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Effect of Heparin-binding Epidermal Growth Factor (HB-EGF) on Integrin $\alpha_{\nu}-\betaFe_3$ Expression in Preimplantation Mouse Embryos

  • Lim, Jung-Jin;Shin, Hyun-Sang;Lee, Ji-Won;Kang, Sue-Man;Lee, Sung-Eun;Kang, Han-Seung;Kim, Moon-Kyoo
    • Proceedings of the Korean Society of Embryo Transfer Conference
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    • 2002.11a
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    • pp.102-102
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    • 2002
  • Heparin-bindin epidermal growth factor (HB-EGF) is one of the EGF family to be expressed at the time of implantation in the mouse uterus. Although HB-EGF has been shown to stimulate the development of embryo and uterus in the mouse, its correlation between cell adhesion molecules remains undefined. Integrin $\alpha$$_{ν}$$\beta$$_3$, one of the cell adhesion molecules, is an important mediator of cell-substratum and cell-cell adhesion in implantation. In the present studies, we investigated the effects of HB-EGF on the embryonic development, initiation of implantation and expression of integrin $\alpha$$_{ν}$$\beta$$_3$ in in vitro culture, blocking of HB-EGF, RT-PCR and immunofluores cence analysis. The results showed that HB-EGF significantly improved the developmental rate of hatched embryos (24.1%, p<0.01) and outgrowth embryos (42.5%, p<0.01). On the other hand, this growth factor showed no offset before the hatching embryonic stage. Analysis of RT-PCR showed that HB-EGF upregulated the expression level of integrina $\alpha$$_{ν}$$\beta$$_3$ subunit genes on the preimplantation embryo and outgrowth of blastocyst (120hr and 144hr after hCG injection). Immunofluorescence analysis showed that the integrin $\alpha$$_{ν}$$\beta$$_3$ subunits localized at the pericellular borders and cell-cell contact areas. Increase in fluorescence intensity was observed in the HB-EGF treated embryos. Intrauterine injection of an anti-HB-EGF antiserum at day 3 significantly decreased the number of implantation sites (14.4, p<0.01) and significantly increased the number of recovered embryos(6.4, p<0.05) at day 5. From these results, it imply that HB-EGF improve the embryo development and accelerated the expression of integrin $\alpha$$_{ν}$$\beta$$_3$ in the preimplantation mouse embryos.

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Increasing Transnational Threats and Terrorism and Establishment of Integrated Border Security Systems: Focused on U.S., Canada and Australia (초국가적 위협 및 테러리즘 증가와 통합국경안보체계 구축: 미국, 캐나다, 호주를 중심으로)

  • Yoon, Taeyoung
    • Convergence Security Journal
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    • v.17 no.4
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    • pp.69-78
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    • 2017
  • Since the September 11, 2001, transnational crimes and terrorism have increased, the importance of border security has been emphasized and integrated CIQ capability has been required. The U.S., Canada, and Australia are consolidating CIQ to strengthen border security, focusing on strengthening travelers and goods immigration control and airports, ports and land border security. In 2003, the U.S. established the Customs and Border Protection(CBP) under the Department of Homeland Security. Canada also established the Canada Border Services Agency(CBSA) under the Public Safety Canada in 2003. The Australian Customs and Border Protection Service was integrated with the Department of Immigration and Border Protection(DIBP) and the Australian Border Force was established in 2015. However, Korea operates a distributed border management system for each CIQ task which is unable to respond to complex border threats such as illegal immigration, entry of terrorists, smuggling of drugs, and gun trade in the airports, ports and land borders. In order to solve this problem, it is possible to consider integrating sequentially the customs and quarantine services which have high similarities, and to integrate the entire CIQ tasks with the Korea Customs Service delegated to the immigration control duties in the mid to long term. There is also a plan to benchmark the CIQ single accountability agencies in the U.S., Canada, and Australia in accordance with the Korean situation and to establish a new integrated border security organization.

Effect of Different Spray Dried Plasmas on Growth, Ileal Digestibility, Nutrient Deposition, Immunity and Health of Early-Weaned Pigs Challenged with E. coli K88

