• Korean Journal of Ecology and Environment
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• v.56 no.2
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• pp.151-160
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• 2023

Cyanobacteria Pseudanabaena strains are known to produce 2-MIB(odorous material) in freshwater systems, thereby causing problems in water use. However, their physiological responses to environmental factors in relation with 2-MIB production is not well explored. This study was conducted to evaluate the effect of temperature on the growth and 2-MIB production of Pseudanabaena redekei. The experimental cyanobacteria strains were separated from the Uiam Reservoir (North Han River) and cultured in the BG-11 medium. Temperature was set to 10, 15, 20, 25, and 30℃ for the experiment, in the reflection of the seasonal water temperature variation in situ. For each temperature treatment, cyanobacterial biomass(Chl-a) and 2-MIB concentration (intra-cellular and extra-cellular fractions) were measured every 2 days for 18 days. Both maximal growth and total 2-MIB production of P. redekei appeared at 30℃. While intra-cellular 2-MIB contents were similar (26~29 ng L^-1) regardless of treated temperatures, extra-cellular 2-MIB concentration was higher only in high temperature conditions (25~30℃), indicating that the extents of 2-MIB biosynthesis and release by P. redekei vary with temperature. The 2-MIB productivity of P. redekei was much higher in low-temperature conditions (10~15℃) than high temperature conditions (25~30℃). This study demonstrated that temperature was a critical factor contributing to 2-MIB biosynthesis and its release in cell growth (r=0.605, p<0.01). These results are important to understand the dynamics of 2-MIB in the field and thereby provide basic information for managing odorous material in drinking water resources.

• Journal of Environmental Science International
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• v.31 no.11
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• pp.911-922
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• 2022

To restore reclaimed land, it needs to be supplemented with organic matter; this is especially true for Korea, where organic matter constitutes only one-tenth of conventional agricultural soils. The giant Miscanthus, a perennial grass known for its extensive biomass, shows signs of being an excellent source of organic matter for restoring reclaimed land. Therefore, the objectives of this study were to (i) evaluate the feasibility of using the giant miscanthus as an organic resource within the context of re-using reclaimed land for agricultural purposes (i.e., potato cultivation), and (ii) determine the optimum fertilization rate for the potatoes while the giant miscanthus is being used as an organic resource. Our results show that after 180 days, giant miscanthus lost 23-47% of its original dry weight, with the extent of the loss dependent on soil salinity. Nutrient concentrations (Mg2+, Na+) continued to increase until the end of the study period. In contrast, potassium (K+) and the ratio of carbon to nitrogen (C/N) decreased until the end of the study period. Specifically, after 180 days, low salinity topsoil treatments had the lowest C/N ratio. In the first year, 150 % of standard N rates were required for the potatoes to achieve maximum productivity; however in the 2nd year, standard rates were sufficient to achieve maximum productivity. Overall, this implies that even though the application of giant miscanthus did eventually improve soil quality, increasing crop yields, N fertilization is still necessary for the best outcomes.

• Journal of Agricultural Extension & Community Development
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• v.11 no.2
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• pp.251-265
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• 2004

The agricultural sector's economic structure in Korea is regarded to encounter major barriers on the way toward revitalizing its economic prosperity. Among many, the energy-related problem is one of prime nuclei embedded in the country's agricultural sector. The ought-to-come structural changes in the country's agricultural energy system hinge upon the central government's policy direction as well as efforts of local governments and local farming community members. The indirect aids via 'cross subsidy' of electricity tariff rate and 'tax-exempt price' of oil fuels are two notable causes of the unsustainable energy consumption pattern in the country's agricultural sector. As measures, demand-side management(DSM) and energy-efficiency promotions are regarded to be the most attractive methods for energy conservation and economic productivity as well. Development of renewable energy sources are also receiving a great deal of attention for the long-term alternatives to the country's existing oil-based agricultural production mode. This study examines the contributive potential of DSM approaches and renewables-based technologies. With the critical evaluation on the concurrent adversities of the country's agricultural energy system, various sources of renewable energy-solar power, wind power, biomass, etc.-are examined for the purpose of technological and economical viability. As sufficient potentials of renewable energy sources are being estimated, both the system production cost and the installation cost for the county's rural areas are expected to lower in the long term. DSM options are also evaluated to be fruitful even in the short term. Both the public and civil arenas must galvanise each side's effort in order to promote these policy options and community potentials.

