• Title/Summary/Keyword: blocking temperature

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Regulation of Chlorophyll-Protein Complex Formation and Assembly in Wheat Thylakoid Membrane

  • Guseinova, I.M.;Suleimanov, S.Y.;Aliev, J.A.
    • BMB Reports
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    • v.34 no.6
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    • pp.496-501
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    • 2001
  • Lincomycin, an inhibitor of plastid protein synthesis, was found to block the synthesis of apoprotein P700 with a molecular mass of 72 kDa and the assembly of the Chl a-protein of PS I. Synthesis of the polypeptides of 48, 43.5, and 32 kDa of the PS II complex is also suppressed. This process is accompanied by the disappearance of the PS Two reaction center Chl a at 683 nm, and of the PS One reaction center Chl a at 690, 696, and 705 nm on the fourth derivative of the absorption spectra at 77K. Lincomycin does not affect the synthesis of LHC subunits. It increases the content of the two main Chl forms of LHC at 648 nm (Chl b) and 676 nm (Chl a). The low-temperature fluorescence ratio F736/F685 is also increased. However, the effect of cycloheximide (an inhibitor of cytoplasmic protein synthesis) leads to the reduction of polypeptides of the light-harvesting Chl a/b-protein complex in the range of 29.5-22 kDa. Under these conditions, the relative amount of Chl b and the F736/ F685 fluorescence ratio decrease significantly. This is obviously the result of blocking the LHC I and LHC II synthesis. At the same time rifampicin and actinomycin D (inhibitors which block transcription in chloroplast and nuclear genome, respectively) inessentially affect the characteristics of these complexes.

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Design of Electrical equivalent circuit of Planar Buried Heterostructure Laser Diode (평면 매립형 레이저 다이오드의 전기적 등가회로 모델)

  • Kim Jeong-Ho;Park Dong-Kook
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.10 no.4
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    • pp.718-723
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    • 2006
  • Optical module plays an important role in the construction of high speed communication network. Laser diode is a component of optical module, and its characteristics are dependent of temperature, so many researches are reported. In this paper, we proposed the electrical equivalent circuit of PBH-LD based on the rate equations. And, the two leakage paths exit outside the active region. One path is converted pn-diode and the other path is converted two transistors using npn-Tr and pnp-Tr. In order to reduce the leakage currents, we observed the dependence of carrier concentrations of current blocking layers using PSPICE simulator.

Effects of Experimental Variables on the Measurement $T_{cv}$ of Crystalline slags (결정슬래그의 $T_{cv}$ 측정 시 실험변수에 따른 영향)

  • Kim, Yu-Na;Oh, Myong-Sook S.
    • 한국신재생에너지학회:학술대회논문집
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    • 2008.10a
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    • pp.254-257
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    • 2008
  • For crystalline slags, of which the viscosity rapidly increases at $T_{cv}$ due to the formation of crystalline phases, the Tcv is affected by measurement conditions. In this study, we investigated the effect of cooling rate, and alumina dissolution on the determination of $T_{cv}$. Using synthetic slag samples based on the composition of Alaska Usibelli slag, $T_{cv}$ were determined under a constant cooling rate of $2^{\circ}C$/min, and under rapid cooling with holding time to allow the slag to reach thermal and rheological equilibrium. The effect of alumina dissolution was investigated using platinum lined crucibles. The constant cooling resulted in lower $T_{cv}$ by $33^{\circ}C$ as compared to the equilibrium measurements. Under $2^{\circ}C$/min cooling, the blocking alumina dissolution resulted in lower $T_{cv}$ by $23^{\circ}C$. When the $T_{cv}$ was measured under $2^{\circ}C$/min cooling using an alumina crucible, therefore, the effects of a constant cooling is somewhat offset by the alumina dissolution effect, and bring the measured value closer to the true value.

