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Relationship between Molecular Structure of Rice Amylopectin and Texture of Cooked Rice (쌀의 아밀로펙틴 분자구조와 밥의 텍스쳐)

  • Kang, Kil-Jin;Kim, Kwan;Kim, Sung-Kon
    • Korean Journal of Food Science and Technology
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    • v.27 no.1
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    • pp.105-111
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    • 1995
  • The relationship betwwen the molecular structure of amylopectin and the texture of cooked rice was investigated using Korean rice [3 varieties of Japonica type and 3 varieties of Tongil type(Japonica-Indica breeding type)]. The molecular structure of rice amylopectin was polymodal and distributed A chain of $\overline{DP}$ 12.4, short B chain of $\overline{DP}$ 20.6, B chain of $\overline{DP}$ 26.3, long B chain of $\overline{DP}$ 45 and super long chain of above $\overline{DP}$ 55. The super long chain of amylopectin was composed of long linear chain with poorly branched chain. Also, the super long chain of amylopectin showed positive correlated with average chain length, inherent viscosity and ${\beta}-amyloysis$ limit$({\%})$, but negative correlated with ${\lambda}max$ of iodine reaction of amylopectin. The structural properties of amylopectin in Japonica type were different from those of amylopectin in Tongil type. In relationship between molecular structure of amylopectin and texture of cooked rice, the average chain length, inherent viscosity, ${\beta}-amyloysis$ limit and super long chain of amylopectin was showed a positive correlation with hardness, but a negative correlation with adhesiveness of cooked rice. The long chain of rice amylopectin is the less, the eating quality of cooled rice was the better. These results suggest that the molecular structure of rice amylopectin could be responsible for the texture of cooked rice.

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An Analysis of the Verbal Interaction Patterns of Science-Gifted Students in Science Inquiry Activity (과학 탐구 활동에서 나타나는 과학영재들의 언어적 상호작용 유형 분석)

  • Kim, MyungHee;Kim, Youngshin
    • Journal of The Korean Association For Science Education
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    • v.35 no.2
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    • pp.333-342
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    • 2015
  • This study analyzes the verbal interaction patterns used in a social network activity analysis that appeared in a science inquiry activity of 31 small groups of science-gifted students consisting of 5 members each. The results of this study are as follows: The interaction patterns showed eight types. The most prevalent interaction pattern, type 1, is triangle-shaped, interacting with 3 members out of 5 without a central member. Type 2 is wye form, interacting with 4 members and with one alienated member. Type 3 is diamond-shaped, interacting with 4 members. Type 4 is ray form, interacting with 5 around a central member. Type 5 has an alienated member and interacts with 4 members around the central member. Type 6 is triangle-branched, 4 members linked to the central member. Type 7 is wye form linked all around the central member. Type 8 is wye form with a more complex link than type 7. These can be classified in two. One is the participation-type where the rest of the 4 members are linked to the central member. The other is the alienation-type where a member/members is/are alienated without a central member. The participation-type appeared in 9 groups (29%), type 4, type 6, type 7, and type 8. The alienation-type showed in 22 groups (71%), type 1, type 2, type 3, and type 5. On the basis of this study, we propose that the best number of members in a group is three. It helps prevent a free-riding effect or isolation of members. Also, we deem it more fruitful if there is a member playing a central role in a group.

Trichome Type and Development in Leaves of Althaea rosea (접시꽃 (Althaea rosea) 엽육표피에서의 모용의 분화 발달)

