• Title/Summary/Keyword: biovar 3

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Physiological and Biochemical Properties of Isolates of Rhizobia from Soybean (콩에서 분리한 근류균의 생리, 생화학적 특성)

  • 박기선;최재을
    • Korean Journal Plant Pathology
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    • v.12 no.3
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    • pp.351-357
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    • 1996
  • 콩으로부터 분리한 140균주의 근류균은 25균주(17.9%)가 Rhizobium fredii로 115 균주(82.1%)가 Brady-rhizobium japonicum으로 동정되었다. R. fredii에 속하는 분리 균주의 생존 pH 범위는 4..5∼9.0이었고 B. japonicum의 생육 pH 범위는 5.5∼8.0로 비교적 좁게 나타났다. B. japonicum에 속하는 98균주 중에서는 53균주(54%)가 IAA를 생산하지 않는 GT I group으로, 45균주(46%)는 IAA를 생산하는 GT II group으로 명확하게 구분되었으며, 항생물질에 대한 내성 유무에 의해 10개의 group으로 구분되었다.

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A Proposed Manual for the Efficient Management of Kiwifruit Bacterial Canker in Korea (키위 궤양병 효율적 관리를 위한 매뉴얼)

  • Koh, Young Jin;Kim, Gyoung Hee;Jung, Jae Sung
    • Research in Plant Disease
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    • v.23 no.1
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    • pp.1-18
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    • 2017
  • Pseudomonas syringae pv. actinidiae, the causal agent of bacterial canker, is currently causing severe economic losses to kiwifruit production worldwide. The pathogen has affected green-fleshed kiwifruit cutlivars and yellow-fleshed kiwifruit cultivars since 1988 and 2006 in Korea, respectively. In recent years, the biovar 3 strains of P. syringae pv. actinidiae were introduced through imported contaminated pollens and have rapidly spread to neighboring kiwiruit orchards by secondary infection, leading to outbreaks of bacterial canker and tremendous damages on yellow- and red-fleshed kiwifruit cultivars. In this review, we summarize the various management practices of bacterial canker of kiwifruit such as disease escaping, cultural practices, blocking of dissemination, early diagnosis, eradication of inoculum sources, chemical control, and trunk injection on the basis of our research works and field experiences and important research products conducted during the last three decades in the world. Finally, we propose a manual for the efficient management of the disease that can be practically utilized at the farmers' orchards in order to keep kiwifruit vines healthy in the future.

Identification of Agrobacterium tumefaciens from Soil and Transformation of Maize (토양으로부터의 Agrobacterium tumefaciens의 분리, 동정 및 옥수수의 형질전환에 이용)

  • 노광수;강봉중
    • KSBB Journal
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    • v.7 no.3
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    • pp.191-200
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    • 1992
  • Several strains of Agrobacterium tumefaciens were isolated from soil in the Taegu area and characterized to develop some useful vector systems for higher plant genetic engineering. The selected colonies had a unique form, and strains from the colonies were capable of tumor formation on the sunflower leaf surface. They had a large plasmid. The restriction analysis showed that they were another kinds of Ti plasmic compared with C58 and Ach5. The isolated strains were identified as the nopaline type and also as biovar 1 A. tumefaciens, according to their tumor morphology, blophyslcal and biochemical characteristics. One of the isolated strains, AK204 was transformed with binary vector (pGA642), having selectable marker (Kmr, Tcr). Furthermore, maize tissue cells were transformed by cocultivation with AK204/pGA642, and the transformants were selected on the selective medium and identified using PAGE patterns of their soluble proteins.

