• Title/Summary/Keyword: blocking temperature

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Development of a Denaturing High-Performance Liquid Chromatography (DHPLC) Assay to Detect Parasite Infection in Grass Shrimp Palaemonetes pugio

  • Cho, Sang-Man
    • Fisheries and Aquatic Sciences
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    • v.15 no.2
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    • pp.107-115
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    • 2012
  • In developing a useful tool to detect parasitic dynamics in an estuarine ecosystem, a denaturing high-performance liquid chromatography (DHPLC) assay was optimized by cloning plasmid DNA from the grass shrimp Palaemonetes pugio, and its two parasites, the trematode Microphallus turgidus and bopyrid isopod Probopyrus pandalicola. The optimal separation condition was an oven temperature of $57.9^{\circ}C$ and 62-68% of buffer B gradient at a flow rate of 0.45 mL/min. A peptide nucleic acid blocking probe was designed to clamp the amplification of the host gene, which increased the amplification efficiency of genes with low copy numbers. Using the DHPLC assay with wild-type genomic, the assay could detect GC Gram positive bacteria and the bopyrid isopod (P. pandalicola). Therefore, the DHPLC assay is an effective tool for surveying parasitic dynamics in an estuarine ecosystem.

Electrical Relaxation in Silica Glasses and Nonlinearity in Electrical Conductivity (실리카 유리의 전기이완 특성과 비선형적 전기전도도)

  • 신동욱
    • Journal of the Korean Ceramic Society
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    • v.36 no.9
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    • pp.923-929
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    • 1999
  • The cause of optical nonlinearity induced in thermally poled silica glass is believed to be the space charge polarization. Since the second order optical nonlinearity (electro-optic effect) can be used in optical switches the optical nonlinearity in silica glass has drawn a large attention. Space charge polarization occurs when an ionic conducting material is subjected to dc electric field by the blocking electrode. Thermal poling performed to induce the optical nonlinearity in silica glass is basically identical to the process generating space charge polarization. As a first step to understand the mechanism of space charge polarization in silica glass hence the induced optical nonlinearity the absorption currents as functions of time were measured for various types of silica glasses and analyzed by the theory of space charge polarization. It was found that the electrical relaxation exhibited a step by the space charge polarization in the relatively long time range and dielectric loss peak showed a maximum at a specific temperature which is depending on type of silica glass. It was turned out that this relaxation might be a cause of nonlinearity in electrical conductivity of silica glass.

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Study on Synthesis and Magnetic Properties of Cobalt Nanoparticles in the Polymer Film (코발트 나노 입자가 도입된 고분자 박막의 제조 및 자성 연구)

  • 박일우;윤명근;김유경;김영미;김종현;전미선;조용민;김상우
    • Proceedings of the Korean Magnestics Society Conference
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    • 2003.06a
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    • pp.136-137
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    • 2003
  • 고분자 재료인 이온교환수지 박막 안에서의 이온교환반응과 전기화학적 환원반응을 이용하여 코발트 나노 입자를 제조하였다. 투과전자현미경 결과로부터 고분자 박막 (MF-4SK) 1 gram에 코발트가 7.8$\times$$10^{19}$ atoms 포함된 시편에서 코발트가 나노 크기로 입자를 형성하고 있음을 확인하였으며, 자기측정 결과로부터 코발트 나노 입자가 blocking temperature (T$_{B}$) 이상에서 초상자성을 나타내는 것을 확인하였다. 이 결과는 고분자 박막 내에서 코발트 나노 입자가 자성 단상(single domain) 구조를 이루고 있음을 보여주는 것으로, 강자성 나노 입자들의 초상자성 거동을 고찰하였다.