  • Bosi, P.;Han, In K.;Jung, H.J.;Heo, K.N.;Perini, S.;Castellazzi, A.M.;Casini, L.;Creston, D.;Gremokolini, C.
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.8
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    • pp.1138-1143
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    • 2001
  • A total of 96 piglets were weaned at 19 and 13 days in Exp. 1 and 2, respectively, and allotted to one of four diets: three with different spray dried plasmas (SPs) and one with hydrolysed casein (HC). SPs were from pigs (SPP), mixed origin (SMP), and mixed origin with standardized level of immunoglobulins (SMPIG). All the diets contained 1.7% total lysine, 25% of the test protein source, 45% corn starch, 15% lactose, 2% sucrose, 7% soybean oil. At d 4 and d 2 in Exp. 1 and 2, respectively, piglets were perorally challenged with $10^{10}$ CFU E. coli K88. Growth performance, immunity, and health condition were measured for 15 days and 14 days in Exp. 1 and 2, respectively. To investigate apparent ileal digestibility and nutrient deposition, all piglets were sacrificed at d 14 in Exp. 2. In 1. 3 piglets died in HC diet and 1 in SPP diet. HC diet showed higher mortality (p<0.01) than other diets. In Exp. 2, no clinical sign of infection was detected, no difference for the content of E. coli K88 was found in feces at 4 and 6 days after the infection, and no E. coli K88 was found in the jejunum at the end of experiment. In both experiments, feed intake was lower for HC diet and ADG was 96, 106, 122 and 155 for HC, SPP, SMP and SMPIG diet, respectively (HC vs others, p<0.05; SMPIG vs other SP, p<0.01). Heal apparent digestibility of nitrogen in sacrificed piglets was higher for HC diet (p<0.05). After the challenge, K88-specific titers in saliva (Exp. 1) and in plasma (Exp. 2) were reduced in SMP and SMPIG. The piglets positive to the adhesion of the used E. coli strain to the intestinal brush borders had a significantly reduced growth (p<0.01) and a higher K88-specific IgA titer in plasma, in comparison with negative ones. This effect was independent of the diet. The data show the relevance of spray dried plasma sources and particularly of SP with standardized level of immunoglobulins for the feeding of early-weaned at the risk of infection by enterotoxigenic bacteria.

Farm Land Use Classification for the Planning of Planting of Eucalyptus Spp. at Mato Grosso do Sul of Brazil Using Remote Sensing and Geographic Information System (브라질 Mato Grosso do Sul 주에서의 유칼리나무 식재계획(植栽計劃)을 위한 농장토지이용구분(農場土地利用區分)에 관한 연구(硏究) - 원격탐사기술(遠隔探査技術)과 지리정보(地理情報)시스템(GIS)의 적용(適用) -)

  • Woo, Jong-Choon;Nobrega, Ricardo Campos;Imana-Encinas, Jose
    • Journal of Korean Society of Forest Science
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    • v.88 no.2
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    • pp.157-168
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    • 1999
  • This paper analyzed vegetation and land use classification, slope and permanent preservation and legal reserves on the farm Jangada and Jamaica-Mato Grosso do Sul, Brazil, using satellite image for assisting the planning of planting Eucalyptus spp. This part of the State of Mato Grosso do Sul represents an important geopolitcal area, since it is located on the borders of Bolivia and Paraguay. Also exportation of goods can be achieved through hydrovias extending to Buenos Aires, Argentina-through the Paraguay River. Also there are road and railroad connection which link the soutreastern part of Brazil to the Andean countries. The vegetation map from sheet SF 21-Campo Grande of the RADAMBRASIL Project was used as the basis for the preliminary interpretation of coverage, and complemented by a visit of the field. After the initial interpretation of the image, definition of classes of use and land occupation were made, and files of spectral signatures were created. On the farms Jamaica and Jangada Open Arboreal Savanna and Grass Savanna are the predominant physiognomies occupying 68% of total area. In spite of the results being satisfactory at the present moment, the development of this project should be revised and adjusted based on the evaluations already made, including a greater detailing of environmental components, principally with respect to soil and topography.

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A Study on the application of design in field research methods of Land Characteristic Survey for Individual Land Prices (개별공시지가 토지특성조사를 위한 현장조사방법 설계 적용에 관한 연구)

  • Lee, Seong-Kyu;Bae, Sang-Keun;Jung, Dong-Hun
    • Journal of Cadastre & Land InformatiX
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    • v.44 no.2
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    • pp.73-90
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    • 2014
  • The Officially Announced Land Price System has a limit, that is required to be able to reflect changes in land constantly every year, to implement Land Characteristic Survey for calculating land price during a specific period and human resources with limited. The purpose of this study is to apply the 'National Territory Space Usage status Survey' method to survey part of the territorial feature status information inside of selected the target sites, considering the core survey items (land category, the state of land use, altitude difference, standard site inclusion, etc) in the areas surrounding Yeonshinnae Station in which three dongs (Galhyeon-dong, Daejo-dong, Bulgwang-dong) of Eunpyeong-gu, Seoul share borders with. Based on the given budget, the manpower and period was taken into consideration to sort a total of 2,041 lots and conduct surveys on all sites. This study will be able to diagnose the efficient idle human resource utilization and work process construction plan through pilot projects specialized for providing real estate information services in preparation for cases in which national territory information survey projects that provide various business model, as well as major future core projects of the corporation will be carried out.