• Journal of Applied Biological Chemistry
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• v.50 no.1
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• pp.22-28
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• 2007

Most of the uplands in alpine regions during off-season are left as bare soil and thus vulnerable to severe erosion due to the inherent topographical conditions. Appropriate management strategy to cope with this problem is urgently needed, yet few researches have been reported on the effects of winter cover crop and management on soil erosion. We assessed effects of ryegrass (Lolium multiflorum) as cover crop, green manure or mulching residue on soil erosion and quality through field and segment plot lysimeter experiments in alpine uplands. Ryegrass successfully adopted to winter in alpine region based on biomass, nutrient contents, and vigors of top and root systems. Incorporation of ryegrass into soil maintained soil fertility, nutrient uptake, and yield of cabbage exerting potential use as green manure. Cultivation of ryegrass suppressed occurrence of Chinese cabbage pests. Surface coverage by ryegrass as cover crop and mulching residue significantly reduced soil loss up to 96%, when combined with soil conservation management practices. Results revealed maintaining cover crop over winter was beneficial in reducing soil erosion, and sustaining soil quality and Chinese cabbage productivity. This study suggested winter cover crop, followed by green manure and mulching, and conservation tillage system could be one of the best management practices in alpine sloping uplands cultivating Chinese cabbage.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.2
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• pp.185-191
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• 2012

This study was performed to screen fallow crops during winter period for improvement of soil quality and utilizing as mulching material in watermelon cropping system during winter period. Five fallow crops, mainly, hairy vetch, barley, rye, oat and wheat, were sown in early November. They were mowed for covering the soil surface instead of polyethylene (PE) film before watermelon planting in early April the following year. The highest absorbed nutrients and dry matter yield were found in rye. Bulk density in plots with fallow crop was lower than control plot. There was observed no significant differences among the fallow crops. However, porosity was the lowest in control plot. Soil EC reduced to 12%, 13%, 14%, 16% and 22%, respectively, by cultivation of hairy vetch, oat, wheat, barley and rye. Microbial biomass carbon and dehydrogenase activities were higher in soil treated with gramineous crops, such as barley, rye and oat. The growth of watermelon was more affected by regeneration of fallow crop than the occurrence of weed, especially in plots treated with rye or oat. Also, the fruit damage by aphid was found severe in these treatment plots. The fruit yield in plots treated with hairy vetch and barley was increased 5.7% and 2.6%, respectively, compared to that of PE films. The present experiment findings implied that these fallow crops had significant beneficial effects on improvement of soil qualities and could be utilized for mulching materials in watermelon cropping system.

• Journal of Mushroom
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• v.6 no.1
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• pp.27-31
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• 2008

The optimization of submerged culture conditions for mycelial growth and exopolysaccharide (EPS) production in an edible mushroom Tremella fuciformis were studied in shake flasks and bioreactors. The temperature of $28^{\circ}C$ and pH 8 in the beginning of fermentation in agitated flasks was the most efficient condition to obtain maximum mycelial biomass and EPS. The optimal medium constituents were as follows (g l-1): glucose 20, tryptone 2, $KH_2PO_4$ 0.46, $K_2HPO_4$ 1 and $MgSO_4H_2O$ 0.5. The fungus was cultivated under various agitation and aeration conditions in a 5L stirred-tank bioreactor. The maximum cell mass and EPS production were obtained at a relatively high agitation speed of 200 rpm and at an aeration rate of 2 vvm. The flow behavior of the fermentation broth was Newtonian and the maximum apparent viscosity (35 cP) was observed at a highly aerated condition (2 vvm). The EPS productivity in an airlift reactor was higher than that in the stirred-tank reactor. The EPS was protein-bound polysaccharides consisted of mainly mannose, xylose, and fructose. The molecular weights of EPS were determined to be $1.3{\sim}1.5{\times}10^6$.