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Hydrophilic Modification of Polypropylene Hollow Fiber Membrane by Dip Coating, UV Irradiation and Plasma Treatment

  • Kim Hyun-Il;Kim Jin Ho;Kim Sung Soo
    • Korean Membrane Journal
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    • v.7 no.1
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    • pp.19-27
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    • 2005
  • PP hollow fiber membrane was hydrophilized by EVOH dip coating followed by low temperature plasma treatment and UV irradiation. EVOH coating attained high water flux without any prewetting but its stability did not guaranteed at high water permeation rate. At high water permeation rate, water flux declined gradually due to swelling and delamination of the EVOH coating layer causing pore blocking effect. However, plasma treatment reduces the swelling, which suppress delamination of the EVOH coating layer from PP support result in relieving the flux decline. Also, UV irradiation helped the crosslinking of the EVOH coating layer to enhance the performance at low water permeation rate. FT-IR and ESCA analyses reveal that EVOH dip coating performed homogeneously through not only membrane surface but also matrix. Thermogram of EVOH film modified plasma treatment and W irradiation show that crosslinking density of EVOH layer increased. Chemical modification by plasma treatment and UV irradiation stabilized the hydrophilic coating layer to increase the critical flux of the submerged membrane.

Efficient Organic Light-Emitting Diodes with a use of Hole-injection Buffer Layer

  • Kim, Sang-Keol;Chung, Dong-Hoe;Chung, Taek-Gyun;Kim, Tae-Wan
    • 한국정보디스플레이학회:학술대회논문집
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    • 2002.08a
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    • pp.766-769
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    • 2002
  • We have seen the effects of hole-injection buffer layer in organic light-emitting diodes using copper phthalocyanine(CuPc), poly(vinylcarbazole)(PVK), and Poly(3,4-ethylenedioxythiophene):poly(styrene-sulfonate)(PEDOT:PSS) in a device structure of ITO/buffer/TPD/$Alq_3$/Al. Polymer PVK and PEDOT:PSS buffer layer was made using spin casting method and the CuPc layer was made using thermal evaporation. Current-voltage characteristics, luminance-voltage characteristics and efficiency of device were measured at room temperature with a thickness variation of buffer layer. We have obtained an improvement of the external quantum efficiency by a factor of two, four, and two and half when the CuPc, PVK, and PEDOT:PSS buffer layer are used, respectively. The enhancement of the efficiency is attributed to the improved balance of holes and elelctrons due to the use of hole-injection buffer layer. The CuPc and PEDOT:PSS layer functions as a hole-injection supporter and the PVK layer as a hole-blocking one.

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Investigation on the cold start characteristics of PEMFC using Axiomatic Design approach (Axiomatic Design 기법을 이용한 연료전지 냉시동 특성 개선에 관한 연구)

  • Suh, Jung-Do;Lee, Sung-Ho;Ahn, Byung-Ki;Lim, Tae-Won;Yu, Ha-Na;Lee, Dai-Gil
    • 한국신재생에너지학회:학술대회논문집
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    • 2008.10a
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    • pp.17-20
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    • 2008
  • Cold start of a fuel cell system is a major obstacle should be overcome as to commercialize it, especially for passenger vehicle applications. However, the cold start characteristics is so complicated since it involves various phenomenon such as ice-blocking in GDL, ionic conductivity in membrane affected by water activity with phase change, heat transfer through components such as bipolarplates or endplates, electro-chemical reactions affected by circumferential temperature and humidity as well. Axiomatic design provides a systematic method to investigate the complex phenomenon although it was developed as a methodology to establish logical design procedure by Nam P. Suh in 1990s. This paper presents a framework to approach the complex cold start problem using Axiomatic Design which features simplifying a problem through hierarchical decomposition and decoupling from the view of functional requirements and design parameters.

