• Proceedings of the Korean Biophysical Society Conference
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• 2002.06b
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• pp.16-17
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• 2002

The major histocompatibility complex (MHC) is an important susceptibility locus for many human autoimmune diseases. The structural and functional properties of HLA-DR molecules that are associated with susceptibility to several autoimmune diseases, such as rheumatoid arthritis and multiple sclerosis, have been defined.(omitted)

• Journal of Photoscience
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• v.9 no.2
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• pp.102-105
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• 2002

Phoborhodopsin (pR or sensory rhodopsin II, sRII; the absorption maximum of ∼ 500 nm) is a retinoid protein and works as a photoreceptor of the negative phototaxis of Halobacterium salinarum. pharaonis phoborhodopsin (ppR or pharaonis sensory rhodopsin II, psRII) is a corresponding protein of Natronobacterium pharaonis. These sensory proteins form a complex with a cognate transducer protein in the membrane, and this complex transmits the light-signal to the cytoplasm to evoke avoidance reaction from blue-green light. Recently, the functional expression in Escherichia coli membrane of ppR was achieved, which can afford a large amount of the protein and enables mutant studies to clarify the role of various amino acid residues. A truncated transducer which can bind to ppR is also expressed in Escherichia. coli membrane. In this article, we will review properties of ppR mainly using observations of our laboratory; which contains photochemistry (photocycle), light-driven proton uptake, release and transport, F -helix titling during photocycle and association of the transducer.

• Proceedings of the Korean Biophysical Society Conference
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• 1998.06a
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• pp.29-29
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• 1998

Previously, we reported two types of vanadate-sensitive ATPases in the micro somes of tracheal epithelial cells, a high-affinity one and a low-affinity one. The low affinity vanadate-sensitive (LAVS) ATPase was sensitive to thapsigargin and cyclopiazonic acid, specific antagonists of ER-type Ca$\^$2＋/-ATPase, and mediated microsomal $\^$45/Ca$\^$2+/ uptake, implying that the LAVS-ATPase is an ER/SR-type Ca$\^$2+/-ATPase.(omitted)

• Proceedings of the Korean Biophysical Society Conference
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• 1998.06a
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• pp.33-33
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• 1998

Oxidation and reduction of amino acid residues in proteins affect their functional properties. Especially, redox modulation of ion channel activities has been reported in number of ion channel proteins. In this study, we investigated the effects of tertiary-butyl hydrogen peroxide (tBHP) on the large-conductance Ca$\^$2＋/ -activated K$\^$+/(Maxi-K) channel of rat brain using lipid bilayer reconstitution technique.(omitted)

• Proceedings of the Korean Biophysical Society Conference
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• 1996.07a
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• pp.25-25
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• 1996

A mature mutant ribose binding protein (RBP) of Escherichia coli was obtained by site-directed mutagenesis, replacing Thr-3 in the N-domain of wild-type mature RBP (WT -mRBP) with a Trp residue (N- Trp-mRBP). The equilibrium unfolding properties and the refolding kinetics of this protein were monitored by fluorescence and circular dichroism (CD). (omitted)

• Proceedings of the Korean Biophysical Society Conference
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• 1996.07a
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• pp.27-27
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• 1996

SecA protein which has a pivotal role in the preprotein cranslocation across the inner membrane of Escherichia coli is a water-soluble protein with an unusual property of penetrating the membrane readily. An interesting and important question is what structural characteristics of SecA enables its ready penetration of lipid bilayer. The conformational properties of SecA in solution at 3$0^{\circ}C$, pH 7.5 were observed by hydrogen-tritium (HT) exchange, and denaturant-induced denaturation/renaturation and thermal unfolding. (omitted)

• Proceedings of the Korean Biophysical Society Conference
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• 2002.06b
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• pp.9-11
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• 2002

Plasma membrane Na$^{＋}$-K$^{＋}$ ATPase (pump) is an essential component to maintain asymmetrical ion distribution across cell membrane. The Na$^{＋}$-K$^{＋}$ ATPase was discovered by Jens C. Skou in 1957 and since then physiological and biochemical properties of the enzyme have been extensively studied. Jens C. Skou was awarded the 1997 Nobel Prize in chemistry for his discovery of the Na $^{＋}$ - $K^{＋}$ ATPase.(omitted)

• Proceedings of the Korean Biophysical Society Conference
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• 1999.06a
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• pp.60-60
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• 1999

The subfornical organ (SFO) represents neuroglial circumventricular organ structures bordering the anterior third cerebral ventricle. Owing to the absence of the blood-brain barrier, the cellular elements of subfornical organ can be reached by circulating messenger molecules transferring afferent information and provide a good coding model of chemical information processing in the body.(omitted)

• Proceedings of the Korean Biophysical Society Conference
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• 1997.07a
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• pp.18-18
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• 1997

Chronic treatment with cyclosporin A (CsA) were shown to induce reversible alterations of contractile properties in rat heart. To define the molecular mechanisms underlying the physiological alterations, the $Ca^{2＋}$ release channel (CRC) and $Ca^{2＋}$-ATPase in rat sarcoplasmic reticulum (SR) were examined.(omitted)

• Proceedings of the Korean Biophysical Society Conference
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• 1997.07a
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• pp.29-29
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• 1997

Cyclosporin A (CsA) is widely used to suppress rejection in rcipients of solid organ or bone marrow transplants. A variety .of toxic side effects of this agent such as cardiotoxicity have been reported. However the underlying molecular mechanisms for the cardiotoxicity are not well resolved.(omitted)