal displacement has been investigated. The QBH biofeedback reduced the residual upper liver motion within the gating window during MR acquisitions (~6 minutes) compared to that for free breathing, resulting in the reduction of respiratory motion artifacts in lung and liver of gated 3D thoracic MR images. The abdominal motion reduction in the gated window was consistent with the residual motion reduction of the diaphragm with QBH biofeedback. Consequently, average RMSE (root mean square error) of abdominal displacement obtained from the RPM has been also reduced from 2.0 mm of free breathing to 0.7 mm of QBH biofeedback breathing over the entire cycle (67% reduction, p-value=0.02) and from 1.7 mm of free breathing to 0.7 mm of QBH biofeedback breathing in the gated window (58% reduction, p-value=0.14). The average baseline drift obtained using a linear fit was reduced from 5.5 mm/min with free breathing to 0.6 mm/min (89% reduction, p-value=0.017) with QBH biofeedback. The study demonstrated that the QBH biofeedback improved the upper liver breath-hold motion reproducibility during the gated 3D thoracic MR imaging. This system can provide clinically applicable motion management of the internal anatomy for gated medical imaging as well as gated radiotherapy.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.264-265
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• 2000

Helicobacter pylori(H. pylori) is the causative agent of chronic gastritis and the single most important factor in peptic ulcer disease, however, the pathogenetic mechanisms underlying H, pylori infection are not well understood. Futhermore, there is a strong association between H. pylori infection and gastric cancer. Various diagnostic methods for detecting H. pylori infection are available. These can be divided into invasive methods, requiring endoscopy, and non-invasive tests, mainly 13C-urea breath tests and serologic detection of antibodies. Rapid urease test is the most recommendable endoscopic test for the diagnosis of H. pylori infection, presently. CLO test kit is the represent of rapid urease test kits. The principles of CLO test kit is that hydrolysis of urea by urease Is detected by a dye indicators showing a color change. Our device is used same principle but we improved the reaction time is more faster and positive color change is more distinctive from the color of the negative specimen. So, this kit is more reliable because it response faster and accuracy.

• Journal of Haehwa Medicine
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• v.8 no.1
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• pp.827-836
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• 1999

Numerous persons complained dyspepsia even though no specific objective findings are revealed by imaging study, laboratory examinations and other clinical research. To find out of so many symptoms what they are, I would to approach by two ways. One way is oriental medical literatural study and the other is Helicobacter pylori infection that is accepted as one of most important causal factors of many gastric diseases. Background/Aims: Recently, the role of Helicobacter pylori as a causal factor in the etiology of gastric cancer, peptic ulcer, gastritis and low-grade gastric mucosa-associated lymphoid tissue(MALT) lymphoma is well known. Using endoscopy, biopsy urease testing and histology are recommanded as the tests of choice. Serological test is not recommanded at the moment because of its low sensitivity and espicially low specificity. The urea breath test is more sensitive and specific noninvasive test than serologic test, but it is not widely available yet. Methods/Results: We studied 90 cases by diagnostic endoscopy as a screening test for the persons complaining gastrointestinal symptoms. As a result eighteen persons are revealed to be Helicoacter pylori infected histologicaly. Conclusion: More specific literatural studies are requied.