• Asian Pacific Journal of Cancer Prevention
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• 제15권8호
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• pp.3471-3476
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• 2014

Background: Aberrant expression of the microRNA-29 family is associated with tumorigenesis and cancer progression. As transport carriers, tumor-derived exosomes are released into the extracellular space and regulate multiple functions of target cells. Thus, we assessed the possibility that exosomes could transport microRNA-29c as a carrier and correlations between microRNA-29c and apoptosis of bladder cancer cells. Materials and Methods: A total of 28 cancer and adjacent tissues were examined by immunohistochemistry to detect BCL-2 and MCL-1 expression. Disease was Ta-T1 in 12 patients, T2-T4 in 16, grade 1 in 8, 2 in 8 and 3 in 12. The expression of microRNA-29c in cancer tissues was detected by quantitative reverse transcriptase PCR (QRT-PCR). An adenovirus containing microRNA-29c was used to infect the BIU-87 human bladder cancer cell line. MicroRNA-29c in exosomes was measured by QRT-PCR. After BIU-87 cells were induced by exosomes-derived microRNA-29c, QRT-PCR was used to detect the level of microRNA-29c. Apoptosis was examined by flow cytometry and BCL-2 and MCL-1 mRNA expressions were assessed by reverse transcription-polymerase chain reaction. Western blotting was used to determine the protein expression of BCL-2 and MCL-1. Results: The expressions of BCL-2 and MCL-1 protein were remarkably increased in bladder carcinoma (p<0.05), but was found mainly in the basal and suprabasal layers in adjacent tissues. The expression of microRNA-29c in cancer tissues was negatively correlated with the BCL-2 and MCL-1. The expression level of microRNA-29c in exosomes and BIU-87 cells from the experiment group was higher than that in control groups (p<0.05). Exosome-derived microRNA-29c induced apoptosis (p<0.01). Although only BCL-2 was reduced at the mRNA level, both BCL-2 and MCL-1 were reduced at the protein level. Conclusions: Human bladder cancer cells infected by microRNA-29c adenovirus can transport microRNA-29c via exosomes. Moreover, exosome-derived microRNA29c induces apoptosis in bladder cancer cells by down-regulating BCL-2 and MCL-1.

• 보험의학회지
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• 제32권1호
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• pp.21-27
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• 2013

In case of neoplasm claims, it is important to make a decision of differentiating malignant and benign. In Korean insurance market, there are many insurance products that cover cancer. In the insurance claims adjustment, differentiation between malignant and benign is according to histologic findings. However there are many neoplasms of bad clinical course in spite of benign histopathologic classification. In this article; astrocytoma, thymoma, gastrointestinal stromal tumor, colonic intramucosal carcinoma, gastric high grade adenoma/dysplasia, carcinoid tumor, MALT lymphoma, revision of Korean Classification of Disease-6th edition, and bladder tumors are reviewed in terms of differentiation between malignant and benign in the insurance claims. It may be helpful for claims staff to review important neoplasms in terms of differentiation between malignant and benign.

• Journal of Chest Surgery
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• 제25권10호
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• pp.1030-1034
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• 1992

There are follow-up data according to thirteen patients recieved the surgical resection for metastatic lung cancer arising from different primary tumor. The patients were received the surgical resection at Korean Cancer Center Hospital from July 1987 to Setember 1991 and followed-up to August 1992. There were 9 men and 4 women, ranging in age from 16 to 70 years[mean age, 42.8 years]. The primary tumors were 2 synovial sarcoma, 2 sarcoma, 2 osteosarcoma, 3 laryngeal ca, 1 melanoma, 1 ovarian ca and 1 bladder ca. The operative procedures were 5 wedge resections, 1 segmental resection, 5 lobectomies, 1 bil-obectomy and 1 pneumonectomy. There was no operative and hospital death. There were 3 deaths[each survival period: 2, 9 and 20 months, average 10.3 months]and 5 tumor recurrence during follow-up. At now, the average survival period of aliving patients is 29.1 months.

• 대한골관절종양학회지
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• 제15권1호
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• pp.75-80
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• 2009

골수외 형질세포종은 아주 드물게 발생하는 종양으로, 전체 형질 세포 종양의 3~5 %를 차지하며 상부 호흡기관에서 주로 발생한다. 그 외 드물게 위장관, 방광, 중추 신경계, 유방, 갑상선, 고환, 타액선, 임파선, 피부 등에서 발생한 증례보고가 있다. 저자들은 49세 남자의 대둔근에서 발생한 골수외 형질세포종 1예를 경험하였기에 이를 문헌 고찰과 함께 보고하는 바이다.

