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Production and Properties of Hemicellulases by an Isolate of Microbacterium sp. (Microbacterium sp. 분리균의 Hemicellulases 생산성과 효소특성)

  • Yoon, Ki-Hong
    • Korean Journal of Microbiology
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    • v.47 no.3
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    • pp.225-230
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    • 2011
  • A bacterium producing the extracellular mannanase and xylanase was isolated from Korean farm soil by successive subcultures in a minimal medium supplemented with palm kernel meal (PKM) and rice bran. The isolate YB-1106 showed 98% similarity with Microbacterium arabinogalactanolyticum on the basis of 16S rRNA gene sequences. The additional carbohydrates including locust bean gum (LBG) and PKM increased the mannanase productivity of the YB-1106, while the xylanase productivity of the isolate was increased by wheat bran, oat spelt xylan, rice bran and xylose. Particularly, maximum mannanase and xylanase activities were obtained in the culture filtrate of tryptic soy broth supplemented with 1% LBG or 2% wheat bran, respectively. Both enzyme activities were produced at stationary growth phase. The mannanase of culture supernatant was the most active at $50^{\circ}C$ and pH 6.0, while xylanase of culture supernatant was the most active at $55^{\circ}C$ and pH 6.5. The predominant products resulting from the mannanase or xylanase hydrolysis were oligosaccharides for LBG or xylan, respectively.

Full Thickness Skin Graft Using Palmar Crease (손바닥피부주름을 이용한 전층피부이식술)

  • Choi, Yo-Ahn;Choi, Hwan-Jun;Kim, Jun-Hyuk;Lee, Young-Man
    • Archives of Plastic Surgery
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    • v.38 no.6
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    • pp.829-835
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    • 2011
  • Purpose: The two major concerns in skin grafting are poor color match in the recipient site and the donor site morbidity. And, glabrous skin on the palmar aspect of the hands and plantar aspect of the feet attributes define the skin on the palm and fingers sole as functionally and aesthetically different from skin on other parts of the body. When there is a glabrous skin defect, it should be replaced with similar skin to restore function and aesthetics. The palmar crease areas were used to minimize these problems. The purpose of this study is to present the precise surgical technique of the full thickness skin graft using distal palmar and midpalmar creases for aesthetic better outcome for hand injuries. Methods: From May 2006 to April 2010, 10 patients with 11 defects underwent glabrous full thickness skin grafting of finger defects. Causes included seven machinery injuries, two secondary burn reconstructions, and one knife injury. Donor sites included ten glabrous full thickness skin graft from the distal palmar crease and one from the midpalmar crease. Results: Follow-up ranged from 3 months to 24 months. All glabrous skin grafts demonstrated complete taking the recipient sites and no incidence of the complete or partial loss. The donor site healed without complications, and there were no incidences of significant hypopigmantation, hyperpigmentation, or hypertrophic scarring. Conclusion: The important aspects of this method involve immediate return of glabrous skin to the defect site and restoration of the recipient site's crease by simple primary closure from adjacent skin. The glabrous skin of the palm provides the best tissue match for the reconstruction of the hands, but only a limited amount of tissue is available for this purpose. Full thickness skin grafting using palmar crease of the defects is the ideal way of reconstructing glabrous skin to restore both function and aesthetics and minimize donor site morbidity.

Distribution of Skin Hydration on the Hand while Wearing Latex Gloves and Inner Gloves (라텍스 장갑과 마이크로 파이버 속장갑 착용 시 손의 피부수분도 분포)

