• Journal of Nutrition and Health
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• 제25권7호
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• pp.569-577
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• 1992

To study the effects of the age and the dietary protein content on Ca metabolism male rats of 1 month 6 month 12 month of age were fed experimental diets containing 5%, 15% or 50% casein for 4 weeks. Food and ca intake were higher in old rats and in high protein groups. The weight ash and Ca contents of femur and tibia were higher in old rats. The higher dietary protein level resulted in higher skeletal weigh ash and Ca contents. But high protein diet(50% casein) lead to reduced bone mineral density(ash/dry bone weight) and Ca density(Ca/dry bone weight) in 1 month old rats. Low protein diet(5% casein) on the other hand reduced the bone growth even though the bone density was higher in this group. The ill effect of low protein diet was not evident in 12 month old rats. Glomerular filteration rate(GFR) and urinary Ca excretionincreased with age and with dietary protein level especially in 12 month old rats. Serum immunoreactive parathyroid hormone(iPTH) level tended to be higher in aged rats but was not affected by dietary protein level except 1 month old rats where 50% protein group showed significantly higher value. This study showed that the dietary protein level seemed to have different effect on Ca metabo-lism in rats of different age., The low bone density in the high protein group of growing rats may be due to the higher iPTH level and increased urinary Ca. The dietary protein level however had no effects on the bone composition in aged rats even though the higher urinary Ca excretion. In conclusion this study suggests that high protein intake from young may lead to less peak bone mass and to increase the bone loss in later years, which would increase the risk for osteporosis.

• Preventive Nutrition and Food Science
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• 제8권3호
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• pp.245-248
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• 2003

This study was undertaken to investigate the effects of the Ginseng radix in osteoporosis of the oophorectomized rats. In this experiment, the rats were oophorectomized and administered the water extracts of the Ginseng radix. The spinal bone mineral density, calcium, phosphorus and ash weight of the bones were measured. The spinal bone mineral density was significantly increased in the ovariectomized (OVX)-Ginseng radix group at 8 weeks as compared to the OVX-saline group. Furthermore, the calcium and phosphorus contents of the femoral and fibula-tibia were significantly increased in the OVX-Ginseng radix group as compared to the OVX-saline group. The ash weights of the femoral and fibula-tibial bones were increases in the OVX-Ginseng Radix group, although it was not statistically significant. On reviewing these experiments, it appears that the Ginseng radix possess efficacy for the prevention of osteoporosis. Further study would be of value to confirm the efficacy of the Ginseng radix for the treatment and/or prevention of osteoporosis in humans.

• Journal of Nutrition and Health
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• 제31권4호
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• pp.716-728
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• 1998

To investigate the effect of dietary Ca levels on metabolic changes of Ca and skeleton in postmenopausal women, 10-month-old ovariectomized female rats were compared with 2 month old rats. The rats were fed either 0.2% or 1.2% Ca diets for 16 weeks. Food intake and weight gain as higher in rats fed high Ca diets and in ovariectomized rats. Apparent Ca absorption as higher, and Ca balance was lower in the low Ca groups. Vertebrae density was higher in old rats or those fed a high Ca diets. The old rats and ovariectomized rats showed decreased bone formation, increased bone resorption and kidney function deterioration resulting in increased urinary Ca excretion. Contradictory to the above observation, old rats and ovariectomized rats still showed higher bone mass and bone ash content. Therefore aging was not fully onging in 10-month-old rats. Bone weights, mineral contents, and mineral/wt ratio were lower in ovariectomized rats. Dietary Ca level did not affect urinary Ca excretion, urinary protein excretion, GFR, serum alkaline phosphatase, or urinary hydroxyporline excretion. This means that dietary Ca level did not influence kidney function or bone turnover. However Ca content and the ash content of femur, 4th vertebra, and scapula were increased in high Ca groups. Therefore, it is considered that decreased bone formation and accelerated bone resorption may account for the increased osteoporotic risk in women in menopause after middle age. However, Ca metabolism can be improved and bone components can be maintained if Ca is supplemented.

