• 제목/요약/키워드: brain development

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심장과 뇌 발달에서 GATA6 유전자 발현 감소가 미치는 영향 (The Effects of the Expression of GATA Binding Protein 6 on Heart and Brain Development)

  • 서정원
    • 생명과학회지
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    • 제25권11호
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    • pp.1230-1234
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    • 2015
  • GATA binding protein 6 (GATA6)는 초기 배반포 단계에서 발현이 시작되어, 심장, 췌장, 장 등의 분화와 발달에 중요한 유전자 발현을 조절하는 전사인자이다. 본 연구에서는 GATA6의 세포 분화와 개체 발달 과정에서의 역할을 마우스 배아줄기세포와 zebrafish를 이용하여 확인하였다. 먼저, 마우스 배아줄기세포를 박동하는 pacemaker 심근세포로 분화 유도하였다. RT-PCR을 실시하여 심근세포 분화 과정에서 GATA6 유전자 발현 변화를 확인한 결과, Gata6의 발현이 분화 4일째부터 증가함을 확인하였다. GATA6 유전자의 발현 증가는 심장 발달에 필수적인 전사인자인 NK2 homeobox 5 (Nkx2.5)나 myocyte enhancer factor 2C (MEF2C)의 발현 증가에 앞서 나타났다. GATA6 유전자가 발달 과정에 미치는 영향을 확인하기 위하여 GATA6의 morpholino를 zebrafish 배아에 microinjection하여 발생 변화를 관찰하였다. GATA6의 발현을 knockdown시킨 zebrafish의 심장은 크기가 감소하였고, 심박동률 또한 감소하였다. 한편, 뇌에서는 전체적인 뇌 퇴행이 관찰되었는데, acridine orange로 염색 한 결과, 뇌 전체에서의 세포사멸의 증가를 나타내었다. 흥미롭게도, GATA6의 발현 감소는 초기 bud 단계에서는 오히려 세포사멸을 감소시켰다. 본 연구는 심장과 뇌 발달에서의 GATA6 유전자의 중요성을 시사한다.

Tissue- and maturity-dependent expression pattern of androgen receptor mRNA in goldfish, Carassius auratus

  • Choi, Cheol-Young;Kim, Soon-Hag;Kim, Bong-Seok
    • 한국어병학회지
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    • 제16권2호
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    • pp.111-118
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    • 2003
  • Androgen plays an important role in the regulation of gonadotropin production in vertebrates . We have investigated the transcriptional pattern of androgen receptor (AR) in a variety of tissues in maturing male and female goldfish by RT-PCR. Specific primer for AR was designed based on goldfish AR gene from the GenBank (accession number AY090897). AR was shown 10 be maturity- and tissue-dependent gene expression pattern in goldfish. In immature male goldfish, significantly higher transcript level of AR was observed in the pituitary und testis , compared [0 brain and liver. Mature male goldfish showed a similar expression pattern to immature male goldfish. Interestingly. when compare to male goldfish, female goldfish showed AR mRNA expression that was found 10 be weak in pituitary, and very low expression in brain. They could not be found 10 have expression in any other tissues. Taken together. the- transcriptional analysis of AR depending on the tissue, sex. and maturity of a goldfish provides the opportunity for the study of goldfish reproductive physiology ,The results provided for the first time a comparison of the tissue distribution of AR mRNA in sexually maturating male and female goldfish.

발달 신경심리학 (NEUROPSYCHOLOGY IN NEURODEVELOPMENT)

  • 신민섭;김현미
    • Journal of the Korean Academy of Child and Adolescent Psychiatry
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    • 제16권1호
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    • pp.33-46
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    • 2005
  • 본고에서는 인간의 뇌 발달과 주의력, 지각, 기억, 언어 등 다양한 인지기능의 발달과의 관계를 살펴본 후, Luria의 발달 신경심리 이론 및 그에 입각하여 개발된 아동용 Luria-Nebraska신경심리검사를 간략하게 소개하였다. 마지막으로, 현재 국내, 외에서 널리 사용되는 주의력 검사, 시-지각 검사, 시각-운동 협응 능력 검사, 그리고 전두엽의 집행기능검사 등을 포함한 아동용 신경심리 평가도구에 대해 고찰해보았다.

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인공지능 뉴로모픽 반도체 기술 동향 (Trend of AI Neuromorphic Semiconductor Technology)

  • 오광일;김성은;배영환;박경환;권영수
    • 전자통신동향분석
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    • 제35권3호
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    • pp.76-84
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    • 2020
  • Neuromorphic hardware refers to brain-inspired computers or components that model an artificial neural network comprising densely connected parallel neurons and synapses. The major element in the widespread deployment of neural networks in embedded devices are efficient architecture for neuromorphic hardware with regard to performance, power consumption, and chip area. Spiking neural networks (SiNNs) are brain-inspired in which the communication among neurons is modeled in the form of spikes. Owing to brainlike operating modes, SNNs can be power efficient. However, issues still exist with research and actual application of SNNs. In this issue, we focus on the technology development cases and market trends of two typical tracks, which are listed above, from the point of view of artificial intelligence neuromorphic circuits and subsequently describe their future development prospects.

Entrepreneur in Academic Research: Interview with Professor Kwang-Hyung Lee

  • Seol, Sung-Soo;Suh, Sanghyuk
    • Asian Journal of Innovation and Policy
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    • 제5권3호
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    • pp.330-342
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    • 2016
  • This is an interview with Professor Kwang-Hyung Lee, founding Dean of KAIST Moon Soul Graduates School of Future Strategy and founder of the Department of Bio and Brain Engineering of the same university casting two questions about academics. The first question is what pattern is desirable in the evolution of research topics of an academics. While traditional researchers in science and engineering tend to focus on one subject in ever greater depth over time, Professor Lee's research agenda has spanned several new topics by gradually changing the content of the study: from artificial intelligence to bio and brain research, and to creativity development method, further to future study. The second question is about researchers' social responsibility. He has devoted to contributes to industry fields and the nation through academic activities as well as educating several successful business people, founding a new academic department and graduate school of future studies.

