• Title/Summary/Keyword: bottle culture

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Bottle Cultivation of Pleurotus ostreatus, Agrocybe aegerita and Ganoderma lucidum using Rice hull media

  • Lee, he-duck;Kim, hong-kyu;Kim, yong-gyun;Lee, ga-soon
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2001.04a
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    • pp.44-46
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    • 2001
  • Rice hull was used as a additive in order to find the effect for incresing of mushroom growth and yield in Chungnam Provincial techinical institution. 1 Treatment of 80% rice hull in small Neutaribeosut mycelial grow duration is shorter about 11 days and yield increased about 7% than conventional culture. 2. In case of Chongpung Neutaribeosut bottle culture, mycelial growth duration is shorter about two to three days in additive of 30 to 80% rice hull compared to conventional but yield similar to conventional. 3. Treatment of 30% rice hull in Agrocybe aegerita bottle culture, mycelial growth and yield increased 6days and 6% than convrntional, respectively 4 Treatment additived of 30% to 40% rice hull in Ganoderma lucidum bottle culture, similar to 454ays demand in mycelial grow duration and 38g yield/bottle in conventioal culture methods.

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Studies on the development of mushroom media for bottle culture in new Pleurotus ostreatus 'Miso' (신품종 '미소' 느타리버섯 병재배 배지개발)

  • Lee, Byung-Joo;Kim, Yong-Gyun;Kim, Hong-Kyu;Yang, Euy-Seog;Lim, Yong-Pyo
    • Journal of Mushroom
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    • v.8 no.1
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    • pp.37-40
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    • 2010
  • This study was carried out to develop the best medium of new oyster mushroom for bottle culture. The new oyster mushroom cv. Miso is a Pleurotus ostreatus developed at the Chungnam Agricultural Research & Extension Services. For the bottle culture media, poplar sawdust+beet pulp+cottonseed meal (5:3:2), poplar sawdust+wheat husk meal (8:2), poplar sawdust+beet pulp+cottonseed meal (6:2:2), poplar sawdust+beet pulp+cottonseed meal (4:4:2), and poplar sawdust+beet pulp+wheat husk meal (7:1:2) were used in 850cc PP bottle. The pH was 5~6 and the C/N ratio 19.7~28.3 in bottle culture media. The time of pinhead formation was 5 to 6 days. For the fruiting body formation after inoculation took 29~31 days. The yield of fruiting body of poplar sawdust+beet pulp+wheat husk meal (7:1:2) medium was the highest at 110.4g/bottle compared to other media. Therefore, such cultivation medium would be appropriate for the commercial production of bottle culture in the new oyster mushroom 'Miso'.

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Automatic Feeding Bottle Washing Machine Usability Evaluation : Infant Product (자동 젖병 세척기 사용성평가 : 영유아 제품)

  • Park, Hye Jin;Kim, Jong Geun;Lee, Seok Jae
    • The Journal of the Convergence on Culture Technology
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    • v.8 no.3
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    • pp.507-514
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    • 2022
  • This study is to compare and analyze the evaluation of cleaning cleanliness and usability according to the muscle activity and cleaning method in the wrist rotation motion when using a bottle washer for women with childbirth experience. To this end, when using an automatic bottle washer and a manual bottle washer, the muscle activity and cleaning cleanliness of the forearm muscles were quantitatively evaluated using equipment, and the effects of variables were analyzed and the following conclusions were obtained. First, it was found that the muscle activity was lower than that of the automatic baby bottle cleaner when using a manual bottle cleaner. Second, the cleanliness was judged to be the best in automatic washing (forward + reverse direction), followed by automatic washing (forward direction) and manual washing. Third, the usability evaluation of the use of the baby bottle washing machine showed higher satisfaction than that of the automatic baby bottle washing machine. Through these conclusions, this study can be used as basic data for product advancement in the future by proving the effectiveness of using an automatic bottle washer.

