• Journal of Biomedical Engineering Research
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• v.33 no.2
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• pp.72-77
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• 2012

Phase Contrast MR Angiography(PC MRA) is excellent MRA technique for measuring the velocity of vessels in the human body. PC MRA need to at least four images for angiogram reconstruction and it caused longer scan time. Therefore, we used keyhole imaging combined PC MRA to reduce the scan time. However, keyhole imaging can lead the erroneous effects as loss of phase information or frequency discontinuous. In this study, we applied the keyhole imaging combined 2D PC MRA for improving the temporal resolution and also measured the velocity to evaluate the accuracy of phase information. We used 0.32T MRI scanner(Magfinder II, Scimedix, Korea). Using the 2D PC MRA pulse sequence, the vascular images for a human brain targeted on the Superior Sagittal Sinus(SSS) were obtained. We applied tukey window function for keyhole images to minimize the ringing artifact and erroneous factors that are induced frequency discontinuous and phase information loss. We also applied zero-padded algorithm to peripheral missing k-space lines to compare keyhole imaging results and the artifact power(AP) value was measured on the complex difference images to validate the image quality. Consider as based on our results, heavy image distortions and artifacts were shown until using at least 50% keyhole factor. Using above the 50% keyhole factors are shown well reconstructed and matched for magnitude images and velocity information measurements. In conclusion, we confirmed the image quality and velocity information of keyhole technique combined 2D PC MRA. Especially, measured velocity information through the keyhole imaging combination was similar to the velocity information of full sampled k-space image despite of frequency discontinuous and phase information loss in the keyhole imaging reconstruction process. Consequently, the keyhole imaging combined 2D PC MRA will give some clinical usefulness and advantages as improving the temporal resolution and measuring the velocity information via selecting the appropriate keyhole factor at low tesla MRI system.

• Korean Journal of Plant Taxonomy
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• v.31 no.4
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• pp.321-341
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• 2001

Phytoliths of leaf blades of Oryza were studied by light and scanning electron microscopy in order to assign the diagnostic character and taxonomic key for the genus. Some phytoliths such as stomatal apparatus, long-cell and short-cell, existing at the same position on the abaxial side of leaf blade, were intensively investigated because of their various forms documented in a previous study. These characters have value either for testing infrageneric classification or for identifying taxa within the genus. Stomatal phytolith is formed by integration of several kinds of sources, such as the guard and subsidiary cell and the papillae. The stomatal phytolith, characterized by not only the absence and presence of phytolith originated by the papillae developed on the guard and subsidiary cell but also their pattern of arrangement, shows various morphologies, and these features have congruent with the infrageneric classification such as section and/or series. Long-cell phytolith is characterized by the absence/presence, arrangement and morphology of phytoliths originated by the papillae on the cell surface. These features may hardly have any systematic relevance within the genus, but contain some informations for identifying of species. All of short-cell phytoliths found are silica body. They form various shapes like cross, bilobate, saddlelike and trilobate, and these features are consistent with infrageneric classification such as section and/or series. Also, some characters, the absence/presence of band of short cell phytolith within costal strip, the absence/presence of saddlelike phytolith within intercostal strip and the number of band of short cell phytolith within costal strip are various according to taxa, but these features do not fall into infrageneric classification. Some taxonomic keys on the phytoliths of stomatal, long-cell and short-cell were developed by their features, and the agreement between these characters and infrageneric classifications was also discussed.

• Journal of Society of Preventive Korean Medicine
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• v.20 no.1
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• pp.145-159
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• 2016

Objectives : The aim was to investigate the characteristics of low dose effect and non monotonic dose responses(NMDRs) and to predict the influence it might have on the health and medicine, traditional Korean medicine. Methods : By investigating recently published major literatures related to low dose effect and NMDRs, the definition, mechanism, major related fields, and the influence on public health and medicine has been categorized and summarized. Results : Although there are still heated debates about the definition of low dose, it implies the biological responses in environmental exposure level and the NDMDRs means the nonlinear relationship between the dose-response in the slope sign change. Also, it implies the new form of the curve showing a U, reverse U shape, and the slop sign constantly changing showing various forms. This mechanism is because the two acceptor shows opposing effects to toxic materials and the affinity is different along with the numerical value that increase and decrease being different. These characteristics generally appear in endocrine disrupters such as bisphenol A, agricultural pesticides, metal, and radiation. The research field in the public health and medical treatment is obesity, problems in metabolism, growth hormone treatment, climacteric treatment, breast cancer, intake of Korean traditional medicine for pregnancy, menopause and phytoestrogen. Conclusions : As a result of discussing implications, NMDRs is a particular effect in low dose and heated debates surround this response, research is being conducted surrounding the field of obesity, problems in metabolism, growth hormone treatment, climacteric treatment, breast cancer, intake of Korean traditional medicine for pregnancy, menopause, and phytoestrogen. More research and interest in needed as it can have a massive influence in the public health and medicine.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.60 no.2
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• pp.239-247
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• 2015