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Studies on the fluorescent Pseudomoηas isolated from the wheat rhizosphere (소맥근권(小麥根圈)에서 분리(分離)한 형광성(螢光性) Pseudomonas spp. 에 관(關)한 연구(硏究))

  • Lee, Myung-Chol;Kim, Yong-Woong;Kim, Kwang-Sik
    • Korean Journal of Soil Science and Fertilizer
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    • v.23 no.2
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    • pp.152-159
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    • 1990
  • Four active strains, producing siderophore and antagonizing to soil plant disease fungi, were isolated and identified from the wheat rhizosphere to reduce the injurious effects of continuous cropping and study on the biological control. The obtained results were summerized as follow. 1. Four strains of fluorescent Pseudomonas were isolated from the wheat rhizosphere and identified as Pseudomonas fluorescens Biotype A(Ps-1,2,5) and Biotype B(Ps-3) That strains inhibitied growth of R. solani, F.oxysperum and F. solani in vitro 2. Optical density of pigment was maximum at 410nm. 3. Siderophore production by identified strains was decreased with addition of $Fe^{+3}$, although not decreased with addition of $Fe^{+2}$ 4. Pigment of Ps-1, 2 and 3 strain inhibitied growth of R. slani, F. oxysperum and F. solani but pigment of Ps-5 strain did not inhibitie growth of R. solani 5. Effect of inoculation was in order of Ps-2, 1, 5 and 3 strain through the dark culture method, and effect of Ps-1 and Ps-2 was greater than that of treatment of captan 50ppm.

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Biovars and serovars of Pasteurella haemolytica isolated from pneumonic calves and goats (호흡기 증상을 나타낸 송아지 및 산양에서 분리한 Pasteurella haemolytica의 생물형 및 혈청형)

  • Cho, Kwang-hyun;Kim, Bong-hwan
    • Korean Journal of Veterinary Research
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    • v.32 no.1
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    • pp.51-55
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    • 1992
  • The biovars and serovars of 57 strains of Pasteurella haemolytica(P haemolytica) isolated from pneumonic calves and goats were investigated. The biovars were determined some biochemical and cultural properties and susceptibility to penicillin. All 57 isolates were considered to correspond to biovar A. Among 57 P haemolytica, 39 isolates of them(68.4%) were serovar 1.2(3.5%) were serovar 5 and 2(3.5%) were serovar 7, the remaining 14 isolates(24.6%) were untypable.

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Identification of a Prophage-encoded Abortive Infection System in Levilactobacillus brevis

  • Feyereisen, Marine;Mahony, Jennifer;O'Sullivan, Tadhg;Boer, Viktor;van Sinderen, Douwe
    • Microbiology and Biotechnology Letters
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    • v.48 no.3
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    • pp.322-327
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    • 2020
  • Abortive infection systems (Abi) are phage resistance systems that can be prophage-encoded. Here, two genes encoding an Abi system were identified on a prophage sequence contained by the chromosome of the Levilactobacillus brevis strain UCCLBBS124. This Abi system is similar to the two-component AbiL system encoded by Lactococcus lactis biovar. diacetylactis LD10-1. The UCCLBBS124 prophage-derived Abi system (designated here as AbiL124) was shown to exhibit specific activity against phages infecting L. brevis and L. lactis strains. Expression of the AbiL124 system was shown to cause reduction in the efficiency of plaquing and cell lysis delay for phages of both species.

Anticoagulant Activity of Sulfoakyl Derivatives of Curdlan

  • Lee, Kyung-Bok;Bae, Jong-Hwan;Kim, Jong-Seung;Yoo, Yung-Choon;Kim, Beom-Soo;Kwak, Sang-Tae;Kim, Yeong-Shik
    • Archives of Pharmacal Research
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    • v.24 no.2
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    • pp.109-113
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    • 2001
  • Curdlan is a natural $\beta$-1,3-glucan produced by Agrobacterium biovar 1. In this study, the anticoagulant activity of sulfoalkyl derivatives of curdlan was investigated by carrying out activated partial thromboplastin time (APTT) assay and compared with that of o-sulfonated curdlan. Approximately 100-fold higher concentration of o-sulfonated curdlan than heparin was required to obtain the same level of the clotting time. Anticoagulant activity of curdlan derivatives was dependent on the degree of sulfation in prolonging the clotting time. However, the chain length of the substituent did not play a role in prolonging the clotting time. The curdlan derivatives enhanced thrombin inhibition by mediating through antithrombin III. The inhibition of thrombin by o-sulfonated curdlan was found to be approximately 10-fold weaker than that by heparin.