• 대한세포병리학회지
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• 제6권2호
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• pp.148-155
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• 1995

Urinary cytology has become an essential element in the diagnosis and management of transitional ceil carcinoma(TCC) of the urinary tract. It has the advantage of being noninvasive, inexpensive, and easily accessible. Besides that it can even detect malignancy when unsuspected at cystoscopy. We report a retrospective review of urine cytology un the diagnosis of 83 TCC cases that underwent 295 cytologic evaluation. All patients had biopsy-proven TCC of the bladder, ureter and renal pelvis, The overall incidence of the positive cytology cases was 66.2%. To define the cytologic features of tumor cells, we tried to use three cytologic gradings such as "grade 1", "grade 2", and "grade 3" according to the cytologic degree of anaplastic neoplastic cells. These cytologic gades of TCC were relatively well correlated with the histologic grade and tumor invasiveness. This result suggests that the recognition of characteristic cellular features of TCC can suspect the histologic grade and tumor stage. The false negative TCC cases were 78.9%. They showed severe inflammatory or bloody background and a few neoplastic cells. Therefore, a cautious approach for accurate interpretation, personal experience, and proper fixation and processing could expand the role of urinary cytology.

• 한국응급구조학회지
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• 제24권3호
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• pp.147-154
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• 2020

Purpose: The purpose of the study was to investigate traumatic urethral injury in a 63-year-old patient with hematuria. Methods: A hematuria patient was transferred by paramedics. At the time of the visit, the patient's blood pressure (151/91mmHg), pulse rate (86/min), body temperature (37.1℃), and other vital signs were stable. Their KTAS (Korean Triage and Acuity Scale) was Level 4. The patient had no damage to the injured area, but a large contrast defect was observed between the prostate urethra and the bladder in urethral angiography performed due to persistent hematuria and pain in the injured area. Results: Following radiological evaluation of a suspected liposarcoma or neuroma mass of the prostate urethra, the mass was removed through urethral tumor resection. The result of histologic evaluation provided a diagnosis of highly differentiated invasive urethral cell carcinoma that had invaded the muscle layer. The patient was given additional treatment for urethral cancer but was rejected and is currently being followed. Conclusion: The prognosis for urinary tract cancer has distinct differences for patients with lymph node metastasis and tumor characteristics. The presence or absence of urethral cancer should be confirmed through angiography, CT, MRI, and cystoscopy.

• Asian Pacific Journal of Cancer Prevention
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• 제15권4호
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• pp.1517-1520
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• 2014

Aim: To investigate effects of sulforaphane on the BIU87 cell line and underlying mechanisms involving IGFBP-3. Methods: Both BIU87 and IGFBP-3-silenced BIU87 cells were treated with sulforaphane. Cell proliferation was detected by MTT assay. Cell cycle and apoptosis were determined via flow cytometry. Quantitative polymerase chain reaction and Western blotting were applied to analyze the expression of IGFBP-3 and NF-${\kappa}B$ at both mRNA and protein levels. Results: Sulforaphane (80 ${\mu}M$) treatment could inhibit cell proliferation, inducing apoptosis and cell cycle arrest at G2/M phase. All these effects could be antagonized by IGFBP-3 silencing. Furthermore, sulforaphane (80 ${\mu}M$) could down-regulate NF-${\kappa}B$ expression while elevating that of IGFBP-3. Conclusions: Sulforaphane could suppress the proliferation of BIU87 cells via enhancing IGFBP-3 expression, which negatively regulating the NF-${\kappa}B$ signaling pathway.

• 한국수의병리학회지
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• 제1권2호
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• pp.107-118
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• 1997

The anticarcinogenic effects of allyl methyl trisulfide(AMT) and methyl propyl disulfide(MPD) were studied in a 28 weeks rat multi-organ carcinogenesis model. Tumor incidence rate was decreased by AMT or MPD treatment comparing with the positive control. AMT treatment significantly decreased the incidence of neoplastic and preneoplastic lesions in the kidney thyroid gland urinary bladder alimentary tract lung and Zymbal's gland. MPD also inhibited incidence of noplastic and preneoplastic lesions in the liver kidney urinary bladder alimentary tract lung and Zymbal's gland but increased that in the thyroid gland. GST-p positive foci in the liver were slightly decreased by AMT or MPD. There was no significant histopathological lesions in AMT or MPD treated group without pretreatment of carcinogens.

• Clinical and Experimental Reproductive Medicine
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• 제11권1호
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• pp.9-16
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• 1984

This study was conducted to determine whether patients could engage in sexual activity following total cystectomy with ileal conduit. Sixteen male bladder cancer patients treated by total cystectomy with or without preoperative irradiation. Patients ranged in age from 48 to 70 years (mean 59.6 years). The interval from therapy ranged from 7 to 82 months (mean 33.8 months).Prior to therapy 11 /16 patients (68.8 %) reported they were engaging some form of sexual activity at least once per month. All were able to achieve an erection and were orgasmic. Following total cystectomy, 6 patients (37.5%) continued to engage in sexual activity at least once per month and were experiencing orgasm. 3 patients (18.8%) reported they were able to achieve an erection. The spontaneous resumptiom of sexual activity by almost one third of the patients in this study refutes the assumption that sexual activity would be impossible following total cystectomy.

