• Proceedings of the Korean Biophysical Society Conference
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• 2002.06b
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• pp.50-50
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• 2002

The heterologous expression of sodN gene from Streptomyces seoulensis in Streptomyces lividans together with the gel filtration and sedimentation equilibrim data indicated that the quaternary structure of NiSOD is homohexamer, which is novel among SODs, not the previously reported homotetramer. The EPR spectrum of $^{61}$ Ni (I = 3/2) substituted NiSOD showed a clear resolved hyperfine structure at g=2.016, unambiguously identifying that the EPR signal from NiSOD is due to Ni.(omitted)

• Proceedings of the Korean Biophysical Society Conference
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• 2003.06a
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• pp.36-36
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• 2003

Goldfish retina has been well studied to a great extent. In spite of that, electrical characteristics of dissociated horizontal cells(HCs) have not been identified in detail. Thus the cone-and the rod- HCs dissociated from goldfish retina were investigated electrophysiologically using whole-cell patch-clamping recording. To explore the basic electrical property, We examined voltage-dependent channels in all types of HCs. For the futher understanding of GABAergic pathway, the localization and distribution of GABA receptors was examined in cone- HCs including HC axon terminals(ATs).

• Journal of Biomedical Engineering Research
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• v.32 no.3
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• pp.270-277
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• 2011

Laser cartilage reshaping(LCR) is a promising method for reshaping cartilage by using laser irradiation to maintain permanently modifies its shape. However this method has not been fully understood due to the limited scientific researches. The purpose of this study is to analyze optical and thermal characteristics of cartilage during laser irradiation. After analyzing Monte Carlo simulation for the comparison of laser fluence distributions with different laser wavelengths the characterization of the spectral changes during Nd:YAG laser(${\lambda}$ = 1444 nm) irradiation was investigated in the ranges of 900-1700 nm with double integrating spheres. The surface temperature distribution changes during laser irradiation were investigated with an infrared camera. The quantitative measurements of optical and thermal characteristics in cartilage after laser irradiation were correlated with the transition of water flux(from bound to free water) and this study may be useful for better understanding of biophysical transformation phenomena in cartilage after laser heating.

• Korean Journal of Remote Sensing
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• v.22 no.6
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• pp.533-542
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• 2006

Lidar point clouds provide three dimensional information of terrain surface and have a great advantage to generate precise digital elevation model (DEM), particularly over forested area where some laser signals are transmitted to vegetation canopy and reflected from the bare ground. This study initially investigates the characteristics of lidar-derived height information as related to vertical structure of forest stands. Then, we propose a new filtering method to separate ground points from Lidar point clouds, which is a prerequisite process both to generate DEM surface and to extract biophysical information of forest stands. Laser points clouds over the forest stands in central Korea show that the vertical distribution of laser points greatly varies by the stand characteristics. Based on the characteristics, the proposed filtering method processes first and last returns simultaneously without setting any threshold value. The ground points separated by the proposed method are used to generate digital elevation model, furthermore, the result provides the possibilities to extract other biophysical characteristics of forest.

• Proceedings of the Korean Biophysical Society Conference
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• 2003.06a
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• pp.43-43
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• 2003

Large-conductance $Ca^{2+}$-actived $K^{+}$ channels ($BK_{Ca}$ channels) play a key role in setting the pace of contractile activity in muscle and are involved in the regulation of neurotransmitter release in neuron. $BK_{Ca}$ channels are activated by depolarizing membrane potential and the elevated level of intracellular calcium. Using yeast-two hybrid assay, we have identified a novel protein interacting with the cytosolic carboxyl terminus of rSlo, the brain isoform of rat large-conductance $Ca^{2+}$-activated $K^{+}$ channel $\alpha$-subunit. The novel gene encodes 51 kDa protein and is named as SIRK(rSlo-interacting RGS-like protein). SIRK is expressed in various tissues and localized in the cytosolic and the membrane fraction. Biochemical and immunological studies indicated that SIRK physically interacted with the cytosolic region of rSlo. To investigate whether SIRK can modulate the activity of rSlo, GFP-fused SIRK and rSlo were transiently transfected into COS-7 cells and the effects of SIRK was studied using electrophysiological means. We concluded that the overexpression of SIRK alters the surface expression of rSlo channel with only a limited effect on the biophysical characteristics of the channel.the channel.

