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Evaluation of Rhophilin Associated Tail Protein (ROPN1L) in the Human Liver Fluke Opisthorchis viverrini for Diagnostic Approach

  • Geadkaew-Krenc, Amornrat (Graduate Program in Biomedical Sciences, Faculty of Allied Health Sciences, Thammasat University) ;
  • Grams, Rudi (Graduate Program in Biomedical Sciences, Faculty of Allied Health Sciences, Thammasat University) ;
  • Phadungsil, Wansika (Graduate Program in Biomedical Sciences, Faculty of Allied Health Sciences, Thammasat University) ;
  • Chaibangyang, Wanlapa (Graduate Program in Biomedical Sciences, Faculty of Allied Health Sciences, Thammasat University) ;
  • Kosa, Nanthawat (Graduate Program in Biomedical Sciences, Faculty of Allied Health Sciences, Thammasat University) ;
  • Adisakwattana, Poom (Department of Helminthology, Faculty of Tropical Medicine, Mahidol University) ;
  • Dekumyoy, Paron (Department of Helminthology, Faculty of Tropical Medicine, Mahidol University)
  • Received : 2019.12.24
  • Accepted : 2020.07.29
  • Published : 2020.08.31

Abstract

Tegumental and excretory-secretory proteins are reported as diagnostic antigens for human opisthorchiasis. Rhophilin associated tail protein1-like (OvROPN1L) protein of Opisthorchis viverrini sperm tail showed potential as a diagnostic antigen. The OvROPN1L recombinant fragments were assayed for diagnostic antigenicity for human opisthorchiasis using indirect ELISA. The strongest antigenic region was a N-terminus peptide of M1 - P56. One synthetic peptide (P1, L3-Q13) of this region showed the highest antigenicity to opisthorchiasis. Sera from other parasitic infections including Strongyloides stercoralis, hookworm, Taenia spp, minute intestinal flukes, Paragonimus spp showed lower reactivity to P1. Peptide P1 is located in the disordered N-terminus of ROPN1L supporting its suitability as linear epitope. In the Platyhelminthes the N-terminal sequence of ROPN1L is diverging with taxonomic distance further suggesting that peptide P1 has potential as diagnostic tool in the genus Opisthorchis/Clonorchis. It should be further evaluated in combination with peptides derived from other O. viverrini antigens to increase its diagnostic power.

Keywords

References

  1. Sripa B, Bethony JM, Sithithaworn P, Kaewkes S, Mairiang E, Loukas A, Mulvenna J, Laha T, Hotez PJ, Brindley PJ. Opisthorchiasis and opisthorchis-associated cholangiocarcinoma in Thailand and Laos. Acta Trop 2011; 120 (suppl): 158-168.
  2. Umesha KR, Kumar S, Parvathi A, Duenngai K, Sithithaworn P, Karunasagar I, Karunasagar I. Opisthorchis viverrini: detection by polymerase chain reaction (PCR) in human stool samples. Exp Parasitol 2008; 120: 353-356. https://doi.org/10.1016/j.exppara.2008.09.004
  3. Sato M, Pongvongsa T, Sanguankiat S, Yoonuan T, Dekumyoy P, Kalambaheti T, Keomoungkhoun M, Phimmayoi I, Boupha B, Moji K, Waikagul J. Copro-DNA diagnosis of Opisthorchis viverrini and Haplorchis taichui infection in an endemic area of Lao PDR. Se Asian J Trop Med 2010; 41: 28-35.
  4. Duenngai K, Boonmars T, Sithithaworn J, Sithithaworn P. Diagnosis of early infection and post chemotherapeutic treatment by copro-DNA detection in experimental opisthorchiasis. Parasitol Res 2013; 112: 271-278. https://doi.org/10.1007/s00436-012-3134-0
  5. Worasith C, Kamamia C, Yakovleva A, Duenngai K, Wangboon C, Sithithaworn J, Watwiengkam N, Namwat N, Techasen A, Loilome W, Yongvanit P, Loukas A, Sithithaworn P, Bethony JM. Advances in the Diagnosis of Human Opisthorchiasis: development of Opisthorchis viverrini antigen detection in Urine. PLoS Negl Trop Dis 2015; 9: e0004157. https://doi.org/10.1371/journal.pntd.0004157
  6. Teimoori S, Arimatsu Y, Laha T, Kaewkes S, Sereerak P, Sripa M, Tangkawattana S, Brindley PJ, Sripa B. Chicken IgY-based coproantigen capture ELISA for diagnosis of human opisthorchiasis. Parasitol Int 2017; 66: 443-447. https://doi.org/10.1016/j.parint.2015.10.011
  7. Jamornthanyawat N. The diagnosis of human opisthorchiasis. Southeast Asian J Trop Med Public Health 2002; 33 (suppl) : 86-91.
  8. Rattanachan S, Grams R, Tesana S, Smooker PM, Grams SV. Opisthorchis viverrini: analysis of the sperm-specific rhophilin associated tail protein 1-like. Acta Trop 2014; 140: 34-40. https://doi.org/10.1016/j.actatropica.2014.08.002
  9. Larsen JE, Lund O, Nielsen M. Improved method for predicting linear B-cell epitopes. Immunome Res 2006; 2: 1-7. https://doi.org/10.1186/1745-7580-2-1
  10. Xu KB, Qi HY. Sperm-specific AKAP3 is a dual-specificity anchoring protein that interacts with both protein kinase a regulatory subunits via conserved N-terminal amphipathic peptides. Mol Reprod Dev 2014; 81: 595-607. https://doi.org/10.1002/mrd.22329
  11. Carr DW, Fujita A, Stentz CL, Liberty GA, Olson GE, Narumiya S. Identification of sperm-specific proteins that interact with A-kinase anchoring proteins in a manner similar to the type II regulatory subunit of PKA. J Biol Chem 2001; 276: 17332-17738. https://doi.org/10.1074/jbc.M011252200
  12. Fiedler SE, Dudiki T, Vijayaraghavan S, Carr DW. Loss of R2D2 proteins ROPN1 and ROPN1L causes defects in murine sperm motility, phosphorylation, and fibrous sheath integrity. Biol Reprod 2013; 88: 41. https://doi.org/10.1095/biolreprod.112.105262
  13. Drozdetskiy A, Cole C, Procter J, Barton GJ. JPred4: a protein secondary structure prediction server. Nucleic Acids Res 2015; 43: W389-394. https://doi.org/10.1093/nar/gkv332
  14. Kelley LA, Mezulis S, Yates CM, Wass MN, Sternberg MJ. The Phyre2 web portal for protein modeling, prediction and analysis. Nat Protoc 2015; 10: 845-858. https://doi.org/10.1038/nprot.2015.053
  15. Ricciardi A, Ndao M. Diagnosis of parasitic infections: what's going on? J Biomol Screen 2015; 20: 6-21. https://doi.org/10.1177/1087057114548065