• Journal of Applied Biological Chemistry
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• 제43권2호
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• pp.109-111
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• 2000

Effect of the two-stage operation and cell concentration on indirubin production was investigated using bioreactor culture of Polygonum tinctorium. Two-stage culture was operated successfully for 110 days without any adverse effects on continuous indirubin production. Maximum indirubin concentration was found to be at 80 mg/bioreactor. Initial cell concentration significantly affected indirubin production. The indirubin production at 29.2% PCV was improved by 845%, compared to that at 5% PCV. For high-density bioreactor culture of P. tinctorium, a maximum production rate of 10.2 mg indirubin/L day was obtained. Indirubin recovery for bioreactor operation was also examined using XAD-2, XAD-4, XAD-7, and solid silicon. XAD-4 was 1.6-fold more effective than that for solid silicon in indirubin recovery.

• KSBB Journal
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• 제27권1호
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• pp.28-32
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• 2012

In this study, the treatment ability of the wastewater containing perchlorate by non-salt tolerant perchlorate reducing bacterial consortium (N-PRBC) was evaluated in a continuous stirred tank bioreactor (CSTR). To obtain the optimal operating condition the bioreactor was operated with the different wastewater empty bed retention time (EBRT). The treatment performance in the bioreactor could be maintained at 100 $mg-ClO_4{^-}L^{-1}$ up to a EBRT of 3 h, and the removal capacity in the CSTR was about 3.3 times higher than that in a batch operation. With a decrease from 9 h to 2 h in a EBRT, the volumetric perchlorate reduction rate was increased from 11.1 $mg-ClO_4{^-}L^{-1}h^{-1}$ to 50.0 $mg-ClO_4{^-}L^{-1}h^{-1}$, and the specific perchlorate reduction rates were increased from 3.01 $mg-ClO_4{^-}g-DCW^{-1}h^{-1}$. In conclusion, the treatment capacities in a CSTR were much better than those obtained in a batch operation.

• 상하수도학회지
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• 제23권2호
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• pp.175-180
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• 2009

This study was conducted to improve biological degradation efficiency of toluene as a model volatile organic compound (VOC) using yeast Candida tropicalis and to suggest an effective method for bioreactor operation. The yeast strain was immobilized with polyethylene glycol (PEG), alginate, and powdered activated carbon (PAC). The yeast-immobilized polymer media were used as fluidized materials in an airlift bioreactor. Polymer media without PAC were also made and operated in another airlift bioreactor. The two bioreactors showed toluene removal efficiencies ranging 80-96% at loading rates of $10-35 g/m^3-hr$, and the bioreactor containing the polymer media with PAC achieved higher removal efficiency. Protein contents in the liquid phase showed that the bioreactor using the yeast-immobilized polymer media with PAC had a higher rate of microbial growth initially than that without PAC. In addition, the microbial growth rate inside of the polymer media with PAC was five times higher than that without PAC. Consequently, the polymer media containing the yeast strain and PAC could enhance removal efficiencies for VOCs, and the immobilization method improve microbial activity and stability for a long-term operation of biological systems.

• KSBB Journal
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• 제13권2호
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• pp.141-146
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• 1998

To remove the low concentration of methane biologically, a three phase fluidized bed bioreactor immobilized with Methylosinus trichosporium OB3b was used. Optimum pH, temperature and bed height for the operation were pH7.0, 30$^\circ C$ and 150cm, respectively. For the inlet methane concentration of 100-400ppm and flow rate of 2-4L/min, the removal efficiencies of the bioreactor using the activated carbon as a carrier were the range of 54-71%, whereas those using the biosand were the range of 45-56%. It was found that activated carbon was more efficient than the biosand for the removal of methane. When aeration tank was equipped with the bioreactor, the removal efficiency increased to 6-13% and maximum removal rate obtained in the experiment was 1184mg.CH$_4$/min.

• 상하수도학회지
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• 제25권4호
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• pp.565-572
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• 2011

The effects of chemical pretreatments on the excess sludge production in the membrane-coupled bioreactor were investigated. In addition, their effects on membrane fouling were also evaluated. Two membrane bioreactors were operated. In one reactor, a part of the mixed liquor was t reated with NaOH and ozone gas consecutively and was returned to the reactor. T he f lowrate of the chemical pretreatment stream was 1.5% of the influent flowrate. During the 200days of operation, the MLSS level in the bioreactor with mixed liquor pretreatment was maintained relatively constant at the range of 8,000 ~ 10,000$mg/{\ell}$ while it increased steadily up to 26,000 $mg/{\ell}$ in the absence of the pretreatment. Each reactor was equipped with two laboratory membrane modules where the flux for each module was 20, and 30 ${\ell}/m^2{\cdot}h$, respectively. With pretreatment, almost constant transmembrane pressure(TMP) was observed throughout the operation at the flux of 20 ${\ell}/m^2{\cdot}h$. Without pretreatment the membrane module at the same flux could also be operated at relatively stable condition. However, as the MLSS increases up to 25,000 $mg/{\ell}$, a fast TMP increase was observed. In conclusion, a complete control of excess sludge production in the membrane-coupled bioreactor was possible without significant deterioration of the treated water quality. In addition, it was shown that stable operation in terms of TMP is possible with sludge pretreatment and recirculation.