  • Roh, Sang-Hyun;Hyun, Cheol-Seung;Lee, Joo-Young
    • Journal of the Korean Society of Clothing and Textiles
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    • v.41 no.5
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    • pp.966-976
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    • 2017
  • This study investigated the effects of wearing latex gloves with inner gloves on the skin hydration of the hands. Fifteen young males participated in the following three conditions: bare hand (BH), latex glove (LG), and latex glove with inner glove condition (LGIG) at an air temperature of $28^{\circ}C$ with 50%RH. Subjects typed a book for 120 min. The results were as follows. Skin hydration was greater for LG and LGIG than BH (p<.001), but no difference was found between LG and LGIG. Skin hydration showed greater values on the thenar and dorsum compared to the palm for both LG and LGIG (p<.05). Skin hydration on the thenar increased during the typing for LG and LGIG, but on the dorsum, palm and finger maintained after 40 min typing. There were positive relationships between hand skin hydration and hand temperature (p<.05). Subjects indicated 'much warmer' and 'more humid' for the LG and LGIG compared to the BH (p<.001), but no differences were found between LG and LGIG. In conclusion, wearing inner gloves inside latex gloves did not induce a reduction of skin hydration and hand temperature; however, significant differences were found in all measurements between bare hands and gloved hands.

Classification of Hand Types for the Development of Glove Patterns (장갑 패턴 개발을 위한 손의 유형 분류)

  • Lim, Ji-Young
    • Journal of the Korean Home Economics Association
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    • v.43 no.8 s.210
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    • pp.115-122
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    • 2005
  • The hand performs functions such as grabbing and other movements. In order to accomplish these movements in various kinds of operational environments, appropriate gloves must be worn to protect your hands. Choosing the appropriate type of glove is very important when wearing gloves in these types of operational environments. The reasons one wears gloves varies depending on age and gender. Unmarried women in their early twenties, for example, occasionally wear gloves for decoration rather than for functional reasons. However, previous studies examined a range of topics, and as such investigations dealing with specific body shape and demands of consumer are needed. Therefore in this study, the hands of unmarried women ranging in age from 20 to 24 were measured and hand shape types were analyzed in order to present basal data which can be used to help design improved glove patterns and produce appropriate, functional gloves. A total of 261 Korean women were measured. Fifty-seven right hand dimensions were measured and five dimensions from both hands were measured. Six factors were identified through factor analysis and those factors constituted $73.259\%$ of total variance. Two clusters of hand shapes were categorized using 6 factor scores by cluster analysis. Type 1 hand shape is defined as long hands with small width, girth, and thickness, long and thin fingers, and high vertical palm height. Type 2 hand shape is defined as short hands with large width, girth and thickness, short fingers, thick knuckles, and short vertical palm height. The characteristics of type 1 and 2 hand shapes are similar to women's hand type classification results from previous reports, but there was a significant difference in subject distribution by type. Therefore, standard data on hand shapes should be produced by developing measuring instruments and selling more accurate standard measuring points. By doing this it could help in the development of improved glove patterns, and also aid in planning production based on hand type.

Production and Properties of Hemicellulases by a Cellulosimicrobium sp. Isolate (Cellulosimicrobium sp. 분리균의 Hemicellulases 생산성과 효소특성)

  • Yoon, Ki-Hong
    • Microbiology and Biotechnology Letters
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    • v.39 no.3
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    • pp.252-258
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    • 2011
  • A bacterial strain capable of hydrolyzing xylan and locust bean gum (LBG) was isolated from farm soil by enrichment culture using mixture of palm kernel meal (PKM) and wheat bran as carbon source. Nucleotide sequence of 16S rDNA amplified from the isolate YB-1107 showed high similarity with those of genus Cellulosimicrobium strains. Xylanase productivity was increased when the Cellulosimicrobium sp. YB-1107 was grown in the presence of wheat bran or oat spelt xylan, while mannanase productivity was increased drastically when grown in the presence of PKM or LBG. Particularly, maximum mannanase and xylanase activities were obtained in the culture filtrate of media containing 0.7% PKM or 1% wheat bran, respectively. Both enzyme activities were produced at stationary growth phase. Mannanase from the culture filtrate showed the highest activity at $55^{\circ}C$ and pH 6.5. Xylanase activity was optimal at $65^{\circ}C$ and pH 5.5. The predominant products resulting from the mannanase or xylanase hydrolysis were oligosaccharides for LBG or xylan, respectively. In addition, the enzymes could hydrolyze wheat bran and rice bran into oligosaccharides.

