• Title/Summary/Keyword: blood chemical

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Studies on the hematological and blood chemical findings for dry and lactating periods (건유(乾乳)와 필유(泌乳)에 따른 유우(乳牛)의 혈액(血液) 및 혈액화학상(血液化學像)의 변화에 관한 연구)

  • Kang, Chung-boo;Lee, Kuk-cheon;Lee, Soon-sun;Cho, Hee-taek;Ahn, Dong-won
    • Korean Journal of Veterinary Research
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    • v.29 no.4
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    • pp.437-443
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    • 1989
  • This experiment was carried out to investigate the hematological and blood chemical values in dairy cattle during the dry and lactating period. Blood was collected from six healthy dairy cattle in Gyeongnam province. Leucocytes count, hemoglobin concentrations and the percentage of packed cell volume were lowest at the early lactation period. Leucocytes count was not affected during dry the to lactating period. The differential count of eosinophilic leucocytes was low at the late lactation period, while the basophilic leucocytes was high at the dry period. Inorganic phosphorus value was below under normal level. Creatinine value was from 1 to 2mg/100ml of serum. Positive reaction to CRP was shown in normal dairy cattle. The values of AST and ALT were higher during the dry than lactating period. r-GTP and total cholesterol tend to be decreased during the lactating period. CPK value was not affected for dry and lactating period.

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Rapid Determination of Volatile Organic Compounds in Human Whole Blood Using Static Headspace Sampling with Gas Chromatography and Mass Spectrometry

  • Lee, Ji-Young;Kim, Seungki;Lee, Jong-Tae;Choi, Jong-Ho;Lee, Jeongae;Pyo, Heesoo
    • Bulletin of the Korean Chemical Society
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    • v.33 no.12
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    • pp.3963-3970
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    • 2012
  • Headspace (HS) and headspace solid-phase microextraction (HS-SPME) were studied for extracting volatile organic compounds (VOCs) from whole blood, with chemical and instrumental variables being optimized for maximum sensitivity: incubation at $60^{\circ}C$, equilibration for 30 min, pH 11, and 2 mL injection volume. Both techniques provided accurate analyses, with detection limits of 0.05-0.1 ng $mL^{-1}$ and 0.05-0.5 ng $mL^{-1}$. HS showed better sensitivity, reproducibility, and analysis times than HS-SPME. Overall levels of chloroform in whole blood were found to be 0.05-5.84 ng $mL^{-1}$; detected levels of benzene were 0.05-2.20 ng $mL^{-1}$.

Mechanisms of Platelet Adhesion on Elastic Polymer Surfaces: Protein Adsorption and Residence Effects

  • Insup Noh;Lee, Jin-Hui
    • Macromolecular Research
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    • v.9 no.4
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    • pp.197-205
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    • 2001
  • Platelet adhesion onto elastic polymeric biomaterials was tested in vitro by perfusing human whole blood at a shear rate of 100 sec$\^$-1/ for possible verification of mechanisms of initial platelet adhesion perfusion of blood on the polymeric substrates was performed after treatments either with or without pre-adsorption of 1% blood plasma, and either with or without residence of the protein-preadsorbed substrate in phosphate buffered solution. The surfaces employed were elastic polymers such as poly(ether urethane urea), poly(ether urethane), silicone urethane copolymer, silicone rubber and poly(ether urethane) with the anti-calcifying agent hydroxyethane bisphosphate. Each polymer surface treated was exposed in vitro to the dynamic, heparinized whole blood perfused for upto 6 min and the surface area of platelets initially adhered was measured by employing in situ epifluorescence video microscopy. The blood perfusion was performed on the surfaces treated at the following three different conditions: directly on the bare surfaces, after protein pre-adsorption and after residence in buffer for 3 days of the surfaces protein pre-adsorbed for 2 h. The effects of blood plasma pre-adsorption on the initial platelet adhesion was surface-dependent. The amount of the adsorbed fibrinogen and the surface coverage area of the adhered platelets were dependent on the surface conditions whether substrates were bare surfaces or protein pre-adsorbed ones. To test an effect of possible morphological (re)orientations of the adsorbed proteins on the initial platelet adhesion, the polymeric substrate pre-adsorbed with 1% blood plasma was immersed in phosphate buffered solution for 3 days and then exposed to physiological blood perfusion. The surface area of the platelets adhered on these surfaces was significantly different from that of the surfaces treated with protein pre-adsorption only. These results indicated that platelet adhesion was dependent on the surface property itself and pre-treatment conditions such as blood perfusion without any pre-adsorption of proteins, and blood perfusion either after protein pre-adsorption or after subsequent substrate residence in buffer of the substrate pre-adsorbed with proteins. Understanding of these results may guide for better designs of blood-contacting materials based on protein behaviors.

