• 대한세포병리학회지
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• 제18권2호
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• pp.153-156
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• 2007

Primary small cell carcinoma of the urinary bladder is an extremely rare but important entity. We experienced a case of small cell carcinoma of the urinary bladder diagnosed by urine cytology. A 59-year-old man presented with gross hematuria and dysuria, and a calcified mass was detected at the left ureterovesical junction by cystoscopy. Abdominal ultrasonography revealed focal wall thickening at the left lateral side of the urinary bladder, and urine cytology findings were of an inflammatory background and atypical small round cells with minute hyperchromatic or pyknotic nuclei, scant cytoplasm, and rare nucleoli. In addition, atypical cells were scattered in an isolated single cell pattern or in small loose clusters with prominent nuclear molding. Subsequent histological and immunohistochemical examinations confirmed a diagnosis of small cell carcinoma.

• Journal of Animal Science and Technology
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• 제54권6호
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• pp.419-425
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• 2012

The coordinated action of the detrusor muscle cells in the urinary bladder is governed by cell-cell communication through gap junction, consisted of connexin (Cx) molecules. Even though a number of researches have been mostly focused on expressional changes of a few Cx isoforms in clinically dysfunctional condition of the bladder, less attention has been paid for investigation of Cx isoforms present in the bladder. Using real-time PCR analysis, the present study examined Cx isoforms expressing in the male rat bladder during postnatal period. Also, expressional patterns of Cx isoforms were evaluated in the bladder at different postnatal ages. Of a total of 13 Cx isoforms tested in the present study, we were able to detect mRNAs of 6 Cx isoforms in the rat urinary bladder, including Cxs 31, 31.1, 32, 37, 40, and 45. The transcript levels of Cxs 31, 31.1, 37, 40, and 45 were gradually increased from 1 week of age until 25 days of age, followed by transient decreases at 45 days of age. However, abundance of Cx32 transcript was drastically increased at 15 days of age, followed by a sharp drop at 45 days of age. These results indicate that differential expression of Cx isoforms in the bladder during postnatal development would be necessary for maintaining proper function of the bladder. A question remains to be answered if significant decreases of transcript levels of some Cx isoforms at the elderly are associated with age-dependent dysfunction of the bladder.

• Asian Pacific Journal of Cancer Prevention
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• 제13권8호
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• pp.3549-3553
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• 2012

Primary small cell carcinoma of urinary bladder is a rare but aggressive disease with poor prognosis and a high mortality rate. It accounts for less than 1 % of all the primary cancers seen in the urinary bladder. Diagnosis and management of this entity poses a challenge to the clinician due to the lack of a standardized protocol for its treatment. Herein we discuss primary small cell carcinoma of the urinary bladder in its entirety.

• 사상체질의학회지
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• 제20권2호
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• pp.85-97
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• 2008

1. Objectives The purpose of this study is to investigate the anti-aging and anti-oxidant effects of Hyeongbangjiwhang-tang decoction(HGD) in SD rats. 2. Methods This experiment was used the tissue of kidney, bladder and spleen cells of 6, 52 and 68 weeks old SD rats. Each age group was again divided into three groups. One group, as normal group, was not-treated cells, another group, as control group, was saline-treated cells, and the last group, as experimental group, was HG-treated cells. After culture for 48 hours, each groups measured the level of SOD, GSH, MDA and NO in the tissue of kidney, bladder and spleen cells. 3. Results and Conclusions The activity of SOD were significantly increased in kidney cell of 68 w-HGD group, bladder cell of 52 w-HGD group and in spleen cell of 52,68 w-HGD group compared with those of the control groups. The level of GSH were significantly increased in bladder cell of 52w-HGD group and in spleen cell of 68w-HGD group compared with those of the control groups. The level of MDA were significantly decreased in bladder cell of 68 w-HGD group and in spleen cell of 68w-HGD group compared with those of the control groups. The level of NO were significantly decreased in kidney cell of 68 w-HGD group, bladder cell of 52, 68w-HGD groups, and in spleen cell of 68w-HGD group compared with those of the control groups. These results suggest that HGD(Hyeongbangjiwhang-tang) has anti-oxidant effects in aged rats.