• Environmental and Resource Economics Review
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• v.10 no.1
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• pp.95-126
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• 2001

Functional values for wastewater treatment and atmospheric regulation in coastal wetland and rice paddy ecosystems are quantified, and an illustration is given on how to integrate biophysical parameters into a valuation framework. This is one of most controversial issues in economic analysis for wetland preservation versus wetland conversion to agricultural use. This paper includes theoretical considerations for estimating functional values of environmental ecosystems, and the integration of biophysical data and replacement cost method employed. Specific physical and geographical characteristics and data on ecosystem functions and services in coastal wetlands and rice paddies are addressed for evaluating their values in economic terms. In particular this paper indicates double counting problems and overestimation in the previous studies, and demonstrates how to avoid them and to maintain the consistency of valuation process involving a least-cost method, thus enables an accurate integration of the coastal wetland ecology and wetland economics. As a result which is far away from the previous studies, the total economic present value of wastewater assimilation by coastal wetland is estimated at 7,484,640 won/ha, and the net present value of positive effect for atmospheric regulation, negative effects for air pollution and water pollution by rice paddy is estimated at -37,934 won/ha, assuming that resources are infinitely long-lived and the annual value and the rate of discount (10%) is constant every year. In conclusion, for further reliability and validity of functional values for natural resources it is very noteworthy that a general equilibrium framework that could directly incorporate the interdependence between ecosystem functions and services would be preferred to the partial equilibrium framework.

• Proceedings of the Korean Biophysical Society Conference
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• 2003.06a
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• pp.25-25
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• 2003

Atrial myocytes have two functionally separate $Ca^{2+}$ release sites: those in peripheral sarcoplasmic reticulum (SR) adjacent to the $Ca^{2+}$ channels of surface membrane and those in central SR not associated with $Ca^{2+}$ channels. Study on the spatio-temporal properties of focal $Ca^{2+}$ releases (“sparks”) occurring spontaneously in central and peripheral sites of voltage-clamped rat atrial myocytes, using rapid two-dimensional (2-D) confocal $Ca^{2+}$ imaging revealed that peripheral and central sparks were similar in size and release time (~300,000 $Ca^{2+}$ ions for=12 ms), but significantly larger and longer than ventricular sparks. Both sites were resistant to Cd$^{2+}$ and inhibited by ryanodine. Peripheral sparks were brighter and flattened against surface membrane, had ~5-fold higher frequency, ~2 times faster diffusion coefficient, and dissipated abruptly. Central sparks, in contrast, occurred less frequently, were elongated along the cellular longitudinal axis, and dissipated slowly. Compound sparks (composed of 2-5 unitary focal releases) aligned longitudinally, occurred more frequently at the center.at the center.

• Journal of Radiation Protection and Research
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• v.30 no.1
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• pp.27-30
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• 2005

New possibilities are discussed for cholesteric liquid crystals (CLC) as sensor materials for detection of ionizing radiation, biologically active UV radiation, and the presence of hazardous vapors in atmosphere. A distinguishing property of CLC-based detectors is their 'bioequivalence', i.e., mechanisms of their response to external factors essentially imitate the corresponding mechanisms of biological tissues. Such detectors can ensure sufficiently high sensitivity to make feasible their use as alarm indicators or in biophysical studies. Specific examples ate given of sensor compositions and their response characteristics.

• Proceedings of the Korean Institute of Intelligent Systems Conference
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• 1993.06a
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• pp.1013-1016
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• 1993

This paper describes a new type of neuron model, the inputs of which are interfered with one another. It has a high mapping ability with only single unit. The learning speed is considerably improved compared with the conventional linear type neural networks. The proposed neuron model was successfully applied to the prediction problem of chaotic time series signal.

• Korean Journal of Veterinary Research
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• v.32 no.1
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• pp.77-82
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• 1992

A noncytopathic bovine viral diarrhea virus(NC BVDV) strain isolated and purified from persistently infected primary bovine fetal lung(BFL) cells was studied by biophysical methods. The buoyant density of particles of the NC BVDV strain was shown to be between 1,090 and $1,114g/cm^3$ and the maximum virus infectivity occured at $1,098g/cm^3$. Immunoelectron microscopic examination by using the partially purified virus revealed regular spherical particles 30~80nm in diameter. Differences in the genomic size of cytopathic and noncytopathic BVDV from infected cells were not found. A comparison of viral proteins of a noncytopathic and cytopathic strain(NADL) by immunoprecipitation using monoclonal antibody indicated that NC BVDV, compared to cytopathic NADL, was cell associated.