This experiment was conducted to obtain the have higher contents of pharmaceutical constituents as well as higher yield from colchicine induced diploid and tetraploid extracts of Platycodon grandiflorum. In order to determine the biological activity, this study was focused to evaluate the cytotoxicity, antimicrobial on the bronthus disease bacteria, antioxidant enzyme activity of diploid and tetraploid extracts in P. grandiflorum. The activities of antioxidant enzyme according to different solvent extracts were measured as superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX). The cytotoxicity of methanol extracts of P. grandiflorum showed significant differences between tetraploid and diploid. That is, the cytotoxic effect against human cancer cell was higher in tetraploid than in diploid. At all extracts concentration, tetraploid samples showed high toxicity and the $IC_{50}$ (concentration causing 50% cell death) value showed the highest on HCT-116 cell ($105.91{\mu}g/mL$), and exhibited significant activity against the Hep 3B cell ($140.67{\mu}g/mL$), SNU-1066 cell ($154.01{\mu}g/mL$), Hela cell ($158.37{\mu}g/mL$), SNU-601 cell ($182.67{\mu}g/mL$), Calu-6 cell ($190.42{\mu}g/mL$), MCF-7 cell ($510.19{\mu}g/mL$). Antimicrobial activities of diploid P. grandiflorum were relatively low compared to tetraploid P. grandiflorum on most of the bacterial strains. In tetraploid P. grandiflorum, K. pneumoniae showed the clear zone formation (18~19 mm) of growth inhibition, followed by the clear zone formation of 13~15 mm on C. diphtheria and S. pyogenes. The antimicrobial activities in diploid P. grandiflorum were the highest on K. pneumonia (14~15 mm), and showed the clear zone formation of 11~12 mm on C. diphtheria and 12~13 mm on S. pyogenes. The antimicrobial activity is thought to look different depending on the bacterial strains and the polyploidy of P. grandiflorum. The root extract of P. grandiflorum had the highest (97.2%) SOD enzyme activity in ethyl acetate partition layer of tetraploid while water partition layer of diploid showed the lowest (48.6%) SOD enzyme activity. The activity of CAT showed higher values in the root of tetraploid than in the diploid of P. grandiflorum in all partition layers except butyl alcohol. The activities of APX and POD showed higher values in the root of tetraploid than in the diploid of P. grandiflorum in all fraction solvents except water layer. These results indicate that the tetraploid P. grandiflorum can be used as a source for developing cytotoxic agent and antimicrobials which can act against bronchus diseases bacterial strains.

• Applied Chemistry for Engineering
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• v.21 no.4
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• pp.372-376
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• 2010

Non-conventional energy is considered as important future energy source, as conventional energy has limitation for its capacity. The demand on value added process in heavy oil/oil sand bitumen is increasing in particular. Solvent Deasphalting (SDA) process for Deasphalted Oil (DAO) is used as heavy oil upgrading process in existing refinery process. SDA process for heavy oil upgrading has been already commercialized by leading countries. SDA R&D projects have been carried out actively by those countries. In this study, patent analysis for SDA technology development was carried out. From 1970's, when SDA patents were applied, the patents in Korea, USA, Japan, Canada and Europe were searched and distributed to extraction, recovery, solvent and etc. 334 patents were selected relating to heavy oil upgrading SDA process. The application status of SDA process patents showed a tendency to increase slightly. The number of patent applied was USA patent 131 (39%), Canada patent 83 (25%), Japan patent 35 (11%) and Korea patent 6 (2%). It will be necessary for efficient use of energy resource to support SDA R&D by government.

• Journal of Life Science
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• v.30 no.3
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• pp.278-284
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• 2020

Magnolia kobus is known to exert various biological effects, such as antioxidant and hypnotic activity. In this study, we investigated the antimicrobial and anti-inflammatory activity of M. kobus essential oil extracted using steam distillation. Its antimicrobial activity was tested against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa by the paper disk diffusion and minimum inhibitory concentration (MIC) methods. Its anti-inflammatory activity was evaluated by measuring its inhibition ratio on the production of nitric oxide (NO) and PGE₂ in lipopolysaccharide (LPS)-induced RAW264.7 cells. Its composition was analyzed by gas chromatography-mass spectrometry (GC-MS). The results showed that M. kobus essential oil exhibited excellent antibacterial activity against S. aureus, with a clear zone of 18 mm and an MIC value of 0.25 mg/ml. Its clear zones against P. aeruginosa and E. coli were 14 mm and 17 mm, respectively, while its MIC values were 1 mg/ml and 0.5 mg/ml, respectively. The essential oil exhibited no cytotoxicity to the RAW264.7 cells at a concentration of 500 ㎍/ml while showing NO (37.7%) and PGE₂ inhibition (24.0%). Its three main fragrance ingredients identified were 3-carene (77.07%), β-elemene (6.92%), and caryphyllene (2.86%). The results suggest that M. kobus essential oil has potential as a cosmetic functional material with antimicrobial and anti-inflammatory effects.

