• The Journal of the Society of Korean Medicine Diagnostics
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• v.10 no.2
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• pp.79-87
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• 2006

Objective : Biologic responses to color stimulation was observed and analyzed in relation with Korean medicine diagnostics. Methods : Twelve volunteers participated and completed the questionnaire on Hanyeol or Joseup patterns of Korean medicine diagnostics. Standard deviation of RR intervals (SDNN), an overall indicator of heart rate variability, was measured while wearing partially transparent acetate film glasses of various colors: (1) transparency or black, (2) red, green, or blue, or (3) cyan, or yellow. Results : Different responses to the color environmental stimulation were observed. Yeol pattern score was inversely related with the SDNN value under red color stimulation. Conclusion : This line of observations may serve as a basis of clinical application of color therapy, in particular, in relation with pattern diagnosis of Korean medicine, and may be applied in combination with existing electrodermal meridian measurement systems for better evaluation of Gi (life energy) phenomenon.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.40
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• pp.16.1-16.5
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• 2018

Background: Proliferative verrucous leukoplakia (PVL) is an oral potentially malignant disorder, characterized by multifocal expression, progressive clinical evolution, and a high rate of malignant transformation. Evidence-based information regarding optimal PVL management is lacking, due to the paucity of data. The present report describes a case of PVL associated with HPV-16 infection and epithelial dysplasia treated by diode laser surgery, and the outcome of disease clinical remission over a 2-year follow-up period. Case report: A 61-year-old Caucasian male with oral verrucous hyperkeratosis presented for diagnosis. The lesions were localized on the maxillary gingiva and palatal alveolar ridge. Multiple biopsy specimens have been taken by mapping the keratotic lesion area. Microscopic examination was compatible with a diagnosis of PVL with focal mild dysplasia, localized in the right maxillary gingiva. Polymerase chain reaction (PCR) was done for human papillomavirus (HPV) detection which revealed presence of HPV DNA, and the genotype revealed HPV 16 in the sample. The PVL in the right gingival area was treated on an outpatient basis by excision with a diode laser. This approach resulted in good clinical response and decreased morbidity over a 2-year follow-up period. Conclusions: This case illustrates the benefit of a conservative approach by diode laser treatment than wide surgical excision for management of the PVL lesions associated with mild dysplasia and HPV-16 infection.

• Biomedical Science Letters
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• v.17 no.4
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• pp.355-361
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• 2011

We evaluated the performance of the NOW $Streptococcus$ $pneumoniae$ urinary antigen test, standard culture and polymerase chain reaction for detecting $S.$ $pneumoniae$. The urinary antigen test of pneumonia patients represented sensitivity at 72% and specificity at 79%. The results of PCR were targeting for autolysin ($lyt$A), pneumolysin ($ply$), and spn9828. The $lyt$A sensitivity and specificity stood at 56% and 87% respectively while $ply$ sensitivity reported 83% and specificity was 47%, sensitivity and specificity of spn9828 stood at 83% and 73% respectively. The results of urinary antigen test and three genes were all statistically meaningful within $P$ <0.05. When the urinary antigen test of $S.$ $pneumoniae$ was positive, the three kinds of genes were also likely to be positive. According to the result of urinary antigen test, the results of PCR presented a meaningful difference ($P$ <0.05). Especially, the urinary antigen test of $S.$ $pneumoniae$ was likely to be positive ($P$ <0.05) when more than two genes were positive in PCR results.

• Journal of Biomedical Engineering Research
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• v.41 no.4
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• pp.165-171
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• 2020