The research of new multimedia design development on Internet - Focus on the color - (인터넷에서의 뉴멀티미디어 디자인 개발에 관한 연구 - 색채를 중심으로 -)

  • 류성현;신계옥;이은주;이현주
    • Archives of design research
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    • v.11 no.1
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    • pp.143-152
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    • 1998
  • Recently, rapidly increa~ing internet Websites are providing us with the new kinds of multimedia infonnations without borders acting as the center for exchanging informations. Such new media infonnations through the internet passes infonnations via light on the monitor and provides the various infonnations, with the differentiation from the traditional printing media, it can be searched with electronic commands in limited space. In the process of adapting the new technologies, new media has successfully responded to the fast change and the development of its needs by experiencing the trials and errors, steadily establishing the stable position with its new infonnation transferring and exchanging methods. The representative hompage of websites of information lransfonnations means the first page containing no lower directories and consist of titles, icons, symbols and addresses and can design them in consideration of graphical process, planning, contents and others. Such hompages are very important since the graphical images shows its visual expressions deciding the total meanings of the hompages. In this research, we have analyzed the relationships between its overall colors and text colors from randomly picked hompages of websites in the internets of various areas. Generally, the homepages are designed with graphical expressions in personal way and the feedbacks and responses of such may differs, but this can be used as reference materials for the analysis of new media in objective way. Also, it can be used as the base informations for arrangement and planning of designs with the characteristics of graphics and Graphical User Intertilces in the backhlfound which are implemented over internet.

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A STUDY OF MICROLEAKAGE AND PENETRATION ABILITY OF A PIT AND FISSURE SEALANT APPLIED ON CARIOUS FISSURES (우식성 열구에 적용한 치면열구전색제의 미세누출과 침투도에 관한 연구)

  • Im, El;Lee, Sang-Ho;Lee, Nan-Young
    • Journal of the korean academy of Pediatric Dentistry
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    • v.37 no.3
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    • pp.345-351
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    • 2010
  • The purpose of this study is to compare microleakage and penetration depths of sealants applied in carious and sound fissures. Extracted premolars(n=80) were divided into 4 groups according to caries status. 1; sound, 2; stained, 3; initial caries, 4; enamel caries. Sealants were applied to the occlusal groove as per manufacturers' instructions, and specimens were thermocycled, stained, sectioned, and examined for microleakage and penetration ability. The results of the present study are as follow: 1. It showed significantly higher microleakage scores in group I than group Two, Three, Four(p<0.05), but no significant difference was found between group Two, Three, and IV(p>0.05). 2. It showed significantly higher penetration scores in group Four (p<0.05), followed by group One, Two, Three, but no significant difference was found between group One, Two, and Three(p>0.05). Based on the results of present study, when the borders of the fissure sealant are on carious enamel, a significantly higher microleakage must be expected. It is considered that depth of enamel caries in the fissure should be taken into account when applying a fissure sealant.

The Changes in the Characteristics and Distribution of Maquiladora Industries in Mexico (멕시코 마낄라도라산업의 특성과 분포 변화)

  • Kim, Hee-Soon
    • Journal of the Economic Geographical Society of Korea
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    • v.11 no.2
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    • pp.251-271
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    • 2008
  • Maquiladora industries have grown due to the decrease in labor costs caused by Mexico's economic crisis and the increase in possibility of Mexico's advance into North American markets caused by the NAFTA that come into effect since the 1980s and 1990s. Early Marquiladora industries have started to be located in the Northern borders of Mexico using young-female labor forces centered on the textile and electronic part industries. However, after the 1980s, the port soared, and the regional range of Maquiladora industries has also enlarged to 25 states. The most important regions of Maquiladora industries in Mexico are Chihuahua and Baja California and their cities are Ciudad Juares and Tijuana. Maquiladora industries had grown in terms of the cost of product and the employment until the end of the 1990s. However, Maquiladora industries have decreased in the cost of product and the employment since the 2000s. The regional range of Maquiladora industries has enlarged into the entire of Mexico, but most of Maquilador industries is still located in Northern border regions centered on six states. The textile industry is a representative one of Maquiladora industries and the early Maquilador industries have been focused on the textile industry. Thus, the textile industry in Maquiladora shows the same pattern as any other industries in Mexico. However, machinery and electronic part industries have been concentrated on the Northern border states and existing manufacturing zones. In terms of the change in employment by industry, machinery and electronic part industries occupied most high employment proportion and the textile industry sector was the next. The distinguished point is that service industries are growing.

