• Plant Disease and Agriculture
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• v.5 no.1
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• pp.64-66
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• 1999

Black leg occurred in potato (Solanum tuberosum L.) grown in Pyungchang, Kangwon-Do, Korea. The symptoms began as small water-soaked lesions on stem, and the affected stems discolored black to brown. It became yellow under dry condition. When lower parts of potato were affected in the field, wilting of leaves and desiccation of the stem were developed. The causal organism was isolated from lesions and identified as Erwinia carotovora subsp. atroseptica based on the morphological, physiological and biochemical characteristics. E. carotovora subsp. atroseptica is the first described bacterium which causes black leg in potato in Korea.

• Korean Journal Plant Pathology
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• v.14 no.6
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• pp.729-731
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• 1998

A black leg disease in wasabi occurred, showed black spots on the leaves, changed a rhizome color to black by invading the vascular bundles of stem and root, thus lowered the quality of the rhizome. The mycelium of the pathogen was yellow at first and then turned to dark yellow on oat meal agar medium. The pycnidium was globose or subglobose, dark brown in color, and 44~120$\times$28~170 ${\mu}{\textrm}{m}$ in size and had one or two ostioles on the upper part. The pycnidiospores are single-celled, hyaline, and 4~6$\times$1.2~2.3 ${\mu}{\textrm}{m}$ in size. The causal pathogen was identified as Phoma wasabiae. The black leg disease of wasabi occurred within the range of 28 to 32% at Chonbuk province in 1994~1995. The disease was appeared from April to October and severe in June and July. The black leg caused by P. wasabiae was first described in Korea.

• Korean Journal of Veterinary Research
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• v.4 no.1
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• pp.23-25
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• 1964

A comparative experiment on the duration of immunity and antigenicity of the Black-leg vaccine was conducted and following results were obtained. Animals vaccinated with formalin and modified living vaccine were equally survived when they were challenged 12 months after the vaccination. It was further found that the rate of survival was 50 % on both groups when the animals were challenged 16 months after the vaccination, and no distinctive difference was observed between the two groups by means of protection power.

• Korean Journal of Veterinary Research
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• v.8 no.2
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• pp.125-146
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• 1968

Throughout the studies the following experimental results were summarized. 1. It was impossible to infect and kill the mice, weighing 10 to 12 gm, by inoculating 0.2ml of virulent Cl. chauvoei, diluted 1 to 10 with physiological saline, via subcutaneous, intramuscular, intraperitoneal or intraveonus, route. 2. The mice which were inoculated in brain with 0.03ml of Cl. chauvoei diluted 1 : 5120 with physiological saline were resulted in all death after infection, but not in case of attenuated strain even in dilution of one to five. 3. Virulent Cl. chauvoei were diluted with each of those of whole blood, erythrocytes and serum of horse, calf, swine, sheep, rabbit, guinea pig, chicken and duck, human plasma and 2% CaCl solution, and inoculated subcutaneously 0.25 to 0.5ml in mice, weighing 12 to 15gm. It was resulted in significant increase in virulence as comparing with the case of physiological saline solution except when horse and pig sera were used. Such a phenomena were not seen in attenuated strain. 4. Virulence of virulent Cl. Chauvoei could be increased significantly in rat, as the procedures used in mice, by suspending in whole blood, erythrocytes, serum, or plasma of various animals, or 2% $CaCl_2$ solution and by inoculating subcutaneously 0.5 to 10ml in rat, weighing 30 to 60 gm, as compared with those of control group which used physiological saline solutionos diluent. 5. Mice resisted 100 and 80 percent against challenge of $10^3$ and $10^4$ M.L.D.. respectively, 24 hours after inoculation of 0.5ml black leg antiserum. 6. Immune response to the black leg living vaccine in mice could be obtained more favorably in the group of respected vaccination rather than those of single inoculation and the most profitable inoculm size of the vacine was 0.5 to 1.0ml. 7. Challenge for the immunized mice could be carried out effectively 3 weeks after first vaccination. 8. Satisfactory results could be obtained by inoculating subcutaneously for the immunization and intracerebrally or subcutaneously for the challenge. 9. Mice which were inoculated with 0.5ml of black leg living vaccine via subtaneucously two times at seven days interval and 21 days after first inoculation and challenged with 5 and 10 M.L.D. of virulent strain, resited 100 and 70 to 80 percent respectively. Same results were obtainable in black leg killed vaccine as the procedures used in living vaccine. 10. There were significantly different resistances against the definite challenge does between the mice groups which were immnuized with the living vaccine diluted five or 10 times and the undiluted. 11. For the biological assay of black leg living vaccine and antiserum, satisfactory results could be obtained using mice.

