• Korean Journal of Soil Science and Fertilizer
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• v.46 no.2
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• pp.81-86
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• 2013

For organic farming, green manure crops such as leguminous forages and barley have been broadly used to improve soil fertility and soil physical and chemical properties by repeatedly cutting and mulching them directly as winter crop in the field in the rotation. In this investigation we selected 78 agricultural farm corporations as well as individual organic farmhouses related to crop rotation from greenmanure crops to main crops in order to analyze the relationship of cropping system between main crops and green manure crops. The results showed that the green manure crops were divided into two groups as leguminous and nonleguminous crops, representing that those are limited to specific climate and farming systems of regions. Also the 10 or less green manure crops including sudangrass, hairyvetch, italian ryegrass, sorghun, buckwheat, oat, pea, rye, clover, and canola which belong to leguminous crops which are presently cultivated from the organic farmhouses within the rotational crop system. We also confirmed that the major main crops are sweet potato, soybean, corn, tobacco, spinach from usage frequency analyzed by NetMiner H 2.6 which was used to estimate the rotational cropping system among the green manure crops and main crops.

• The Plant Pathology Journal
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• v.38 no.3
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• pp.212-219
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• 2022

Fusarium root rot caused by the soil-borne fungus Fusarium solani is one of the most important fungal diseases of cassava in Thailand, resulting in high yield losses of more than 80%. This study aimed to investigate if the exogenous application of salicylic acid formulations (Zacha) can induce resistance in cassava against Fusarium root rot and observe the biochemical changes in induced cassava leaf tissues through synchrotron radiation based on Fourier-transform infrared (SR-FTIR) microspectroscopy. We demonstrated that the application of Zacha11 prototype formulations could induce resistance against Fusarium root rot in cassava. The in vitro experimental results showed that Zacha11 prototype formulations inhibited the growth of F. solani at approximately 34.83%. Furthermore, a significant reduction in the disease severity of Fusarium root rot disease at 60 days after challenge inoculation was observed in cassava plants treated with Zacha11 at a concentration of 500 ppm (9.0%). Population densities of F. solani were determined at 7 days after inoculation. Treatment of the Zacha11 at a concentration of 500 ppm resulted in reduced populations compared with the distilled water control and differences among treatment means at each assay date. Moreover, the SR-FTIR spectral changes of Zacha11-treated epidermal tissues of leaves had higher integral areas of lipids, lignins, and pectins (1,770-1,700/cm), amide I (1,700-1,600/cm), amide II (1,600-1,500/cm), hemicellulose, lignin (1,300-1,200/cm), and cellulose (1,155/cm). Therefore, alteration in defensive carbohydrates, lipids, and proteins contributed to generate barriers against Fusarium invasion in cassava roots, leading to lower the root rot disease severity.

• Microbiology and Biotechnology Letters
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• v.51 no.1
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• pp.83-92
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• 2023

In this study, bioconversion of rutin to quercetin was confirmed by the fermentation of Korean indigenous probiotics and tartary buckwheat. Based on whole genome sequencing of 17 probiotics species, α-rhamnosidase, related to bioconversion of isoquercetin (quercetin 3-β-D glucoside) from rutin, is identified in the genome of CBT BG7, LC5, LR5, LP3, LA1, and LGA1. β-Glucosidase, related to bioconversion of isoquercetin to quercetin, is identified in the genome of all 17 species. Among the 17 probiotics species, 6 probiotics including CBT BG7, LR5, LP3, LA1, LGA1 and ST3 performed the bioconversion of rutin to quercetin up to 21.5 ± 0.3% at 7 days after fermentation. The fermentation of each probiotics together with enzyme complex Cellulase KN^® was conducted to reduce the time of bioconversion. As a result, CBT LA1 which showed the highest yield of bioconversion of 21.5 ± 0.3% when the enzyme complex was not added showed high bioconversion yield of 84.6 ± 0.5% with adding the enzyme complex at 1 day after fermentation. In particular, CBT ST3 (96.2 ± 0.4%), SL6 (90.1 ± 1.4%) and LP3 (90.0 ± 0.4%) showed high yield of bioconversion more than 90%. In addition, such probiotics including high levels in quercetin indicated the inhibitory effects of NO production in LPS-induced RAW264.7 cells. In this study, we confirmed that the fermentation of Korean indigenous probiotics and enzyme complex together with roasted tartary buckwheat increased the content of quercetin and reduced the time of bioconversion of rutin to quercetin which is a bioactive compound related to anti-inflammatory, antioxidants, anti-obesity, and anti-diabetes.

• International Journal of Industrial Entomology and Biomaterials
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• v.15 no.2
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• pp.145-155
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• 2007

Mulberry (Morus spp.) is an economically important tree crop being cultivated in India, China and other sericulturally important countries for its foliage to feed the silk producing insect Bombyx mori L. Genetic improvements of mulberry lag behind to the same in many other economically less important crops due to the complexity of its genetics, the breeding behavior, and the lack of basic information on factors governing important agronomic traits. In this review, the general usage and advantages of different molecular markers including isoenzymes, RFLPs, RAPDs, ISSRs, SSRs, AFLPs and SNPs are described to enlighten their applicability in mulberry genetic improvement programs. Application of DNA markers in germplasm characterization, construction of genetic linkage maps, QTL identification and in marker-assisted selection was also described along with its present status and future prospects.