• Journal of Radiation Protection and Research
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• 제32권3호
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• pp.105-110
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• 2007

최근 CT, MRI, PET등 영상진단기술 및 방사선치료계획 소프트웨어 등이 획기적으로 발전하였음에도 불구하고 자궁경부암의 강내조사는 아직까지 A점 등 ICRU 38에 근거한 치료계획을 보편적으로 사용하고 있다. CT를 이용한 3차원 강내조사 계획은 종양 및 정상조직의 선량-용적 히스토그람(DVH)에 대한 정보를 제공한다. 본 연구에서는 CT를 이용하여 표적용적(CTV)에 목표선량을 조사하는 치료계획(CTV 치료계획)과 ICRU38에 근거한 치료계획(ICRU 치료계획)을 시행하고, 각각에 대한 DVH를 분석하여 두 치료계획간의 종양선량, 직장선량, 방광선량 등을 비교하였다. Ir-192 고선량율강내치료(HDR)를 받은 11명의 환자를 대상으로 하였다. 강내조사 치료계획은 외부방사선치료를 일일선량 180cGy씩 4문조사(Box technique)로 약 40Gy 시행한 후 수립되었으며 모든 환자에서 CT 모의치료기를 이용한 CT가 시행되었고 치료계획은 PLATO(Nucletron) v.14.2를 이용하였다. CT 영상에 CTV, 직장, 방광 등을 도시한 후 CTV에 100%의 선량을 조사하는 치료계획 및 ICRU 38에 근거하여 A점에 100%를 조사하는 치료계획을 수립하였다. 11명 환자의 CTV 용적(평균${\pm}$표준편차)은 $21.8{\pm}26.6cm^3$, 직장 용적은 $60.9{\pm}25.0cm^3$, 방광용적은 $111.6{\pm}40.1cm^3$이었으며, 100%의 선량이 포함하는 용적은 ICRU 치료계획에서는 $126.7{\pm}18.9cm^3$, CTV 치료계획에서는 $98.2{\pm}74.5cm^3$이었다. (p=0.0001). ICRU 치료계획 시 잔류종양의 크기가 4cm 이상인 1례에서는 CTV 용적 $22.0cm^3$가 100% 등선량곡선에 포함되지 않았으며 잔류종양의 크기가 4cm 미만인 나머지 10례에서는 종양용적 이외의 정상조직 $62.2{\pm}4.8cm^3$이 불필요하게 100% 이상의 선량이 조사되었다. ICRU 38의 권고에 따른 방광선량은 ICRU 치료계획 및 CTV 치료계획에서 각각 $90.1{\pm}21.3%,\;68.7{\pm}26.6%$이었고(p=0.001), 직장선량은 $86.4{\pm}18.3%,\;76.9{\pm}15.6%$이었다(p=0.08). 방광 및 직장선량의 최대 점선량 또한 ICRU 치료계획과 CTV계획에서 각각 $137.2{\pm}50.1%$ vs $107.6{\pm}47.9%$, (p=0.008), $101.1{\pm}41.8%$ vs $86.9{\pm}30.8%$ (p=0.045) 로서 CTV 치료계획에서 정상조직에 조사되는 선량이 더 적게 나타났다. 그러나 잔류종양이 4cm 이상인 환자에서는 CTV 치료계획에서 정상조직 선량이 권고 선량보다 현저히 높게 나타났다. 방광 및 직장의 용적선량에서는 투여선량의 80% 이상을 받는 직장용적선량(V80rec)은 ICRU 치료계획 및 CTV 치료계획에서 각각 $1.8{\pm}2.4cm^3,\;0.7{\pm}1.0cm^3$(p=0.02), 방광용적선량(V80bla)은 $12.2{\pm}8.9cm^3,\;3.5{\pm}4.1cm^3$로서 역시 CTV 치료계획에서 적게 조사되었다(p=0.005). 기존의 ICRU 치료계획은 잔류종양의 크기가 작은 경우 불필요하게 정상조직에 많은 선량이 투여되기 때문에 CT를 이용한 CTV 치료계획을 적용하여 정상조직에 대한 피폭을 현저히 낮추고 잔류종양에 목표한 선량을 조사할 수 있다. 다만 잔류종양의 크기가 큰 경우에는 정상조직에 대한 조사선량을 줄이기 위한 효과적 치료계획에 대한 연구가 필요할 것으로 판단된다.