• Korean Institute of Interior Design Journal
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• no.14
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• pp.82-89
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• 1998

During the boom of museum building in the 1970s-80s two museum wee erected in Germany. They are James Stirling's Neue Stattsgalerie Stuttgart and hans Hollein's Stadtisches Museum Abteiberg Monchengladbach, These two museums share the folowing point of similarity : Stirling's Neue Stattsgalerie Stuttgart and Hollein's Staditisches Museum Abetiberg Monchenbladbach both manifest the conditions of the times in light of their respective cities' local characters and historical contexts without being bound to traditional formality. Stirling and Hollein attempted to grasp the meaning of the 기nuseum as a city in miniature." Taking into account the t two museums’s territorial characteristics of being situated on a slope, both Stirling and Hollein made the walker to pass t through their building complex and introduced the concept of a public square within them. As a result, the museums are not j just two large buildings but are architecture composed of a collage of various structures. S Stirling’s architecture employs the method of attaching additional elements on top of basic constituents, which is suggestive '||'&'||'#61551; of the historical fragment from Shinkel's Altes Museum. On the other hand, Hollein applies a collage-style method as if he w were doing urban planning, maintaining the distinctiveness of each of the various forms and materials of buildings. T The object style buildings of the two museums actively demonstrate the contrast of double meaning to represent the a ambiguous and multifarious characteristics of the modern times. Stirling explores the theme of opposition and coexistence in h his Neue Stattsgalerie Stuttgart by placing a series of opposing concepts, such as the past and present and histor${\gamma}$ and t technology, in one space. Thereby, the contradiction and its appeal are manifested. Hollein made use of the visual illusion and c contradiction in alluding to the irony of the modern reality induced by nature and culture and history and technology. F For the above reasons and methods, James Stirling ’ s Neue Stattsgalerie Stuttgart allowed the penetration of art into daily l life and became the general public’s favored museum for its free and unrestrained environment. Likewise, Hans Hollein’s S Stadtisches Museum Abteiberg Moncbengladbach contribute to the development of museum function as it an expression of art i in the form of a complex piece of scupture on its own.ts own.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.2
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• pp.683-688
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• 2018

In this study, we analyzed the heat generation state of a flat heating element by using image processing technology in conjunction with carbon fiber. The flat heating element is manufactured by chopping the carbon fiber to a small size and bonding it again using a dispersing agent. The solution of carbon fiber, bound together using the dispersant, is then filtered onto the nonwoven fabric. The last step is to obtain flat carbon fibers in the form of nonwoven fabrics for the purpose of drying the filtered carbon fibers. In the flat heating element, electricity may be applied to the carbon fiber on the surface produced in this manner. In this study, the flat heating element was analyzed by four methods. The analysis of the heat generation characteristics and heating rate of the flat heating element confirmed that the fabricated sheet heating element corresponds to a normal army. The analysis of the insulation coating and flat heating element module, which can be used for actual product manufacturing, involves two dimensional image analysis using image processing technology. The thermal image analysis of the flat heating element is a programming technique that not only analyzes the heat generation state in both two and three dimensions, but also displays the upper and lower 15 to 20% ranges of temperature corresponding to the heat generation in the image. In the final analysis, it is possible to easily find the erroneous part in the manufacturing process by directly showing the state of the fabricated flat heating element on the screen. By combining this image analysis method of the flat heating element with the existing method, we were able to more accurately analyze the heat generation state.

• Korean Journal of Ecology and Environment
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• v.33 no.4 s.92
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• pp.380-386
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• 2000

Alkaline phosphatase ($AP_{ase}$) activity was studied for the pure cultures and natural communities of phytoplankton. The Michaelis-Menten constant ($K_m$) showed large variation with species. Some green algae showed large $K_m$ ($650\;{\mu}M$ for Selenastrum capriconutum). Chlorella sp. and Nitzschia palea showed smaller Km (respectively1.7, 2.0 HM) than those of other species examined. The extracellular free enzyme in the filtrate of Anabaena flos-aquae showed smaller Km ($52\;{\mu}M$) than that of cellbound form ($276\;{\mu}M$). The $K_m$ ($12.0\;{\mu}M$) of summer phytoplankton in Lake Soyang. when Anabaena sp. was dominant species, was larger than that (1.5 HM) of spring phytoplankton when Asterionella sp. was the dominant. Although maximum activity($V_{max}$) in Lake Soyang was affected by the concentration of DIP within the lake, but the $V_{max}$ always varied not with the DIP concentration of the lake. Induction of $AP_{ase}$ may be more affected by the phosphate content within the cell of phytoplankton than by the concentration of DIP within the lake, The extracellular free $AP_{ase}$ activity accounted for $36{\sim}97%$ of total activity from fall to spring turnover in Lake Soyang. The $K_m$ ($1.1{\mu}3.5\;M$) of extracelluar free enzyme were simillar to those ($0.7{\mu}3.5\;M$) of the total activity. This indicates that the extracelluar free enzyme was derived from phytoplankton.

