• 한국컴퓨터그래픽스학회논문지
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• 제2권2호
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• pp.61-68
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• 1996

본 논문에서는 대용량의 DNA 염기 배열의 정성 정보를 특징짓기 위한 새로운 가시화 방법을 제안한다. DNA 배열은 배열 자체가 방대한 양의 정보를 포함하고 있기 때문에 분석에 많은 어려움이 있다. 우리는 DNA 염기 배열들사이의 상사성 비교를 위해 DNA 염기 배열을 하나의 이미지 도메인으로 변환한다. 프로그램은 random walk plot으로 DNA 염기 배열을 가시화한 후에 k-convex hull로 단순화 시킨다. Random Walk plot은 염기배열을 평면상에 하나의 커브로 표현한다. k-convex hull은 walk plot으로부터 무의미한 부분을 제거함으로서 walk plot을 단순화한다. 이러한 방법은 유전공학자들에게 쉽게 DNA 배열의 특징을 인식하고 분류할 수 있는 직관을 제공한다. 실제 게놈 데이터로 실험한 결과는 논문에서 제안하는 방법이 긴 DNA 염기배열들 사이의 유사성 분석을 위해 좋은 가시화 도구임을 보여준다.

• BMB Reports
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• 제44권2호
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• pp.107-112
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• 2011

We have developed a biologist-friendly, stand-alone Java GUI application, IdBean, for ID conversion. Our tool integrated most of the widely used ID conversion services that provide programmatic access. It is the first GUI ID conversion application that supports the direct merging as well as comparison of conversion results from multiple ID conversion services without manual effort. This tool will greatly help biologists who handle multiple ID types for the analyses of gene or gene product lists. By referring to multiple conversion services, the number of failed IDs can be reduced. By accessing ID conversion service online, it will potentially provide the most up-to-date conversion results. The application was developed in modular form; however, it can be re-packaged into plug-in form. For the development of a bioinformatics analysis tool, the module can be used as a built-in ID conversion component. It is available at http://neon.gachon.ac.kr/IdBean/.

• BMB Reports
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• 제45권2호
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• pp.120-125
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• 2012

We have developed a biologist-friendly, Java GUI application (GoBean) for GO term enrichment analysis. It was designed to be a comprehensive and flexible GUI tool for GO term enrichment analysis, combining the merits of other programs and incorporating extensive graphic exploration of enrichment results. An intuitive user interface with multiple panels allows for extensive visual scrutiny of analysis results. The program includes many essential and useful features, such as enrichment analysis algorithms, multiple test correction methods, and versatile filtering of enriched GO terms for more focused analyses. A unique graphic interface reflecting the GO tree structure was devised to facilitate comparisons of multiple GO analysis results, which can provide valuable insights for biological interpretation. Additional features to enhance user convenience include built in ID conversion, evidence code-based gene-GO association filtering, set operations of gene lists and enriched GO terms, and user -provided data files. It is available at http://neon.gachon.ac.kr/GoBean/.

• Journal of Pharmaceutical Investigation
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• 제34권1호
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• pp.73-79
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• 2004

The union of information-technology and biology presents great possibilities to both applications of bio-information and development of science and technology. Also, meaningful analysis of bio-information brings about a new innovation in the field of bio-market with the advent and growth of bioinformatics. Hence, bioinformatics is the most import aspect for establishing a science-technology-oriented society in the $21^{st}$ century. This article provides trends in current state of bioinformatics. Technological development of bioinformatics for the rapid growth of bio-industry means that using bioinformatics, a biologist can process and store enormous amount of data such as current Human Genome Project and future data in the field of biology. We have manly looked at the tends of bio-information, databases and mining tools that are generally used, and strategies and directions for the future.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2006년도 제37회 하계학술대회 논문집 D
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• pp.2171-2172
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• 2006

Magnetic nerve stimulation treatment is much backward real condition than other field. Specially, successful medical treatment introduction of magnetic field (MF) can was refered long ago in Avicenna's work, and is thought as age of medicine magnetology development recently. These development is achieved through biologist and biophysicist and clinician's joint effort, but, new mountings and relationship air tassel are developed steadily. Magnetic nerve stimulation treatment field designs treatment system by each function during long wave high-amplitude (traditional magneto therapy of greatly great that strong that) short time that CMF, VMF, PMF field etc. are representative but are HPMT technology in this research and manufacture and special quality did comparative analysis.