• Korean Journal of Environmental Agriculture
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• v.38 no.4
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• pp.245-253
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• 2019

BACKGROUND: Weathering of bottom ash (BA) might induce change of its surface texture and pH and affect physical and chemical properties of soil associated with greenhouse gas emission, when it is applied to the arable soil. This study was conducted to determine effect of weathering of BA in mitigating emission of greenhouse gases from upland soil. METHODS AND RESULTS: In a field experiment, methane (CH₄), carbon dioxide (CO₂), and nitrous oxide (N₂O) emitted from the soil was periodically monitored using closed chamber. Three month-weathered BA and non-weathered BA were applied to an upland soil at the rates of 0, 200 Mg ha^-1. Maize (Zea mays L.) was grown from July 1_st to Oct 8_th in 2018. Both BAs did not affect cumulative CH₄ emission. Cumulative CO₂ emission were 23.1, 19.8, and 18.8 Mg/ha/100days and cumulative N₂O emission were 35.8, 20.9, and 17.7 kg/ha/100days for the control, non-weathered BA, and weathered BA, respectively. Weathering of BA did not decrease emission of greenhouse gases significantly, compared to the weathered BA in this study. In addition, both BAs did not decrease biomass yields of maize. CONCLUSION: BA might be a good soil amendment to mitigate emissions of CO₂ and N₂O from arable soil without adverse effect on crop productivity.

• The Korean Journal of Ecology
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• v.19 no.4
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• pp.341-351
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• 1996

In order to elucidate the differences in early successional development among similary aged old-fields having different soil nitrogen (N), caused by the land use history before at abandonment, the response of plant community along an experimental nitrogen gradient (control plot (No), plot NI with 5.8g $N/m^2$, plot N2 with 11.7g $N/m^2$ and plot N3 with 23.3g $N/m^2$) was investigated in a five-year-old abandoned field. Although the N content in soil among treatments was similar at the end of the growing season, N concentrations in plant tissue increased with the amount of N supplied. These results suggest that almost all the N contained in N-enriched soil might be absorbed by plants during the growing season after N supply. Vegetation tended to grow vigorously by nitrogen supply, and the standing biomass increased significantly in plots NI and N2 . Species richness of plants, especially of annuals and perennials, was more reduced than the control plot, and the species diversity was also reduced by N supply. The importance value (IV) of species by N supply differed in each species along the position on the successional sere: Artemisia princeps var. orientalis as the dominant species in this old-field decreased slightly; annuals as the earlier successional species decreased clearly along nitrogen gardients; Erigeron annuals as the earlier successional species and as a strong competitor with Artemisia princeps var. orientalis had the highest IV by small N supply; Miscanthus sinensis and Rubus crataegifolius as the later successional species increased by large N supply. These results suggest that old-fields with high soil N might show the structural and functional characteristics of the earlier successional stages, but community composition in those old-fields might be changed more quickly from the sarlier successional species than the later successional species.

• Korean Journal of Medicinal Crop Science
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• v.14 no.6
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• pp.371-375
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• 2006

To investigate effects on the growth and ginsenosides accumulation in ginseng hairy root, potassium phosphate was supplemented with 1.25, 2.5, and 5.0 mM concentration in 1/2 MS medium, respectively. Potassium phosphate supplement was increased the biomass and ginsenosides accumulation when it was dose at the concentration of 1.25 mM. And the growth rate of hairy root in the light condition was higher than in dark condition. The highest contents and productivity of ginsenosides were observed at the supplement of 1.25 mM potassium phosphate at the 7th day after the culture onset. Ginseng hairy root cultured in 20 L bioreactor supplemented with 1.25 mM KH$_2$PO$_4$ was increased the growth with 1,609 g (F${\cdot}$W) and ginsenosides content with 11.09 mg than those in control.

• Korean Journal of Medicinal Crop Science
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• v.12 no.6
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• pp.478-482
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• 2004

Suspension culture of Schisandra chinensis for production of gomisin J was perfomed in bioreactor. The inoculum size and initial sucrose concentration had significant effect on the cell growth and gomisin J accumulation. The maximum dry cell weight $(DCW;\;43.5\;g/{\ell})$ and gomisin J content $(0.71\;{\times}\;10^{-3}\;{\mu}g/g\;DCW)$ were obtained at inoculum size of 100 g fresh cell weight (FCW) per liter and MB5 medium containing 6% sucrose after 8 weeks of culture. The effect of oxygen supply on the cell growth and gomisin J accumulation was also investigated in an airlift-type bioreactor. The optimal cell growth and gomisin J content was obtained under 0.5 vvm. The productivity of gomisin J was 0.7 fold in bioreactor culture lower than that obtained in a flask cultivation.