• Journal of Korea Multimedia Society
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• v.9 no.9
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• pp.1118-1130
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• 2006

Existed pattern analysis algorithms in data streams environment have researched performance improvement and effective memory usage. But when new data streams come, existed pattern analysis algorithms have to analyze patterns again and have to generate pattern tree again. This approach needs many calculations in real situation that needs real time pattern analysis. This paper proposes a method that continuously analyzes patterns of incoming data streams in real time. This method analyzes patterns fast, and thereafter obtains real time patterns by updating previously analyzed patterns. The incoming data streams are divided into several sequences based on time based window. Informations of the sequences are inputted into a hash table. When the number of the sequences are over predefined bound, patterns are analyzed from the hash table. The patterns form a pattern tree, and later created new patterns update the pattern tree. In this way, real time patterns are always maintained in the pattern tree. During pattern analysis, suffixes of both new pattern and existed pattern in the tree can be same. Then a pointer is created from the new pattern to the existed pattern. This method reduce calculation time during duplicated pattern analysis. And old patterns in the tree are deleted easily by FIFO method. The advantage of our algorithm is proved by performance comparison with existed method, MILE, in a condition that pattern is changed continuously. And we look around performance variation by changing several variable in the algorithm.

• Korean Journal of Microbiology
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• v.31 no.4
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• pp.279-285
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• 1993

Alterations of the p53 gene arc among the most frequent genetic changes in human cancer and often result in increased levels of p53 protein within the malignant cells. Detection of accumulated p53 protein can be a useful prognostic tool in human cancer. In order to make polyclonal antibodies for immunohistochemical screening. human p53 gene was expressed in E. coli in the form of GST (glutathione S-transfi.:rase) fusion proteins. Two p53 gene fragments. which were N('()I small fragment encoding amino acid residues of 1-151-: and Ncol large fragment of 159-393. were subeloned into the unique BamHI site present within the pGEX-2T vector using BamHI linker and recombinant plasmids pGTNS and pGTNL were constructed. respectively. The p53 cDNA fragment (from pC53-$SN_3$,) encoding amino acid 38-145 (proline at residue 72) was amplified by polymerase chain reaction(PCR). The amplified DNA was digested with BamHI and Prull and inserted into the BamHI-Smal sites of pG EX-2T and recombinant plasmid pGTBP was constructed. After IPTG induction of these plasmids for 4 hours. fusion proteins were purified from E. coli extracts with glutathione Sepharose beads. The bound proteins were resolved by 10% SDS-polyacrylamide gel electrophoresis and the molecular weights were 54 kDa. 53 kDa and 40 kDa. respectively. Approximately one milligram of fusion proteins were purified from 1 -liter cultures.

• Korean Journal of Environmental Agriculture
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• v.5 no.1
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• pp.30-34
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• 1986

This study was carried out to determine the heavy metal contents in sluges of Keumho river and her branches. The heavy metal contents of sludge are the highest in Sincheon, Kongdancheon and Dalseocheon among the branches. The large part of heavy metal exists in $0{\sim}5cm$ from sludge surface. The sequential extraction with various reagents showed that the residual and organically bound fraction were the most abundant pool. Decomposition of organic matter caused sludge to release heavy metals. On the extraction of sludge with various solution having different pH, it was found that the lower the pH, the more heavy metal was extracted. However, considerable amount of heavy metal was not extracted even with pH 3 solution.

• Applied Microscopy
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• v.41 no.4
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• pp.229-234
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• 2011

