• Journal of Acupuncture Research
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• v.20 no.3
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• pp.34-44
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• 2003

Objective : Bone homeostasis is maintained by balance of bone formation and resorption. Therefore, bone related diseases arose by disturbance of this balance between osteoblast and osteoclast activities. To develop a successful screening system the therapeutic components based on oriental medicine is essential to set up systematic approach for that purpose. The purpose of this study is to the know the gene expression using cDNA microarray assay. Methods : Cervi Pantotricuhum Cornu Herbal-acupuncture extract was prepared by boiling. human osteosarcoma cells(HOS) were treated with Cervi Pantotricuhum Cornu Herbal-acupuncture solution. Then mRNA was extracted and cDNA microarray assay was performed. Results : Human osteosarcoma cells(HOS) treated with Cervi Pantotricuhum Cornu Herbal-acupuncture solution($500{\mu}g/m{\ell}$) showed that thioredoxin, TAFII31 and two novel genes were increased. However many genes decreased their expression by Cervi Pantotricuhum Cornu Herbal-acupuncture. Conclusions : This type of approach will give a good chance to explore the favorable effects of Cervi Pantotricuhum Cornu Herbal-acupuncture. Further study is needed for investigating the effect of Cervi Pantotricuhum Cornu Herbal-acupuncture.

• BMB Reports
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• v.41 no.5
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• pp.382-387
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• 2008

It is known that the stable protein 1 (SP1) detected in aspen plants remains soluble upon boiling and that sp1 expression in transgenic aspen is resistant to salt stress. Presently, we analyzed the effect of expression of SP1 in Arabidopsis thaliana plants and their response to high temperature stress. After $45^{\circ}C$ for 16 h, relative to wild type plants, sp1 transgenic plants exhibited stronger growth and were better in several physiological properties including chlorophyII, chlorophyII fluorescence, water content, proline content, and malondialdehyde content. These preliminarily results suggest that the over-expression of SP1 may notably enhance heat-tolerant level of transgenic A. thaliana plants.

• Korean Journal of Microbiology
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• v.11 no.2
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• pp.101-106
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• 1973

The microwave of 2450 MHz, generated by a household cooking oven, was evaluated for its applicability to melt various rehydrated media and to remove dissolved oxygen from tubed media for anaerobic culture. The effect on the sterilization of E. coli in selective media was also evaluated. The following results were obtained. 10 The microwave oven was useful in saving time for melting media and in eliminating heat and combustion gas from the laboratory, which were inevitable by-products in the conventional flame method. 2) Dissolved oxygen could be removed without boiling over by exposing the tubes of anaerobic culture medium after putting them in a wire basket in a beaker with water. 30 The count of E. coli during the melting of MacConkey and EMB agar were similar to those treated with open flame. The microwave treatment was not considered a possible mean to replace autoclaving even in these selective media.

• Membrane Journal
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• v.18 no.3
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• pp.214-218
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• 2008

Protain affinity membranes based on PES-BSA was preapared by the electrospinning method. The process problem caused by the electrospining was solved by using HFB having high solubility and boiling point. It was expecting that the mass production of protein affinity membrane would be possible with broad range of optimized temperature and humidity. BSA in the PES nanofiber was confirmed by the color change from colorless to violet during the biuret test. The buffer solution with DMSO showed that the amount of elution was 5 times higher than the one when the buffer solution without DMSO was used. This is due to the restriction effect of DMSO on the dissociation of L-tryptophan from BSA during the washing step.

• Journal of the Korean Society for Heat Treatment
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• v.23 no.5
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• pp.249-256
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• 2010

Water jet impingement cooling has been widely used in a various engineering applications; especially in cooling of hot steel plate of steelmaking processes and heat treatment in hot metals as an effective method of removing high heat flux. The effects of cooling water temperature on water jet impingement cooling are primarily investigated for hot steel plate cooling applications in this study. The local heat flux measurements are introduced by a novel experimental technique that has a function of high-temperature heat flux gauge in which test block assemblies are used to measure the heat flux distribution during water jet impingement cooling. The experiments are performed at fixed flow rate and fixed nozzle-to-target spacing. The results show that effects of cooling water temperature on the characteristics of jet impingement heat transfer are presented for five different water temperatures ranged from 5 to $45^{\circ}C$. The local heat flux curves and heat transfer coefficients are also provided with respect to different boiling regimes.

