• 제목/요약/키워드: biotechnology and medical engineering

검색결과 347건 처리시간 0.028초

Siderophore Production by Rhizosphere Biological Control Bacteria Brevibacillus brevis GZDF3 of Pinellia ternata and Its Antifungal Effects on Candida albicans

  • Sheng, Miaomiao;Jia, Huake;Zhang, Gongyou;Zeng, Lina;Zhang, Tingting;Long, Yaohang;Lan, Jing;Hu, Zuquan;Zeng, Zhu;Wang, Bing;Liu, Hongmei
    • Journal of Microbiology and Biotechnology
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    • 제30권5호
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    • pp.689-699
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    • 2020
  • Brevibacillus brevis GZDF3 is a gram-positive, plant growth-promoting rhizosphere bacterium (PGPR) isolated from the rhizosphere soil of Pinellia ternata (an important herb in traditional Chinese medicine). The GZDF3 strain produces certain active compounds, such as siderophores, which are the final metabolite products of non-ribosomal peptide synthetase (NRPS) and independent non-ribosomal peptide synthetase (NIS) activity. With the present study, we attempted to investigate the siderophore production characteristics and conditions of Bacillus sp. GZDF3. The antibacterial activity of the siderophores on pathogenic fungi was also investigated. Optimal conditions for the synthesis of siderophores were determined by single factor method, using sucrose 15 g/l, asparagine 2 g/l, 32℃, and 48 h. The optimized sucrose asparagine medium significantly increased the production of siderophores, from 27.09% to 54.99%. Moreover, the effects of different kinds of metal ions on siderophore production were explored here. We found that Fe3+ and Cu2+ significantly inhibited the synthesis of siderophores. The preliminary separation and purification of siderophores by immobilized-metal affinity chromatography (IMAC) provides strong antibacterial activity against Candida albicans. The synergistic effect of siderophores and amphotericin B was also demonstrated. Our results have shown that the GZDF3 strain could produce a large amount of siderophores with strong antagonistic activity, which is helpful in the development of new biological control agents.

Evaluation of Gene Expression Changes of Serotonin Receptors, 5-HT3AR and 5-HT2AR as Main Stress Factors in Breast Cancer Patients

  • Hejazi, Seyed Hesam;Ahangari, Ghasem;Pornour, Majid;Deezagi, Abdolkhaleagh;Aminzadeh, Saeed;Ahmadkhaniha, Hamid Reza;Akbari, Mohamad Esmail
    • Asian Pacific Journal of Cancer Prevention
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    • 제15권11호
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    • pp.4455-4458
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    • 2014
  • Breast cancer is a serious and potentially lethal multi-factor disease among 40-50 aged women in both developed and developing countries. Also, various studies have pointed to roles of neurotransmitters like serotonin in development of cancers, through action on various types of receptors. This study was conducted to evaluate serotonin receptor (5HT2AR and 5HT3AR) genes expression in peripheral blood mononuclear cells (PBMCs) of breast cancer patients in comparison with the healthy people and in the MCF7 cell line. Peripheral blood samples were obtained from 30 patients and 30 healthy individuals. Total RNA was extracted from PBMCs and MCF-7 cells. and 5HT2AR and 5HT3AR were detected by RT-PCR techniques. Finally, serotonin receptor gene expression variation in breast cancer patients and MCF-7 cells were determined by real time-PCR. This latter indicated significant promotion in expression of 5HT3AR and 5HT2AR in PBMCs in breast cancer patients but expression of 5HT2AR in the MCF-7 cell line was significantly decreased. In conclusion, after performing complimentary tests, determine of gene expression changes in serotonin receptors (5HT2AR and 5HT3AR) may be useful as a new approach in treatment of breast cancer based on use of antagonists.

