• Animal cells and systems
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• v.11 no.2
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• pp.175-182
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• 2007

The lysozyme II gene of cabbage butterfly Artogeia rapae was cloned from fat body of the larvae injected with E. coli and its nucleotide sequence was determined by the RACE-PCR. It has an open reading frame of 414 bp nucleotides corresponding to 138 amino acids including a signal sequence of 18 amino acids. The estimated molecular weight and the isoelectric point of the lysozyme II without the signal peptide were 13,649.38 Da and 9.11, respectively. The A. rapae lysozyme II (ARL II) showed the highest identity (81%) in the amino acid sequence to Manduca sexta lysozyme among other lepidopteran species. The two catalytic residues ($Glu^{32}$ and $Asp^{50}$) and the eight Cys residue motifs, which are highly conserved among other c-type lysozymes in invertebrates and vertebrates, are also completely conserved. A phylogenetic analysis based on amino acid sequences indicated that the ARL II was more closely related to M. sexta, Hyphantria cunea, Heliothis virescens, and Trichoplusia ni lysozymes. The ARL II gene was expressed in Spodoptera frugiperda 21 insect cells and the recombinant ARL II (rARL II) was purified from cell-conditioned media by cation exchange column chromatography and reverse phase FPLC. The purified rARL II was able to form a clear zone in lysoplate assay against Micrococcus luteus. The lytic activity was estimated to be 511.41 U/mg, 1.53 times higher than that of the chicken lysozyme. The optimum temperature for the lytic activity of the rARL II was $50^{\circ}C$, the temperature dependency of the absolute lytic activity of rARL II was higher than that of the chicken lysozyme at low temperatures under $65^{\circ}C$.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.28 no.12
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• pp.954-963
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• 2017

In this paper, we analyzed the electric/magnetic field distribution and SAR distribution in a human body due to the resonant-type wireless power transfer(WPT) system with an operating frequency of 6.78 MHz. To analyze the field distribution under the unperturbed condition, a prototype system was fabricated and the measured results were compared with the simulation results. For safety during measurement, the available power to the transmitter coil is limited to 1 W. To analyze the induced current density and SAR distribution, a simple human model consisting of three layers, skin, fat, and muscle, was used for the simulation. The electromagnetic wave exposure levels obtained through measurement and simulation were compared with the recommended levels by the ICNIRP.

• Journal of the Korea Convergence Society
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• v.11 no.11
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• pp.405-416
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• 2020

This study was conducted to identify the factors affecting sarcopenia in middle-aged and older women in Korea. This is a cross sectional study by using 5th Korea National Health and Nutrition Examination Survey data. The data were analyzed by using the SPSS 26.0 program. The prevalence of sarcopenia was 10.8%. There were significant differences according to ASM, age, education, current job, marital status, perceived health status, energy intake, protein intake, BMI, body fat, alcohol drinking, menopause, central obesity, impaired fasting glucose and chronic disease. Woman with central obesity and osteoarthritis increased risk of sarcopenia each 4.15 times(p<.001), 3.06 times(p=.041) and energy intake decreased risk of sarcopenia 0.99 times(p=.043). In order to prevent and manage sarcopenia, strategies for managing central obesity and osteoarthritis in mddile-aged and older woman and adequate food intake are needed.

• Preventive Nutrition and Food Science
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• v.16 no.4
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• pp.283-290
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• 2011

The recent increase of colon, breast, and prostate cancer incidence in Korea has been attributed to a diet pattern change to a more Western style, in which the foods eaten are higher in protein and fat. Whether high protein intake itself stimulates tumor cell growth and exacerbates disease status has been investigated, however, many epidemiological studies have inconsistent results between meat intake and the risk of certain cancers. These inconsistent results are partly because of the difficulty of studying the effects of just the meat intake. Other factors, such as overall meal context, could not be completely excluded in the study. To address the question of whether high protein itself is independently associated with carcinogenesis, we initiated ICR mice with 200 nmol ($50{\mu}g$) 7,12-dimethylbenz[a]anthracene (DMBA) and fed animals either a normal diet (ND, 14% casein) or a high protein diet (HPD, 50% casein) for 15 weeks with 12-O-tetradecanoylphorbol-13-acetate (TPA) promotion in two-stage skin carcinogenesis protocol. There was no significant difference between ND and HPD group in food intake and body weight throughout the experiment. However, tumor multiplicity of the HPD group was decreased by 75.5% compared to that of the ND group. In addition, HPD inhibited skin hyperplasia and epidermal cell proliferation. Western analyses with whole skin lysates showed that HPD inhibited TPA-induced Akt (S473), S6K (T389), 4E-BP1 (Thr 37/46) and Erk1/2 (Thr202/Tyr204) phosphorylation as well as COX-2 expression. Taken together, these data suggest that a high protein diet has an anticarcinogenic effect by inhibiting the TPA-induced Akt signaling pathway.

