• Journal of Bio-Environment Control
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• v.21 no.3
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• pp.276-280
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• 2012

This study was conducted to determine the ideal beginning date of plastic house optimum heating for off-season (December) production of asparagus. To achieve this, the 1.6-year-old 'Green tower' asparagus was cultivated in Jeju area ($33^{\circ}$28.110N, $126^{\circ}$31.076E) and the yield and quality among 7 different beginning dates of heating from Oct. 25 to Jan. 20 with 15-days interval were compared. The heating was controlled to maintain the inside temperature was higher than $20^{\circ}C$, But the plastic house was ventilated when the inside temperature rose to $30^{\circ}C$. Days to sprouting took longer as heating dates were delayed. Days to sprouting took 52 days in heating at December 30, and the days were shortened thereafter. As the beginning of heating was delayed, sprouting of asparagus was retarded. Among treatments, days to sprouting was 52 days and longest when the heating began on December 30. When the heating began on October 25, harvest was earliest and started on 7 November. When the heating began on November 15 and 30, harvest started on November 30 and December 18, respectively. Harvesting started on November 7 for October 25 heating, November 30 for Nov. 15 heating, and December 18 for November 30 heating, respectively. When the heating began was done after December. 15, harvesting was possible after early January. The number of spear, spear weight and yield were highly increased in the treatments of heating after January. 10 when the asparagus dormancy was broken. In the case of heating dates were before the asparagus dormancy breaking, the total yield was highest in November 15 heating with 607 kg/10a. The marketable yield of November 15 heating was 386kg/10a, which was twice higher than the 193 kg/10a of October 25 heating. Accordingly, it is recommended to start heating on November 15 for the production of off-season (December) asparagus in Jeju. Therefore, the optimum heating date to start was November 15 for the improvement of quality and yield of off-season (December) asparagus in Jeju area.

• Journal of Korean Society of Forest Science
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• v.108 no.4
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• pp.531-539
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• 2019

This study assessed the vitality of Carpinus laxiflora seeds stored for more than 15 years in order to discover optimal pre-treatment conditions for removingthe causes of seed dormancy and improving revitalization rate. Seeds were collected in October 2000 and stored at -18℃ for 16 years. Experiments to assess the revitalization ability of the seeds were performed under the following conditions: a controlled environment; in warm stratification (30 days at 23℃); in cold stratification (30 days at 4℃ andfor 30, 45, 60, and 120 days); gibberellin (GA₃) treatment (24 hours per day in GA₃ solutions of 100, 500, 1000, 2000, and 3000 mg·L^-1); and both cold stratification and GA₃ treatment (30 days at 4℃, then in a GA₃ solution of 100, 500, and 1000 mg·L^-1 for 2 hours). The average germination percentage(GP) of untreated seeds was 2%, and the average GP of warm-stratification seeds was 10%. Cold-stratification seeds had the highest GP at 81% for the 45-day process, while the 120-day cold-stratification seeds had the lowest GP at 67.3%. The average GP of seeds treated with GA₃ ranged from 77% (100 mg·L^-1) to 99% (1000 mg·L^-1), indicatingsignificant differences between the treatment concentrations. The treatment effect of GA₃ was highest at 500 mg·L^-1 and 1000 mg·L^-1, and lowest at 100 mg·L^-1. The average GP of seeds treated with GA₃ following cold stratification was 68%, which was lower than the cold stratification-only (73.2%) and GA₃-only (88.4%) treatments. A comprehensive comparison of the seed germination characteristics according to the four treatments determined that a GA₃ 500 mg·L^-1 pre-treatment, with the highest average GP, was ideally suited to the revitalization of long-term stored C. laxiflora seeds. Consequently, C. laxiflora stored at -18℃ for 16 years indicated strong vitality and could be regenerated by proper pre-treatment.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.54 no.4
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• pp.384-389
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• 2009

Germination characteristics of Chinese milk vetch(CMV) seeds produced from Hanam Province, China and Milyang, Korea were investigated to give basic information on the stability of seedling establishment in the CMV cultivation. The germination percentage of the imported CMV seed from China varied according to importation year and seed collection site ranging from 79~95%. The germination of black colored seed coat was lower than the light green colored ones and germination by seed weight was not significantly different. Although the seed germination was lower under dark than in the presence of light, it was not significantly different. The germination of the imported CMV seed slightly declined to only less than 6% after one-year of storage under natural environment conditions but it significantly decreased after two years. However, when the seed was stored at the $5^{\circ}C$, the seed germination was the same as after two years of storage. On the other hand, fresh CMV seed produced in Milyang, Korea had only 8% germination due to seed coat dormancy but the germination increased to 73~85% after breaking seed dormancy after a year of storage. The high germination percentage of 72~82% was still maintained even after 27 months of seed storage unlike the CMV seed produced from China. These results indicate that CMV seeds do not require light for germination and the seed from China should be used within one-year after importation while the seeds produced from Korea can be used even after two years from harvest for stable CMV seedling establishment in the CMV-rice cropping system.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.32 no.3
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• pp.277-286
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• 1987