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Histochemical and electron microscopical study on the tracheal development in fetuses and neonates of Korean native goats 1. Light microscopic, scanning and transmission electron microscopical studies (한국재래산양의 태아 및 신생아 기관의 조직발달에 관한 조직화학적 및 전자현미경적 연구 1. 광학현미경적 주사 및 투과전자현미경적 연구)

  • Kim, Chong-sup
    • Korean Journal of Veterinary Research
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    • v.37 no.1
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    • pp.87-101
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    • 1997
  • The development of trachea in fetuses on 60, 90 and 120 days of gestation and neonates of Korean native goats was investigated by microscopy and scanning and transmission electron microscopy. The results were summarized as follows; Light microscopic studies: 1. In the 60-day-old fetuses, the tracheal walls were differentiated and divided into four layers of the mucosa, submucosa, muscle and cartilage, and adventitia. The tracheal epithelium is composed of stratified ciliated columnar epithelium at 60- and 90-day-old fetuses while the epithelium observed at 120-day-old fetuses was pseudostraified ciliated colummar epithelium. 2. In the 90-day-old fetuses, tracheal glands extended into the submucosa and peripheral area of the tracheal cartilage. The blood vessels were observed in the submucosa and adventitia. The elastic and collagenous fibers were observed in the tracheal walls. 3. In the neonates, the tracheal walls consisted of mucosa with well-developed folds, submucosa, tracheal glands, muscle and cartilage, collagenous and elastic fibers, and adventitia, which were more developed than those of 120-day-old fetuses. The tracheal epithelium was developed as that in adults. Scanning electron microscopic studies: 4. In the 60-day-old fetuses, most of tracheal epithelial cells were nonciliated but short microvilli were sporadically observed on the luminal surface. On rare occasions, a few cells have solitary cilium. 5. In the 90-day-old fetuses, the ciliated cells appeared increasingly and cilia elongated longer than those of 60-day-old fetuses. 6. In the 120-day-old fetuses, the nonciliated cells covered with microvilli in dome-shape were barriered by thick carpet of cilia. The nonciliated cells also have many papillary projectons on the apical surface. 7. In the neonates, the nonciliated cells in tracheal epithelium were covered compactly with numerous cilia, and many secretory droplets were found on the cilia. Transmission electron microscopic studies: 8. In the 60-day-old fetuses, nonciliated cells of the tracheal epithelium contain large amounts of glycogen granules in the supernuclear and subnuclear areas meanwhile a few cell organelles were formed. Cilia were well formed along the apical cell membranes of the ciliated cells. Also found in the ciliated cells were basal corpuscles, mitochondria and short chains in granular endoplasmic reticulum(GER). Between the epithelial cells presented were well-defined junctional complex with zonula occludens and desmosomes. The nuclei were variable in size and shape. The more developed nucleoli were observed conspicuosly. 9. In the 90-day-old fetuses, nonciliated cells contained large glycogen granules. Accumulated glycogen granules were observed in the subnuclear and supranuclear portion of the cytoplasm. A few short microvilli were covered with glycocalyx. Ciliated cells contained numerous mitochondria and short chains of GER. 10. In the 120-day-old fetuses, the ciliated cells contained numerous mitochondria, abundant short chains of GER and nucleoli. Nonciliated cells contained some Golgi complex and mitochondria. The cell borders were well-defined and distinct junctional complex with zonula occludens, desmosomes, and interdigitorum. 11. In the neonates, well-developed goblet cells were observed in the tracheal epithelium. Ultrastructures of ciliated and nonciliated cells on the tracheal epithelia were similar in pattern as those in adults.

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First Report of Curvularia Leaf Blight Caused by Curvularia trifolii on Creeping Bentgrass in Korea (Curvularia trifolii에 의한 크리핑벤트그래스 잎마름병 발생)

  • Sung, Chang-Hyun;Koo, Jun-Hak;Kim, Jung-Ho;Yoon, Jung-Ho;Lee, Jung-Han;Shim, Kyu-Yul;Kwak, Youn-Sig;Chang, Seog-Won
    • Weed & Turfgrass Science
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    • v.5 no.2
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    • pp.101-104
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    • 2016
  • Curvularia leaf blight of creeping bentgrass (Agrostis stolonifera) putting green by caused Curvularia trifolii was observed in Hapcheon, Korea. In July to September 2014, curvularia leaf blight developed on leaf blades of creeping bentgrass as small water-soaked lesions that subsequently turned into dark-colored, necrotic spots. The spots were expanded and became gray, grayish-brown, or light brown, circular to oblong lesions with purple to dark brown borders that often were surrounded by a yellow halo. The necrotic lesions coalesced, became irregular in shape and caused tip or complete blighting of the leaves. Blighted leaf blades appeared grayish-white to tan. The fungus was identified by morphological characters and 16S rDNA sequencing as C. trifolii. Conidia of the pathogen were short, with predominantly 3-septa, straight or often curved, with end cells frequently paler than intermediate cells. Size of the 3-septate conidia in culture are $26{\sim}28{\times}11{\sim}12{\mu}m$. Pathogenicity of the fungus was proved by artificial inoculation on the host. This is the first report of C. trifolii causing leaf blight on creeping bentgrass in Korea.