• Journal of the Korean Home Economics Association
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• v.44 no.10
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• pp.67-77
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• 2006

This study was performed investigate eating behaviors and their association with obesity in Korean elderly people. A total of 9,408 (male 4,487, female 4,921) Korean adults aged 40 to 69 years were interviewed and examined from May 2001 to Feb 2002. The subjects were classified into 4 weight status groups based on body mass index (BMI, $kg/m^2$): under weight, BMI<18.5; normal, $18.5{\leq}BMI\leq24.9$; overweight, $25.0{\leq}BMI\leq29.9$; obesity, $BMI\geq30.0$. Anthropocentric parameters, eating behavior, and preference of cooking method and taste were examined. In male, distribution of weight status by BMI was under weight 2.4%, normal 58.1%, over weight 36.7%, obesity 2.8%. As the BMI increased, the rate of skipping meal, snacks, and eating out were increased and the rate of eat alone was decreased. When compared according to the weight status by BMI, the frequency of the steamed, roasted, fried, seasoned, and soup intake rates were increases in the cooking methods and preference of greasy taste was increased by degree of obesity. In female, distribution of weight status by BMI was under weight 1.4%, normal 52.8%, over weight 38.6%, obesity 7.2%. There were differences in the rate of eating out, snacks, the frequency of fried food intake rates, and preferences of salty, hot, greasy taste according to the weight groups by BMI. In this results, we suggests that keep regular meal and keep away from the high-fat, salty, stimulative foods for prevent and administer the obesity in Korean adults meal and female.

• The Korean Journal of Community Living Science
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• v.24 no.1
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• pp.85-97
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• 2013

The purpose of this study was to develop an appropriate jacket pattern for obese women in 20s and 30s, providing a good fit and appearance. The results were as follows: among 3 kinds of patterns collected in order to develop a jacket pattern to fit the average body of an obese woman in 20s and 30s, a business-type pattern(which was comparatively evaluated as a good fit) was worn and revised so as to design a final research pattern with proper fitness and a good shape. The biggest problem of the existing pattern was may overall spares, especially many spares of bust size, waist measurement, hip circumference, all of which makes a bad appearance. Considering the results of a survey which indicates that young obese women like tight-fitting clothes, bust size was decreased from 8 cm to 6 cm, and their waist measurements were bigger compared to other age groups, so a total of 1.1 cm(with 0.3 cm in the front, back side, and 0.5 cm in the back waist) was decreased. The hip circumference was decreased by 2 cm from H/2+4 to H/2+2 cm, the quantity of front deflection was established as 1.5 cm in order to compensate for the coming off of the front side caused by the fat on the stomach.

• Korean Journal of Pharmacognosy
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• v.37 no.3
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• pp.190-195
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• 2006

Obesity is associated with a number of pathological disorders such as non-insulin-dependent diabetes, hypertension, hyperlipidemia, and cardiovascular diseases. Bangpoongtongsungsankamibang (BTSK) has been widely used in the oriental medicine for the treatment of several diseases associated with inflammatory abnormalities in cardiovascular and nervous system. The BTSK is the modified prescription of Bangpoongtongsungsankamibang in which sea tangle (Laminaria japonica) were added. This study was carried out to detemine the anti-obestic effects of BTSK. Pretreatment with the BTSK at daily dose of 100 or 200 mg/kg (p.o.) far 4 weeks reduced serum triglyceride, total cholesterol contents in rat induced by Poloxamer-407 or Triton WR-1339, respectively. Furthermore, post-treatment with BTSK far four weeks also inhibited body weight gain, adipose tissue mass and hyperlipidemia induced by the high fat diet for six weeks. The BTSK shifted serum total-, HDL- and LDL-cholesterol levels toward the values of normal group, suggesting that BTSK has hypolipldemlc effects. The rats fed BTSK reduced lipid peroxide and hydroxy radical in the rat blood and increased superoxide dismutase (SOD) activity compared to the control group. Taken together, these results superoxide that BTSK improve hyperlidemia and obesity via the upregulation of anti-oxidative mechanism.