Abscisic acid (ABA) content of the seed and endocarp during stratification were analyzed and then examined in relation to the embryo growth and germination. In mature red fruitlet, the ABA content was remarkably higher in sarcocarp than those in both seed ans endocarp. During the stratification before dehiscence, ABA content was gradually decreased in both seed and endocarp. After 90 days(dehiscent percentage; 96%) it came to 90 pmol/ g DW(69% decrease) and to 41 pmol/ g DW (80% decrease) in seed and in endocarp, respectively. The ratio of free from to total ABA content showed constant decrease in seed, but remained at higher level in endocap than in seed. Correlation between the decrease of ABA content and embryo growth showed higher significance in seed than in endocarp. During the stratification after dehiscence, ABA content in seed was gradually decreased at 4$^{\circ}C$ and 15$^{\circ}C$, After 90 days it came to 28 pmol/ g DW (69% decrease) and to 46 pmol/ g DW (49% decrease) at 4$^{\circ}C$ and at 15$^{\circ}C$, respectively. The ratio of free form to total ABA content was gradually increased at 4$^{\circ}C$, but remained almost constant at 15$^{\circ}C$. Correlation between the decrease of ABA content and days to first germination showed positive singificance only at 4$^{\circ}C$, whereas the correlation between the decrease and mean germination percentage per day showed negative significance at 4$^{\circ}C$, but positive significance at 15$^{\circ}C$. The above results indicate the ABA of the seed end endocarp during the stratification before dehiscence seems to be concerned with the immature embryo growth, but that of the seed during the stratification after dehiscence seems to show little effect on the germination capability(degree of breaking physiological dormancy).

• Journal of Bio-Environment Control
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• v.32 no.2
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• pp.157-164
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• 2023

Codonopsis lanceolata (S. et Z.) Trautv. is mainly cultivated in Korea and China as a medicinal crop. C. lanceolata is difficult to produce plug seedlings in the summer, because C. lanceolata has a low germination rate and is vulnerable to high temperatures. Cold treatment is effective in breaking dormancy of seeds and increasing the germination rate. Shading cultivation can control the solar irradiance received by plants and reduce the damage by high temperatures and strong light. This study was conducted to examine the appropriate cold treatment period for the improving germination of C. lanceolata, and shading level during the summer seedling period. Cold treatment experiments were conducted for 0 (control), 1, 2, 3, and 4 weeks at 4℃ before sowing. In the shading experiment, C. lanceolata was grown for 45 days with 0 (non-treatment), 45, 75% shading levels. Cold treatment for one week significantly improved the germination energy. The plant height, leaf area, and fresh and dry weights of C. lanceolata seedlings were significantly increased under the 45% shading level. Total root length, root surface area, and the number of root tips were significantly higher in shading treatment (45 and 75%) than in non-treatment. The C. lanceolata seedling's compactness and Dickson's quality index were the highest at 45% shading level. Therefore, these results recommended sowing C. lanceolata after cold treatment for one week at 4℃, and 45% shading level could stably culture C. lanceolata plug seedlings during the high temperature period.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.60 no.3
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• pp.388-393
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• 2015

Buckwheat sprouts are a vegetable; a functional food should provide health benefit and enhance performance as high nutritionally important substances. Buckwheat noodles are the major buckwheat food in Japan, Korea and China. In addition, Buckwheat as preventive medicine has undergone a great advancement in the last decade. Comparison of the functional properties distribution and utilization in tatary buckwheat is required of understanding the metabolites. The study was conducted to identify the sorts of phenolic compounds and metabolites in tatary buckwheat seedling at 4, 7, and 10 days seedling under the different combinations of light-emitting diode (LED) such as blue, red, mix (red, blue, and white), dark, and natural lights in stem and leaves. After breaking the dormancy, buckwheat seeds were grown in culture room under lights for 14 hrs and the dark condition for 10 hrs, at $25^{\circ}C$ for 10 days. Length of buckwheat was gradually increased under all of the conditions. Using HPLC, rutin was highest at 7 days under mix and natural light in stem and leaf, respectively. Quercetin was highest at 4 and 7 days under natural light in both. Chlorogenic acid was highest at 7 days under mix and natural in stem and leaf, respectively. Taken Together, this study indicates that phenolic compounds and metabolites present in those plants could be helpful for the human health and nutritional additive.

