• Title/Summary/Keyword: biomimetic method

검색결과 68건 처리시간 0.026초

Synthesis and physicochemical characterization of NixZnx-Fe2O4/MWCNT nanostructures as enzyme mimetics with peroxidase-like catalytic activity

  • Salarizadeh, Navvabeh;Sadri, Minoo;Hosseini, Hassan;Sajedi, Reza. H.
    • Carbon letters
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    • 제24권
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    • pp.103-110
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    • 2017
  • Carbon-based magnetic nanostructures in several instances have resulted in improved physicochemical and catalytic properties when compared to multi-wall carbon nanotubes (MWCNTs) and magnetic nanoparticles. In this study, magnetic MWCNTs with a structure of $Ni_xZn_xFe_2O_4/MWCNT$ as peroxidase mimics were fabricated by the one-pot hydrothermal method. The structure, composition and morphology of the nanocomposites were characterized with X-ray diffraction (XRD), Fourier transform infrared spectroscopy and transmission electron microscopy. The magnetic properties were investigated with a vibrating sample magnetometer. The peroxidase-like catalytic activity of the nanocomposites was investigated by colorimetric and electrochemical tests with 3,3',5,5'-tetramethylbenzidine (TMB) and $H_2O_2$ as the substrates. The results show that the synthesis of the nanocomposites was successfully performed. XRD analysis confirmed the crystalline structures of the $Ni_xZn_xFe_2O_4/MWCNT$ nanohybrids and MWCNTs. The main peaks of the $Ni_xZn_xFe_2O_4/MWCNT$s crystals were presented. The $Ni_{0.25}Zn_{0.25}Fe_2O_4/MWCNT$ and $Ni_{0.5}Zn_{0.5}Fe_2O_4/MWCNT$ nanocatalysts showed nearly similar physicochemical properties, but the $Ni_{0.5}Zn_{0.5}Fe_2O_4/MWCNT$ nanocatalyst was more appropriate than the $Ni_{0.25}Zn_{0.25}Fe_2O_4/MWCNT$ nanocatalyst in terms of the magnetic properties and catalytic activity. The optimum peroxidase-like activity of the nanocatalysts was obtained at pH 3.0. The $Ni_{0.5}Zn_{0.5}Fe_2O_4/MWCNT$ nanocatalyst exhibited a good peroxidase-like activity. These magnetic nanocatalysts can be suitable candidates for future enzyme-based applications such as the detection of glucose and $H_2O_2$.

An Experimental Study of Comfortable Pitch and Loudness with Target Matching: Effects on Electroglottographic and Acoustic Measures

  • 최성희
    • 말소리와 음성과학
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    • 제4권4호
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    • pp.139-146
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    • 2012
  • This study was designed to examine comfort levels of pitch and loudness with target matching and their effects on electroglottographic (EGG) and acoustic measures. Twelve speakers, six males and six females, were instructed to produce /a/ sustained vowel for three seconds at a comfortable pitch and loudness level without any instruction and with a target matching procedure of either a certain f0 or SPL separately with visual and auditory feedback. The range of pitch for females and males were presented by progressing up and down randomly at intervals of 5Hz from 150 Hz to 310 Hz (total 33 frequency targets) and from 85 Hz to 190 Hz (total 22 frequency targets), respectively. The loudness levels were 65, 75, 85, 95 dB (total of four intensity targets) for both males and females. Subjective estimations of comfortable levels were obtained using a 10-point equal-appearing interval rating scale following each phonation. The results showed that males and females demonstrated similar trends in loudness levels with greatest comfort at 75 dB, whereas pitch comfort ratings showed a greater variability with females having a wider range with target matching. In the comfort levels of individuals, most male and female speakers rated higher comfort at soft, rather than loud phonations. On the other hand, most male speakers perceived highest comfort levels below the comfort pitch levels they phonated under natural conditions. Higher frequency ranges, however, were perceived to be more comfortable than those of natural condition in most female speakers, although the comfortable pitch levels in spontaneous phonations were within the comfort level ranges determined by targeted phonations. When comparing acoustic (%jitter, %shimmer, SNR) and EGG measures (CQ%) between spontaneous comfortable phonations and targeted phonations produced by the same subject at similar f0 and intensity, no significant differences were observed (p>0.05). Thus, target matching procedures may be considered a compatible and alternative method to reduce the variability of comfortable pitch and loudness levels by eliciting consistent comfortable phonations.