  • Kim, In-Sun;Lee, Seung-Hee
    • Applied Microscopy
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    • v.35 no.2
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    • pp.97-104
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    • 2005
  • Plant epidermis consists of relatively unspecialized cells and more specialized cells of various structure and function. Trichomes are specialized cells originated from the epidermis and much attention has been paid to the plants developing trichomes with peculiar structure and function. The present study has been undertaken to examine the trichome type noticed in the leaf epidermis of Althaea rosea using scanning electron microscopy. Four types, namely simple, short-and long-tufted, and glandular hairs, were detected in their epidermis. Their Distribution, frequency and structure varied by the development and epidermal surface. The most frequently distinguished type was the tufted ones growing in young leaves of the abaxial epidermis, while the simple hairs were rare throught the examination. The short-tufted hairs branched up to seven times having each branchlet about $160{\sim}210{\mu}m$ in length at maturity. The long-tufted hairs exhibited up to ten branchlets, where branchlets could reach up to $900{\sim}1,000{\mu}m$ long when fully expanded. Glandular trichome was the peltate type comprising $1{\sim}2$ secretory head cells, 2 stalk cells and a basal cell. The short peltate glandular hairs, usually not exceeding $40{\mu}m$, differentiated more along the areoles in the adaxial epidermis. The function of these trichomes in A. rosea has been still obscure, but it has been speculated that they probably play a role in protection; non-glandular ones possibly providing a defense against insects and secretory glandular type participating in chemical defense. Structural features of these trichomes at cellular level will be discussed in the following study of transmission electron microscopy.

Crystal Structure of the Three-dimensional Metal Complex Host in Clusion Compound [$Cd(pn)Ni(CN)_4{\cdot}0.5(CH_3COCH_3{\cdot}H_2O$) (3차원 금속 착제를 Host로 하는 포접 화합물 [$Cd(pn)Ni(CN)_4{\cdot}0.5(CH_3COCH_3{\cdot}H_2O$)의 결정구조)

  • Park, Gi Min;Lee, Uk;Am, Bon Jin Mu
    • Journal of the Korean Chemical Society
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    • v.38 no.6
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    • pp.435-441
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    • 1994
  • The inclusion compound constituted with three-dimensional metal-complex $Cd(pn)Ni(CN)_4$ has been prepared and determined the crystal structure from single crystal X-ray diffraction data. Crystal data are as follows: $[Cd(pn)Ni(CN)_4]{\cdot}0.5(CH_3COCH_3{\cdot}H_2O)$, Fw = 387.35, Orthorhombic, $Pn2_1a$, a = 13.950(3) $\AA$, b = 26.713(7) $\AA$, c = 7.628(1) $\AA$, V = 2843(1) $\AA^3$, Z = 4, $D_x=1.81 gcm^{-3}$, $\mu(MoK{\alpha})$ = $28.153 cm^{-1}$, T = 297K, final R = 0.0418 for 3521($F_0>3{\sigma}(F_0)$). The metal-complex host provides tunnel cavity, similar to thiourea inclusion compounds, accommodated guest molecules $(=CH_3COCH_3\;and\;$H_2O).$ The stoichiometric host: guest ratio is 0.5. The title inclusion compound reveals another evidence for the host-selectivity, that is, the branched aliphatic guest molecule leads T-type host structure.

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Prediction of Retention Indices of Various Compounds in Gas-Liquid Chromatography (기체-액체 크로마토그래피에서 화합물의 머무름 지표의 예측에 관한 연구)

  • Cha, Gi Won;Lee, Deok Jae
    • Journal of the Korean Chemical Society
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    • v.38 no.2
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    • pp.108-121
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    • 1994
  • The retention indices of branched-chain alkane, benzene ring, alcohol, amine, ketone, aldehyde and cyclic compounds were measured at 150, 180 and $210^{\circ}C$ on OV-1701 and OV-1 capillary columns. The group retention factors (GRF) of the substituents and the st` ructure retention factors (SRF) of the molecular structure change are derived from the retention indices of reference compounds and series of homologues. The $GRF_f$ equation of `f'th substituent is $GRF_f\;=\;I_{obs}-(100Z + \sum\limits_{i{\neq}f}GRF_i$ + {\sum}$SRF_i$)and the SRFf equation of `f'th molecular structure group is $SRF_f\;=\;I_{obs}-(100Z + {\sum}GRFi + \sum\limits_{i{\neq}f}SRF_i$). The predicted retention indices for those compound were in agreement, within the error of $\pm2$ and $\pm3%$, with the observed values that were obtained using the OV-1701 and OV-1 capillary column, respectively. The $\Delta$ xi of the substituents and $\Delta$ yi of the molecular structure change according to temperature change are derived from the $\Delta'/^{\circ}C$ of reference compounds and series of homologues. The $\Delta$ xi equation of the `f'th substituent is ${\Delta}x_f = {$\Delta}'/^{\circ}C+ \sum\limits_{i{\neq}f}\Delta$ xi + {\sum}{\Delta}yi\;and\;{\Delta}yi$ equation of the `f'th molecular structure group is ${\Delta}y_f$ = {\Delta}'/^{\circ}C+{\sum}{\Delta}xi +\sum\limits_{i{\neq}f}{\Delta}yi$. The predicted $\Delta'/^{\circ}C$ for these compounds were in agreement, within the error of ${\pm}18%$ and 17%, with the observed values that were obtained using the OV-1701 and OV-1 capillary column, respectively.