방기의 품질 평가를 위한 Sinomenine, Magnoflorine, Syringaresinol의 함량 분석 (Quantitative Analysis for the Quality Evaluation of Sinomenine, Magnoflorine and Syringaresinol in Sinomenium acutum)

  • 이지우;원진배;윤보라;엄민례;마충제
    • 약학회지
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    • 제57권3호
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    • pp.161-166
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    • 2013
  • Sinomenium acutum has been used for the treatment of rheumatoid arthritis, arrhythmia, and pain. We evaluated the quality of S. acutum extract by quantitatively analyzing its components such as sinomenine, magnoflorine and syringaresinol with the simultaneous determination method using HPLC-DAD. A total of 53 samples collected from different localities were evaluated with this quality evaluation method. Sinomenine, magnoflorine and syringaresinol from tested samples ranged from 0.0649~9.1050%, 0.7460~10.7590% and 0.0010~0.2441%, respectively. In the current study, we were able to exhibit the diverse quality of S. acutum samples collected from various locations using the simultaneous determination method.

Designing Signal Peptides for Efficient Periplasmic Expression of Human Growth Hormone in Escherichia coli

  • Jeiranikhameneh, Meisam;Moshiri, Farzaneh;Falasafi, Soheil Keyhan;Zomorodipour, Alireza
    • Journal of Microbiology and Biotechnology
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    • 제27권11호
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    • pp.1999-2009
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    • 2017
  • The secretion efficiency of a protein in a Sec-type secretion system is mainly determined by an N-terminal signal peptide and its combination with its cognate protein. Five signal peptides, namely, two synthetic Sec-type and three Bacillus licheniformis alpha-amylase-derived signal peptides, were compared for periplasmic expression of the human growth hormone (hGH) in E. coli. Based on in silico predictions on the signal peptides' cleavage efficiencies and their corresponding mRNA secondary structures, a number of amino acid substitutions and silent mutations were considered in the modified signal sequences. The two synthetic signal peptides, specifically designed for hGH secretion in E. coli, differ in their N-terminal positively charged residues and hydrophobic region lengths. According to the mRNA secondary structure predictions, combinations of the protein and each of the five signal sequences could lead to different outcomes, especially when accessibility of the initiator ATG and ribosome binding sites were considered. In the experimental stage, the two synthetic signal peptides displayed complete processing and resulted in efficient secretion of the mature hGH in periplasmic regions, as was demonstrated by protein analysis. The three alpha-amylase-derived signal peptides, however, were processed partially from their precursors. Therefore, to achieve efficient secretion of a protein in a heterologous system, designing a specific signal peptide by using a combined approach of optimizations of the mRNA secondary structure and the signal peptide H-domain and cleavage site is recommended.

Production of Transgenic Mice Secreting a C. thermocellum Cellulase D in the Pancreas

  • Park, Jung-Ok;Lee, Eun-Ju;Kim, Myoung-Ok;Kim, Sung-Hyun;Park, Jun-Hong;Cho, Kyung-In;Nam, Myung-Su;Park, Hum-Dai;Ryoo, Zae-Young
    • 한국동물번식학회:학술대회논문집
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    • 한국동물번식학회 2002년도 춘계학술발표대회 발표논문초록집
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    • pp.94-94
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    • 2002
  • Increasing competition in the livestock industry has forced producers to cut costs by adopting new technologies aimed out increasing production efficiency. Non-ruminant livestock do not express fibrolytic enzymes. The major plant cell wall components of cereals, primarily β-glucans and arabinoxylans, form gel-like structures in the small intestines that trap nutrients. The viscous polysaccharides can also cause severe gastrointestinal disorders. (omitted)

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The CRISPR Growth Spurt: from Bench to Clinic on Versatile Small RNAs

  • Bayat, Hadi;Omidi, Meysam;Rajabibazl, Masoumeh;Sabri, Suriana;Rahimpour, Azam
    • Journal of Microbiology and Biotechnology
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    • 제27권2호
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    • pp.207-218
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    • 2017
  • Clustered regulatory interspaced short palindromic repeats (CRISPR) in association with CRISPR-associated protein (Cas) is an adaptive immune system, playing a pivotal role in the defense of bacteria and archaea. Ease of handling and cost effectiveness make the CRISPR-Cas system an ideal programmable nuclease tool. Recent advances in understanding the CRISPR-Cas system have tremendously improved its efficiency. For instance, it is possible to recapitulate the chronicle CRISPR-Cas from its infancy and inaugurate a developed version by generating novel variants of Cas proteins, subduing off-target effects, and optimizing of innovative strategies. In summary, the CRISPR-Cas system could be employed in a number of applications, including providing model systems, rectification of detrimental mutations, and antiviral therapies.