• 한국식품영양학회지
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• 제6권4호
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• pp.268-275
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• 1993

The whole cells of Actinoplanes missouriensis KCTC 1780 which produce glucose isomerase was immobillized by flocculation method for the effective production of high fructose syrup using packed-bed bioreactor system. Among the flocculation methods used In this study, the glucose Isomerase activity of flocculated cells using 5% polyethylenimmine and 0.2% glutaraldehyde was the highest as 46.3 unit, and the flocculant was 10.3g(wet weight) per 100m1 of broth, and the residual activity was 92.5%. In the batch operation of glucose isomerization using the flocculated cells, the optimum pH, temperature and isomerization ratio were 7.0, 75$^{\circ}C$ and 31%, respectively. The optimum concentration of Mg2+ which was activator on the glucose isomerization of flocculated cells was 0.1M, and glucose isomerase activity was increased by about 40% compared to none of Mg2+. In the packed-bed bioreactor system with 1.2 hour of residence time at 7$0^{\circ}C$, the reaction stability maintained until 96 hour without toss of activity, and the equilibrium was kept up to 120 hours of the operation.

• Membrane and Water Treatment
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• 제5권1호
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• pp.1-14
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• 2014

A pilot-scale hybrid membrane bioreactor (HMBR) for real municipal wastewater treatment was developed by adding biofilm carriers into a conventional membrane bioreactor, distribution and dynamic changes of the extracellular polymeric substances (EPS) and their roles in membrane fouling were investigated. The results showed that the concentrations of loosely bond EPS (LB-EPS) and tightly bond EPS (TB-EPS) in activated sludge, carrier biofilm and sludge cake layer have been increased significantly with the running time of HMBR, during operation of the HMBR, EPS demonstrated positive correlations with membrane fouling. Compared to TB-EPS, LB-EPS showed more significant correlations with sludge physical properties and specific resistance to filtration (SRF) in HMBR, and thus demonstrated that LP-EPS have a stronger potential of fouling than TB-EPS. It was also found that a lower organic loading in HMBR could result a significant increase in EPS concentration, which would in turn influence membrane fouling in HMBR. This critical investigation would contribute towards a better understanding of the behavior, composition and fouling potential of EPS in HMBR operation.

• 상하수도학회지
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• 제17권4호
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• pp.559-566
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• 2003

From this research, the performances of a sludge reduction in the sewage sludge aerobic digestion was experimented by using a sludge pretreatment and membrane bioreactor. The submerged plate membrane was used as the solid-liquid separation membrane. After drawing small amounts of sludge in a bioreactor and then doing the alkaline treatment and ozone treatment, the sludge was sent to back to the reactor. The HRT in the reactor was set as 5 days and the operation in the reactor was carried out at the DO of 1mg/L on average. After 100 days of operation in the reactor, it was shown that the reduction efficiency of total solids was more than 83%. Most of volatile solids were removed through mineralization, and the considerable portion of the non-volatile solids was dissolved and then flowed out with the effluent. Only about 16.3% of total solids in the sludge was accmulated in the reactor even without the loss of volatile fraction. Also, by deriving nitrification and denitrification in one reactor simultaneously, more than 90% of nitrogen removal effect was realized and the experiment was run smoothly without fouling of membrane, even in the high concentration of MLSS. Based on this experiment, sludge can be reduced considerably at a low HRT by these two newly suggested approach.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
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• pp.73-76
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• 2000

A novel, continuous bioreactor system combining a bubble column (absorption section) and a two-phase bioreactor (degradation section) has been designed to treat a gas stream containing benzene. The bubble column contained hexadecane as an absorbent for benzene, and was systemically chosen considering physical, biological, environmental, operational and economic factors. This solvent has infinite solubility for benzene and very low volatility. After absorbing benzene in the bubble column, the hexadecane served as the organic phase of the two-phase partitioning bioreactor, transferring benzene into the aqueous phase where it was degraded by Alcaligenes xylosoxidans Y234. The hexadecane was then continuously recirculated back to the absorber section for the removal of additional benzene. All mass transfer and biodegradation characteristics in this system were investigated prior to operation of the integrated unit, and these included: the mass transfer rate of benzene in the absorption column, the mass transfer rate of benzene from the organic phase into the aqueous phase in the two-phase bioreactor, the stripping rate of benzene out of the two-phase bioreactor, etc. All of these parameters were incorporated into model equations, which were used to investigate the effects of operating conditions on the performance of the system. Several experiments were conducted to show the feasibility of this system. This process is believed to be very practical for the treatment of high concentrations of gaseous pollutants.

• 한국물환경학회지
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• 제21권4호
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• pp.353-359
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• 2005

This study was conducted to evaluate the operation characteristics with aeration time in intermittent aeration membrane bioreactor. The BOD removal efficiency rate of this process was over than 97% regardless of aeration on/off time. To get over than 82% of nitrogen removal efficiency rate, aeration off time needs more than 70 minutes in reactor. Specific denitrfication rate was 2.68 mg $NO_3-N/gMv/hr$ in 40/80 min aeration on/off time, was 2.6 times more than 60/60 min, and 1.4 times more than 50/70 min in 6,300 mg/L of MLSS concentration. Specific nitrification rate was 1.96 mg $NH_4-N/gMv/hr$ in 50/70 min, was 1.4 times more than 40/80 min, but it was effectded little upon nitrification. Microbial activity was effected little according to aeration on/off time, oxygen demend was reduced according to aeration off time increased and microbial concentration increased. The longer aeration off time become, the higher Extraceller Pollymeric Substance (EPS), 50/70 min and 40/80 min in aeration on/off time was increased 1.6 times and 2.7 times, respectively more than 60/60 min because of increase of operation pressure.