• 한국컴퓨터산업학회논문지
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• 제7권3호
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• pp.263-270
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• 2006

자계신경 자극치료는 다른 분야에 비해 많이 낙후된 실정이다. 특히, 자기장(MF)의 성공적인 의료도입이 Avicenna의 연구에서 오래전에 언급되었고, 최근에 의학 magnetology 발달의 시대로 생각될 수 있다. 이러한 발달은 공학자와 생물학자 그리고 임상의의 접합부분 노력을 통하여 이루어지고 있으며, 새로운 장비들과 BT기술은 꾸준히 발전되고 있다. 자계신경 자극치료분야는 CMF, VMF, PMF분야 등이 대표적이나 본 연구에서는 HPMT기술인 장파 high-amplitude(전통적인 magneto-therapy의 크게 대단한 강렬) 짧은시간 치료용 시스템을 각 기능별로 설계하고 제작하여 특성을 비교분석 하였다.

• 한국독성학회:학술대회논문집
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• 한국독성학회 1998년도 국제심포지움 및 추계학술대회
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• pp.8-13
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• 1998

Polyoma Virus Enhancer core Binding Protein (PEBP2)는 유전자의 전사를 조절하는 hetero-dimeric transcription factor로서 $\alpha$와 $\beta$ subunit으로 구성되어 있다. $\alpha$ subunit을 coding 하는 유전자중 하나인 PEBP2aB는 급성백혈병과 관련되어있는 t(8;21) 또는 t(12;21)에 의하여 변형됨으로서 백혈병 발병의 원인이 되고 있다 (Miyoshi et al., 1993; .Romana et al., 1995). $\beta$ subunit을 coding 하는 PEBP2$\beta$도 inv(16)에 의하여 변형됨으로서 백혈병을 유도하는 주요 원인이 되고 있다 (Liu et al., 1993). 이 유전자들의 생물학적 활성을 밝히기 위한 연구가 gene targeting에 의한 knockout mouse 생산 방법으로 수행되었다. 그 결과 PEBP2$\alpha$B와 PEBP2$\beta$ 유전자가 definitive hematopoiesis에 있어서 결정적으로 중요한 역할을 하고 있음이 관찰되었다 (Okuda et al., 1996, Wang et al., 1996a, 1996b), 이는 이들 유전자가 bematopoietic master switch 유전자임을 밝힌 중요한 결과로서 이로부터 혈액학 연구 분야의 새로운 장이 열리게 되었다. 또한 이러한 연구 결과들은 PEBP2 family에 속하는 다른 유전자의 생물학적 활성의 연구를 촉진하는 계기가 되었다. 최근 PEBP2$\alpha$A 유전자가 결손된 마우스가 생산되었는데 이 유전자의 경우에는 모든 종류의 뼈의 생성이 완전히 결손됨이 관찰되었다 (Komori et al., 1997). 이는 PEBP2$\alpha$A 유전자가 뼈의 생성을 지배하는 master switch 유전자임을 보여주는 중요한 관찰로서 bone biologist 들의 큰 관심을 모으고 있다. 본 연구팀은 PEBP2 family 유전자 중 유일하게 아직 생물학적 활성이 규명되지 않은 PEBP2$\alpha$C 유전자의 활성을 knockout 마우스를 생산하는 방법에 의하여 분석하였으며 소화기관의 형성에 중요한 역할을 하고 있음을 확인하였다.

