Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Decellularized Bioscaffold of Pig Organs: A Tool for Patient-specific Organogenesis Using Induced Pluripotent Stem Cells

탈세포화 기법을 이용한 돼지 바이오 스캐폴드: 환자 맞춤형 장기재생을 위한 역분화 줄기세포 동물모델

  • 박경미 (강원대학교 줄기세포 연구소) ;
  • 곽호현 (강원대학교 줄기세포 연구소) ;
  • 남현숙 (강원대학교 줄기세포 연구소) ;
  • 박인철 (강원대학교 줄기세포 연구소) ;
  • 전용환 (강원대학교 줄기세포 연구소) ;
  • 박성민 (강원대학교 줄기세포 연구소) ;
  • 이승태 (강원대학교 줄기세포 연구소) ;
  • 우제석 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 우흥명 (강원대학교 줄기세포 연구소)
  • Accepted : 2011.02.16
  • Published : 2011.02.28
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Abstract

The shortage of transplantable kidneys has many efforts to regenerate bioartificial kidneys using transgenic animals and diverse kinds of scaffolds which are important tools for cell seeding. However, there are many limitations for clinical applications so far. Recently, decellularized bioscaffolds using animal organs come into spotlight because of its many superior advantages. In current study, we produced decellularized kidney bioscaffolds of pig which is an attractive animal as a clinical model for human. We decellularized pig kidneys with 1% SDS detergent solution using peristaltic pump systems for 12h. After decellularization process, the kidney bioscaffolds preserved intact 3D morphology including glomerular structure and almost DNA from pig was entirely removed. In addition, this process could preserve micro vascular network which is necessary for cell survival. Although, additional studies for recellularization and transplantation should be required, the decellular vascularized kidney bioscaffolds might have many potentials for kidney regeneration.

Keywords

References

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