• Title/Summary/Keyword: bladder function

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L-Arginine Ameliorates Kidney Function and Urinary Bladder Sensitivity in Experimentally-induced Renal Dysfunction in Rats

  • Mansour, Mahmoud A.;Al-Shabanah, Othman A.;El-Khashef, Hassan A.
    • BMB Reports
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    • v.36 no.4
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    • pp.373-378
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    • 2003
  • Effects of L-arginine and NG-nitro-L-arginine methyl ester (L-NAME) on the renal dysfunction that is induced by cisplatin (CDDP) were investigated. A single dose of CDDP (7.5 mg/kg i.p.) induced renotoxicity, which was manifested by increasing the sensitivity of isolated urinary bladder rings to acetylcholine (ACh), together with a significant elevation of serum urea and creatinine, and a severe decrease in serum albumin. Moreover, renal dysfunction was further confirmed by a significant decrease of enzyme activities, such as glutathione peroxidase, GSH-Px (E.C 1.11.1.9), catalase (E.C 1.11.1.6), as well as a significant increase in lipid peroxides that were measured as malondialdhyde (MDA) in kidney tissue homogenates. The administration of L-arginine (70 mg/kg/d p.o in drinking water 5 d before and 5 d after the CDDP injection) significantly ameliorated the renotoxic effects of CDDP, as judged by restoring the normal responses of isolated bladder rings to Ach, and also by an improvement in a range of renal function indices, which included serum urea and creatinine concentrations and kidney weight. In addition, L-arginine prevents the rise of MDA, as well as a reduction of GSH-Px and catalase activities in kidney tissues homogenates. On the other hand, the administration of L-NAME (4 mg/kg/d p.o) resulted in no protection against renal dysfunction that was induced by CDDP treatment. The findings of this study suggest that L-arginine can attenuate kidney injury that is produced by CDDP treatment. In addition, L-arginine may be a beneficial remedy for CDDP-induced renal toxicity, and could be used to improve the therapeutic index of CDDP.

Benzidine Induces Epithelial-Mesenchymal Transition of Human Bladder Cancer Cells through Activation of ERK5 Pathway

  • Sun, Xin;Zhang, Tao;Deng, Qifei;Zhou, Qirui;Sun, Xianchao;Li, Enlai;Yu, Dexin;Zhong, Caiyun
    • Molecules and Cells
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    • v.41 no.3
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    • pp.188-197
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    • 2018
  • Benzidine, a known carcinogen, is closely associated with the development of bladder cancer (BC). Epithelial-mesenchymal transition (EMT) is a critical pathophysiological process in BC progression. The underlying molecular mechanisms of mitogen-activated protein kinase (MAPK) pathway, especially extracellular regulated protein kinases 5 (ERK5), in regulating benzidine-induced EMT remains unclarified. Hence, two human bladder cell lines, T24 and EJ, were utilized in our study. Briefly, cell migration was assessed by wound healing assay, and cell invasion was determined by Transwell assay. Quantitative PCR and western blot were utilized to determine both gene expressions as well as protein levels of EMT and MAPK, respectively. Small interfering RNA (siRNA) was transfected to further determine ERK5 function. As a result, the migration and invasion abilities were enhanced, epithelial marker expression was decreased while mesenchymal marker expression was increased in human BC cell lines. Meanwhile, benzidine administration led to activation of ERK5 and activator protein 1 (AP-1) proteins, without effective stimulation of the Jun N-terminal kinase (JNK) or p38 pathways. Moreover, Benzidine-induced EMT and ERK5 activation were completely suppressed by XMD8-92 and siRNAs specific to ERK5. Of note, ERK1/2 was activated in benzidine-treated T24 cells, while benzidine-induced EMT could not be reversed by U0126, an ERK1/2 inhibitor, as indicated by further study. Collectively, our findings revealed that ERK5-mediated EMT was critically involved in benzidine-correlated BC progression, indicating the therapeutic significance of ERK5 in benzidine-related BC.