Bottle Cultivation of Pleurotus ostreatus, Agrocybe aegerita and Ganoderma lucidum by using Rice hull media (왕겨배지를 이용한 느타리, 버들송이, 영지 병재배 효과)

  • 이희덕;김홍규;김용균;이가순
    • Korean Journal of Plant Resources
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    • v.14 no.3
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    • pp.213-219
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    • 2001
  • Rice hull was used as an additive in order to determine the its effect on increse of mushroom growth and yield in Chungnam Provincial techinical institution. In the treatment of 80% rice hull in small Neutaribeosut, mycelial growth duration was shown to be shorter about 11 days and yield increased about 7oie than those of conventional culture. In the case of Chongpung Neutaribeosut bottle culture, mycelial growth duration was shorter about 2 to 3 days in additive of 30 to 80% rice hull compared to conventional, but yield was similar to that of conventional. In the treatment of 30% rice hull in Agrocybe aegerita bottle culture, mycelial growth and yield increased 6 days and 6% than those of convrntional, respectively. In the treatment of 30% to 40% rice hull in Ganoderma lucidum bottle culture, mycelial grow durations were similar 45 days and 38 g/bottle.

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Studies on the aeration improvement of inner bottle(850ml) culture system during the mycelial culture of Pleurotus ostreatus (느타리의 균사 배양 중 배양기 내부 통기성 개선)

  • Yoo, Young-Jin;Shim, Kyu-Kwang;Koo, Chang-Duck;Kim, Myung-Koon
    • Journal of Mushroom
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    • v.10 no.2
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    • pp.57-62
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    • 2012
  • The plastic culture bottle cap types and accumulated concentration of carbon dioxide, media humidity in the process of medium culture, chitin content and yield were observed in Pleurotus ostreatus 850ml bottle in Iksan, Jeollabuk-do, Korea, during 2011. The concentration of carbon dioxide in the process of medium culture was the highest after 8~9 days cultivation irrespective of cap sizes and types. The accumulated concentration of carbon dioxide in size cap of 29~41mm was 6.5~4.0% in the upper-under perforation hole of cap and 9.0~6.5% in the under perforation hole of cap. The upper-under 23~33mm perforation hole and under 29mm perforation hole of caps in the 850ml bottle were best condition for cultivation of mushroom and increased fruit body, 15.8~21.2% and 20%, respectively. However, the upper-under & under 41mm perforation hole of fruit body were decreased 60.7% and 23.6%, respectively. Also it was weak, lose vitality and the lower of biologically activity substance because the upper medium humidity was too dry.

Studies on the aeration improvement of inner bottle(1100ml) culture system during the mycelial culture of Pleurotus ostreatus (느타리(1100㎖병)의 균사 배양 중 배양기 내부 통기성 개선)

  • Yoo, Young-Jin;Shim, Kyu-Kwang;Koo, Chang-Duck;Kim, Myung-Koon
    • Journal of Mushroom
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    • v.10 no.2
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    • pp.68-73
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    • 2012
  • The plastic culture bottle cap types and accumulated concentration of carbon dioxide, media humidity in the process of medium culture and yield were observed in Pleurotus ostreatus 1,100ml bottle in Iksan, Jeollabuk-do, Korea, during 2011. The concentration of carbon dioxide in the process of medium culture was the highest after 6~11days cultivation irrespective of cap sizes and types. The upper-under 19~38mm perforation hole and under 26~47mm perforation hole of caps in the 1,100ml bottle were best condition for cultivation of mushroom and increased fruit body, 11.4~23.8% and 6.5~17.9%, respectively. However, the upper-under 33mm perforation hole of fruit body were decreased 23.8%. Also it was weak, lose vitality and the lower of biologically activity substance because the upper medium humidity was too dry.

A study on the influence of Chinese traditional 'JUGI(酒具)' culture on the modern bottle design (중국 고대 주기(酒具) 문화가 현대주기 디자인에 미치는 영향에 관한 연구)

  • Zhang, Xuejiao;Lee, Dong Hun
    • Proceedings of the Korea Contents Association Conference
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    • 2008.05a
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    • pp.681-684
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    • 2008
  • To the 'JUGI(酒具)' culture in ancient China on the basis of the conclusions reached, the development of modern bottle design with the status quo for Chinese wine with the design of innovative and local Chinese-style design ideas.

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Studies on Improvement of Cultural Practice for Lyopyllum ulmarium (만가닥버섯 병재배법 개선 연구)

  • Chi, Jeong-Hyun;Park, Woo-Kill;Kim, Young-Ho
    • The Korean Journal of Mycology
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    • v.28 no.2
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    • pp.88-92
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    • 2000
  • Substrate improvement, maturation period and method of pinheading promotion were investigated to establish the culture method of Lyopyllum ulmarium which could obtained high yield in the short culture period. Rice bran+wheat bran (10%+10%) combination was selected from various additives, which showed vigorous growth of fruit body and high yield (133.8 g/850 cc). Oyster shells powder substance increased yield to 155.5 g per 850 cc capacity bottle. The suitable method for pinheading promotion was to stand the bottle reversely. The number of fruit bodies per 850 cc capacity bottle in this method was 27.2 stipes and bunch formation was good. When the maturation period of mycelium culture was 15 days to 30 days, pinheading period was 10 days. But when the maturing mycelium was 30 to 45 days, yield per 850 cc bottle was 148.2 g.

