• Title/Summary/Keyword: bovine adrenal

Search Result 27, Processing Time 0.023 seconds

Primary Culture of Bovine Capillary Endothelial Cells for In Vitro Angiogenesis Assay

  • Lee, Tae-Hee;Kim, Soung-Soo
    • BMB Reports
    • /
    • v.31 no.2
    • /
    • pp.156-160
    • /
    • 1998
  • In this study, we cultured bovine capillary endothelial cells from adrenal cortex and compared these cells with capillary endothelial cells obtained from bovine adrenal medulla on morphological and cytokinetic properties. We demonstrated that bFGF and gelatin matrix were required for the growth of adrenal cortex-derived capillary endothelial cells over middle passage, but not for the growth of adrenal medulla-derived capillary endothelial cells. Also, we showed that the growth of adrenal cortex-derived capillary endothelial cells must be stimulated by bFGF and the gelatin matrix for the measurement of in vitro angiostatin activity. These data indicate that adrenal cortex-derived capillary endothelial cells over middle passage are more suitable than adrenal medulla-derived capillary endothelial cells for in vitro angiogenesis assay.

  • PDF

Properties of Muscarinic Receptor in Bovine Adrenal Medulla (소 부신수질 Muscarine 수용체의 성질)

  • 이신웅;이해태
    • Biomolecules & Therapeutics
    • /
    • v.2 no.4
    • /
    • pp.361-368
    • /
    • 1994
  • The nature of the muscarinic receptors in bovine adrenal medulla was investigated in this study. [$^3$H]Quinuclidinyl benzilate(QNB) specifically bound to a single class of muscarinic receptor with a $K_{D}$ value of about 70 pM in bovine adrenal medullary, cardiac ventricular and ileal homogenates. Pirenzepine inhibition curves of [$^3$H]QNB binding to cardiac ventricular and ileal homogenates were steep, indicating the presence of a single class of binding site for pirenzepine with a Ki value of 990 nM and 508 nM, respectively. However, pirenzepine/[$^3$H]QNB competition binding curves in adrenal medulla suggested the presence of two binding sites (Hill coefficient=0.59) with a high( $M_1$) and a low( $M_2$) affinity. Respective Ki values for pirenfepine were 16 nM and 633 nM, with 44% of total sites having a high affinity( $M_1$). Gallamine, which is selective to cardiac $M_2$-receptor, inhibited [$^3$H]QNB binding to adrenal medullary, cardiac ventricular and ileal homogenates with Ki values of 12 $\mu$M, 6 $\mu$M and 13 $\mu$M, respectively. Thus, the binding affinities of pirenzepine and gallamine for $M_2$-receptor in adrenal medulla were similar to those in ileum, which contains the $M_3$-receptor. These results indicate that the $M_1$- and $M_3$- muscarinic receptor subtypes coexist in the bovine adrenal medulla.a.

  • PDF

Effect of Ginseng Total Saponin on Bovine Adrenal Tyrosine Hydroxylase

  • Kim, Hack-Seang;Zhang, Yong-He;Fang, Lian-Hua;Lee, Myung-Koo
    • Archives of Pharmacal Research
    • /
    • v.21 no.6
    • /
    • pp.782-784
    • /
    • 1998
  • Ginseng total saponin (GTS) can modulate dopaminergic activity at both presynaptic and postsynaptic dopamine receptors (Kim et al, 1998). The present study investigated t he effect of GTS on the bovine adrenal tyrosine hydroxylase (TH), which catalyze L/tyrosine to DOP. GTS inhibited the bovine adrenal TH by 42.4, 51.5 and 55.3% at concentrations of 40, 80 and 100${\mu}g$/ml, respectively. The IC50 value of GTS was 77.5${\mu}g$/ml. GTS exhibited noncompetitive inhibition with a substrate L-tyrosine. The Ki value was 155${\mu}g$/ml.