• Korean Journal of Ecology and Environment
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• v.50 no.2
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• pp.207-215
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• 2017

Body scales directly exposed to external environments can be an important factor to understand various characteristics of a species such as habitat features, life history and basic ecology. In this study, we compared size and morphology of dorsal, outermost dorsal, keeled dorsal and ventral scales of total nine snake species in Korea; Oocatochus rufodorsatus, Elaphe dione, Rhabdophis tigrinus, Amphiesma vibakari, Dinodon rufozonatum, Hierophis spinalis in the Colubridae and Gloydius ussuriensis, G. brevicaudus, G. saxatilis in the Viperidae. The morphological characteristics of the scales seem to well reflect foraging modes and moving activity of both families. Uniquely D. rufozonatum had a diamond shape dorsal scale and had the greatest and smallest value of the ratio of width/length of dorsal and ventral scales, respectively. O. rufodorsatus, D. rufozonatum and H. spinalis did not have keeled dorsal scales and E. dione had keel on the few of dorsal scales. In addition, morphological characteristics of scales of three viper species were closely consistent with previously known phylogenetic relationships.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.59 no.4
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• pp.391-397
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• 2014

Anthocyanins are water-soluble plant pigments that give rise to the red, purple, or blue colors observed in many crops. Especially purple pigment of colored rice represent biological activities has been an increased interest, due to the functional meal as a staple food. These health benefits have been attributed to antioxidant property of anthocyanin. However, there have been little genetic source for development of new and various colored rice variety. A recently developed new variety, 'Jungmo1020', showed an unique characteristic of blue color of rice grain hull. We identified the pigment petunidin-3-O-glucoside (Pt-3-G) as a major compound with a value of $176.3{\pm}2.3mg/100g$ (68.3%) from 80% MeOH with 1% HCl extracts by UPLC/MS/MS. The content of Pt-3-G in the extracts using a solvent of 100% MeOH with 1% HCl was the highest with the values of $183.8{\pm}2.5mg/100g$. In addition, these extracts showed excellent antioxidatative activities by DPPH and ABTs assay.

• Culinary science and hospitality research
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• v.21 no.3
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• pp.40-52
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• 2015

This study investigated the quality characteristics of cookies containing Kale powder, which is well known for its various functions and biological activity. In this study, we assessed the quality characteristics and antioxidant activity of cookies containing various concentrations (0, 3, 5, 7 and 9%) of kale powder(KP). To analyze quality characteristics, density and pH of dough, spread factor, moisture content, color(L, a, b), hardness, DPPH radical scavenging activity and sensory properties were measured. The spread factor and DPPH radical scavenging activity of the cookies significantly increased with increasing concentrations of KP, whereas pH and density of the dough, L value of the cookies significantly decreased. The results of sensory properties showed that cookies made with 3% and 5% KP did not differ significantly from the control in color, taste, and overall quality. Texture scores for the 3% and 5% KP groups ranked significantly higher than those of the other groups. Taken together, the results of this study suggest that KP is a beneficial ingredient for increasing the consumer acceptability and functionality of cookies. The quality characteristics of the 3% and 5% added samples exhibited significantly similar or higher values as compared to those of the controls, raising the possibility of developments in health-functional cookies. The results of the sensory evaluation produced very significant values for color, appearance, texture, and overall quality of instrumental analysis.

• Journal of The Korean Society of Grassland and Forage Science
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• v.19 no.1
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• pp.11-16
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• 1999

This study was conducted to compare the dry matter intake, the digestibility, and utilization of nitrogen and energy of Korean native goats, when fed on orchardgrass(OG) mixed with alfalfa(AA) at a different levels. Experimental diets include three treatments : OG 100%, OG 80%+AA 20%, and OG 60%+AA 40%. With increasing levels of AA supplementation, the content of CP was higher for AA supplemented diets than for OG 100% diets, while the content of NDF, ADF, hemicellulose and lignin content were slightly lower for AA supplemented diets than for OG 100% diets. Also, the DM intake and the digestibility of DM, cellular constituents, NDF and ADF were higher for AA supplemented diets than for OG 100% diets, especially those were the highest in OG 60%+AA 40% diets(P<0.05). The apparently digested N, retained N and biological value of AA supplemented diets were increased with increasing levels of AA supplementation, compared with those of OG 100% diets(P<0.05). The utilization of DE and ME were slightly high in OG 60%+AA 40% diets, but there was no significant difference among all diets. According to the results, DM intake, digestibility and nitrogen utilization were improved in goat fed diets with supplementation AA(AA 20%, 40%), compared with those of OG 100% diets. But energy utilization did not show the difference among diets. Hence, It needs to provides to the adequate energy sources to goats.