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Differential Regulation of the Genes of the Streptococcus pneumoniae dnaK Operon by Ca++

  • Kim, Seung-Whan;Bae, Yong-Goo;Pyo, Suhk-Neung;Rhee, Dong-Kwon
    • Molecules and Cells
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    • v.23 no.2
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    • pp.239-245
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    • 2007
  • DnaK is a major antigen in Streptococcus pneumoniae, and is induced by a minor shift in temperature (30 to $37^{\circ}C$) but not by ethanol shock. Although HrcA in the presence of $Ca^{{+}{+}}$ represses the expression of both groEL and hrcA, the control of transcription of the dnaK operon is not completely understood. In this study, the dnaK operon of S. pneumoniae (5' hrcA-grpE-dnaK-dnaJ) was cloned and analyzed. It contains large intergenic regions in grpE/dnaK and dnaK/dnaJ. Pulse labeling with [$^{35}S$]-methionine and immunoblot analyses revealed the presence of higher levels of DnaK than of HrcA even in the presence of $Ca^{{+}{+}}$ after heat shock suggesting that $Ca^{{+}{+}}$ differentially regulates the heat shock responses of hrcA and dnaK. By blocking de novo mRNA synthesis with rifampin it was shown that neither the hrcA nor the groEL transcripts were stabilized by heat shock even though dnaK transcripts were stabilized. We conclude that S. pneumoniae uses fine regulation of the transcription of the individual genes of the tetracistronic dnaK operon to cope with the various stresses experienced during infections.

A Study on Block from Spread of Fire of the Exteriors Installation Space (외장재 설치 공간의 화재확산 차단에 관한 연구)

  • Min, Se-Hong;Yun, Jung-Eun;Kim, Mi-Suk
    • Journal of the Korea Safety Management & Science
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    • v.14 no.2
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    • pp.83-89
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    • 2012
  • In this study, FDS fire simulation experiments and measured wind speed by applying the exterior installation portion for blocking the spread of the fire was investigated. As a result, aluminum composite panels installed in the lower and the upper part of the panel to remove all the lower side, and then the maximum wind speed 0.24 m/s and the upper side 0.58 m/s were measured. In the FDS, the measured wind speed difference air currents are approximately 3.7 times in 12 seconds, the occurrence of 17 seconds early moment wind 2.2 m/s was measured from. Before and after the fire occurred in early of the air velocity about 39 seconds was 3.5 times difference. Such air currents caused by the temperature of the building but also by the building height was found. Turbulent flame of fire by expanding the vertical extent of damage become greatly important factor. Therefore, through the exterior installation portion of the block that can delay the spread of fire is expected that this should be taken.

Microstructure and Thermal Properties of Mn-Ir/Ni-Ee Exchange Biased Multilayers (Mn-Ir/Ni-Fe 교환결합형 다층박막의 미세구조 및 열적특성)

  • 윤성용;전동민;김장현;서수정;노재철;이확주
    • Journal of the Korean Magnetics Society
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    • v.10 no.6
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    • pp.274-279
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    • 2000
  • The microstructure and thermal properties of the Mn-IriNi-Fe exchange biased multi-layers with various buffer layers and stacking structures have been investigated. The H$_{e{\chi}}$ and the T$_{b}$ depend on the Mn-Ir grain size at the interface between the Mn-Ir layer and the Ni-Fe layer, The (111) preferred orientation of Mn-Ir/Ni-Fe on the Ta buffer layer may promote the values of J$_{k}$ and H$_{e{\chi}}$. The samples which produce the Hex have the epitaxial relationship at the interface between the Mn-Ir layer and the Ni-Fe layer due to the generation of misfit dislocation.