  • PDF

Effects of Herbal Drugs on Bovine Adrenal Tyrosine Hydroxylase and Dopamine ${\beta}-Hydroxylase$ (II) (수종의 생약이 Bovine Adrenal Tyrosine Hydroxylase 및 Dopamine ${\beta}-Hydroxylase$ 활성에 미치는 영향 (II))

  • Hwang, Yoon-Jeong;Lee, Seung-Ho;Kim, Hack-Seang;Lee, Kyong-Soon;Ro, Jai-Seup;Lee, Myung-Koo
    • Korean Journal of Pharmacognosy
    • /
    • v.25 no.2
    • /
    • pp.194-197
    • /
    • 1994
  • MeOH extracts of sixteen herbal drugs were tested for the effects on bovine adrenal tyrosine hydroxylase and dopamine ${\beta}-hydroxylase$. The MeOH extracts of Paeoniae Radix and Pinelliae Tuber showed 65% inhibition on the tyrosine hydroxylase activity at the concentration of 100 $\mu$g in the enzyme reaction mixture. Those of Paeoniae Radix, Pinelliae Tuber and Evodiae Fructus showed 87, 84 and 62%, respectively, on the dopamine ${\beta}-hydroxylase$ activity.

  • PDF

Inhibition of Tyrosine Hydroxylase by Palmatine

  • Lee, Myung-Koo;Zhang, Yong-He;Kim, Hack-Seang
    • Archives of Pharmacal Research
    • /
    • v.19 no.4
    • /
    • pp.258-260
    • /
    • 1996
  • Palmatine, an protoberberine isoquinoline alkaloid, has been found to inhibit dopamine biosynthesis by reducing tyrosine hydroxylase (TH) activity in PC12 cells (Lee and Kim, 1996). We have therefore investigated the effects of palmatine on bovine adrenal TH. Palmatine showed a mild inhibition on bovine adrenal TH (36.4% inhibition at concentration of $200\muM$). Bovine adrenal TH was inhibited competitively by palmatine with a substrate L-tyrosine. The Ki value was found to be 0.67 mM. This result suggests that the inhibition of TH activity by palmatine may be partially involved in the reduction of dopamine biosynthesis in PC12 cells.

  • PDF

Inhibitory Effects of Tetrahydropapaveroline on Dopamine Biosynthesis in PC12 Cells (Tetrahydropapaveroline의 PC12 세포내 Dopamine 생합성 저해작용)

  • Lee, Jae-Joon;Kim, Yu-Mi;Kim, Mi-Na;Lee, Myung-Koo
    • YAKHAK HOEJI
    • /
    • v.49 no.2
    • /
    • pp.156-161
    • /
    • 2005
  • Tetrahydropapaveroline (THP) at 5-15 ${\mu}$M has been found to induce L-DOPA-induced oxidative apoptosis in PC12 cells. In this study, the inhibitory effects of THP on dopamine bios ynthesis in PC12 cells and tyrosine hydroxylase (TH) activity in bovine adrenal were investigated. Treatment of PC12 cells with THP at 2.5-10 ${\mu}$M significantly decreased the intracellular dopamine content in a concentration-dependent manner (18.3% inhibition at 10 ${\mu}$M THP). In these conditions, TH activity was markedly inhibited by the treatment with THP at 2.5-10 ${\mu}$M in PC12 cells (23.4% inhibition at 10 $\mu$ M THP). In addition, THP had an inhibitory effect on bovine adrenal TH activity IC50 value, 153.9${\mu}$M). THP exhibited uncompetitive inhibition on bovine adrenal TH activity with a substrate L-tyrosine with the KI value of 0.30 mM. Treatment with L-DOPA at 20~50 ${\mu}$M increased the intracellular dopamine content in PC12 cells, and the increase in dopamine content by L-DOPA was inhibited in part when THP at non-cytotoxic (5-10 ${\mu}$M) or cytotoxic (15${\mu}$M) concentrations was associated with L-DOPA (20 and 50 ${\mu}$M) for 24 h incubation. These results suggest that THP at 5-10${\mu}$M decreases the basal dopamine content and reduces the increased dopamine content induced by L-DOPA in part by the inhibition of TH activity, and that THP at 15${\mu}$M also decreases dopamine content by oxidative stress in PC12 cells.