Wear Comfort of Firefighters Protective Gloves in Dry and Wet Conditions at 70℃ Air Temperature with Radiant Heat (기온 70℃의 복사열 노출 환경에서 건조와 젖은 상태의 소방용 보호장갑 착용 시 쾌적성 평가)

  • Kim, Dami;Kim, Dohyung;Lee, Joo-Young
    • Journal of Korean Living Environment System
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    • v.24 no.1
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    • pp.95-106
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    • 2017
  • The purpose of this study was to evaluate comfort functions while wearing firefighters' protective gloves in dry and wet conditions at $70^{\circ}C$ air temperature with radiant heat. Four types of firefighting gloves from Korea, Germany, United States, and Japan were evaluated in both dry and wet conditions by eight male firefighters. Firefighters put their hands and forearms wearing gloves into a hands-radiant chamber that maintained at an air temperature of $70{\pm}2^{\circ}C$ ($T_a$) and globe temperature $106^{\circ}C$ ($T_g$). During the exposure, subjects followed a fixed protocol of manual movements and stopped the exposure when they felt being intolerable. Results showed that completion time was extended by 6 min on average when gloves got wet and 15 min for the Japanese gloves was extended when compared to its dry condition (p<.001); microclimate humidity on the palm at the last stage was greater for wet conditions than dry conditions in the all gloves; and skin temperatures on mid-fingertip, palm, and hand were significantly lower for wet conditions than dry conditions especially for Japanese gloves (p<.001). These results indicate that the exposure time without thermal pain to radiant heat could be extended by wetting gloves during the low radiant heat exposure.

Characterization of Cholesterol Lowering Lactic Acid Bacteria Isolated from Palm Wine and Maize Beer and Assessment of Their Use in the Production of Probiotic Papaya Juice

  • Bertrand Tatsinkou Fossi;Dickson Ebwelle Ekabe;Liliane Laure Toukam Tatsinkou;Rene Bilingwe Ayiseh;Frederic Tavea;Pierre Michel Jazet
    • Microbiology and Biotechnology Letters
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    • v.51 no.2
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    • pp.191-202
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    • 2023
  • Elevated serum cholesterol is a main risk factor for heart disorders. Most probiotic products administered to lower cholesterol are dairy products which are not suitable for lactose-intolerant individuals. In this study, we assessed the cholesterol-lowering efficacy of LAB isolated from traditionally fermented drinks in diet-induced rats and determine their efficacy in the production of non-dairy, probiotic formulations using papaya juice. LAB were isolated from palm wine and corn beer on MRS agar using a pour-plate technique. Identification was carried out using 16S rRNA gene sequencing. A hypercholesterolemia model in which diet-induced Wistar albino rats were assigned into four groups was established. Oral gavage was carried out for 30 days. On the 31st day, the rats were dissected and the serum lipid profile was analyzed using biochemical kits. A 106 cfu/ml of a 24-h-old culture of selected lactobacilli was used to inoculate papaya juice and incubated at 37℃. Microbial and chemical changes were assessed during papaya fermentation and after four weeks of cold storage. Two selected isolates (Pw1 and Cb4) had in vitro cholesterol reduction of > 80%. These two isolates lowered lipid profile (triglyceride, total cholesterol, LDL-c) significantly, and increased HDL-c levels (p < 0.5) in the rat sera. Phylogenetic analysis showed that Pw1 was 98.86% similar to Limosilactobacillus fermentum, while Cb4 was 99.54% similar to Enteroccocus faecium. Both strains fermented papaya juice with cell viability reaching 8.92 × 108 cfu/ml and 25.3 × 108 cfu/ml respectively, and were still viable after 4 weeks of cold storage.