In this study, it focuses on the development of radiographic devices for patellar imaging. On the previous X-ray examination of patella and patellofemoral joint, it analyzed the problems which could affect the results: unstable patients' pose, radiation exposure, and deterioration of image quality. The purpose of the research is to propose a future use of a developed device with an explanation of the process, function, and advantage of the device including the methods. The device is developed to focus on the diagnosis of the longitudinal and longitudinal fractures of the patella and patella/femoral joint, displacement such as dislocation, bone formation, stenosis of the patella/femorla joint, and cartilage wear. Due to the patient's anxious posture, it caused a shaking image, overlapping structures, and etcs, these factors challenge to diagnose accurately. In addition, the existing Settegast method and Hughston method, which are most frequently performed in the hospital field, are not suitable for the presence or absence of features or dislocation due to the heavy load on the patella of the patient. The developed device requires patients in a lying position and placing only their leg on the device, it increases the conveniences of the examination and decreases unnecessary radiation exposure of the patient except the patellar examination area. Moreover, one of the systems in the device fixes the detector, where the patients no longer need to hold the detector nor be in unstable posture, but describes the structure of the patella/femoral joint more clearly. Hoping the device will apply to more patients.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2013.10a
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• pp.513-516
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• 2013

In this paper, we present an information collecting system in medical information management domain. Our proposed system performs a systemized process, consisting of collection, transmission, and management, to develop intelligent medical information system and medical big data processing system. Our information collecting system consists of low-power biomedical sensors, biomedical information collecting devices, and storage systems. Currently, almost biomedical information of patients is collected manually by employees like nurses and medical doctors. Therefore, collected biometric data can be error-pronoun data. Since there is a lack to make big data of medical information, it is difficult to enhance the quality of medical services and researches. Accordingly, through our proposed system, we can overcome the problems like error-pronoun biometric data. In addition, we can extremely extend the area of collectable biometric data. Furthermore, using this system, we are able to make a real-time biomedical analysis system, like a real-time patient diagnosis system, and establish a strategy to against future medical markets changing rapidly.

• Biomedical Science Letters
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• v.22 no.3
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• pp.83-97
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• 2016

The measurement of viral load in HIV-1 infected patients is essential for the establishment of a therapeutic strategy. Several commercial assays have shown shortcomings in quantifying rare genotypes of HIV-1 such as minor groups of N and O. In this study, the HIV-1 RT-qPCR assay was developed. The primers and probe of HIV-1 were designed to target the pol gene and to increase the detection efficiency of various subtypes including group N and O. The HIV-1 quantitative RT-qPCR assay was assessed for its analytical performance and clinical evaluation. The LoD was determined to 33.9 IU/ml. The LoD of several subtypes including A, C, D, CRF_01AE, F, CRF_02AG, G and H, were determined to less than 40 IU/ml. The HIV-1 quantitative RT-qPCR assay was evaluated using the China National Reference Panel of HIV-1 RNA to determine the analytical performance. The results were all within the acceptable range. The clinical evaluation was performed at Hunan CDC in China. The clinical evaluation results were compared with those of the China domestic commercial kit. A significant correlation (fresh samples; $R^2=0.84$, P<0.001, frozen samples; $R^2=0.76$, P<0.001) between the two systems was observed for 64 fresh samples and 76 frozen samples with viral loads, and the Bland-Altman plot showed good agreement (98.4%, 96.1%, respectively). In conclusion, the HIV-1 quantitative RT-qPCR assay had comparable analytical performance with several commercial kits. The study provides basic data for the research of HIV-1 diagnosis and the development of P < HIV-1 molecular diagnostic assay.

• BMB Reports
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• v.53 no.6
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• pp.299-310
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• 2020

Chronic liver disease progresses through several stages, fatty liver, steatohepatitis, cirrhosis, and eventually, it leads to hepatocellular carcinoma (HCC) over a long period of time. Since a large proportion of patients with HCC are accompanied by cirrhosis, it is considered to be an important factor in the diagnosis of liver cancer. This is because cirrhosis leads to an irreversible harmful effect, but the early stages of chronic liver disease could be reversed to a healthy state. Therefore, the discovery of biomarkers that could identify the early stages of chronic liver disease is important to prevent serious liver damage. Biomarker discovery at liver cancer and cirrhosis has enhanced the development of sequencing technology. Next generation sequencing (NGS) is one of the representative technical innovations in the biological field in the recent decades and it is the most important thing to design for research on what type of sequencing methods are suitable and how to handle the analysis steps for data integration. In this review, we comprehensively summarized NGS techniques for identifying genome, transcriptome, DNA methylome and 3D/4D chromatin structure, and introduced framework of processing data set and integrating multi-omics data for uncovering biomarkers.

