• Molecules and Cells
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• v.25 no.3
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• pp.407-416
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• 2008

Thirteen near-isogenic lines (NILs) of japonica rice were developed via a backcross method using the recurrent parent Chucheong, which is of good eating quality but is susceptible to Magnaporthe grisea, and three blast resistant japonica donors, Seolak, Daeseong and Bongkwang. The agro-morphological traits of these NILs, such as heading date, culm length, and panicle length, were similar to those of Chucheong. In a genome-wide scan using 158 SSR markers, chromosome segments of Chucheong were identified in most polymorphic regions of the 13 NIL plants, and only a few chromosome segments were found to have been substituted by donor alleles. The genetic similarities of the 13 NILs to the recurrent parent Chucheong averaged 0.961, with a range of 0.932-0.984. Analysis of 13 major blast resistance (R) genes in these lines using specific DNA markers showed that each NIL appeared to contain some combination of the four R genes, Pib, Pii, Pik-m and Pita-2, with the first three genes being present in each line. Screening of nine M. grisea isolates revealed that one NIL M7 was resistant to all nine isolates; the remaining NILs were each resistant to between three and seven isolates, except for NIL M106, which was resistant to only two isolates. In a blast nursery experiment, all the NILs proved to be more resistant than Chucheong. These newly developed NILs have potential as commercial rice varieties because of their increased resistance to M. grisea combined with the desirable agronomic traits of Chucheong. They also provide material for studying the genetic basis of blast resistance.

• Molecules and Cells
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• v.42 no.9
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• pp.637-645
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• 2019

Effector-triggered immunity (ETI) is an effective layer of plant defense initiated upon recognition of avirulence (Avr) effectors from pathogens by cognate plant disease resistance (R) proteins. In rice, a large number of R genes have been characterized from various cultivars and have greatly contributed to breeding programs to improve resistance against the rice blast pathogen Magnaporthe oryzae. The extreme diversity of R gene repertoires is thought to be a result of co-evolutionary history between rice and its pathogens including M. oryzae. Here we show that Pii is an allele of Pi5 by DNA sequence characterization and complementation analysis. Pii-1 and Pii-2 cDNAs were cloned by reverse transcription polymerase chain reaction from the Pii-carrying cultivar Fujisaka5. The complementation test in susceptible rice cultivar Dongjin demonstrated that the rice blast resistance mediated by Pii, similar to Pi5, requires the presence of two nucleotide-binding leucine-rich repeat genes, Pii-1 and Pii-2. Consistent with our hypothesis that Pi5 and Pii are functionally indistinguishable, the replacement of Pii-1 by Pi5-1 and Pii-2 by Pi5-2, respectively, does not change the level of disease resistance to M. oryzae carrying AVR-Pii. Surprisingly, Exo70F3, required for Pii-mediated resistance, is dispensable for Pi5-mediated resistance. Based on our results, despite similarities observed between Pi5 and Pii, we hypothesize that Pi5 and Pii pairs require partially distinct mechanisms to function.

• Plant Breeding and Biotechnology
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• v.7 no.3
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• pp.272-286
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• 2019

Developing elite hybrid rice varieties is one important objective of rice breeding programs. Several genes related to male sterilities, restores, and pollinators have been identified through map-based gene cloning within natural variations of rice. These identified genes are good targets for introducing genetic traits in molecular breeding. This study was conducted to breed elite hybrid lines with major genes related to hybrid traits and disease/insect resistance in 240 genetic resources and F₁ hybrid combinations of rice. Molecular markers were reset for three major hybrid genes (S5, Rf3, Rf4) and thirteen disease/insect resistant genes (rice bacterial blight resistance genes Xa3, Xa4, xa5, Xa7, xa13, Xa21; blast resistance genes Pita, Pib, Pi5, Pii; brown planthopper resistant genes Bph18(t) and tungro virus resistance gene tsv1). Genotypes were then analyzed using molecular marker-assisted selection (MAS). Biological assay was then performed at the Red River Delta region in Vietnam using eleven F₁ hybrid combinations and two control vatieties. Results showed that nine F₁ hybrid combinations were highly resistant to rice bacterial blight and blast. Finally, eight F₁ hybrid rice varieties with resistance to disease/insect were selected from eleven F₁ hybrid combinations. Their characteristics such as agricultural traits and yields were then investigated. These F₁ hybrid rice varieties developed with major genes related to hybrid traits and disease/insect resistant genes could be useful for hybrid breeding programs to achieve high yield with biotic and abiotic resistance.