Methods for rapid identification of a functional single-chain variable fragment using alkaline phosphatase fusion

  • Lee, Kyung-Woo;Hur, Byung-Ung;Song, Suk-Yoon;Choi, Hyo-Jung;Shin, Sang-Hoon;Cha, Sang-Hoon
    • BMB Reports
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    • 제42권11호
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    • pp.731-736
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    • 2009
  • The generation of functional recombinant antibodies from hybridomas is necessary for antibody engineering. However, this is not easily accomplished due to high levels of aberrant heavy and light chain mRNAs, which require a highly selective technology that has proven complicated and difficult to operate. Herein, we attempt to use an alkaline phosphate (AP)-fused form of single-chain variable fragment (scFv) for the simple identification of a hybridoma-derived, functional recombinant antibody. As a representative example, we cloned the scFv gene from a hybridoma-producing mouse IgG against branched-chain keto acid dehydrogenase complex-E2 (BCKD-E2) into an expression vector containing an in-frame phoA gene. Functional recombinant antibodies were easily identified by conventional enzyme-linked immunosorbent assay (ELISA) by employing scFv-AP fusion protein, which also readily serves as a valuable immuno-detective reagent.

빅데이터 활용 의학·바이오 부문 사업화 가능 기술 연구 (Research on the development of demand for medical and bio technology using big data)

  • 이봉문;남가영;강병철;김치용
    • 한국멀티미디어학회논문지
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    • 제25권2호
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    • pp.345-352
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    • 2022
  • Conducting AI-based fusion business due to the increment of ICT fusion medical device has been expanded. In addition, AI-based medical devices help change existing medical system on treatment into the paradigm of customized treatment such as preliminary diagnosis and prevention. It will be generally promoted to the change of medical device industry. Although the current demand forecasting of medical biotechnology commercialization is based on the method of Delphi and AHP, there is a problem that it is difficult to have a generalization due to fluctuation results according to a pool of participants. Therefore, the purpose of the paper is to predict demand forecasting for identifying promising technology based on building up big data in medical biotechnology. The development method is to employ candidate technologies of keywords extracted from SCOPUS and to use word2vec for drawing analysis indicator, technological distance similarity, and recommended technological similarity of top-level items in order to achieve a reasonable result. In addition, the method builds up academic big data for 5 years (2016-2020) in order to commercialize technology excavation on demand perspective. Lastly, the paper employs global data studies in order to develop domestic and international demand for technology excavation in the medical biotechnology field.

Selective Sterilization of Vibro parahaemolyticus from a Bacterial Mixture by Low-Amperage Electric Current

  • Jin, Soo-Chang;Yoo, Hyun-Suk;Woo, Yeon-I.;Lee, Mi-Hee;Vagaska, Barbora;Kim, Jung-Sung;Uzawa, Masakazu;Park, Jong-Chul
    • Journal of Microbiology and Biotechnology
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    • 제19권6호
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    • pp.537-541
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    • 2009
  • The objective of this study was to investigate the possibility of using low-amperage electrical treatment (LAET) as a selective bacteriocide. Mixtures containing Escherichia coli, Staphylococcus aureus, and Vibrio parahaemolyticus were treated with different electric current intensities and for different times. The results showed that at 263 mA, treating bacteria for 100 ms eliminated all V. parahaemolyticus colonies. Although LAET reduced the populations of the three microorganisms, V. parahaemolyticus was more injured by LAET than S. aureus and E. coli when treated at the same processing conditions.