Extrusion enhances apparent metabolizable energy, ileal protein and amino acid digestibility of palm kernel cake in broilers

  • Faridah, Hanim Shakirin;Goh, Yong Meng;Noordin, Mohamed Mustapha;Liang, Juan Boo
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.12
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    • pp.1965-1974
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    • 2020
  • Objective: This study consisted of two stages; the first was to determine the effect of extrusion and sieving treatments on the chemical properties of palm kernel cake (PKC), and accordingly, a follow-up experiment (second stage) was conducted to determine and compare the apparent metabolizable energy (AME), and protein and amino acid digestibility of extruded and sieved PKC. Methods: Two physical treatments, namely extrusion (using temperature profiles of 90℃/100℃/100℃, 90℃/100℃/110℃, and 90℃/100℃/120℃) and sieving (to 8 particles sizes ranging from >8.00 to 0.15 mm) were carried out to determine their effects on chemical properties, primarily crude protein (CP) and fiber contents of PKC. Based on the results from the above study, PKC that extruded with temperature profile 90/100/110℃ and of sieved size between 1.5 to 0.15 mm (which made up of near 60% of total samples) were used to determine treatments effect on AME and CP and amino acid digestibility. The second stage experiment was conducted using 64 male Cobb 500 chickens randomly assigned to 16 cages (4 cages [or replicates] per treatment) to the following four dietary groups: i) basal (control) diet, ii) basal diet containing 20% untreated PKC, iii) basal diet containing 20% extruded PKC (EPKC), and iv) basal diet containing 20% sieved PKC (SPKC). Results: Extrusion and sieving had no effect on CP and ash contents of PKC, however, both treatments reduced (p<0.05) crude fiber by 21% and 19%, respectively. Overall, extrusion and sieving reduced content of most of the amino acids except for aspartate, glutamate, alanine and lysine which increased, while serine, cysteine and tryptophan remained unchanged. Extrusion resulted in 6% increase (p<0.05) in AME and enhanced CP digestibility (p<0.05) by 32%, as compared to the untreated PKC while sieving had no effect on AME but improved CP digestibility by 39% which was not significantly different from that by extrusion. Conclusion: Extrusion is more effective than sieving and serves as a practical method to enhance AME and digestibility of CP and several amino acids in broiler chickens.

Portable Metamaterial Structure Antenna for Dual-Band and Polarization-Reconfigurability

  • Lee, Changhyeong;Han, Dajung;Park, Heejun;Kahng, Sungtek
    • Journal of Satellite, Information and Communications
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    • v.11 no.3
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    • pp.127-132
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    • 2016
  • This paper presents the design of a palm-sized metamaterial antenna system having reconfigurable polarization as well as dual-band characteristics. Basically, three antennas are laid by 45 degrees in order and excited by a compact metamaterial dual-band power-divider of the in-phase outputs, and the radiated fields of the antennas are mixed to turn the vector of the polarization to another. The validity of the proposed method is verified by observing the in-phase outputs from the odd-numbered power-dividing device for both 900 MHz and 2.4 GHz, and checking the changeable polarization with the antenna gain over 2 dBi for all the polarizations.

Redescriptions of Two Ampithoid Amphipods, Ampithoe lacertosa and A. tarasovi (Crustacea: Amphipoda), from Korea

  • Shin, Myung-Hwa;Hong, Ji-Sun;Kim, Won
    • Animal Systematics, Evolution and Diversity
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    • v.26 no.3
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    • pp.295-305
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    • 2010
  • Two ampithoid species, Ampithoe lacertosa Bate and A. tarasovi Bulycheva, are redescribed. A. tarasovi has been misidentified as A. lacertosa in Korea. A. tarasovi can be distinguished from A. lacertosa in having the following characteristics: the apical and medial lobes of outer lobe are separated in the lower lip, the carpus of male gnathopod 1 is about 1.4 times as long as the propodus, the palm of male gnathopod 2 has a sloped quadrate hump and a posterodistal tooth, the peduncle of uropod 3 is approximately 2 times as long as the rami, and the length of the carpus is longer than that of the propodus of female gnathopod 1. This study provides detailed descriptions and figures for both species. A. tarasovi is reported for the first time as a member of the Korean fauna.