Korean Journal of Food Science and Technology (한국식품과학회지)
- Volume 26 Issue 4
- /
- Pages.411-416
- /
- 1994
- /
- 0367-6293(pISSN)
Optimization of Growth Medium Composition for Overproduction of Bacillus licheniformis Amylase in Recombinant Escherichia coli
Bacillus licheniformis amylase(BLMA)의 생산성 향상을 취한 재조합 대장균의 배지 최적화
- Nam, Seung-Hun (Department of Food Science and Technology and Research Center for New Biomaterials in Agriculture, Seoul National University) ;
-
Lee, Woo-Jong
(Department of Food Science and Technology and Research Center for New Biomaterials in Agriculture, Seoul National University) ;
-
Byun, Tae-Gang
(Department of Food Science and Technology, Keonyang University) ;
-
Seo, Jin-Ho
(Department of Food Science and Technology and Research Center for New Biomaterials in Agriculture, Seoul National University) ;
-
Park, Kwan-Hwa
(Department of Food Science and Technology and Research Center for New Biomaterials in Agriculture, Seoul National University)
- 남승헌 (서울대학교 식품공학과 및 농업생물신소재연구센터) ;
-
이우종
(서울대학교 식품공학과 및 농업생물신소재연구센터) ;
-
변태강
(건양대학교 식품공학과) ;
-
서진호
(서울대학교 식품공학과 및 농업생물신소재연구센터) ;
-
박관화
(서울대학교 식품공학과 및 농업생물신소재연구센터)
- Published : 1994.08.01
Abstract
The research is concerned with optimization of growth medium composition in an attempt to improve the product yield of Bacillus licheniformis amylase (BLMA) in recombinant E. coli containing the BLMA gene. BLMA has the catalytic activity of producing branched oligosaccharides from starch. The medium optimization was performed in flask cultures based on the Box and Wilson method. The optimized medium is composed of tryptone 18.0 g/l, yeast extract 22.4 g/l, NaCl 5.3 g/l and glucose 2.1 g/l. In a jar fermenter culture with the conventional LB medium, the recombinant E. coli yielded 1.39 g/l of final dry cell mass and 5.11 U/ml of enzyme activity. In the optimized medium, however, the final cell mass was increased to 6.01 g/l and the enzyme activity to 23.2 U/ml. Medium optimization improved cell mass by 4.3 times and enzyme activity by 4.5 times. Such an increase in enzyme activity is mainly due to an enhancement of cell mass.
유전자 재조합 대장균에서 분지올리고당을 생산하는 Bacillus licheniformis amylase(BLMA)효소의 생산수율을 향상시키기 위하여 배지최적화 연구를 수행하였다. 기존 LB배지로 5l 발효조에서 배양한 결과 재조합 균주인 E. coli pIJ322의 최대비생육속도(