• 제목/요약/키워드: biomaterials

검색결과 2,096건 처리시간 0.031초

Enhancement of Immunomodulatory and Anticancer Activity of Fucoidan by Nano Encapsulation

  • Qadir, Syed Abdul;Kwon, Min-Chul;Han, Jae-Gun;Ha, Ji-Hye;Jin, Ling;Jeong, Hyang-Suk;Kim, Jin-Chul;You, Sang-Guan;Lee, Hyeon-Yong
    • Food Science and Biotechnology
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    • 제17권6호
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    • pp.1254-1260
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    • 2008
  • The aim of the present study was to prepare nanosample of fucoidan using lecithin as encapsulated material and to investigate the anticancer and immunomodulatory activity of nanoparticle in vitro. The nanoparticles have been characterized by dynamic light scattering (DLS) and transmission electron microscopy (TEM). Confocal microscopy confirmed the internalization of the fucoidan conjugates into the immune cells. The uptake of nanoparticles was confirmed with confocal microscopy demonstrating their localization in the cells. The anticancer activity was increased over 5-10% in different cancer cells of fucoidan nanoparticle as compare with fucoidan. The human B and T cells growth and the secretion of interleukin-6 and tumor necrosis factor-a from B cell were also improved by fucoidan nanoparticle because of the rapid absorption of nanoparticle into the cells as compare to fucoidan. At 0.6 mg/mL concentrations, the fucoidan nanoparticle showed better activity than 1.0 mg/mL concentration in T cell growth because the cells reached their saturation capacity. When the fucoidan was encapsulated in lecithin, its anticancer as well as its immunomodulatory activity proved to be superior from that of itself in pure form.

Chemotaxonomic Significance of Taxifolin-3-O-Arabinopyranoside in Rhododendron Species Native to Korea

  • Kim, TaeHee;Kwon, Ye Eun;Park, Sun Min;Kim, Min Seok;Jeong, Young Hwan;Park, Se Yeong;Bae, Young-Soo;Cheong, Eun Ju;He, Yi-Chang;Gong, Chun;Gao, Wei;Kim, Hee Kyu;Ham, Yeon Ho;Kim, Jin-Kyu;Choi, Sun Eun
    • Journal of Forest and Environmental Science
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    • 제38권3호
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    • pp.159-173
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    • 2022
  • Genus of Rhododendron has been used in traditional medicine since ancient times and is known to be effective in immune function, inflammation, and cold symptoms. And the reason for this activity is the flavanonol type among flavonoids in the genus of Rhododendron. Among the flavanonol types, Taxifolin-3-O-arabinopyranoside was isolated from the root of native R. mucronulatum in Korea, and the structure was finally identified through HPLC, LC-MS/MS, 1H-NMR, and 13C-NMR. Taxifolin-3-O-arabinopyranoside is a compound mainly found in R. mucronulatum, a representative species of the genus of Rhododendron, and exhibits antioxidant, anti-inflammatory, and anti-atopic activities. In this study, Taxifolin-3-O-arabinopyranoside was chemotaxonomic significant in 5 species of the genus Rhododendron native to Korea (R. mucronulatum, R. mucronulatum var. ciliatum, R. schlippenbachii, R. yedoense var. Poukhanense, R. japonicum for. Flavum). Compared with the existing literature, Taxifolin-3-O-arabinopyranoside was identified for the first time in 4 species of Rhododendron except for the R. mucronulatum.

Identification and Characterization of the Vibrio vulnificus rtxA Essential for Cytotoxicity in vitro and Virulence in Mice

  • Lee, Jeong-Hyun;Kim, Myung-Won;Kim, Byoung-Sik;Kim, Seung-Min;Lee, Byung-Cheol;Kim, Tae-Sung;Choi, Sang-Ho
    • Journal of Microbiology
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    • 제45권2호
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    • pp.146-152
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    • 2007
  • A mutant exhibiting decreased cytotoxic activity toward INT-407 intestinal epithelial cells and carrying a mutation in the rtx gene cluster that consists of rtxCA and rtxBDE operons was screened from a library of V. vulnificus mutants. The functions of the rtxA gene, assessed by constructing an isogenic mutant and evaluating its phenotypic changes, demonstrated that RtxA is essential for the virulence of V. vulnificus in mice as well as in tissue cultures.