• Journal of Microbiology and Biotechnology
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• 제17권4호
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• pp.547-559
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• 2007

Bacillus thuringiensis (Bt) was first described by Berliner [10] when he isolated a Bacillus species from the Mediterranean flour moth, Anagasta kuehniella, and named it after the province Thuringia in Germany where the infected moth was found. Although this was the first description under the name B. thuringiensis, it was not the first isolation. In 1901, a Japanese biologist, Ishiwata Shigetane, discovered a previously undescribed bacterium as the causative agent of a disease afflicting silkworms. Bt was originally considered a risk for silkworm rearing but it has become the heart of microbial insect control. The earliest commercial production began in France in 1938, under the name Sporeine [72]. A resurgence of interest in Bt has been attributed to Edward Steinhaus [105], who obtained a culture in 1942 and attracted attention to the potential of Bt through his subsequent studies. In 1956, T. Angus [3] demonstrated that the crystalline protein inclusions formed in the course of sporulation were responsible for the insecticidal action of Bt. By the early 1980's, Gonzalez et al. [48] revealed that the genes coding for crystal proteins were localized on transmissible plasmids, using a plasmid curing technique, and Schnepf and Whiteley [103] first cloned and characterized the genes coding for crystal proteins that had toxicity to larvae of the tobacco hornworm, from plasmid DNA of Bt subsp. kurstaki HD-1. This first cloning was followed quickly by the cloning of many other cry genes and eventually led to the development of Bt transgenic plants. In the 1980s, several scientists successively demonstrated that plants can be genetically engineered, and finally, Bt cotton reached the market in 1996 [104].

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 2001년도 Proceedings of 2001 International Symposium
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• pp.15-19
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• 2001

Creating an artificial strain with a minimal gene set for a specific purpose is every biologist's dream. With the complete genome sequencing of more than 50 microorganisms and extensive functional analyses of their genes, it is possible to design a genetic blueprint for a simple custom-designed microbe with the minimal gene set. Two different approaches are being considered. The first 'top-down' approach is trimming the genome to a minimal gene set by selectively removing genes of an organism thought to be unnecessary based on microbial genomics. The second 'bottom-up' approach is to synthesize the proposed minimal genome from basic chemical building blocks. The 'top-down' approach starting with the genome of a well known microorganism is more technically feasible, whereas the bottom-up approach may not be attainable in the nearest future because of the lack of the complete functional analysis of the genes needed for a life. Here in this study, we used the top-down approach to minimize the E. coli genome to create an artificial organism with 'core' elements for self-sustaining and self-replicating cells by eliminating unnecessary genes. Using several different kinds of sophisticated deletion techniques combined with a p:1age and transposons, we deleted about 19％ of the E. coli genome without causing any damages to cellular growth. This smaller E. coli genome will be further reduced to a genome with a minimal gene l;et essential for cell life. This minimized E. coli genome can lead to the construction of many custom-designed strains with myriad practical and commercial applications.

• 한국축산식품학회지
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• 제23권2호
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• pp.186-192
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• 2003

최근 항산화제 연구는 식품, 의약품, 농업분야 등 다방면에서 이용될 수 있기 때문에 많은 산업적 효과를 기대할 수 있다. 특히 지금까지 알려진 항산화제가 약한 활성, 독성 및 사용상의 한계로 인하여 사용하는데 있어서 많은 문제점을 내포하고 있다. 따라서 천연으로부터 보다 안전하고 강한 활성을 지닌 천연항산화제의 개발이 요구된다. 활성산소 제거능력이 향상된 유산균은 식품산업에 중요하며 인간 장내 외인성, 내인성 산화적 스트레스 제거에 중요하다고 생각된다. 따라서 유산균을 이용한 항산화제의 고부가가치 창출을 위해서는 생물학적 기능연구 및 질병모델계에서의 효능평가가 이루어져야 하며 항산화제의 효능검정 및 항산화제 작용 메커니즘 등 다양한 방면의 연구가 병행되어야 할 것으로 생각된다