The Alpha Subunit of Go Interacts with Promyelocytic Leukemia Zinc Finger Protein

  • Ghil Sung-Ho
    • 대한의생명과학회지
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    • 제10권4호
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    • pp.407-413
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    • 2004
  • Heterotrimeric GTP binding proteins (G proteins) transduce signals of a variety of hormones and neurotransmitters. Go is one of the most abundant G proteins in the brain and classified as the Gi/Go family due to their sequence homology to Gi proteins. While the Gi proteins inhibit adenylyl cyclase and decrease the intracellular cAMP concentration, the functions of Go is not clearly understood despite their sequence homology to Gi. The promeylocytic leukemia zinc finger protein (PLZF) is a DNA binding transcription factor and is expressed highly in central nervous system (CNS). Several studies reported that PLZF may be involved in regulation segmentation/differentiation during CNS development. Here, I report that the alpha subunit of Go (Go ) interacts with PLZF. The interaction between Goa and PLZF was verified by using GST pulldown assay and co-immunoprecipitation. Our findings indicate that Goa could modulate gene expression via interaction with PLZF during neuronal or brain development.

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The Catalytic Subunit of Protein Kinase A Interacts with Testis-Brain RNA-Binding Protein (TB-RBP)

  • ;길성호
    • 대한의생명과학회지
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    • 제13권4호
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    • pp.305-311
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    • 2007
  • cAMP-dependent protein kinase A (PKA) is the best-characterized protein kinases and has served as a model of the structure and regulation of cAMP-binding protein as well as of protein kinases. To determine the function of PKA in development, we employed the yeast two-hybrid system to screen for catalytic subunit of PKA $(C\alpha)$ interacting partners in a cDNA library from mouse embryo. A Testis-brain RNA-binding protein (TB-RBP), specifically bound to $C\alpha$. This interaction was verified by several biochemical analysis. Our findings indicate that $C\alpha$ can modulate nucleic acid binding proteins of TB-RBP and provide insights into the diverse role of PKA.

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흰쥐 태아 뇌에서 GnRH 신경세포의 초기발생과정 (Prenatal Development of Gonadotropin Releasing Hormone (GnRH) Neurons in the Rat Brain)

  • 이영기;최완성
    • 한국동물학회지
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    • 제34권4호
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    • pp.491-499
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    • 1991
  • The present experiment was carried out 1) to study the developmental topography of GnRH neuronal system and 2) to characterize the cellular localization of GnRH neurons in the prenatal brain development of the rat. At embryonic day (I) 14.5, immunoreactive cell bodies of GnRH were first seen in the nasal septum and in the ganglion terminate located in the ventral protion of the caudal olfactory bulb. Two days later (E 16.5), GnRH-containing neurons were observed at the level of olfactory tubercle and diagonal band of Broca, which is the first appearance in the intracerebral region. From 118.5, the topographic pattern of immunoreactive GnRH perikarya was similar to that of adult rats. The present data suggest that GnRH neurons were originated from the nasal septum and gradually extended to the hvpothalamic regions with increasing fetal age.

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Magnetic Resonance Imaging Meets Fiber Optics: a Brief Investigation of Multimodal Studies on Fiber Optics-Based Diagnostic / Therapeutic Techniques and Magnetic Resonance Imaging

  • Choi, Jong-ryul;Oh, Sung Suk
    • Investigative Magnetic Resonance Imaging
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    • 제25권4호
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    • pp.218-228
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    • 2021
  • Due to their high degree of freedom to transfer and acquire light, fiber optics can be used in the presence of strong magnetic fields. Hence, optical sensing and imaging based on fiber optics can be integrated with magnetic resonance imaging (MRI) diagnostic systems to acquire valuable information on biological tissues and organs based on a magnetic field. In this article, we explored the combination of MRI and optical sensing/imaging techniques by classifying them into the following topics: 1) functional near-infrared spectroscopy with functional MRI for brain studies and brain disease diagnoses, 2) integration of fiber-optic molecular imaging and optogenetic stimulation with MRI, and 3) optical therapeutic applications with an MRI guidance system. Through these investigations, we believe that a combination of MRI and optical sensing/imaging techniques can be employed as both research methods for multidisciplinary studies and clinical diagnostic/therapeutic devices.

Swimming During Pregnancy Increases the Expression c-Fos and c-Jun in the Hippocampus of Rat Offspring

  • Sim, Young-Je;Kim, Jee-Youn;Kim, Chang-Ju
    • 운동영양학회지
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    • 제13권1호
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    • pp.23-28
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    • 2009
  • The expression of c-Fos and c-Jun represents neuronal activity and plays a crucial role in the shaping of the development of brain. During the late pregnancy, exercise is known to influence neuronal activity of offspring. In the present study, the effect of swimming during pregnancy on the expression of c-Fos and c-Jun in the CA1, CA2, CA3 regions, and the dentate gyrus of the hippocampus of rat offspring was investigated using immunohistochemistry. Pregnant rats in the swimming group were forced to swim for 10 min once a day from 15 days after pregnancy until delivery. The expression of c-Fos and c-Jun in the CA1, CA2, CA3 regions, and the dentate gyrus of the hippocampus of pups was significantly increased by maternal swimming during late pregnant period. The present results show that prenatal swimming may enhance the neuronal activity of pups and affect the neonatal brain development.