• The Korean Journal of Food And Nutrition
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• v.20 no.4
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• pp.440-449
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• 2007

The purpose of this study was to examine the effects of taurine supplementation on serum lipidperoxide(TBARS), a risk factor for cardiovascular disease. The subjects were 22 healthy middle-aged women(33 to 54 years). Serum lipids, thiobarbituric acid reactive substances(TBARS), and plasma taurine levels were measured before and after supplying 3 g of taurine per day for 4 weeks. Plasma taurine was analyzed by Dabsyl-Cl(4-dimethylamino azobenzen-4-sulfonyl-chloride) derivatization and reversed-phase HPLC. Serum TBARS was measured by the Yagi method. Daily dietary taurine intake was calculated by food frequency questionnaire method. The weight and height means of the 22 subjects were $57.9{\pm}5.2$ kg and $159.2{\pm}5.2$ cm, respectively. Their percent body fat and waist/hip ratio(WHR) were 26.8% and 0.84, respectively, which were slightly higher than the average for middle-aged Korean women. Serum TC, TG and LDL-C levels tended to decrease after taurine supplementation, but HDL-C was not changed. A positive correlation between plasma taurine and HDL-C was shown after taurine supplementation. The serum TBARS concentration was significantly decreased from $5.05{\pm}0.84nmol/d{\ell}$ to $4.17{\pm}0.64nmol/d{\ell}$ after taking taurine(p<0.01), and the plasma taurine concentration was significantly increased from $63.7{\pm}14.2{\mu}mol/{\ell}$ to $73.8{\pm}16.6{\mu}mol/{\ell}$ after taurine supplementation(p<0.05). The average dietary intake of taurine was $178.5{\pm}50.4$ mg/day, which is similar to the average daily taurine intake of Korean women. In conclusion, taurine is an effective nutrient that antagonizes TBARS levels. Therefore, this study suggests that a sufficient taurine intake may be an effective way to prevent cardiovascular disease such as atherosclerosis.

• The Korean Journal of Food And Nutrition
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• v.31 no.5
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• pp.604-615
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• 2018

The purpose of this study was to investigate the dietary habits of smokers and non-smokers among a few male university students residing in Changwon-si and factors that affect their smoking behavior. A self-administered questionnaire was conducted for a duration of one month in June 2015 (n=367). The results of this study showed that the breakfast skipping rate of the smoking group was significantly higher compared to the non-smoking group (p<0.001), and the smoking rate was significantly higher among those with more serious smoking problems (p<0.001). Compared to the non-smoking group, the smoking group showed significantly higher BMI (body mass index) (p<0.05) and demonstrated significant drinking habit of more liquid type yogurt among milk and dairy products and significantly more coffee mixes among fat, oils, and sugars (p<0.05). The results of multiple regression analysis showed the necessity to prepare smoking systems in school (t=-2.87, p<0.05), encumbrances to studies due to smoking (t=-3.59, p<0.001), and variables that affect smoking such as the number of close friends who smoke (t=4.17, p<0.001), BMI (t=3.08, p<0.05), and the level of problematic drinking (t=3.67, p<0.001). The results of this study suggest factors that must be considered in establishing smoking prevention education programs, anti-smoking policies, and strategies for university students.

• Food Science of Animal Resources
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• v.31 no.4
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• pp.530-536
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• 2011

It is well established that uncoupling protein 3 (UCP3) is expressed largely in skeletal muscle, white adipose tissue and brown adipose tissue and has been suggested to play important roles in regulating energy expenditure, body weight, thermoregulation as well as fatty acid metabolism and obesity. Therefore, the UCP3 gene was selected as a candidate gene for carcass and meat quality traits in Korean cattle. The objective of this study was to identify single nucleotide polymorphisms (SNPs) in the UCP3 gene and to evaluate the association of UCP3 SNP markers with carcass and meat quality traits in Korean cattle. The five exons in the UCP3 gene were sequenced, and ten SNPs were identified. The PCR-SSCP method was then developed to genotype the individuals examined. The g.3076A>G genotype was significantly associated with marbling score (MS) of Korean cattle. Animals with the AA genotype had a higher MS than those with the AG and GG genotypes. No significant associations of the SNP g.3076A>G were observed for any traits. In conclusion, although SNP g.3076A>G, which showed an association with MS, does not cause amino acid changes, this SNP may be used as a DNA marker to select animals that have higher intramuscular fat content.