• 대한세포병리학회지
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• 제5권2호
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• pp.167-171
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• 1994

Small cell carcinoma of the urinary bladder is a rare tumor which occurs in about 0.48 % of all bladder tumors. We report cytologic features of small cell carcinoma of the urinary bladder in a 66-year-old man who had painless total gross hematuria, which was confirmed by partial cystectomy. In urine cytology, abundant tumor cells appeared in scattered and clustered forms in a bloody background. The tumor cells were small and uniform in size with a high nuclear/cytoplasmic ratio. The nuclei of the tumor cells were hyperchromatic, characteristically molded and showed inconspicuous nucleoli. The cytoplasms were scanty and pale blue.

• Asian Pacific Journal of Cancer Prevention
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• 제16권9호
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• pp.3973-3976
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• 2015

Objectives: To explore the expression of aquaporin 1 ($AQP_1$) in bladder uroepithelium cell carcinoma (BUCC) and its relevance to recurrence. Materials and Methods: Tissue samples from 45 BUCC patients who underwent total cystectomy or transurethral resection of bladder tumor (TURBT) and from 40 patients with non-bladder cancers who underwent special detection or treatments were collected. The level of expression of $AQP_1$ in BUCC tissues and normal bladder tissues was assessed by immunohistochemistry so as to analyze the relevance to pathological patterns and time of recurrence in BUCC patients. Results: The expression levels of $AQP_1$ normal bladder tissues and BUCC tissues were $2.175{\pm}0.693$ and $3.689{\pm}0.701$, respectively, and the difference was significant (t=9.99, P<0.0001). Marked increase was noted with BUCC histological grade and pathological stage (P<0.01). Moreover, the expression of $AQP_1$ was evidently higher in cancerous tissues with lymph node metastasis than in those without (P<0.01). With short-term recurrence, the positive cell expression rate of $AQP_1$ was higher in primary tissues, which increased obviously after recurrence. Additionally, the recurrent time of BUCC was negatively associated with the positive cell expression rate of $AQP_1$ and the difference between the expression of $AQP_1$ before and after recurrence (r=-0.843, F=39.302, P=0.000; r=-0.829, F=35.191, P=0.000). Conclusions: $AQP_1$, which reflects the grade, stage, lymph node metastasis and recurrence of BUCC, has potential guiding significance in the diagnosis and treatment of bladder cancarcinoma.

• 대한세포병리학회지
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• 제10권1호
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• pp.67-71
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• 1999

We report a case of 53-year-old man with plasmacytold transitional cell carcinoma of the urinary bladder, which may be confused with plasmacytoma. The patient initially presented with gross hematuria and dysuria for two months. Cystoscopy and radiologic studios revealed multiple intraluminal protruding masses on the urinary bladder invading perivesical fat tissue. After urinary cytologic examination and cystoscopic biopsy, radical cystectomy and pelvic lymph node dissections were done. Urine cytology showed single cells and poorly cohesive cells with round eccentric nuclei, bi-or multi-nucleation, indistinct nucleoli, coarse chromatin, and abundant basophilic cytoplasm within relatively clear background. The cytologic findings of tumor cells were similar to the plasma cells seen in plasmacytoma. The tumor of the bladder was composed on discohesive, individual cancer cells with diffuse pattern that simulated lymphoma or plasmacytoma. Immunohistochemical and electron microscopic studies clearly established the epithelial nature of the neoplasm. Recognition of this plasmacytoid type of transitional cell carcinoma of the urinary bladder can avoid the misdiagnosis.