Effect of Staurosporine on the Long-term Secretion of Catecholamines Induced by Various Secretagogues in Cultured Bovine Adrenal Medullary Chromaffin Cells

  • Choi, Seong-Soo;Suh, Hong-Won
    • The Korean Journal of Physiology and Pharmacology
    • /
    • v.5 no.6
    • /
    • pp.503-510
    • /
    • 2001
  • Long-term treatment of cultured bovine adrenal medullary chromaffin (BAMC) cells with arachidonic acid $(100\;{\mu}M),$ angiotesnin II (100 nM), prostaglandin $E_2\;(PGE_2;\;10\;{\mu}M),$ veratridine $(2\;{\mu}M)$ or KCl (55 mM) for 24 hrs increased both norepinephrine and epinephrine levels in the supernatant. Pretreatment with staurosporine (10 nM), a protein kinase C (PKC) inhibitor, completely blocked increases of norepinephrine and epinephrine secretion induced by arachidonic acid, angiotensin II, $PGE_2,$ veratridine or KCl. In addition, K252a, another PKC inhibitor whose structure is similar to that of staurosporine, effectively attenuated both norepinephrine and epinephrine secretion induced by arachidonic acid. However, K252a did not affect the catecholamine secretion induced by angiotensin II, $PGE_2,$ veratridine or KCl. Our results suggest that staurosporine may inhibit long-term catecholamine secretion induced by various secretagogues in a mechanism other than inhibiting PKC signaling. Furthermore, long-term secretion of catecholamines induced by arachidonic acid may be dependent on PKC pathway.

  • PDF

Mechanism of leptin-induced catecholamine secretion in the perfused rat adrenal medulla

  • Lim, Dong-Yoon;Kim, Ok-Min;Shin, Hye-Gyeong
    • Proceedings of the PSK Conference
    • /
    • 2003.10b
    • /
    • pp.83.2-83.2
    • /
    • 2003
  • It has been demonstrated the presence of leptin receptors (Ob-Ra) on epinephrine-secreting chromaffin cells in rat adrenal medulla, suggesting that leptin may directly affect the adrenal medulla (Cao et al., 1997). Leptin is found to stimulate catecholamine (CA) synthesis in cultured bovine adrenal medullary cells (Utsumomiya et al., 2001; Shibuya et al., 2002)and cultured porcine adrenal medullary cells (Takekoshi et al., 2001). Thus, the present study was designed to examine the effect of leptin on CA release from the isolated perfused rat adrenal gland, and to establish its mechanism of action. (omitted)

  • PDF

REGULATION OF PROENKEPHALIN GENE EXPRESSION AND MET-ENKEPHALIN SECRETION IN BOVINE ADRENAL MEDULLARY CHROMAFFIN CELLS AND C6 RAT GLIOMA CELLS

  • Suh, Hong-Won
    • Toxicological Research
    • /
    • v.9 no.2
    • /
    • pp.195-206
    • /
    • 1993
  • The expression of proenkephalin (proENK) mRNA and Met-enkephalin (ME) secretion in C6 rat glioma cells and bovine adrenal medullary chromaffin (BAMC) cells were elucidated in the present study. The levels of proENK mRNA and ME secreted into the media in BAMC cells were measured in the presence of cycloheximide and 12-tetrade-canoylphorbol-13-acetate (TPA). Cycloheximide (20 nM) abolished the induction of proENK mRNA expression, protein synthesis and ME secretion by TPA (1nM), indicating that de novo protein synthesis was necessary for proENK gene expression and ME secretion.

  • PDF

Dopamine ${\beta}-Hydroxylase$ Inhibitory Activity of Medicinal Plants (식물성 생약의 도파민 베타 수산화효소에 대한 저해활성)

  • Tae, Dong-Nyen;Hwang, Keum-Hee;Han, Yong-Nam
    • Korean Journal of Pharmacognosy
    • /
    • v.26 no.1
    • /
    • pp.62-65
    • /
    • 1995
  • Dopamine ${\beta}-hydroxylase$ (DBH) catalyses the enzymatic reaction of dopamine to norepinephrine. For the purpose of isolating DBH inhibitors from natural resources, thirty one kinds of medicinal plants were screened by tracing the inhibitory activities against bovine adrenal DBH, utilizing tyramine as a substrate. Among the crude drugs tested, leaves of Lactuca sativa L., Gardeniae Fructus, Magnoliae Flos and Scutellariae Radix showed potent enzyme inhibitory activities against DBH.

  • PDF