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Effects of red ginseng supplementation on aerobic.anaerobic performance, central and peripheral fatigue (홍삼 섭취가 유.무산소성 운동수행능력과 중추 및 말초피로에 미치는 영향)

  • Yoon, Sung-Jin;Kim, Ki-Hyung;Kim, Chang-Ju;Park, Hae-Chan;Kang, Kyung-Hee;Kim, Moo-Jung;Kang, Sung-Mok;Kwak, Uk-Heon;Kim, Hyung-Joon
    • Journal of Ginseng Research
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    • v.32 no.3
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    • pp.210-219
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    • 2008
  • The purpose of this study is to examine the effect of red ginseng on the performance of aerobics anaerobics exercise and peripheral central fatigue. For this purpose, we measured $\dot{V}O_2max$, $\dot{V}O_2$ at recovery, anaerobic power, blood lactate, Branched-Chain Amino Acid(BCAA) etc in thirty male subjects. They were divided into three group; EGG: endurance training + red ginseng supplementation group(n=7), EPG: endurance training + placebo supplementation group(n=7), GG: only red ginseng supplementation group(n=10). 6 subjects were excepted because they were intermediate test absence(test at four week : EGG=1, EPG=2; test at eight week : EGG=1). EGG and GG took red ginseng twice a day for total 8weeks. Moreover, EGG and EPG were exercised during 45 minutes, three times a week for 12 weeks. The result of this study are shown as follow: In case of $\dot{V}O_2max$, %$\dot{V}O_2$/$\dot{V}O_2max$ and plasma BCAA concentration, no statistically significant change were found among three groups. But, it was showed that three groups tended to increased slightly in $\dot{V}O_2max$, % $\dot{V}O_2$/$\dot{V}O_2max$ and plasma BCAA concentration. For conclusion, the present data provide a new evidence that ingestion of ergogenic aids contained with red ginseng may improve aerobic exercise performance and central fatigue.

A New Species of Cellular Slime Molds from Korea, Dictyostelium flavidum sp. nov. (한국산 세포성 점균의 1 신종)

  • 홍정수
    • Journal of Plant Biology
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    • v.35 no.3
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    • pp.197-203
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    • 1992
  • During the study of the distribution of cellular slime molds in Halla mountain of Korea, a new yellow-pigmented Dictyostelium was isloated. This exibited several distinctive features which differed from the published species, and was designated as a new species, Dictyostelium jlavidum sp. n. Hong et Chang (Type strain HL-1). It was cultivated at $20-22^{\circ}C$ on weak nutrient agar media, 0.1 L-P in association with Escherichia coli. Sorocarps were 4-10 mm or more in length, conspicuously yellow throughout or with sori, typically solitary, unbranched or sparsely and irregularly branched. Sorophores were strongly tapered from bases to tips. Bases were typically well-formed disks in form or conically expanded. Sori were yellow to yellowish in color, and the pigmentation intensified with age. Spores were long and thin elliptical, mostly $4.8-9.6{\times}1.9-3.8\;(ave.\;7.3{\times}2.8)\;\mu\textrm{m}$, L/B index about 2.4-2.8, without polar granules.anules.