• Journal of the Korean Society for Heat Treatment
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• v.18 no.6
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• pp.375-382
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• 2005

This paper presents the results of experimental investigation for the effect of heat conduction on the evaporation cooling of water droplet in the process of heat treatment. The experiments are mainly focused on the surface temperature, the surface roughness and the droplet diameter at aluminum. The range of surface temperature is from $80^{\circ}C$ to $140^{\circ}C$, surface roughness is from $R_a=0.18{\mu}m$ to $R_a=1.36{\mu}m$ and droplet diameter is from 2.4 mm to 3.0 mm. The results show that the total evaporation time is shorter for the larger surface roughness, the time averaged heat flux has maximum value for the larger surface roughness and exist the critical heat flux. The total evaporation time has a big influence on the evaporation region for the smaller droplet size, but the total evaporation time has not influence on the nuclear boiling region.

• Archives of Pharmacal Research
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• v.26 no.2
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• pp.157-161
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• 2003

The major ingredient of Persicae Semen is a cynogenic compound, amygdalin (D-mandelonitrile-$\beta$-gentiobioside). Controversial results on the anticancer activity of amygdalin were reported due to its conversion to its inactive isomer, neoamygdalin. In order to inhibit the epimerization of amygdalin, we used newly developed simple acid boiling method in preparation of Persicae Semen extract. HPLC analysis revealed most of amygdalin in Persicae Semen extract was active D-form. Persicae Semen extract was used to analyze its effect on cell proliferation and induction of apoptosis in human promyelocytic leukemia (HL-60) cells. Persicae Semen extract was cytotoxic to HL-60 cells with $IC_{50}$ of 6.4 mg/mL in the presence of 250 nM of $\beta$-glucosidase. The antiproliferative effects of Persicae Semen extract appear to be attributable to its induction of apoptotic cell death, as Persicae Semen extract induced nuclear morphology changes and internucleosomal DNA fragmentation.

• Nuclear Engineering and Technology
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• v.39 no.5
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• pp.627-636
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• 2007

The present work is aimed at further discussing the effectiveness of dimensionless parameters recently proposed for the analysis of flow stability in heated channels with supercritical fluids. In this purpose, after presenting the main motivations for the introduction of these parameters in place of previously proposed ones, additional information on the theoretical bases and on the consequences of this development is provided. Stability maps, generated by an in-house program adapted from a previous application to boiling channels, are also shown for different combinations of the operating parameters. The maps are obtained as contour plots of an amplification parameter obtained from numerical discretization and subsequent linearization of governing equations; as such, they provide a quantitatively clear perspective of the effect of different boundary conditions on the stability of heated channels with supercritical fluids. In order to assess the validity of the assumptions at the basis of the in-house model, supporting calculations have been performed making use of the RELAP5/MOD3.3 computer code, detecting the values of the dimensionless parameters at the threshold for the occurrence of instability for a heated channel representative of SCWR proposed core configurations. The obtained results show reasonable agreement with the maps, supporting the applicability of the proposed scaling parameters for describing the dynamic behaviour of heated channels with supercritical fluids.

• Journal of the Korean Solar Energy Society
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• v.23 no.4
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• pp.37-43
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• 2003

The aim of this research is to study the feasibility of the solar(hot fluid) driven $NH_3/H_2O$ absorption chiller, made by re-manufacturing of Gas fired $NH_3/H_2O$ absorption chiller. This experimental study is performed with the temperature of the inlet hot fluid of generator. In order to determine the inlet temperature of the generator, which gives maximum COP, the experimental data are obtained with various hot fluid supply temperature in range of $130\sim170^{\circ}C$. Remodeled chiller is operated with periodical cooling effect, which due to mixture subcooled pool boiling, then the COP is evaluated in average. The maximum COP$(\sim0.36)$ is at $160^{\circ}C$. The temperature is stable operation temperature range of typical vacuum collector. It offers a feasibility of solar driven $NH_3/H_2O$ absorption chiller.

• Transactions of the Korean Society of Mechanical Engineers
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• v.18 no.5
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• pp.1275-1287
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• 1994

Heat transfer performance improvement by fin and grooves is studied for condensation of R-11 on integral-fin tubes. Eight tubes with trapezoidal shaped integral-fins having fin densities from 748 to 1654 fpm and 10, 30 grooves are tested. A plain tube having the same diameter as the finned tubes is also tested for comparison. R-11 condenses at saturation state of $32^{\circ}C$ on the outside tube surface cooled by inside water flow. All of test data ate taken at steady state. Beatty and Katz's, Rudy's and Webb's theoretical models are used to predict the R-11 condensation coefficient of tubes having 748, 1024 and 1299 fpm. The predicted value by Betty and Katz's model is within 10% of experimental values in this study at fpm<1024 and Rudy's model predicted the experimental data at fpm>1024 within 15%. The tube having fin density of 1299 fpm and 30 grooves has the best overall heat transfer performance. This tube shows the overall heat transfer coefficient of 11500 $W/m^{2}K$,/TEX> at coolant velocity of 3.0m/s.