형상기억합금 코일 구동기와 복합재를 이용한 소형 도약 로봇 설계 및 제작 (Miniature Jumping Robot Using SMA Coil Actuators and Composite Materials)

  • 정순필;고제성;정광필;조규진
    • 로봇학회논문지
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    • 제8권2호
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    • pp.136-142
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    • 2013
  • In nature, many small insects are using jumping as a survival strategy. Among them, fleas jump in a unique method. They use an elastomer, 'Resilin', an extensor muscle and a trigger muscle. By contracting the extensor muscle, the elastic energy, that makes a flea to jump, is stored in the resilin. After storing energy, the trigger muscle begins contracting and pulling the extensor muscle. When the extensor muscle crosses the rotational joint, direction of torque generated from the extensor muscle reverses, 'torque reversal mechanism'. Simultaneously, the elastic energy stored in the resilin releases rapidly and is converted into the kinetic energy. It makes a flea to jump 150 times its body length. In this paper, miniaturized jumping robot using flea-inspired catapult mechanism is presented. This mechanism is based on the 4-bar linkage and the reversal joint and is actuated by Shape Memory Alloy (SMA) coiled springs describing the flea's muscle. The robot prototype is fabricated by SCM process using glass fiber prepregs and a sheet of polyimide film. The prototype is 20mm link length, 34mm width and 2.0g weight and can jump 103cm.

다중 영상을 이용한 생체모방형 물체 접근 감지 센서 (Biomimetic approach object detection sensors using multiple imaging)

  • 최명훈;김민;정재훈;박원현;이동헌;변기식;김관형
    • 한국정보통신학회:학술대회논문집
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    • 한국정보통신학회 2016년도 춘계학술대회
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    • pp.91-93
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    • 2016
  • 2차원 영상으로부터 3차원 정보를 추출하는 과정은 매우 중요한 단계로서 하나의 카메라를 이용하는 단안시법과 두 개의 카메라를 이용하는 양안 시법이 있는 후자를 일반적으로"스테레오 비전"이라고 한다. 요즘 많이 CCTV나 여러 매체에서 사용되고 있는 자동 물체추적 시스템에서 인간의 두 눈을 모방한 스테레오 카메라를 이용하여 현장의 상황이나 작업 전개를 보다 명확하게 알 수 있어 회피/제어 기동 및 여러 작업의 효율을 극대화할 수 있다. 기존의 2D 영상에서의 물체 추적시스템은 거리를 인식할 수 없어 전이를 인식할 수 없었으나 스테레오 영상의 시차를 이용하고 객체를 표시하여 관측자가 보다 효과적으로 제어할 수 있을 것이다.

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감마선 조사법을 이용한 전기방사 나노/마이크로 섬유 지지체의 표면 기능화 (Functionalization of Electrospun Nano/Micro-fibrous Scaffolds Using Gamma-ray Irradiation)

  • 임종영;신영민;최종배;정진오;권희정;정성린;박종석;임윤묵
    • 방사선산업학회지
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    • 제7권1호
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    • pp.45-49
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    • 2013
  • In tissue engineering application, a fibrous structure of scaffolds has been issued as an alternative system to regulate cell survival and tissue regeneration, and electrospinning technique has been popularly used to generate fibrous meshes or sheets mimicking the structure of native extracellular matrix (ECM). However, recent strategy in the scaffold development is expanded to provide the structural property as well as a biological property of native ECM, a variety of surface modification techniques have been used to introduce biological property. In this study, we developed biomimetic poly(L-lactide-co-${\varepsilon}$-caprolactone) (PLCL) nano- and micro-fibrous scaffolds as a unique platform with structural and biological properties with native ECM using electrospinning method and gamma-ray irradiation. Surface morphology of the scaffolds was observed by scanning electron microscopy, and alteration of surface property was evaluated with toluidine blue O staining, water contact angle measurement and ATR-FTIR analysis.