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Induced Second Order Optical Nonlinearity in Thermally Poled Silica Glasses (Poling된 실리카 유리의 2차비선형광학효과와 공간전하분극의 관계)

  • 신동욱
    • Journal of the Korean Ceramic Society
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    • v.36 no.12
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    • pp.1374-1380
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    • 1999
  • The cause of Scond Harmonic Generation (SHG) in thermally poled silica glass is suggested basedon the electrical and dielectric relaxation measurements. The absorption currents as functions of time were measured for various types of silica glasses and analyzed by the theory of Space Charge Polarization. Space charge polarization occurs when an ionic conducting material is subjected to dc electric field with blocking electrode. Thermal poling performed to induce SHG in silica glass is basically identical to the process generating space charge polarization. Hence it was found that gene-ration removal reproduction and temperature dependence of SHG in poled silica is directly related to those of space charge polarization. It turned out that the fundamental parameters governing the SHG in poled silica are charge carrier concentration and mobility. Based on the theory of space charge polarization and experimental results of electrical rela-xation the method to increase the intensity of SHG is proposed.

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Inhibiting Effect of Nicotinic Acid Hydrazide on Corrosion of Aluminum and Mild Steel in Acidic Medium

  • Bhat, J. Ishwara;Alva, Vijaya D.P.
    • Journal of the Korean Chemical Society
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    • v.58 no.1
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    • pp.85-91
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    • 2014
  • The corrosion behavior of aluminum and mild steel in hydrochloric acid medium was studied using a nicotinic acid hydrazide as inhibitor by potentiodynamic polarization, electrochemical impedance spectroscopy technique and gravimetric methods. The effects of inhibitor concentration and temperature were investigated. The experimental results suggested, nicotinic acid hydrazide is a good corrosion inhibitor for both aluminum and mild steel in hydrochloric acid medium and the inhibition efficiency increased with increase in the inhibitor concentration. The polarization studies revealed that nicotinic acid hydrazide exhibits mixed type of inhibition. The inhibition was assumed to occur via adsorption of the inhibitor molecules on the aluminum and mild steel surface and inhibits corrosion by blocking the reaction sites on the surface of aluminum.

Identification of Inhibitors Against BAK Pore Formation using an Improved in vitro Assay System

  • Song, Seong-Soo;Lee, Won-Kyu;Aluvila, Sreevidya;Oh, Kyoung Joon;Yu, Yeon Gyu
    • Bulletin of the Korean Chemical Society
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    • v.35 no.2
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    • pp.419-424
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    • 2014
  • The pro-apoptotic BCL-2 family protein BID activates BAK and/or BAX, which form oligomeric pores in the mitochondrial outer membrane. This results in the release of cytochrome c into the cytoplasm, initiating the apoptotic cascade. Here, we utilized liposomes encapsulating sulfo-rhodamine at a controlled temperature to improve upon a previously reported assay system with enhanced sensitivity and specificity for measuring membrane permeabilization by BID-dependent BAK activation. BAK activation was inhibited by BCL-$X_L$ protein but not by a mutant protein with impaired anti-apoptotic activity. With the assay system, we screened a chemical library and identified several compounds including trifluoperazine, a mitochondrial apoptosis-induced channel blocker. It inhibited BAK activation by direct binding to BAK and blocking the oligomerization of BAK.

Liquid Phase Deposition of Transition Metal Ferrite Thin Films: Synthesis and Magnetic Properties

  • Caruntu Gabriel;O'Connor Charles J.
    • Journal of the Korean Ceramic Society
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    • v.43 no.11 s.294
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    • pp.703-709
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    • 2006
  • We report on the synthesis of highly uniform, single phase zinc and cobalt thin films prepared by the Liquid Phase Deposition (LPD) method. X-Ray diffraction, TGA and EDX measurements support the assumption that the as deposited films are constituted by a mixture of crystallized FeOOH and amorphous M(OH)$_2$ (M=Co, Zn) which is converted upon heat treatment in air at 600?C into the corresponding zinc ferrites. The films with adjustable chemical compositions are identified with a crystal structure as spinel-type and present a spherical or rod-like microstructure, depending on the both the nature and concentration of the divalent transition metal ions. Zinc ferrite thin films present a superparamagnetic behavior above blocking temperatures which decrease with increasing the Zn content and are ferromagnetic at 5 K with coercivities ranging between 797.8 and 948.5 Oe, whereas the cobalt ferrite films are ferromagnetic at room temperature with magnetic characteristics strongly dependent on the chemical composition.