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Studies on the Blood Chemical Values in Thoroughbred Foals (Thoroughbred종 망아지의 혈액화학식에 관한 연구)

  • 이미숙;최귀철;이경갑
    • Journal of Veterinary Clinics
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    • v.17 no.2
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    • pp.334-339
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    • 2000
  • The considerable researches have been done on the change,1 associated with age in the blood picture of domestic animals, but a survey has not been of the blood picture in Thoroughbred fears in Korea. Therefore, the purpose of this study is to outline the range of values in clinically not\\\\`- mal Thoroughbred foals. Some blood chemical parameters from 3 days to 8 weeks of age were exam-ined to obtain normal values in Thoroughbred foal7. Blood samples were obtained from the jugular vein in 11 Thoroughbred foals and collected at 3 days, and at 1,2,4 and 8 weeks of age, respectively. The results obtained ill this study were summarized as follows: The packed cell volume (PCV) at 4 weeks of age was significantly lower than that at 3 days of age (P<0.01) and that al 2 and 8 weeks of age were straightly lower than that at 3 days of age (P<0.05). The total protein (TP) at 8 weeks of age was higher than that at 3 days, 1 and 2 weeks of age (P<0.05). The activity of aspartate ami-notransferase (AST) at 2,4 and 8 weeks of age were significantly higher than that at 3 days of age (P<0.01). The total bilirnbin showed a tendency to decrease gradually according to the weeks and that at 2,4 and 8 weeks of age were significantly lower than that at 3 days of age (P<0.01). The BUN at 1 and 8 weeks of ago were lower than that at 3 days of age (P<0.05). The concentration of Ca at 1 week of alee was higher than that at 3 days of age (P<0.05). The vitamin A and vitamin E showed a tendency to decrease gradually from 3 days to 4 weeks of age and increased slightly at 8 weeks of age.

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In vitro investigation of algin impregnated vascular graft (알진이 도포된 인공혈관의 물성 평가)

  • Lee, Jin-Ho;Shin, Bung-Chul;Khang, Gil-Son;Lee, Hai-Bang
    • Proceedings of the KOSOMBE Conference
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    • v.1990 no.05
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    • pp.9-12
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    • 1990
  • He impregnated a highly porous, knitted polyester (Dacron) graft with a biodegradable nonproteinaceous material, algin. This new vascular graft is blood tight but still retains high porosity in the body. It does not need to be preclotted with blood before implantation and has good tissue ingrowth and biological healing properties due to the high porosity. The algin impregnated graft was investigated by "in vitro" examinations in this study. It was characterized by ESCA analysis, SEM observation, and measurements of water permeability, algin coating weight, mechanical properties and whole blood clotting time. The water permeability of the graft was reduced more than 99% by the algin impregnation treatment without changing any mechanical properties. "In vivo" examinations of the algin impregnated vascular graft are on progress.

