• Horticultural Science & Technology
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• v.29 no.4
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• pp.326-335
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• 2011

When bougainvilleas are subjected to indoor low-light conditions, flower bracts regularly abscise. This study elucidates the effects of plant growth regulators on bract longevity of potted bougainvillea. Potted 'Taipei Red' bougainvillea in four different bract development stages were treated with 1-MCP (1-methylcyclopropene), NAA (1-naphthaleneacetic acid), SNA (sodium salt of naphthaleneacetic acid), IBA (indolebutyric acid), BA (6-benzylaminopurine), $KH_2PO_4$ (potassium dihydrogen phosphate), Put (diamine putrescine), SA (salicylic acid), or STS (silver thiosulfate) and were moved to indoor low-light conditions after treatments. Experimental results indicate that 1-MCP, NAA, SNA, BA, Put, and SA prolonged bract longevity, and this effect increased as bract stage increased. The effect of STS was significant in early bract stages and decreased as bract stages increased. Additionally, 1-MCP, NAA, SNA, BA, Put, SA, and STS treatment significantly reduced endogenous ACC (1-aminocyclopropene-1-carboxylate) content and ACC oxidase activity, suggesting that the inhibition of ethylene production was achieved via physiological metabolism. However, treatment with IBA or $KH_2PO_4$ had no effect on the bract longevity at any stage. In the combined chemical treatments, NAA + STS or NAA + SA were effectively for prolonging bract longevity and contained less protein or chlorophyll degradation, decrease ACC oxidase or ethylene production than the control. In conclusion, we propose that combined chemical treatment significantly prolonged the bract longevity and more effectively than single chemical treatment at any stage.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.5
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• pp.503-514
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• 1997

The morphogenesis of panicle and spikelet in paddy rice has been studied in high yielding Indica$\times$Japonica hybrid cultivar, Milyang 23 and a Japonica type cultivar, Koshihikari. Germinated seeds planted in $5000^{-1}$ a pots filled with submerged soil and cultured under natural conditions. The young panicle of main stem were continuously dissected and observered by Cryo-SEM from the panicle initiation stage until heading stage. Although the date of panicle differentiation and heading in Koshihikari earlier than those of Milyang 23. the sequence of panicle development in two cultivars begins when first bract primordium at opposite side of flag-leaf primordium differentiated, synchronously followed by growth of the primary branch primordia (PBPs) and secondary branch primordia (SBPs), spikelet primordia(SPs), glumes as lateral organs on rachilla and organs composing single floret, and successive sporogenesis in the young spikelets continue after the enclosure by lemma and palea. The PBPs are acropetally initiated from the base of the panicle primordium, and the SBPs alternately differentiate from the base of upper PBP which differentiate later than the lower PBP. Spikelet development starts at the top of upper side PBP of the young panicle and continue basipetally even though SBPs continue to develop at the lower primary branch. Each PBP, SBP and SP differentiate with differentiation bract or bract hair cell around the base of each their primordia. The observation could confirm that Milyang 23 has not only 2~3 more defferentiated PBPs, but also more SBPs and SPs especially from middle-lower primary branch, at end of their differentiation stages, as compared to those of Koshihikari.

• Journal of Plant Biology
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• v.20 no.3
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• pp.127-134
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• 1977

In order to investigate the changes of free amino acids during the differentiation and development of young spike in naked barley, a typical spring grain, Wanju, and two winter grains, Sedohadaka and Nonsankwa No. 1-6 differing in their spring habits, were analyzed at different growth stages by thin layer chromatography. In all the varieties 22 ninhydrin positive components were detected at the sowing time of March 5 and 20 components in the sowing plots of March 30. In case of the latter plot, β-alanine was identified only in both Wanju and Sedohadaka, whereas pipecolic acid was detected only in Nonsankwa No. 1-6. Particularly, it is interesting that β-alanine was observed only in the case showing the normal heading independent of the varieties and sowing times. Whether these components are directly related to the physiology of spring habits in barley or not is also a question to be answered. Of the major amino acids, alanine and γ-aminobutyric acid were always detected in appreciably large spots, and serine, leucine, aspartic acid, valine and asparagine were somewhat larger. In the plot of march 30, glutamic acid was also detected in very large spot in both Wanju and Sedohadaka at the stage of spikelet differentiation and in Nonsankwa No. 1-6 at the stage of bract differentiation. Histidine, which showed the only qualitative difference among the varieties during seed germination, cannot be observed at all. Proline observed considerably large spot during seed germination was always detected but very small except that it was observed in large spot at the stage of floret differentiation in Nonsankwa No. 1-6 in the plot of March 5.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.31 no.3
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• pp.375-382
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• 1986