• Korean Journal of Veterinary Research
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• v.4 no.1
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• pp.27-33
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• 1964

In the treatment of mice Aureomycin, Oil penicillin, Achromycin, Ambrasynth and Terramycin were effective on the Black-leg disease when they were administered about 4 to 5 hours before the death of control animals. Synthomycetin, however, was observed to be effective when administered 8 hours or more before the control death. Streptomycin seemed to be rather less effective than Black leg antiserum. It was not effective at all when given 19 hours before the death of control animal although there was a delay of taking death by 5 hours in the treated group. As was in mice, Streptomycin was little effective in the treatment of calves, while other antibiotics showed fine efficacy on the diseased animals. The diseased animals, when given with anitibiotics 37 hours before the death of control showed the rise of temperature($41^{\circ}C$ or more), swelling and redness on the site of infection and then recovcred to normal stage. In the treatment of calves, Aureomycin only delayed the taking death of the deseased animals by 111 hours when administered 4 hours before the death of control animals. And other antibiotics, when given 4 hours before the death of control animals were effective, although the deseased animls developed symptoms such as hard breathing, anorexia and dropsical swellings. Oil penicillin was observed to be comparatively larger in its efficacy on the treatment of Black leg disease.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.8
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• pp.1201-1206
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• 2008

The effects of free-range farming, compared to a conventional production system, on carcass and meat qualities were studied using black-feathered Taiwan native chickens. Twenty 16-week old females were purchased separately from a free-range farm and a conventional production farm and used for this study. The results showed similarities in the live weight (roughly 2.1 kg), dressing percentage (69%) and meat percentage (19%) of deboned leg quarter. Significant differences (p<0.05) found for the free-range chickens included: a higher percentage of meat for the breast, an increased crude protein content and chewiness value for the breast, but decreased crude fat content and lower hardness and fracturablility values for the leg quarter. Significantly higher L* values were found for the breast and leg meat of conventionally produced chickens, whereas no significant differences were found for WHC and purge loss between the breast and the leg, and between the two production systems as well. Results of sensory evaluation showed a significant preference for leg over breast meat (p<0.05). The scores of all the attributes including aroma, flavor, firmness, tenderness, juiciness and overall acceptability of leg meat from free-range chickens were slightly higher than for conventional chickens, while the reverse was true for breast meat, though no significant difference could be found. Free-range Taiwan native chicken appeared to yield the best of the results, with flavorful yet tender leg meat for higher sensory satisfaction, and high-protein but low-fat breast meat for healthier diet choice.

• Journal of Bio-Environment Control
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• v.12 no.4
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• pp.180-183
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• 2003

P. wasabiae was isolated from discolored seeds of wasabi(Wasabia japonical Mtsum.) and inoculated to fresh seeds, then the effect of fungicides on suppression of diseases were determined. Emergence rate of wasabi seeds where suppressed to 52.5% by the inoculation and it reached up to 92.7% by dipping treatment of inoculated seeds ito benomyl solution. The incidence rate of black leg disease and damping off were 32.0 and 22.0%, respectively, in control treatment that sown in the soil inoculated with P. wasabiae. But dipping treatment of inoculated seeds into benomyl solution resulted in 12.0% and 10.7% in incidence rate of those two diseases, respectively.

• Animal Bioscience
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• v.36 no.2
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• pp.191-199
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• 2023

