• Korean Journal of Food Science and Technology
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• v.44 no.1
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• pp.1-7
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• 2012

There have been few studies related to ELISA for mackerel. In this study we developed a sandwich ELISA for mackerel in processed foods using rabbit polyclonal antibodies against mackerel parvalbumin, the major allergen of mackerel and heat-stable protein. The parvalbumin was purified by ammonium sulfate precipitation and Sephadex G-50 column chromatography. From the standard ELISA curves, the detection limit of parvalbumin was 3 ng/mL and the detection range of mackerel was 5-5,000 ${mu}g/mL$. We further investigated the cross-reactivity of the antibodies toward mackerel, mackerel pike, salmon, flatfish, armorclad rockfish, cod fish, squid, shrimp, blue crab, and lobster. The antibodies were specific for mackerel only. The mean assay recoveries in cooked cream soup, baby food, sausage, and sauce spiked with 0.01 to 0.3% mackerel were 104, 101, 54, and 0%, respectively. In sample tests of 16 commercial items, the qualitative coincidence ratio of assay result and indication was 75%.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.19 no.2
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• pp.118-126
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• 1986

Vibrio vulnificus is a recently recognized halophilic organism that nay cause serious human infections. Patients infected with V. vulnificus often have a history of exposure to the sea, suggesting that the organism may be common inhabitant of marine environment. The purpose of this experiment is to investigate the distribution and bacteriological characteristics of V. vulnificus. The strain used in this experiment was isolated from sea water and sea products such as common octopus (Octopus variabilis), ark shell (Anadara broughtonii), blue crab (Ericheir japonica), and sea squirt (Synthia roretzi) collected in Pusan area from July to October in 1985. V. vulnificus was frequently isolated in August when temperature of sea water was around $26^{\circ}C$ and rarely isolated in October when temperature of sea water was around $18.5^{\circ}C$. The distinctive biochemical characteristics of V. vulnificus were ONPG hydrolysis positive and fermented lactose and not grown in peptone water contained $8\%$ NaCl. The optical density at 660 nm of the growth of V. vulnificus was reached maximum level after 8 hours of culture at $35^{\circ}C$ in brain heart infusion broth but that of V. vulnificus was little increased at $15^{\circ}C$ for 14 hours. Optimum temperature and pH for the growth of V. vulnificus were around $35^{\circ}C$ and 8.0. The specific growth rate and the generation time of V. vulnificus isolated from the samples were $1.21\;hr^{-1}$, 34 min at $35^{\circ}C$ and $0.61\;hr^{-1}$, 69 min at $25^{\circ}C$, respectively. V. vulnificus did not grow on eosin-methylene-blue agar, salmonella-shigella agar, deoxycholate agar but grew well on Endo agar, xylose-lysine-deoxycholate agar and hektoen enteric agar. On Endo agar, the colonies of V. vulnificus were red and achieved a diameter of 2 to 4 mm as a feature enabling differentiation of V. vulnificus from other Vibrio spp. V. vulnificus grow well on TCBS agar forming green colonies. V. vulnificus refrigerated at $4^{\circ}C$ exhibited a linear decline of its viablity as 1 log cycle in every 16 hours storage, while V. vulnificus freezed at $-18^{\circ}C$ almost became extinct.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.39 no.1
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• pp.50-55
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• 2003

In order to study on the improvement of gill nets and trap nets fishing for the management of fisheries resource in the coastal area of Yellow Sea, we have investigated the general present condition of those fishing, and the actual fishing operation of gill nets for croaker and trap nets for rock shell which is called the the other trap nets, and then measured the body length of croaker and blue shell caught by their fishing vessels. The results are as follows ; 1. The total number of permission for coastal fishing are 12,944 cases, but the number of operation for coastal fishing are 7,558 cases in the coastal area of Yellow Sea. Among the total number of permission, the gill nets fishing are 5,154 cases with 39.8% but even so the number of operation are 3,724 cases, the trap nets fishing are 1,025 cases with 7.6% but even so the number of operation are 662 cases. On the fishing ratio, the gill nets and trap nets fishing are comparatively higher than the other fishing with 72.3% and 64.6%, respectively. 2. The main fishing period of gill nets for croaker is from the middle of July to early of September, and the main fishing grounds are sandymud bottom of 15-50m in depth around the islands of southern parts of western coastal area, and the fishing operation carry out 1∼2 times per day in flood tide and nets hauling conduct in 1∼2 hours after drifting with current. 3. The distribution range of body length of 139 croakers, which are caught in the gill nets, are 43.0∼120.0㎝ and the mode is 85.0㎝. 4. The main fishing period of trap nets for rock shell which is called the other trap nets is all the year round except the catching period of blue crab from early of September to the middle of October, and the main fishing ground are the sandymud bottom of 10∼20m in depth, and nets hauling conduct in next day after nets casting. 5. The distribution range of maximum carapace of 5,372 rock shells are 4.5∼8.5㎝ and the mode is 7.5㎝.