This study was carried out to obtain the basic information for the clarification of spring growth habits mechanism of naked barleys. The isozyme patterns and activities of peroxidase in the young spike and leaf blade were analyzed during the differentiation and development of young spike. The characteristic differences between the normal and rosetted type were in c and g isozymes in young spike, and in i isozyme in the leaf blade. In the normal type, c and i isozymes disappeared at the stage of spi-kelet differentiation, g isozyme at the stage of flolet differentiation. But, in the rosetted type, those three isozymes remained in dark stained condition until the time of final sampling. Especially, those three isozymes were higher in the rosetted type than those in the normal type even at the stage of bract differentiation(BDS), just prior to the reproductive stage. The activities of peroxidase decreased slowly after BDS in the young spike and leaf blade in the normal type, While, in the rosetted type, increased linearly, and the degree of increasing was remarkable in the young spike. It was interesting that the degree of activities in young spike was higher in the rosetted type than that in the normal type even at BDS. From the above results, the remarkable differences of the isozyme patterns and activities at BDS between the normal and rosetted type were considered to be the physiological expression of the varieties concerned with the degree of spring growth habits.

• Applied Microscopy
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• v.37 no.2
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• pp.83-91
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• 2007

The present study examined the epidermal changes of adhesive disks which occur during attachment in Parthenocissus tricuspidata using scanning and transmission electron microscopy. Several adhesive disks, each covered with a bract, develop from the shoot apical meristem during early development. In the initial stage, the adhesive disks are club-shaped and their upper and lower epidermis are indistinguishable. However, in the actively growing stage, they become spherical and both epidermis are clearly differentiated into the adventitious roots. Prior to wall attachment, the adhesive disks exhibit adaxial convex and abaxial concave shapes, and electron-dense substances are abundant in the vacuoles of epidermal cells. The peripheral area of the adhesive disk is adhered first to the wall surface, while the central area is drawn inward in a vacuum-like state during attachment. As the attachment progresses and the electron-dense substances continue to discharge, the upper and lower epidermis rapidly undergo deterioration and the disks shrink considerably. At this stage, structural changes of the lower epidermis occur much faster than in the upper one. The discharged substance is accumulated on the wall surface, and this aids the attachment of adhesive disks on the wall for long periods. In this manner, the shape and structure of the adhesive disk epidermis change drastically from initial growth to the mature stage. Further, the role of electron-dense substance and shrinkage of the disk during attachment has been discussed in Parthenocissus tricuspidata.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.24 no.4
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• pp.83-114
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• 1979

These studies were, aimed at clarifying the relationship between the spring(winter) habits and the metabolism during the differentiation and development of young spike in naked barley. The pattern of change of nucleic phosphorus was paralleled to that of insoluble nitrogen in the normal heading type, showing their increase in the young spike and their decrease in the leaf at the stage of double ridges differentiation, respectively. However, in the rosetted type nucleic phosphorus remained at a constantly low level in both the young spike and the leaf, and insoluble nitrogen showed a considerably lower content in the young spike but a remarkable higher content in the leaf than that of the normal type. In addition to nucleic phosphorus and insoluble nitrogen, there were significant differences between the normal and the rosetted type in the content levels of PCA-soluble phosphorus, nonreducing sugar, crude starch and so on. Particularly, these differences were found even in the stage of bract differentiation, the vegetative phase, as well as in the reproductive phase. It appeared that nucleic phosphorus and insoluble nitrogen were closely concerned with the differentiation of double ridges, regardless of the varieties which are different in their spring habits.