Objective: This study aimed to investigate the significant single nucleotide polymorphisms (SNPs) and genes associated with nine reproduction and morphological traits in three breed populations of Chinese goats. Methods: The genome-wide association of nine reproduction and morphological traits (litter size, nipple number, wattle, skin color, coat color, black dorsal line, beard, beard length, and hind leg hair) were analyzed in three Chinese native goat breeds (n = 336) using an Illumina Goat SNP50 Beadchip. Results: A total of 17 genome-wide or chromosome-wide significant SNPs associated with one reproduction trait (litter size) and six morphological traits (wattle, coat color, black dorsal line, beard, beard length, and hind leg hair) were identified in three Chinese native goat breeds, and the candidate genes were annotated. The significant SNPs and corresponding putative candidate genes for each trait are as follows: two SNPs located on chromosomes 6 (CSN3) and 24 (TCF4) for litter size trait; two SNPs located on chromosome 9 (KATNA1) and 1 (UBASH3A) for wattle trait; three SNPs located on chromosome 26 (SORCS3), 24 (DYM), and 20 (PDE4D) for coat color trait; two SNPs located on chromosome 18 (TCF25) and 15 (CLMP) for black dorsal line trait; four SNPs located on chromosome 8, 2 (PAX3), 5 (PIK3C2G), and 28 (PLA2G12B and OIT3) for beard trait; one SNP located on chromosome 18 (KCNG4) for beard length trait; three SNPs located on chromosome 17 (GLRB and GRIA2), 28 (PGBD5), and 4 for hind leg hair trait. In contrast, there were no SNPs identified for nipple number and skin color. Conclusion: The significant SNPs or genes identified in this study provided novel insights into the genetic mechanism underlying important reproduction and morphological traits of three local goat breeds in Southern China as well as further potential applications for breeding goats.

• Journal of the Korea Furniture Society
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• v.16 no.2 s.30
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• pp.59-65
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• 2005

As physical condition of students improves, there is a need to develop human body-friendly desk and chair for students. In this study, desks and chairs were manufactured with curved veneer lamination under high frequency heating and pressing, using ten wood species such as Japanese red pine, Korean pine, pitch pine, Japanese larch, yellow poplar, black locust, oak, radiata pine, beech, and birch. The performance of these products were evaluated. The results obtained were summarized as follows; With high frequency heating, the turned lamination of veneers with full size sheet ($3{\times}6\;feet$) prepared by rotary lathe peeling was successfully applied for making the members of desk top, leg frames of desk and chair. Bending strengths of desk tops were relatively greater for yellow poplar, black locust and red pine, which were similar to those of beech and birch. Bending strengths of desk legs were classified into greater species group (red pine, yellow poplar, larch) and lower species group (radiata pine, Korean pine, pitch pine). Compressive strengths of chair legs in parallel direction to the lamination were greater in black locust and larch. On the other hand, differences between outer and inner gap at the top and drawer bottom of desk top were rather larger for the laminations of birch and beech, and less for those of yellow poplar and pitch pine, showing greater stability of open drawer space. In results, yellow poplar, larch, pitch pine and red pine showed good appearance and strength properties at the curved veneer lamination. Accordingly, it was believed that these domestic woods were able to substitute for birch which was being imported for the use of veneer-laminates type furniture.

• Korean Journal of Agricultural Science
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• v.49 no.2
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• pp.249-257
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• 2022

The present study investigates the effects of different inclusion levels of a microwave-dried black soldier fly (Hermetia illucens) larvae meal (HILM) on the carcass characteristics, breast meat quality, and fatty acid profiles of leg meat in broiler chickens. A total of 150 male broilers were randomly assigned to three dietary treatment groups (10 replicates·treatment^-1 and 5 birds·replicate^-1). HILM was included at increasing levels (0, 2, and 4%; CON, 2HILM, and 4HILM, respectively) in diets formulated for three feeding phases: starter (1 - 7 d), grower (7 - 21 d), and finisher (21 - 35 d). One bird per replicate was slaughtered at d 35; the carcasses and organs were weighed, and breast and leg meats were excised for a meat analysis. A linear decrease (p < 0.01) was observed for live and carcass weights for the whole period of the experiment, with no difference between the CON and 2HILM groups. Broilers fed HILM showed no significant differences in meat quality parameters, except for an increase in yellowness (b^*) in the 2HILM and 4HILM groups compared to the CON group (p < 0.01). With an increase in the HILM inclusion level, the proportion of saturated fatty acids (SFAs) such as lauric, myristic, stearic, arachidic, and heneicosylic acids showed a linear increase (p < 0.01); however, total SFA, monounsaturated fatty acid, and polyunsaturated fatty acid did not differ among the dietary treatments. Thus, microwave-dried HILM can be considered as a possible alternative ingredient to soybean meal in broiler diets up to a 2% inclusion level.