• Korean Journal of Environment and Ecology
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• v.30 no.3
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• pp.308-319
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• 2016

The purpose of this study is to identify distribution patterns of hydrophytes in the Mokpo wetland in relation to the depth of water. Sample surveys were conducted based on plant species distribution status and water depths at 274 spots. This study also developed a detailed depth distribution map for Mokpo wetland, which was never done in any previous studies. Through this study, it was found that the average depth of the wetland was 77cm (${\pm}29cm$) and the maximal depth was 157cm. The outer edge was no deeper than 60cm and the center approximately 120~130cm in depth, forming a concave bowl-like shape. This research confirmed inhabitation of 6 types of submerged plants (Verticillate hydrilla, Vallisneria natans, Najas graminea, Potamogeton cripus, Ceratophyllum demersum, and Potamogeton brechtoldi), and three types of floating leaved plants (Euryale ferox, Hydrocharis dubia and Trapa japonica) in the surveyed areas of the wetland. The distribution of these hydrophytes showed a statistically significant difference (${\chi}^2=982.2$, df = 8, p < 0.01), which confirms the fact that distribution varies based on environmental conditions. The most frequently observed species was Trapa japonicas at 244 times, and it showed a distribution pattern by which coverage increased with greater depth, as was also seen in the case of Potamogeton cripus. Five species-Euryale ferox, Hydrocharis dubid, Verticillate hydrilla, Najas graminea, and Ceratophyllum demersum-showed a negative correlation to depth, by which coverage decreased with increasing depth. It has been shown that fundamentally, the distribution of hydrophytes based on depth is affected by ecological factors, but also reflects the environmental properties of Mokpo wetland.

• Journal of Bio-Environment Control
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• v.21 no.1
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• pp.45-49
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• 2012

Chilean wild flower seeds showed low germination rate and lacked germination uniformity. This experiment was conducted to find out the effects of light conditions, $GA_3$ (50 mg/L, 100 mg/L, 200 mg/L) and $KNO_3$ (0.1%, 0.2%, 0.4%) treatments on germination of five species of Chilean wild flower seeds. The seeds of $Schizanthus$ $candidus$, $Schizanthus$ $hookerii$, and $Schizanthus$ $litoralis$ did not germinated in light condition. The others did not show a significant different germination rate under light and dark conditions. The germination rate of $Mimulus$ $luteus$, $Schizanthus$ $candidus$, $Schizanthus$ $hookerii$ increased 2.3, 5.6, and 3.2 times compared with control, respectively in $GA_3$-200 mg/L treatment, but that of $Rhodophiala$ $ananuca$ enhanced slightly in $GA_3$-50 mg/L treatment. Those results suggested that the germination rate of Chilean wild flower seeds enhanced by $GA_3$ treatment that is very efficient in breaking seed dormancy.

• Korean Journal of Medicinal Crop Science
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• v.6 no.2
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• pp.102-107
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• 1998

Solomon's seal (Polygonatum odoratum) seedlings raised through its seeds can replace the rhizomes impelling higher cost for transplanting, This experiment was done to determine the seed characteristics and the germinating processes to give some information on bulk production of seedlings using the seeds. The external or internal morphology of seeds or seedlings grown in lab. or greenhouse was examined mainly with stereomicroscope. The external shape of Solomon's seal seed was hard seed-coat and orthotropous ovule with linear type embryo stretching to the center of seed. Germination proceeded through the several steps. The lower part of seed embryo having the primordia of bulbil and roots first grew before the bulbil and roots was developed from the primordia. The lower part of embryo was enlarged toward the endosperm of seed as soon as seed germinated. Then epicotyl was formed on the apex of bulbil. The epicotyl was elongated after at least 6-week chilling treatment for breaking its dormancy and the first leaf shape was affected by light intensity given during seedling emergence. The bulbil was the first organ of the rhizome used as tea or herb medicine.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.15 no.2
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• pp.103-115
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• 2012

To investigate the attributes of seed germination of the plants for greening of river bank slope, low-temperature treatment, chemical treatment, plant hormones treatment, and stratification were conducted using the seeds of 6 herbs growing naturally around river banks, such as Setaria glauca and faberii, Imperata cylindrica var. koenigii, Miscanthus sinensis, Pennisetum alopecuroides, Lespedeza cuneata and the seeds of 3 shrubs, such as Lespedeza bicolor., Rosa multiflora, Spiraea prunifolia for. simpliciflora. The findings are as follows. In case of herbs, Setaria glauca and faberii. have stone seeds. In the treatment with $H_2SO_4$, they were germinated through dormancy breaking, even though there were some differences depending on immersion time, and the germination rate of Setaria faberii was a bit higher than that of Setaria glauca. The germination rate of Imperata cylindrica var. koenigii was low in the treatment with $GA_3$, NAA, and the plant hormone, but the seed germination rate has improved and the germination rate of Miscanthus sinensis and Pennisetum alopecuroides showed the improved seed germination rate in low temperature treatment. In case of Lespedeza cuneata and bicolor, the leguminous plants, the treatment with $H_2SO_4$ was most effective for the improvement of seed germination and the decrease in germination time. Only Spiraea prunifolia for. simpliciflora, the rose among 3 woody plants showed the seed germination rate above 50% without any additional treatment. Thus, it was thought to be appropriate for greening. Rosa multiflora showed the attribute of germination in a dark condition at low temperature through stratification, despite its low germination rate. Based on the results as above, with respect to the greening using directly collected seeds, Miscanthus sinensis, Pennisetum alopecuroides, and Spiraea prunifolia for. simpliciflora are suitable for greening, because they do not need any additional treatment, and Lespedeza cuneata and bicolor are also suitable for greening because it is possible to improve the germination rate and reduce the germination time through the treatment on the seeds before sowing.