Excretory bladder: the source of cysteine proteases in Paragonimus westermani metacercariae

  • Yang, Hyun-Jong;Chung, Young-Bae;Kang, Shin-Yong;Kong, Yoon;Cho, Seung-Yull
    • Parasites, Hosts and Diseases
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    • v.40 no.2
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    • pp.89-92
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    • 2002
  • The cysteine proteases of Paragonimus westermani metacercariae are involved in metacercarial excystment, host immune modulation, and possibly in tissue penetration. In order to clarify the origin of the enzymes, 28 and 27 kDa cysteine proteases in metacercarial excretory-secretory products were purified through the FPLC system using Mono Q column chromatography. The polyclonal antibodies to the enzymes were produced in BALB/c mice. Immunolocalization studies revealed that both cysteine proteases were distributed at the linings of excretory bladder and excretory concretions of the metacercariae. It was suggested that the excretory epithelium of P. westermani undertake the secretory function of metacercarial cysteine proteases, in addition to its role as a route for eliminating waste products.

Histological Examination of Engineered Mesenchymal Stem Cells Improve Bladder Function in Rat (랫드에서 방광기능 향상의 엔지니어링 중간엽 줄기세포의 조직학적 소견)

  • Cho, Eun Kyung;Jeon, Seung Hwan
    • Korean Journal of Clinical Laboratory Science
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    • v.52 no.2
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    • pp.112-118
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    • 2020
  • This study was undertaken to examine the effects and to investigate the relevant mechanisms of overexpressing stromal cell-derived factor-1 (SDF-1) produced by engineered mesenchymal stem cells, in a neurogenic bladder (NB) rat model. Sprague-Dawley (SD) rats (N=48) were randomly divided into 4 groups comprising 12 rats each: control group, Injury group, Injury+imMSC group, and Injury+SDF-1 eMSC group. Rats in the Injury+imMSC group were treated with imMSCs, whereas the Injury+SDF-1 eMSC group were administered SDF-1 eMSCs. After 4-weeks therapy, the bladder and pelvic nerve (PN) tissues were examined by subjecting to Masson's trichrome staining and immunofluorescence. Administration of SDF-1 eMSC resulted in improved smooth muscle content in the bladder tissue, significantly increased β-III tubulin expression of the PN, and enhanced SDF-1 expression (P<0.05). The bladder wall repair can be attributed to the overexpression of SDF-1 by SDF-1 eMSCs. Significantly increased SDF-1 expression was obtained in the Injury+SDF-1 eMSC group (P<0.05). The crushed PN also showed significant recovery in the Injury+SDF-1 eMSC group (P<0.05). In conclusion, our results indicate that SDF-1 eMSCs express more SDF-1 in vivo, thereby facilitating the repair of injured nerve and recovery of NB in rats.

Intra-Spinal Bone Marrow Mononuclear Cells Transplantation Inhibits the Expression of Nuclear Factor-${\kappa}B$ in Acute Transection Spinal Cord Injury in Rats

  • Shrestha, Rajiv Prasad;Qiao, Jian Min;Shen, Fu Guo;Bista, Krishna Bahadur;Zhao, Zhong Nan;Yang, Jianhua
    • Journal of Korean Neurosurgical Society
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    • v.56 no.5
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    • pp.375-382
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    • 2014
  • Objective : To assess the effect of bone marrow mononuclear cells (BMMNCs) transplantation in the expression of nuclear factor-${\kappa}B$ (NF-${\kappa}B$) in spinal cord injury (SCI) in rats. Methods : BMMNCs were isolated from tibia and femur by a density gradient centrifugation. After establishment of acute transection SCI, rats were divided into experiment (BMMNCs), experiment control (0.1 M PBS infused) and sham surgery groups (laminectomy without any SCI). Locomotor function was assessed weekly for 5 weeks post-injury using BBB locomotor score and urinary bladder function daily for 4 weeks post-injury. Activity of NF-${\kappa}B$ in spinal cord was assessed by immunohistochemistry and reverse transcriptase polymerase chain reaction. Results : At each time point post-injury, sham surgery group had significantly higher Basso, Beattie, Bresnahan locomotor and urinary bladder function scores than experiment and experiment control group (p<0.05). At subsequent time interval there were gradual improvement in both experiment and experiment control group, but experiment group had higher score in comparison to experiment control group (p<0.05). Comparisons were also made for expression of activated NF-${\kappa}B$ positive cells and level of NF-${\kappa}B$ messenger RNA in spinal cord at various time points between the groups. Activated NF-${\kappa}B$ immunoreactivity and level of NF-${\kappa}B$ mRNA expression were significantly higher in control group in comparison to experiment and sham surgery group (p<0.05). Conclusion : BMMNCs transplantation attenuates the expression of NF-${\kappa}B$ in injured spinal cord tissue and thus helps in recovery of neurological function in rat models with SCI.