• Parasites, Hosts and Diseases
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• v.58 no.4
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• pp.475-479
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• 2020

Tegumental and excretory-secretory proteins are reported as diagnostic antigens for human opisthorchiasis. Rhophilin associated tail protein1-like (OvROPN1L) protein of Opisthorchis viverrini sperm tail showed potential as a diagnostic antigen. The OvROPN1L recombinant fragments were assayed for diagnostic antigenicity for human opisthorchiasis using indirect ELISA. The strongest antigenic region was a N-terminus peptide of M1 - P56. One synthetic peptide (P1, L3-Q13) of this region showed the highest antigenicity to opisthorchiasis. Sera from other parasitic infections including Strongyloides stercoralis, hookworm, Taenia spp, minute intestinal flukes, Paragonimus spp showed lower reactivity to P1. Peptide P1 is located in the disordered N-terminus of ROPN1L supporting its suitability as linear epitope. In the Platyhelminthes the N-terminal sequence of ROPN1L is diverging with taxonomic distance further suggesting that peptide P1 has potential as diagnostic tool in the genus Opisthorchis/Clonorchis. It should be further evaluated in combination with peptides derived from other O. viverrini antigens to increase its diagnostic power.

• Biomedical Science Letters
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• v.28 no.4
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• pp.260-275
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• 2022

Sterile alpha motif (SAM) domains bind to various proteins, lipids, and RNAs. However, these domains have not yet been analyzed as prognostic biomarkers. In this study, SAM domain containing 13 (SAMD13), a member of the SAM domain, was evaluated to identify a novel prognostic biomarker in various human cancers, including hepatocellular carcinoma (HCC). Moreover, we identified a correlation between SAMD13 expression and immune cell infiltration in HCC. We performed bioinformatics analysis using online databases, such as Tumor Immune Estimation Resource, UALCAN, Kaplan-Meier plotter, LinkedOmics, and Gene Expression Profiling Interactive Analysis2. SAMD13 expression in HCC samples was significantly higher than that in normal liver tissue; additionally, SAMD13 was higher in primary tumors, various stages of cancer and grades of tumor, and status of nodal metastasis. Higher SAMD13 expression was also associated with poorer prognosis. SAMD13 expression positively correlated with CD8+ T cells, CD4+ T cells, B cells, neutrophils, macrophages, and dendritic cells. In the analysis of SAMD13 co-expression networks, positively related genes of SAMD13 were associated with a high hazard ratio in different types of cancer, including HCC. In biological function of SAMD13, SAMD13 mainly include spliceosome, ribosome biogenesis in eukaryote, ribosome, etc. These results suggest that SAMD13 may serve as a novel prognostic biomarker for HCC diagnosis and provide novel insights into tumor immunology in HCC.

• Proceedings of the Korea Information Processing Society Conference
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• 2023.11a
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• pp.685-686
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• 2023

상수도관의 수질 및 부식도 검사에는 파이프에 손상을 입히지 않고 지속적인 방법이 필요하다. 초음파는 이를 만족하면서 상태를 확인할 수 있고 주파수가 높을수록 해상도가 좋아져 정밀한 측정이 가능하다는 장점이 있다. 이러한 특성을 이용해 상수도관 모니터링 시스템으로 초음파 기반의 Scanning Acoustic Microscopy(SAM)과 Convolutional Neural Network(CNN)을 사용하는 새로운 방법을 제안한다. 기존의 Non-Destructive Testing(NDT)방식의 단점을 보완하면서 더 높은 해상도로 상수도관을 점검하는 방식으로, SAM 을 이용하여 부식으로 인한 파이프 두께 변화와 부유물의 여부 및 수질을 동시에 감지하고 얻은 데이터를 CNN 으로 분석했다. CNN 의 높은 정확도 결과로 이 시스템의 파이프 부식도 및 수질 모니터링에 대한 적합성을 보여주었다.