• 한국가금학회지
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• 제23권4호
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• pp.169-175
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• 1996

An experiment was conducted with male broiler chicks to determine the effect of different dietary phosphorus sources and evaluate the biological availability of phosphorus sources. The biological availability of phosphorus from dicalcium phosphate (DCP) was used as a reference standard (100%) compared to defluorinate phosphate (DFP). DCP and DFP was supplemented to a corn-soy basal diet at levels of 0.05, 0.15, 0.25, 0.35%. Each of 24 pens of 10 male broiler chicks with three replications was used for three weeks. The results indicated that weight gain, feed in-take, feed efficiency, nonphytic phosphorus (NPP) and tibia ash were significantly different among treatments. Dietary supplemental phosphorus of DFP improved weight gain, NPP intake and feed efficiency consistently, whereas supplements of DCP did not show consistent increase. Regression equations was used for the availability of DPP compared with DCP when percent bone ash was a function of total phosphorus in the diet. The percent (%) bone ash of DFP groups compared to that of DCP groups showed a value of 59.98% as a slope ratio. DFP indicated lower biological availability compared to DCP, but it's dietary supplementation tended to increase bone ash and maximize the growth of young broiler chicks.

• 한국농촌생활과학회지
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• 제3권
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• pp.27-35
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• 1992

• Animal Bioscience
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• 제36권9호
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• pp.1393-1402
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• 2023

Objective: In this study effects of three types of beddings on broiler leg health and bone biomechanics were evaluated. Methods: A total of 504 male chicks (Ross 308) were randomly placed on three beddings (4 replicates/group; 42 birds/pen), zeolite-added litter (ZL), plastic-grid flooring (PF), and wood shavings (WS). On day 42, chickens were weighed, slaughtered, and samples (bone, muscle, and drumstick) were collected. Bones were subjected to leg health tests, morphometric measurements, biomechanical testing, and ash analysis. Results: Broilers in PF and WS groups showed higher live weight than the ZL group (p<0.001), and the incidence of tibial dyschondroplasia (TD) and varus valgus deformity due to distal bending was significantly higher in PF (p<0.001). Multinomial logistic regression showed that bedding has a significant (p = 0.038) contribution toward the development of TD. Tibial strength (p = 0.040), drumstick width (p = 0.001), and total femur and epiphyseal ash contents (p = 0.044, 0.016) were higher in the ZL group. Chicken live weight was correlated with tibial length and weight (r = 0.762, 0.725). Conclusion: Flooring and the type of bedding material directly affect broiler bone length, strength and leg health. Plastic bedding improves the slaughter weight of chickens on the expense of leg deformities, and zeolite litter improves leg health and bone strength.

• 한국식품과학회지
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• 제32권1호
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• pp.31-36
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• 2000

본 연구는 국내산 녹용(사슴뿔의 상대)을 종으로 5개 부위, 횡으로 벨벳층과 스폰지층의 2개 부위로 분할하여 무기질 조성 및 함량을 분석하였다. 회분, Ca, P, Mg, Zn 함량은 tip 부위에서 하단으로 내려갈수록 유의적으로 증가하였으며, Mn, Cu, Fe 함량은 tip 부위에서 하단으로 내려갈수록 유의적으로 감소하였다. 스폰지층은 벨벳층에 비하여 회분 함량이 3배, Ca 함량이 2배 정도 높았으며 대부분의 무기질 함량은 벨벳층에 비하여 스폰지층이 유의적으로 높았다. Tip부위는 다른 부위에 비하여 특히 회분, Ca, P, Cu, Fe 함량 및 Ca/ash(%), Ca/P, Ca/Fe 비에 있어서 현저한 차이를 보였으므로 이러한 성분 및 성분비가 녹용의 품질 지표로 사용될 수 있는 가능성이 제시되었다.

• 한국결정성장학회지
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• 제10권2호
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• pp.171-176
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• 2000

패각으로부터 얻어지는 고순도의 소석회와 탄산칼슘을 이용하여 인산1수소칼슘, 수산화아파타이트, 골회 및 인산3칼슘과 같은 인산칼슘계 화합물을 제조하였다. 인산2수소칼슘은 고순도의 소석회와 인산용액을 이용하여 제조하였으며, 그리고 인산1수소칼슘을 출발원료로 하여 고상반응법에 의해 골회를 제조하였고 또 수열처리법을 이용하여 수산화아파타이트를 제조하였다. 인산3칼슘의 제조는 골회와 고순도의 탄산칼슘을 혼합한 뒤 고상반응시켜 제조하였다. 본 연구에서는 이상과 같은 인산칼슘계 화합물에 대한 최적의 제고공정 및 제조 조건을 확립하였다.