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Growth Characteristics of an Attenuated Japanese Encephalitis Virus in a Monkey Kidney Cell (Vero) (베로 세포에 적응된 약독화 일본뇌염바이러스의 성장 특성)

  • 홍선표;정용주;문상범;신영철;이성희;김수옥
    • KSBB Journal
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    • v.13 no.3
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    • pp.231-237
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    • 1998
  • An attenuated Japanese encephalitis virus (JEV) clone SA-14-14-2(Vero) was obtained through successive adaptation of a primary cell adapted strain, SA-14-14-2(PDK) to Vero cell, a continuous line of monkey kidney cells. The virus titer reached above the 107 plaque forming unit (pfu) per mL of culture supernatant with 3 passages in Vero cells and was maintained close to this level in the further passages. The optimum temperature for the virus growth was $35^{\circ}C$. Growth pattern of the virus indicated that optimum time for the virus harvest is 4 days post infection and the virus accomplished rapid initial propagation even in medium containing no serum supplement. The roller bottle (RB) system and the spinner flask (SF) system using micro-carrier (Cytodex-1) for the JEV cultivation were explored. When RB, SF, and T-flask culture system were compared, there was no significant difference in virus yield. Furthermore, the results indicated that virus could be harvested multiple times from 3 days to 9 days post infection; neither severe cytopathic effect (CPE) in the infected cells nor the decrease in the titer was observed on duration of 9 days.

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Diversity and origin of bottle gourd, Lagenaria

  • Yuasa, Hiroshi
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2002.11a
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    • pp.96-97
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    • 2002
  • Bottle gourd, Lagenaria siceraria, is one of the oldest cultivated plants. To bigin with, its fruit was used as a complete liquid bottle or container. It was a very widespread cultivated plant in prehistoric times, for example (there) is a report from Peru as early as between 13,000 B.C and 11,000 B.C. The dug-out finds in Japan proved to be about 95,000 years old according to the /sup 14/C analysis. The bottle grourd was the most important plant before the invention of pottery in many areas of Asia, New Guinea, Polynesia, America, and Africa. I would like to suggest that there should be "The Bottle Gourd Age" prior to the Pottery Age. Bottle gourds are also used for various purposes such as food, masks, pipes, musical instruments, medicine, symbols, artistic products and also as penis-sheaths of men's attire. Their purposes number more than 220 including 70 varienties of containers or bottles. I consider that its utilization should be called a culture, as it were, "The gourd culture." The shape and the size of the fruit of bottle gourd have a larger variety than those of any other plant. As for the size, it is reported that they range from those that are shorter than 3cm to those that are ovoid and longer than 60cm in diameter. With regard to the shape of its fruit, the bottle gourd can be classified into 7 groups and even more than 30 races, considering the difference of the size. The seeds are so variable without two horn-like projections, with smooth surface or longitudinalines, white or brown, with smooth corky margin. Generally, it seems that there is no correlation between seem shape and fruit shape. My study shows that the seeds of gourd. My study shows that the seeds of gourd in Asia are so simple in shape and in color except for the size. But the seeds of those in Africa are various and seem to be beyond the confines. Explaining the great diversity of the seeds of those in Africa therefore, they appear to have no correlation among the types of fruit of the bottle gourd with African origin. It might be supposed that another 4 wild Lagenaria spp. distribute only in Africa. The intraspecies hybrid is confirmed between the bottle gourd, Lagenaria siceraria, and wild L. spehaerica. And Fl hybrid is sterile: it has no fertility. However, even though the shapes of the fruit and the seeds are different, they can cross with each other. Moreover, their hybrids grow up to be extremely vigorous and have normal fertility. Fruit shapes of Fl plants are similar to those of their parents, if they have the same character. Whereas, the fruit shape often became different from each other when the cross occurs between those with different fruit shapes. It is shown that dumbbell shapes, HYOTAN in Japanese, is recessive while its bitterness is dominant. Thus the character of F2 hybrids Is segregative. I'll give further particulars of the heredity of the groud in my lecture.

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