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Multicotyledonary Structure of Somatic Embryos Formed from Cell Cultures of Daucus carota L. (당근의 배양세포로부터 형성된 체세포배의 다자엽 구조)

  • 소웅영
    • Journal of Plant Biology
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    • v.39 no.1
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    • pp.71-77
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    • 1996
  • Embryogenic callus was selected from callus induced from hypocotyl segment cultures of Daucus carota seedlings on MS basal medium supplemented with 1.0 mg/L 2,4-D. Cell clumps prepared from the embryogenic callus were transferred to MS medium without 2,4-D for somatic embryo development. Cotyledonary abnormalities were frequently observed on somatic embryos developed after the incubation of cell clumps in MS basal medium with 1.0 mg/L 2,4-D for one week and then subculture in the same medium but without 2.4-D for two weeks. The percentage of abnormalities was as follows: 5% one cotyledon, 21% three cotyledons, 6% four cotyledons, 5% five cotyledons, 0.2% six cotyledons and 1% trumpet-like cotyledons. On the other hand, the normal somatic embryo with two cotyledons appeared at 63%. The germination rate of somatic embryos was higher in two cotyledon somatic embryos than in multicotyledonary embryos. Trumpet-like somatic embryos did not germinate normally showing limited elongation and enlargement of roots and cotyledons without shoot development. From anatomical examination circular procambium in the root of somatic embryo began to branch around the middle regions of the hypocotyl which extended into the cotyledons through the cotyledonary nodes and the number of branched procambial strands in hypocotyl was equal to the number of cotyledons. Monocotyledonous somatic embryo always had larger cotyledon than that of somatic embryos with multicotyledons and had horseshoe-like cotyledons where the procambium was of the same structure.ucture.

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Gray Mold Rot on Fruit of Cucumis melo var. reticulatus Caused by Botrytis cinerea (Botrytis cinerea에 의한 멜론 잿빛곰팡이병)

  • Kwon, Jin-Hyeuk;Kang, Soo-Woong;Son, Kyeng-Ae;Bae, Dong-Won;Park, Chang-Seuk
    • The Korean Journal of Mycology
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    • v.27 no.4 s.91
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    • pp.280-282
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    • 1999
  • A new disease on mask melon grown under plastic film houses was found in Namhae area in May of 1999. Gray to dark brown mold were grown on the surface of matured fruits and infected inside tissues were discolored and rotten. Basal part of the fruit and blossom-end were frequently infected and colonized by fungi. About 2.2% of matured fruits were infected in the surveyed plastic film houses. The causal organism was isolated from the lesion and identified as Botrytis cinerea. The conidia in mass were hyaline or gray, 1-celled, mostly ellipsoid or ovoid and sized $8.8{\sim}21.2{\times}6.5{\sim}13.1\;{\mu}m$. Hyaline or pigmented conidiophores were tall, slender and determinated and, sometimes branched irregularly in upper part. Enlarged or rounded apical cells bear conidial cluster and sized $18.4{\sim}81.1{\times}4.3{\sim}11.4\;{\mu}m$. Optimum temperature for mycelial growth was recorded at $15{\sim}25^{\circ}C$. This is the first report on gray mold of melon caused by Botrytis cineria in Korea.

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Optimization of Growth Medium Composition for Overproduction of Bacillus licheniformis Amylase in Recombinant Escherichia coli (Bacillus licheniformis amylase(BLMA)의 생산성 향상을 취한 재조합 대장균의 배지 최적화)

  • Nam, Seung-Hun;Lee, Woo-Jong;Byun, Tae-Gang;Seo, Jin-Ho;Park, Kwan-Hwa
    • Korean Journal of Food Science and Technology
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    • v.26 no.4
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    • pp.411-416
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    • 1994
  • The research is concerned with optimization of growth medium composition in an attempt to improve the product yield of Bacillus licheniformis amylase (BLMA) in recombinant E. coli containing the BLMA gene. BLMA has the catalytic activity of producing branched oligosaccharides from starch. The medium optimization was performed in flask cultures based on the Box and Wilson method. The optimized medium is composed of tryptone 18.0 g/l, yeast extract 22.4 g/l, NaCl 5.3 g/l and glucose 2.1 g/l. In a jar fermenter culture with the conventional LB medium, the recombinant E. coli yielded 1.39 g/l of final dry cell mass and 5.11 U/ml of enzyme activity. In the optimized medium, however, the final cell mass was increased to 6.01 g/l and the enzyme activity to 23.2 U/ml. Medium optimization improved cell mass by 4.3 times and enzyme activity by 4.5 times. Such an increase in enzyme activity is mainly due to an enhancement of cell mass.

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