• 한국약용작물학회:학술대회논문집
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• 2012.05a
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• pp.267-268
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• 2012

• 한국약용작물학회:학술대회논문집
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• 2012.05a
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• pp.265-266
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• 2012

• International Journal of Industrial Entomology and Biomaterials
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• v.14 no.2
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• pp.99-105
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• 2007

The success of rearing with presently available conventional bivoltine is unpredictable in some seasons of the tropical regions due to highly fluctuating adverse climatic conditions. Thus, in order to popularize bivoltine breeds in tropical parts of India, it is very much essential to have a bivoltine breed(s), which can give stable cocoon crop under variable environments. With this objective a breeding programme was undertaken to improve the survival trait in bivoltine silkworm by introducing multivoltine genes into bivoltine through back crossing. Resultant bivoltine lines showed significantly higher survival in compared to the receptor (Bivoltine) parent and control bivoltine breed. Esterase isozyme analysis revealed similar banding pattern in the developed bivoltine and in the donor multivoltine, which predicts the introgression of multivoltine character into evolved bivoltine.

• The Plant Pathology Journal
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• v.35 no.3
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• pp.280-286
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• 2019

In this study, PVF11 was selected among 20 candidate pathogenesis-related genes in Burkholderia glumae based on its effect on virulence to rice. PVF11 was found to interact with several plant defense-related WRKY proteins as evidenced through yeast-two hybrid analysis (Y2H). Moreover, PVF11 showed interactions with abiotic and biotic stress response-related rice proteins, as shown by genome-wide Y2H screening employing PVF11 and a cDNA library from B. glumae-infected rice. To confirm the effect of PVF11 on B. glumae virulence, in planta assays were conducted at different stages of rice growth. As a result, a PVF11-defective mutant showed reduced virulence in rice seedlings and stems but not in rice panicles, indicating that PVF11 involvement in B. glumae virulence in rice is stage-dependent.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.207-207
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• 2022

Eating and cooking quality (ECQ) is one of the most complex quantitative traits in rice. The understanding of genetic regulation of transcript expression levels attributing to phenotypic variation in ECQ traits is limited. We integrated whole-genome resequencing, transcriptome, and phenotypic variation data from 84 Japonica accessions to build a transcriptome-wide association study (TWAS) based regulatory network. All ECQ traits showed a large phenotypic variation and significant phenotypic correlations among the traits. TWAS analysis identified a total of 285 transcripts significantly associated with six ECQ traits. Genome-wide mapping of ECQ-associated transcripts revealed 66,905 quantitative expression traits (eQTLs), including 21,747 local eQTLs, and 45,158 trans-eQTLs, regulating the expression of 43 genes. The starch synthesis-related genes (SSRGs), starch synthase IV-1 (SSIV-1), starch branching enzyme 1 (SBE1), granule-bound starch synthase 2 (GBSS2), and ADP-glucose pyrophosphorylase small subunit 2a (OsAGPS2a) were found to have eQTLs regulating the expression of ECQ associated transcripts. Further, in co-expression analysis, 130 genes produced at least one network with 22 master regulators. In addition, we developed CRISPR/Cas9-edited glbl mutant lines that confirmed the role of alpha-globulin (glbl) in starch synthesis to validate the co-expression analysis. This study provided novel insights into the genetic regulation of ECQ traits, and transcripts associated with these traits were discovered that could be used in further rice breeding.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.55 no.3
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• pp.259-267
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• 2010

This study was performed at Rice and Winter Cereal Crops Department of NICS during 2007 and 2008 to investigate the characteristics of rice leaf emergence and to obtain basic data which can be used for rice growth simulation model by which we can forecast rice growth stage and heading date accurately under different cultivars, transplanting date, and climatic conditions. To confirm leaf emergence rate according to rice maturing ecotype, we surveyed the leaf emergence rate and heading date of Unkwangbyeo, Hwayoungbyeo and Nampyeongbyeo which are early maturing, medium maturing and medium-late maturing cultivars, respectively, according to seedling raising duration and transplanting time. When seedling duration was 15 days, the growth duration between transplanting time and completion of flag leaf emergence on main culm were 51.5~78.3 days in Unkwangbyeo, 55.3~87.9 days in Hwayoungbyeo and 58.4~98.4 days in Nampyeongbyeo, respectively. When seedling duration was 30 days, they were 50.1~75.5 days in Unkwangbyeo, 52.4~84.7 days in Hwayoungbyeo and 56.4~93.8 days in Nampyeongbyeo, respectively. As transplanting time delayed, the emerged leaf number after transplanting decreased in all rice cultivars. The cumulative temperature between transplanting time to completion of flag leaf elongation on main culm were $1,281^{\circ}C{\sim}1,650^{\circ}C$ in Unkwangbyeo, $1,344^{\circ}C{\sim}1,891^{\circ}C$ in Hwayoungbyeo and $1,454^{\circ}C{\sim}2,173^{\circ}C$ in Nampyeongbyeo, respectively. Leaf emergence rate on main culm were precisely represented by equation, y = $y_0$ + a / [1 + exp( - (x - $x_0$) / b)]^c, when we used daily mean temperature as variable.