• 대한한방부인과학회지
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• 제19권1호
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• pp.47-68
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• 2006

Purpose : This study was carried out to investigate the effects of Gagamdokhwalgisang-Tang on the morphometric changes of femur, and on the hormones and cytokines associated with bone metabolism in overiectomized rats. Methods : Twenty-four female Sprague-Dawley rats were divided into sham operated group(normal) ovariectomized group(control), and treated with extract of GD group(treated). Each group was evaluated the changes of body weight at 0, 3, 6, 8 weeks after ovariectomy. Morphometric analysis(femur weight, femur/body weight ratio, femur ash weight femur ash/body weight ratio cross sectional area of compact bone and concellous bone of femur) and histopathological examination were performed at 8 weeks after ovariectomy. Estrogen, Alkaline Phosphatase(ALP) and cytokine(Tumor necrosis $factor-{\alpha}$, $Interleukin-l{\beta}$, Inerleukin-6) assay were performed at 8 weeks after ovariectomy. Results : 1. The body weight of control and treated group was significantly increased(p<<0.001) compared with the normal group at 8 weeks. 2. The femur weight and femur/body weight ratio of treated group were significantly increased(p<<0.05, p<<0.01) compared with the control group at 8 weeks. 3. The femur ash weight showed no significantly different changes, but femur ash/body weight ratio of treated group was significantly increased(p<<0.05) compared with the control group at 8 weeks. 4. In the cross sectional area of cancellous bone of femoral body, the treated group was significantly increased(p<<0.001) compared with the control group at 8 weeks. 5. The serum estrogen level of treated group showed no significantly different changes compared with the control group at 8 weeks. 6. The serum ALP activity of treated group was significantly decreased(p<<0.01) compared with the control group at 8 weeks. 7. The serum Tumor necrosis $factor-{\alpha}(TNF-{\alpha})$ level of treated group was significantly decreased(p<<0.05) compared with the control group at 8 weeks. 8. The serum $Interleukin-l{\beta}(IL-1{\beta})$ level of treated group was significantly decreased(n<<0.001) compared with the control group at 8 weeks. 9. The serum Interleukin-6(IL-6) level of treated group was significantly decreased(P<<0.01)compared with the control group at 8 weeks. Conclusion : These results indicate that GD inhibits bone resorption in ovariectomized rats. And the major inhibitory mechanism may be related to the inhibitory effects of GD on the secretion of $TNF-{\alpha}$, $IL-1{\beta}$ and IL-6 in the pathogenesis of osteoporosis in estrogen deficient rats.

• Applied Biological Chemistry
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• 제41권1호
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• pp.67-72
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• 1998

수산가공 부산물을 식품소재와 같이 효율적으로 이용하기 위한 일련의 기초 연구로 6종의 어류뼈(대구뼈, 명태뼈, 각시가자미뼈, 민태뼈, 붕장어뼈 및 고등어뼈)에 대하여 식품 성분 특성을 검토하였다. 건물당 조단백질 함량, 콜라겐 함량 및 amino acid 조성비는 민태뼈가 각각 40.7%, 5.86% 및 189 잔기/1,000 잔기로 기타 어류뼈의 $28.8{\sim}38.8%$, $39.4{\sim}5.73%$ 및 $160{\sim}170$ 잔기/1,000 잔기 보다 높았으나, 가축뼈 콜라겐의 amino acid 조성비(204 잔기/1,000 잔기) 보다는 낮았다. 각시가자미뼈, 붕장어뼈 및 고등어뼈의 경우 건물당 조지방 함량은 $22.8{\sim}43.9%$이었고, 생리작용을 가지고 있는 EPA 및 DHA 등의 조성비는 $15.6{\sim}23.8%$로 오징어 내장의 그 성분들보다 낮았다. 어류뼈의 무기질은 대부분 칼슘$(37.1{\sim}38.6%)$및 인$(18.0{\sim}18.5%)$으로 구성되어 있었고, 이들의 함량은 대구뼈(38.1% 및 18.5%) 및 명태뼈(38.6%, 18.3%)가 다른 어류뼈는 물론이고, 가축뼈보다 많았다. 이상의 결과로 미루어 보아 콜라겐 또는 젤라틴 추출 소재로는 민태뼈가, 칼슘 추출소재로는 대구뼈 및 명태뼈가 적절 하였다.