4톤급 지게차 냉각홴 소음 저감에 관한 실험적 연구 (An Experimental Study on the Noise Reduction of Cooling Fans for Four-ton Forklift Machines)

  • 최대식;김석우;염태영;이승배
    • 드라이브 ㆍ 컨트롤
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    • 제18권1호
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    • pp.1-8
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    • 2021
  • This paper presents research on methods for the reduction of forklifts' noise level for the increased comfort and safety of its operator. A cooling fan with a high air volume flow rate installed in the forklift acts as an important design parameter which efficiently cools the heat exchanger system, helping to transfer internal heat from the engine room to the outdoors with both transmitted and diffracted opening noises. The cooling fan contributes significantly to both the forklift's emitted sound power and the operator room's noise level, thereby necessitating research on the forklift's reduction of acoustic power level and transmission. A noise analysis for various fan models with a biomimetic design based on eagle-wing geometry was conducted. In addition to the acoustic power generation, the aerodynamic performance of the cooling blade is also strongly influenced by the design of airfoil distribution, thereby requiring optimization. The cooling fans were fabricated and installed in the forklift in order to check the efficacy of the forklift engine's cooling, and the final version of the fan was measured for its ability to lower acoustic power level and cool the engine room. This study explains the aerodynamic and acoustic features of the designed fans with the use of BEM analysis and forklift test results.

생체모방 모세관 현상을 이용한 PDMS 구조체 기반 증산발전기 제조기술 연구 (Research on the Manufacturing Technology for a PDMS Structure-Based Transpiration Generator Using Biomimetic Capillary Phenomenon)

  • 이승환;윤정재;백소현;권용범;송요셉;김범성;좌용호;정다운
    • 한국분말재료학회지
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    • 제30권3호
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    • pp.268-275
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    • 2023
  • The demand for energy is steadily rising because of rapid population growth and improvements in living standards. Consequently, extensive research is being conducted worldwide to enhance the energy supply. Transpiration power generation technology utilizes the vast availability of water, which encompasses more than 70% of the Earth's surface, offering the unique advantage of minimal temporal and spatial constraints over other forms of power generation. Various principles are involved in water-based energy harvesting. In this study, we focused on explaining the generation of energy through the streaming potential within the generator component. The generator was fabricated using sugar cubes, PDMS, carbon black, CTAB, and DI water. In addition, a straightforward and rapid manufacturing method for the generator was proposed. The PDMS generator developed in this study exhibits high performance with a voltage of 29.6 mV and a current of 8.29 µA and can generate power for over 40h. This study contributes to the future development of generators that can achieve high performance and long-term power generation.

배양연골막이 피복된 고효능 인공연골의 생체내 효과 (The Effect of Cultured Perichondrial Cell Sheet Covered Highly Active Engineered Cartilage: in vivo Comparative Assessment)

  • 박세일;문영미;정재호;장광호;안면환
    • 한국임상수의학회지
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    • 제28권5호
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    • pp.486-496
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    • 2011
  • 조직공학적 인공연골재생에 대한 관심이 증가함에 따라 많은 연구들이 활발히 수행되고 있으나 임상적인 적용의 한계를 극복하기위한 고효능을 보유한 양질의 연골조직생산의 필요성이 증가되고 있다. 인공연골은 자연연골과는 달리 '연골막(perichondrium)'을 포함하고 있지 않기 때문에 장기간 생체 내에 삽입된 후에 서서히 흡수 또는 변형으로 임상적 활용에 한계가 있다고 있다. 이에 본 연구는 양질의 연골조직생산을 목적으로, 세포판 제작기법(cell sheet engineering technique) 을 기반으로 한 인체유래의 배양 연골막(cultured perichondrium)을 이용하여 만든 인공연골막 세포판(cultured perichondrial cell sheet)의 생체 내 특성을 비교 분석하고, 배양된 연골막을 피복하여 고효능화를 유도한 인공연골복합체의 생체내 재생효능 및 조직특성을 비교 평가하고자 하였다. 본 연구에서는 Athymic nude mouse의 피하이식모델(study 1, n = 12)을 이용하여 담체로 hydrogel을 이용한 배양연골막 복합체의 생체내 효능을 분석하였고, 중대형동물의 대량연골 결손시의 재생효능을 평가하기 위하여 개의 무릎연골에 $1{\times}2cm$의 대량연골 결손모델(study 2, n = 12)을 통하여 인공배양세포판을 이식하였다. 이식12주 후 이식편을 회수하여 생화학, 분자생물학 및 면역조직학 분석을 시행한 결과, 배양연골막 복합체의 생체내 효능이 단독이식군에 비해 변형이나 과증식 없이 우수한 결과를 나타내었다. 본 연구의 결과로 토대로 배양연골막을 피복한 인공연골막의 관절내 효과를 규명하여 실제 임상적용을 조기화하는 기반을 제공하고 인공연골의 문제점이었던 변형과 흡수를 줄인 고효능 인공연골 제작기법을 제공하는데 유용할 것으로 기대된다.