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Resistance Spot Welding Characteristics of Mg Alloy Applying Current Waveform Control (전류 파형 제어를 적용한 마그네슘 합금의 저항 점 용접 특성)

  • Choi, Dong-Soon;Hwang, In-Sung;Kim, Dong-Cheol;Ryu, Jae-Wook;Kang, Moon-Jin
    • Journal of Welding and Joining
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    • v.32 no.2
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    • pp.70-75
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    • 2014
  • In automotive industry, applying of Mg alloy to autobody has been issued recently as a light metal. But poor resistance spot weldability of Mg alloy is blocking commercialization. So studies on improving resistance spot weldability of Mg alloy is increasing continuously. For reduce loss of heat input during welding, inverter DC power source is considered because of short rise time to target welding current. But rapid rising of welding current can increase temperature rapidly in nugget and oxide film between electrode and base metal, and that causes generating expulsion on low welding current range. In this study, for increase optimum welding current range and prevent generating expulsion, applicate various types of welding current waveform controls during resistance spot welding. For analysis effects of each current waveform control, acceptable welding current regions according to electrode force and welding time is determined and lobe diagram is derived. In result, pre heat is proposed as optimum type of welding current waveform control.

Characterization of Pervaporation Membranes and Its Operation Parameters for Alcohol Concentration (알코올 농축을 위한 투과증발막의 특성 및 조업변수 연구)

  • 임군택;김현일;김성수
    • Membrane Journal
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    • v.9 no.2
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    • pp.107-113
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    • 1999
  • Alcohol concentration via pervaporation process was performed by using commercial PDMS(polydimethylsiloxane) composite membrane and plasma treated PP(polypropylene) membranes. Effects of operating parameters of pcrvaporation process were examined. With the increase of butanol concentration in the feed, flux and selectivity increased due to the greater affinity of butanol with PDMS than that of water. As the operating temperature increased, free volume as well as the solubilities and diffusivities of alcohol and water increased to result in the greater flux and less selectivity of the membrane. As solubility parameter difference between alcohol and PDMS membrane decreased, high flux and good selectivity were obtained. When PP membrane was plasma treated with methanol, it has 6 times greater flux than PDMS membrane with equivalent separation factor. With the increase of plasma treatment time, flux and selectivity were enhanced. However, excess treatment time caused pore blocking to result in the flux decrease.

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Performance Analysis of Summertime Heat Transfer Characteristics of the Double Skin Window for Plant Factory (식물공장 이중창호의 하절기 열전달 성능 분석)

  • So, Jae-Hyun;Kim, Woo-Tae
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.24 no.4
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    • pp.351-357
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    • 2012
  • To reduce the summertime cooling load of a plant factory, a concept design was performed for the double skin window which utilizes the low temperature air from a ground coupled heat exchanger. The design parameters were selected as the number of cavity air inlet, the cavity thickness, the location of cavity air inlet, and the configuration of cavity air outlet. A parametric study was conducted in a systematic way to evaluate the heat transfer characteristics of the double skin window. As the number of cavity air inlet and the cavity thickness increase, the heat flux from outside air to indoor air was decreased. The effect of the location of cavity air inlet was not significant and the larger cavity air outlet area gave us relatively better heat blocking performance from outside hot air. This study demonstrated that it is possible to develop an improved double skin window by utilizing a ground coupled heat exchanger.

A Field Application Case of Direct Ice Slurry Transporting System for District Cooling (지역냉방용 직접순환식 아이스슬러리 시스템의 현장적용 사례)

  • Yoo, Ho-Seon;Lee, Sang-Hoon;Lee, Yoon-Pyo
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.21 no.9
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    • pp.496-504
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    • 2009
  • In order to investigate the feasibility of a direct ice slurry transporting system for the purpose of district cooling, a case study of field application is performed. The research aims include the field measurement of ice packing factor, the performance of coldness delivery, and the branching characteristics of ice slurry. Two representative types of pipe branch are dealt with in this work. For the slurry flow with ice volume fraction of 0.16 or less, the pipe blocking due to aggregation is not observed. Based on the time-wise variation of temperature in the storage tank, a calculating method of ice packing factor is newly developed, which seems to be useful when the brine concentration is unknown. It is confirmed that the mass flow rate of ice slurry per unit cooling load is markedly reduced with increasing the ice content. The pumping power also decreases, but remains unchanged for high ice fractions. The distribution of ice particle before and after branching shows a good uniformity within the range of 5% difference, but yields a unique trend depending on the flow rate.