The occurrence of nuclear inclusions has been reported in various plant groups from primitive ferns to higher flowering plants. Their presence within a group seems to be randomly distributed without any phylogenetic relationships among species. According to the current survey, nuclear inclusions have been widely documented in more than several hundreds of species from various families of plants. The morphology and internal structures of nuclear inclusions are diverse and at least five types of inclusions develop within plant nuclei; amorphous, crystalline, fibrous, lamellar, and tubular form. Among these types, crystalline inclusions are the ones that are the most frequently reported. The inclusions are not bound by membranes and appear to be related to the nucleoli, either spatially by a close association or by an inverse relationship in size during development. The idea that nuclear inclusions are of a proteinaceous nature has been widely accepted. Further link to nucleolar activity as a protein storing site has also been suggested based on the association between the nucleolus and nuclear inclusions. Various investigations of nuclear inclusions have revealed more information about their structural features, but characterizing their precise function and subunit complexity employing molecular analysis and 3-D reconstruction remains to be elucidated. Tilting and tomography of serial sections with appropriate image processing can provide valuable information on their subunit(s). The present review summarizes discussion about different nuclear inclusions in plants from previous works, giving special attention to their fine, ultrastructural morphology, function, and origin.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.33 no.5
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• pp.403-407
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• 2000

Ultrastructure and function of testis somatic cells in freshwater prawns Macrobrachium nipponense were studied. The paired testes of the prawn were elongated, united at their anterior end, which lay between the dorsal surface of the hepatopancreas and the heart. Each testis consisted of a large number of seminiferous cords compactly held together by connective tissue. A seminiferous cord was composed of an outer layer of simple squamous epithelium, a basement membrane, the closely packed germ cells and sustentacular cells of the germinal ridge, and an inner layer of simple cuboidal epithelial cells. Leydig cell-like cells in an angular areas filling the space of the seminiferous cords were observed. The nuclei of leydig cell-like cells were characterized by a distinct nucleolus. The simple squamous epithelial layer was composed of flattened cells tying on a basement membrane. The nuclei of the flattened cells were often overlapped in a layer, and the cytoplasm of the cells was observed just near the nuclei. The sustentacular cells were complex in morphology. These cells had relatively small cell bodies from which long cytoplasmic extensions ramified reached the space of germ cells in the germinal ridge. The nuclei of sustentacular cells usually exhibited angular profiles and located most commonly at the periphery of the cords. Cells of simple cuboidal epithelium located between germinal ridge and lumen of seminiferous cord, and part of the cells were adjacent to basal lamina, The cuboidal epithelial cells contained numerous mitochondria, the well-developed rER, the well-developed Golgi complex, and irregularly shaped nuclei. Transition vesicles appeared on the cis side of the Golgi complex. The large vesicles on the trans side of the complex appeared to fuse to form a membrane-bound structure. A number of pits on the cell apex suggested exocytotic activity for secretion of the sperm supporting matrix.

• The Korean Journal of Pesticide Science
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• v.3 no.2
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• pp.54-59
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• 1999

In order to investigate the behavior of the herbicide metolachlor [2-chloro-6'-ethyl-N-(2-methoxy-1-methylethyl)aceto-O-toluidide] in fish, carps (Cyprinus carpio L.) were exposed to the herbicide at $LC_{10}$(1.93 mg/L) for 4 days. Metolachlor dissolved in water was absorbed rapidly into carps to mark the maximum concentration 6 hours after exposure. The amounts of the $^{14}C$-metolachlor and its metabolites absorbed in gall were much higher than those in the other parts, strongly suggesting that biliary excretion involving enterohepatic recirculation could be an important route for the elimination of metolachlor. The $^{14}C$-radioactivity distributed into aqueous phase fraction in test water and in carp extract was increased in time-dependent manner. Extraction rate of $^{14}C$ absorbed in carp tissues was decreased remarkably up to 6 hours after exposure, suggesting that the possible polar metabolites of metolachlor were transformed into the conjugates to form non-extractable bound residues.

• The Journal of the Acoustical Society of Korea
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• v.35 no.6
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• pp.473-479
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• 2016

We report a highly selective and sensitive 200 MHz Surface Acoustic Wave (SAW) sensor that can detect cyanide ion in aqueous solution using surface immobilized thioester molecules in combination with gold nanoparticles (AuNPs). To construct the sensor device, a monolayer of thioester compound was immobilized on the SAW sensor surface. At the sensor surface, hydrolysis of thioester group by nucleophilic addition of cyanide occurred and the resulting free thiol unit bound to AuNP to form thiol-AuNP conjugate. For the signal enhancement, gold staining signal amplification process was introduced subsequently with gold (III) chloride trihydrate and reducing agent, hydroxylamine hydrochloride. The SAW sensor showed a detection ability of $17.7{\mu}M$ for cyanide in aqueous solution and demonstrated a saturation behavior between the frequency shift and the concentration of cyanide ion. On the other hand, our SAW sensor had no activities for other anions such as fluoride ion, acetate ion and sulfate ion, moreover, no significant interference observed by other anions. Finally, all the experiments were carried out in-house developed sensor and fluidics modules to obtain highly reproducible results.