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DNA fingerprinting of Brucella abortus isolated from bovine brucellosis outbreaks by repetitive element sequence (rep)-PCR

  • Suh, Dong Kyun
    • Korean Journal of Veterinary Research
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    • v.45 no.2
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    • pp.199-205
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    • 2005
  • DNA fingerprint patterns of 8 Brucella reference strains and 15 B. abortus field isolates were characterized by repetitive element sequence-based PCR (rep-PCR) using BOX- and ERIC-primers in this study. AMOS PCR differentiated all Brucella field isolates from B. abortus RB51, a vaccine strain by producing a B. abortus-specific 498 bp band. Rep-PCR using BOX-primer produced 13 to 18 bands with sizes of between 230 and 3,300 bp, and discriminated Brucella strains to the species level except B. canis and B. suis. PCR products amplified with ERIC primers were, however, not appropriate for differentiating the Brucella isolates. DNA fingerprint patterns for all B. abortus field isolates were identical among them and were put on one cluster with B. abortus biovar 1 reference strain in the dendrogram, indicating they were highly clonal. These results suggested that rep-PCR using BOX primer might to be a useful tool for calculating genetic relatedness among the Brucella species and for the study of brucellosis epidemiology.

Mortality of the Horned Turban Shell, Batillus cornutus Caused by Vibrio spp (소라(Batillus cornutus)의 비브리오균 감염에 의한 폐사)

  • 이정재;허문수
    • The Korean Journal of Malacology
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    • v.15 no.1
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    • pp.49-55
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    • 1999
  • Mass mortality of the horned turban shell, Batillus cornutus was investigated using histological and bacteriological methods. Some pathogenic bacteria were isolated from mortal or inactive individuals. The pathogenic agents causing mortality of the horned turban shells were as Vibrio alginolyticus and V. anguillarum. Laboratory experiment indicated that optimal growth temperature of two bacteria was 25 to 30$^{\circ}C$ and 3% of NaCl. Histological examination of the horned turban shells showed that gill necrosis is one of the major symptom of infected individuals. It was believed that sudden increase of those two bacterial agents due to environmental change cause mortality of horned turban shells.

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Isolation, identification and serological investigation of Actinobacillus pleuropneumoniae in slaughtered pigs (도축돈에서의 Actinobacillus pleuropneumoniae 분리, 동정 및 감염률 조사)

  • Kim, Kyung-Eon;Ku, Kyung-Nyer;Ko, Jae-Hyung;Moon, Hyeong-Jun;Choi, Kwon-Rag;Song, Eun-Ah;Park, Mi-Young
    • Korean Journal of Veterinary Service
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    • v.36 no.3
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    • pp.181-186
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    • 2013
  • This study was conducted to isolate the Actinobacillus pleuropneumoniae (APP) and to find out the distribution of 15 serovars mainly in southern Gyeonggi province, Korea. From July 2011 to Nov. 2012, a total of 2,204 slaughter pigs (110 herds) were inspected for evaluation of APP like pneumonic lesions. 48 (33.8%) APP strains were isolated from the 142 lungs and identified using PCR assays (cps, apx/omlA, biovar). Consequently, the serotype ratio were as in the following; type2 41.7% (n=20), type5 33.3% (n=16), type12 10.4% (n=5), type1 6.2% (n=3), type4 and 7 2.1% (n=1) and unknown 4.2% (n=2). Also serological test was implemented for 452 (83 herds) serum samples randomly collected from above slaughter pigs using commercial ELISA kits. The positive ratio of each serotype for tested pigs were 19.1% (77/404) on [2], 7.1% (32/452) on [3, 6, 8], 6.9% (28/404) on [5a, 5b], 6.2% (28/452) on [4, 7], 2.8% (9/320) on [12], 2.0% (9/452) on [1, 9, 11] and 0.0% (0/452) on [10]. And 49.3% (223/452) of pigs were positive on apxIV antibody. On the basis of latter screening test, the infected farm ratio accounted for 71.1% (59/83) and that was much higher than previously reported data.