• 대한수의학회지
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• 제63권4호
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• pp.39.1-39.5
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• 2023

A 14-year-old, spayed female, poodle was presented with dysuria and hematuria. A mass that appeared hypoechoic on ultrasound and hypoattenuating on computed tomography (CT) extended from the bladder neck to the urethra. Magnetic resonance imaging (MRI) showed the mass invading the muscular layer of the bladder, urethra, and prostate with distinct margins. Transitional cell carcinoma (TCC) was confirmed with the CADET-BRAF test. This study describes the CT and MRI features of suspected TCC lesions involving the bladder, prostate, and urethra. MRI showed superior soft tissue contrast resolution, enabling evaluation of invasion of the muscular layer of the bladder and urethra.

• The Korean Journal of Physiology and Pharmacology
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• 제25권2호
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• pp.119-129
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• 2021

Bladder cancer is one of the most common types of cancer. Most gene mutations related to bladder cancer are dominantly acquired gene mutations and are not inherited. Previous comparative transcriptome analysis of urinary bladder cancer and control samples has revealed a set of genes that may play a role in tumor progression. Here we set out to investigate further the expression of two candidate genes, centromere protein U (CENPU) and mitochondrial ribosomal protein s28 (MRPS28) to better understand their role in bladder cancer pathogenesis. Our results confirmed that CENPU is up-regulated in human bladder cancer tissues at mRNA and protein levels. Gain-of-function and loss-of-function studies in T24 human urinary bladder cancer cell line revealed a hierarchical relationship between CENPU and MRPS28 in the regulation of cell viability, migration and invasion activity. CENPU expression was also up-regulated in in vivo nude mice xenograft model of bladder cancer and mice overexpressing CENPU had significantly higher tumor volume. In summary, our findings identify CENPU and MRPS28 in the molecular pathogenesis of bladder cancer and suggest that CENPU enhances the progression of bladder cancer by promoting MRPS28 expression.

• Applied Microscopy
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• 제19권2호
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• pp.119-137
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• 1989

Turtle bladder의 상피조직(上皮組織)과 세포막(細胞膜) 투과성(透過性)을 분석하기 위하여 동결절단법(凍結切斷法)을 적용(適用)하여 전자현미경(電子顯微鏡) 관찰을 하였으며 그 결과 다음과 같이 요약(要約)된다. 1. 방광상피(膀胱上皮)의 3가지 주요세포형(主要細胞形)은 granular-cell, ${\alpha}$ 및 ${\beta}$형(形)의 CA-rich cell로서 구분된다. 2. 과립성세포(顆粒性細胞)의 주요기능(主要機能)은 $Na^+$ 재흡수(再吸收)이며, 두 단계(段階)의 수송과정(輸送過程)으로 설명되며 정단세포막(頂端細胞膜)을 통한 $Na^+$의 세포내(細胞內) 확산이동(擴散移動)과 그후 기저막(基底膜)에 위치한 $Na^{+}\;-K^{+}$ 펌프에 의한 능동수송과정(能動輸送過程)이다. 3. ${\alpha}$ 및 ${\beta}$형(形) CA-rich cell은 $Na^+$ 수송(輸送)에 관여하지 않으며, ${\alpha}$형(形)의 CA-cell은 정단세포막(頂端細胞膜)의 proton펌프를 이용하여 proton 분필(分泌)에 관여한다. 또한 ${\beta}$형(形)의 CA-cell는 정단세포막(頂端細胞膜)을 통한 $HCO_{3}^-$ 분필수송(分泌輸送)의 기능을 가지고 있다. 4. 동결절단법(凍結切斷法)의 적용하에 세포막표면(細胞膜表面)의 특성(特性)을 관찰한 바, ${\alpha}$형(形)의 CA-cell의 정단세포막(頂端細胞膜)은 proton펌프를 함유하는 것으로 보이는 intramembrane particle이 다수 관찰되고 있으며, ${\beta}$형(形) CA-cell은 기저세포막(基底細胞膜)에서 이와같은 intramembrane particle이 나타나고 있다. 5. 상술(上述)한 두 type의 CA-cell에서 수송특성(輸送特性)의 차이는 proton 및 $HCO_{3}^-$ 분필수송(分泌輸送)이 서로 반대의 방향으로 일어나는 것으로 사료된다.