Study on the effect of Yukmijihwangwon(YMJHW) and Taeyoungjeon(TYJ) extract on the Meridian of Human body and Active oxygen (육미지황원(六味地黃元) 및 대영전(大營煎)이 인체경락기능(人體經絡機能)과 활성산소(活性酸素)에 미치는 영향(影響)에 관(關)한 실험적(實驗的) 연구(硏究))

  • Cho, Ki-Yong;Yoo, Dong-Yeol
    • The Journal of Korea CHUNA Manual Medicine
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    • v.1 no.1
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    • pp.91-102
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    • 2000
  • To prove the effect of Yukmijihwngwon(YMJHW) and Taeyoungjeon(TYJ) extract on the Meridian of Human body and Active oxygen experimentally, ORS & Free Radical of extract and precipitate of YMJHW and TYJ was measured. The results were summarized as follows; 1. TYJ extract, as a result of measuring QRIS, had a considerable effect on the function of pituitary gland, the 4th cervical vertebra and the 1 st lumbar vertebra. 2. TYJ precipitate, as a result of measuring QRIS, had a considerable effect on the function of liver, uterus, and vertebra. 3. YMJHW extract, as a result of measuring QRIS, had a considerable effect on the function of lung, adrenalin gland, bladder, prostate gland, ovaria, the 2nd cervical vertebra, the 3rd cervical vertebra, the 5th cervical vertebra, the 6th cervical vertebra, the 2nd lumbar vertebra, the 4th lumbar vertebra and the 5th lumbar vertebra. 4. YMJHW precipitate, as a result of measuring QRIS, had a considerable effect on the function of spleen, lung, kidney, adrenal gland, bladder, ovaria, the 2nd cervical vertebra, the 6th cervical vertebra, the 7th cervical vertebra, the 2nd lumbar vertebra, the 5th lumbar vertebra, sacrum, and knee joint. 5. As a result of measuring Free Radical, the level of TYJ is higher than YMJHW. It is thiut that's because of Duchung and roasted Gamcho. 6. As an order of QRIS levels, YMJHW precipitate is the highest, YMJHW extract is next, TYJ extract and TYJ precipitate. These results suggested that TYJ and YMJHW be usefully applied for suppressing of abnormal Meridian and noxious oxygen, preventing and curing all the disease.

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Effectiveness of a Custom-made Multi Purpose Brachytherapy Phantom (자체 제작한 근접방사선치료용 다목적 팬텀의 유용성 평가)

  • Jang, In-Gi;Lee, Jin-Joung;Kim, Wan-Sun
    • The Journal of Korean Society for Radiation Therapy
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    • v.18 no.2
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    • pp.119-125
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    • 2006
  • Purpose: To measure the dose for dose optimization at the reference point (A, B) and the critical organ with multi Purpose brachytherapy phantom (MPBP). For this wort the MPBP was custom made, and designed to reconstruct the treatment applicator using multi function applicator (MFA) in the same way as the treatment of patient. Materials and Methods: Dose measurements were made at the reference points (A, B) and the bladder with thermoluminescence dosimeter (TLD) for four patients with tandem and ovoid of uterine cervix cancer using the phantom. In Phantom, Total 20 times of the measurements were made with 5 times a patient. Results: The results of TLD measurements in MPBP phantom showed the relative error ranging from -3.2% to 3.8% at A point, and -1.4% to 4% at B point and 1.3% to 7.15% at the bladder of reference point. Conclusion: The reproducibility of dose measurement under the same condition as the treatment could be achieved using the custom-made MFA in phantom and the dose at the reference point (A, B) and bladder could be analyzed accurately. The measured dose acquired in MPBP can apply for the dose optimization.