• Korean Journal of Computational Design and Engineering
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• v.20 no.4
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• pp.367-374
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• 2015

Sandwich panels consisting of various materials have widely been applied for mitigating dynamic impacts such as ballistic and blast impacts. Especially, the selection of materials for different core set-ups can directly influence its performance. In this study, we design the sandwich panels for alleviating ballistic and blast impacts by controlling the stacking sequence of core materials and their thicknesses. FEM studies are performed to simulate the dynamic behavior of sandwich panels subjected to ballistic and blast impacts. Delamination between the core layers is also considered in the FEM studies for feasible design. Based on the FEM data, kriging models are generated for approximating design space and quickly predicting the FEM outputs. Finally, design optimizations are implemented to find the optimum stacking sequence of core materials and thicknesses with given impact situations.

• Earthquakes and Structures
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• v.21 no.5
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• pp.461-475
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• 2021

Strategic structures are a potential target of the growing terrorist attacks, so their performance under explosion hazard has been paid attention by researchers in the last years. In this regard, the aim of this study is to evaluate the blast-resistance performance of lead-rubber bearing (LRB) base isolation system based on a probabilistic framework while uncertainties related to the charge weight and standoff distance have been taken into account. A sensitivity analysis is first performed to show the effect of explosion uncertainty on the response of base-isolated buildings. The blast fragility curve is then developed for three base-isolated steel moment-resisting buildings with different heights of 4, 8 and 12 stories. The results of sensitivity analysis show that although LRB has the capability of reducing the peak response of buildings under explosion hazard, this control system may lead to increase in the peak response of buildings under some explosion scenarios. This shows the high importance of probabilistic-based assessment of isolated structures under explosion hazard. The blast fragility analysis shows effective performance of LRB in mitigating the probability of failure of buildings. Therefore, LRB can be introduced as effective control system for the protection of buildings from explosion hazard regarding uncertainty effect.

• Proceedings of the Korea Concrete Institute Conference
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• 2006.05b
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• pp.329-332
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• 2006

This paper presents the experimental results for durability of an ECC designed with ground granulated blast furnace slag (BFS) through the test method of chloride ion resistance and freezing-thawing resistance. In order to compare with ECC, normal mortar was also tested. Test results showed that BFS ECC exhibited higher durability performance than ordinary mortar. These results suggest that by adding BFS in ECC, its matrix density is increased which results in decreased of deterioration and it also adds to the fiber bridging that contributes in control of cracking.

• Proceedings of the Korea Concrete Institute Conference
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• 1993.10a
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• pp.39-44
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• 1993

The aim of this study is to develop High Performance Concrete, which can fill in every corner of forms without using any vibrators. In order to place concrete into reinforced members, concrete should have segregation resistance and high flowability. In this study, the binder of concrete, such as Ordinary Portland Cement, fly ash, and blast furnace slag, cement were mixed with the addition of superplasticizers and tested their flowability and segregation resistance using slump flow tester and L type flow tester. As a results, High Performance Concrete can be made using Portland blast furnace slag cement along with superplasticizers but the slump-loss of concrete is so large that measure should be made.

• Proceedings of the Korea Concrete Institute Conference
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• 2010.05a
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• pp.427-428
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• 2010

Recently, building structure damage and number of lives lost by bomb terror is increasing. Therefore, in this study, it is aimed to present basic data for blast resistance performance of concrete member with intermediate space by fiber reinforced mortar panel by explosion test.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2021.11a
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• pp.226-227
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• 2021

Various attempts are being made to reduce carbon emissions through recycling of industrial by-products in the construction materials industry to reduce carbon emissions, and cement substitutes such as blast furnace slag and fly ash are widely used. Although it is suggested that the use of industrial by-product aggregate is possible in 'Aggregate', the use case of industrial by-product aggregate is very rare in the actual field. In this study, as an industrial by-product, fine slag aggregate is used as fine aggregate among aggregates that can be used as aggregate for concrete, and coarse aggregate is used as a substitute for natural aggregate. WWe tried to suggest various ways to expand the use of industrial by-product aggregates.

• Proceedings of the Korea Concrete Institute Conference
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• 1999.10a
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• pp.763-766
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• 1999

The objective of this study was to investigate the influence of low temperature curing on resistance of freezing and thawing of self-compacting concrete placed in cold weather regions. The experimental study results indicated that the self-compacting concrete incorporating ground granulated blast-furnace slag showed good resistance to freezing and thawing, and the self-compacting concrete cellulose viscous agent had relatively poor resistance to freezing and thawing.