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Effects of rBST, Vitamin E and Selenium Administration on Semen Characteristics, Blood Chemical Values and Hormone in Hanwoo Sires III. Effects of rBST, Vitamin E and Selenium Administration on Blood Chemical Values and Hormone Concentration of Blood in Hanwoo Sires (rBST, Vitamin E 및 Selenium 투여가 한우 종모우의 정액성상, 혈액성분 및 호르몬 변화에 미치는 효과 III. rBST, Vitamin E 및 Selenium 투여가 한우 종모우의 혈액성분 및 호르몬 농도 변화에 미치는 효과)

  • 박동헌;양부근;김종복;정희태;박춘근;김정익;이성수;박노형;원유석
    • Korean Journal of Animal Reproduction
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    • v.24 no.2
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    • pp.163-170
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    • 2000
  • This study was conducted to examine the effects of recombinant Bovine Somatotropin (rBST), vitamin E(Vit. E) and selenium (Se) administration on the blood chemical values and hormone concentrations of serum in Hanwoo sires. Hanwoo sires were randomly assigned to five groups; 1) control, 2) rBST,0.09mg/kg body weight (BW), 3) Vit E, 1,500IU/kg BW, 4) Se 0.1mg/kg BW, 5) Vit E, 1,500IU plus Se 0.1mg/kg BW. rBST, Vit. E and Se for each experimental group were given 6 times at 15 days interval by intermuscular injection. Blood samples were collected ten times for experimental periods and separated the serum by centrifugation and stored into deep freezer. The concentration of albumin was the highest in Se group than those of any other groups (P<0.05) and Vit. E plus Se group was significantly higher than in the control and rBST groups (P<0.05). The concentrations of blood urea nitrogen (BUN) and creatinine were significantly higher in rBST group than any other groups (P<0.05). The concentration of total protein in rBST, Se and Vit. E plus Se groups were significantly higher than in control group (P<0.05). The concentrations of calcium, cholesterol,, glucose, inorganic phosphorus and triglycerides in serum were not difference in all experimental groups (P>0.05). The concentration of estradiol was slightly higher in Se and Vit. E plus Se groups than those of any other groups, but not significantly difference among the experimental groups (P<0.05). The concentration of testosterone was not affected by the administration of rBST, Vit. E and Se.

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Survey on Blood Picture and Its Chemical Value to Decision of Nutritional Level of Dairy Cattle (젖소의 영양판정을 위한 혈액상 및 혈액화학치 조사)

  • Paik Young-Ki;Lee Joo-Muk;Kim Jin-Ku
    • Journal of the korean veterinary medical association
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    • v.16 no.1112
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    • pp.349-353
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    • 1980
  • To make a survey on present nutritioal level of dairy cattle (Holstein) which are fed in Jeonbug Province, The examination of their blood and chemical values were carried out. The results summarized were as follows: 1. WBC counts were $8.49{\pm}1.73(

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Blood Chemical Values of Fascioliasis in Holstein Cow (간질에 감염된 젖소의 혈액화학치에 관한 연구)

  • Kim Tae-Jong;Lee Won-Chang;Kim Hak-Jae;Song Kee-Hoe
    • Journal of the korean veterinary medical association
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    • v.26 no.4
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    • pp.226-231
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    • 1990
  • In order to investigate the infectious rate of the internal parasites, A total of nine hundred and five head of Holstein cow in the area of Kyungi Do was randomly selected for the fecal examination. And the chemical values of blood were examined from 20 H

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Somatic Cell Counts and Blood Chemical Values of the Bovine Mastitis (유방염 유우의 체세포수 측정과 혈액화학치에 관한 연구)

  • Lee Ji-Sub;Kim Tae-Jong;Yoon Chong-Sam
    • Journal of the korean veterinary medical association
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    • v.23 no.10
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    • pp.663-670
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    • 1987
  • In order to investigate the rate of clinical mastitis and the average number of somatic cell in a milk, the number of somatic cell in the milk counted from 82 normal Holstein cows in the area of Kyungi-Do. And the chemical values of blood were examined fr

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