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Physical Therapy for Multiple Sclerosis (다발성 경화증의 물리치료)

  • Kim, Young-Min
    • Journal of Korean Physical Therapy Science
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    • v.2 no.2
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    • pp.533-544
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    • 1995
  • Multiple sclerosis is a chronic, pregressive, demyelinating, disease of the central nervous system. It is named for the formation of disseminated scarlike lesions primarily in the central white mattrer of the brain and spinal cord. These plaques are commonly found in the regions of the optic tracts, third and fourth ventricles, basal ganglia, midbrain, pons, and spinal cord. Multiple sclerosis is an unpredictable disease, typically presenting with an exacerbating-remitting course, although other clinical courses have been recognized. Common clinical findings include disturbances in sensation, muscle strength, tone, fatigue, coordination, vision, communication, bladder and bowel function, and cognitive and behavioral function. Physical therapy of the patient with multiple sclerosis is centered around decreasing symptoms, improving function, prevention secondary complications, and promoting successful psychological adjustment. It requires the comprehensive efforts of a health care team to provide coordinated and continuing care.

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Function Disease Symptom And Organ Coordination of Tri-Energizer Based on the Materiality of Tri-Energizer (삼초유형(三焦有形)으로 살펴본 삼초(三焦)의 기능(機能), 병증(病證) 및 장부배합(臟腑配合))

  • Yoon, Chang-Yeol
    • Journal of Korean Medical classics
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    • v.26 no.2
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    • pp.1-7
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    • 2013
  • Objective & Method : By investigating physiological function of tri-energizer, symptoms, and organ coordination, we obtained following conclusion. Tri-energizer is the membrane structure that surrounds the five visceral organs and six hollow organs, and filled with fluid. Tri-energizer acts as the passage for the flow of qi energy. Result & Conclusion : Therefore, dysfunction of the tri-energizer is caused by abnormal evaporation and metabolism. Upper-energizer regulates cardiopulmonary function, middle-energizer regulates spleen and stomach functions, and lower-energizer regulates liver, kidneys, small and large intestines, and bladder functions. Such a functional specialization is possible by receiving the source of qi through the wall wrapping around the internal organs. Tri-energizer represents the exterior and interior relationship by acting as the membrane structure supporting the five visceral organs and six hollow organs and at the same time, as the pericardium surrounding the heart.

Study on Muscular System about Gall Bladder Channel of Foot Soyang Muscle (족소양담경근(足少陽膽經筋)에 대한 근육학적 고찰)

  • Ryu, Hyung-Sun;Kang, Jung-Soo
    • Journal of Acupuncture Research
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    • v.22 no.5
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    • pp.29-36
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    • 2005
  • Objectives : This study is performed to understand the interrelation between 'Foot soyang muscle of the Gall bladder channel' and 'muscular system' on the basis of the link between meridian muscle theory and myofascial pain syndrome. Methods : We have researched some of oriental medical books about meridian muscle theory and western medical books about anatomical muscular system. Results & Conclusion : 1. Myofascial pain syndrome is the medical treatment which finds the start point of the pain in fascia and then treats it on the basis of object and concrete anatomical theory, so its application is needed for objectification of the oriental medicine. 2. There is a wide difference between myofascial pain syndrome and meridian muscle theory in that the former explains each muscle individually, while the latter classifies muscles systematically in the view of organism. 3. Foot soyang muscle contains Dorsal interosseous m, Extensor digitorum longus m, Musculus peroneus brevis, longus and, tertius, lliotibial tract, Vastus lateralis m, Gluteus m, Aximus m, Piriformis m, Tensor fasciae latae m, Gluteus minimus m, Obliquus internus & externus abdominis m, External & Internal intercostal m, Serratus anterior m, Pectoralis major m, Sternocleidomastoid m, Auricularis posterior m, Temporalis m, Masseter m, Orbicularis oculi m etc. on the basis of function and the nature of a disease reflected in muscle. 4. Foot soyang muscle keeps the balance of left md right of the body on the outside, while the Gall bladder keeps the balance of the JangBuKiHyeul(臟腑氣血) on the inside.

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