• 한국임상수의학회지
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• 제28권1호
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• pp.57-62
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• 2011

The shortage of transplantable kidneys has many efforts to regenerate bioartificial kidneys using transgenic animals and diverse kinds of scaffolds which are important tools for cell seeding. However, there are many limitations for clinical applications so far. Recently, decellularized bioscaffolds using animal organs come into spotlight because of its many superior advantages. In current study, we produced decellularized kidney bioscaffolds of pig which is an attractive animal as a clinical model for human. We decellularized pig kidneys with 1% SDS detergent solution using peristaltic pump systems for 12h. After decellularization process, the kidney bioscaffolds preserved intact 3D morphology including glomerular structure and almost DNA from pig was entirely removed. In addition, this process could preserve micro vascular network which is necessary for cell survival. Although, additional studies for recellularization and transplantation should be required, the decellular vascularized kidney bioscaffolds might have many potentials for kidney regeneration.

• 대한수의학회지
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• 제60권4호
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• pp.229-232
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• 2020

A 15-year-old, spayed, female American shorthair cat presented with recurrent corneal sequestrum in the right eye. The cat had undergone superficial keratectomy twice for corneal sequestrum treatment 5 and 11 months previously. Two layers of porcine small intestinal submucosa (SIS; Vetrix BioSIS®) were applied to the surgical corneal defect after keratectomy. Thereafter, clinical signs, such as lacrimation, blepharospasm, and corneal ulcer, disappeared 50 days postoperatively. Moreover, the application of SIS with keratectomy prevented recurrence until 651 days after surgery. SIS could be applied as an additional bioscaffold for surgical repair of corneal sequestrum recurring after superficial keratectomy alone in cats.