• KIPS Transactions on Software and Data Engineering
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• v.5 no.6
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• pp.267-272
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• 2016

Microscope cell image is an important indicator for obtaining the biological information in a bio-informatics fields. Since human observers have been examining the cell image with microscope, a lot of time and high concentration are required to analyze cell images. Furthermore, It is difficult for the human eye to quantify objectively features in cell images. In this study, we developed HCS algorithm for automatic analysis of cell image using an OpenCV library. HCS algorithm contains the cell image preprocessing, cell counting, cell cycle and mitotic index analysis algorithm. We used human cancer cell (MKN-28) obtained by the confocal laser microscope for image analysis. We compare the value of cell counting to imageJ and to a professional observer to evaluate our algorithm performance. The experimental results showed that the average accuracy of our algorithm is 99.7%.

• The Plant Pathology Journal
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• v.27 no.1
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• pp.59-67
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• 2011

A bacterial strain CH-67 which exhibits antagonism towards several plant pathogenic fungi such as Botrytis cinerea, Fulvia fulva, Rhizoctonia solani, Sclerotinia sclerotiorum, Colletotrichum sp. and Phytophthora sp. was isolated from forest soil by a chitin-baiting method. This strain was identified as Burkholderia cepacia complex (Bcc) and belonging to genomovar IX (Burkholderia pyrrocinia) by colony morphology, biochemical traits and molecular method like 16S rRNA and recA gene analysis. This strain was used to develop a bio-fungicide for the control of tomato leaf mold caused by Fulvia fulva. Various formulations of B. pyrrocinia CH-67 were prepared using fermentation cultures of the bacterium in rice oil medium. The result of pot experiments led to selection of the wettable powder formulation CH67-C containing modified starch as the best formulation for the control of tomato leaf mold. CH67-C, at 100-fold dilution, showed a control value of 85% against tomato leaf mold. Its disease control efficacy was not significantly different from that of the chemical fungicide triflumidazole. B. pyrrocinia CH-67 was also effective in controlling damping-off caused by Rhizoctonia solani PY-1 in crisphead lettuce and tomato plants. CH67-C formulation was recognized as a cell-free formulation since B. pyrrocinia CH-67 was all lethal during formulation process. This study provides an effective biocontrol formulation of biofungicide using B. pyrrocinia CH-67 to control tomato leaf mold and damping-off crisphead lettuce and tomato.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.44 no.4
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• pp.69-76
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• 2012

The bamboo fibers from three Korean bamboo species, Moso, Henon and Timber bamboo, were prepared by disintegration after thermal treatment. The samples were prepared according to the age of growth; bamboo shoots, 20 days and 50 days of growth. The fiber quality was also investigated by morphological and chemical analyses. There was no big difference in the dry mass of fibers among the three bamboo species. However, the dry mass of fibers from 50 days of growth was increased by 34%, whereas it was ranged in 9-20% in the case of fibers from bamboo shoots and 20 days of growth. In the results of morphological analysis, the fibers could be classified as the fibers from metaxylem, the fibers from parenchyma and the fines produced during disintegration. The fibers from 50 days of growth were separated from metaxylem, whereas the fibers from bamboo shoots and 20 days of growth were mainly consisted of fibers from metaxylem and parenchyma. The chemical analysis of fibers showed that the contents of carbohydrates, lignin and extractives were not much affected by thermal treatment.

• Korean Journal of Soil Science and Fertilizer
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• v.47 no.4
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• pp.299-305
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• 2014

In this paper, fractional factorial screening design (FFSD) and central composition design (CCD) were used to optimize the medium components for producing chitinase and gelatinase by Lysobacter capsici YS1215. Crab shell powder, nutrient broth and gelatin were proved to have significant effects on chitinase and gelatinase activity by FFSD first. An optimal medium was obtained by using a three factor CCD, which consisted of nutrient broth of $2.0gL^{-1}$, crab shell powder of $2.0gL^{-1}$ and gelatin of $1.0gL^{-1}$, respectively with the highest chitinase activity ($3.34UmL^{-1}$) and gelatinase activity ($14.15UmL^{-1}$). This value was 3.76 and 1.11 fold of the chitinase and gelatinase activity, respectively, compared to the lowest productive medium in the design matrix. In investigating potential of these enzymes partially purified from L. capsici YS1215 for biotechnological use, the crude enzymes was found to be inhibition against pathogenic fungal mycelia: Colletotrichum gleosporioides, Phytophthora capsici, and Rhizoctonia solani. In this study, we demonstrated the optimal medium for producing the chitinolytic and gelatinolytic enzymes by the strain YS1215 and the role of their enzymes that may be useful for further development of a biotechnological use and agricultural use for biological control of phytopathogenic fungi.

• Korean Journal of Microbiology
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• v.38 no.3
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• pp.180-185
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• 2002

The direct viable count (DVC) and plate count (PC) methods was used to measure the number of bacteria in potable groundwater samples collected from bottled water from the market, mineral water, and edible groundwater near the urban areas and the stock farming congested areas. As a result, the number of living bacteria by DVC was comprised 30~80% of the total direct count (TDC), whereas the number of living bacteria by PC was around l~30% of DVC. Such results show that viable but non-culturable (VBNC) bacteria exist in the potable groundwater with high percentages. On the other hand, upon measuring the value from the conventional nutrient broth (NB), $10^-2$ fold diluted nutrient broth (DNB), and R2A broth, the values from the DNB and R2A showed 2~50 times higher than the conventional NB medium. These results indicate that oligotrophic bacterial groups which can multiply in the low nutrient broth abundantly exist in the oligotrophic environment like potable groundwater.

• Korean Journal of Microbiology
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• v.36 no.3
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• pp.221-227
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• 2000

This study was performed to investigate the biological characteristics of twenty isolates of extended spectnlm $\beta$-lactamase (ESBL) producing Klebsiellapnezm~onia collected kom the various clinical specimens of three hospitals in Pusan. Isoelectric focusing (IEF) and PCR were used to determine the types of $\beta$-lactamase gene in this study. Twenty isolates of ESBL producing Klebsiellnp~ieun~or~iae could be divided by PCR, such as TEM type (I1 strains), SHV type (8 strains); non TEM non SHV type (1 strain). In the isoelechic focusing test, the PI of TEM type was 5.2-6.0 and that of SHV type was 6.9-7.4. According to the pI value and PCR bands, twenty strains of ESBL Klebsiellapneumoniae were divided into 5 types: TEM type @I 5.2-6.0; 1080 bp on PCR band), TEM + SHV type (pI 5.2-6.0; andpI 7.0-7.4; 1080 bp and 599 bp on PCR band), SHV type (pl7.0-7.4; 599 bp on PCR band), non TEM non SHV type, and otber type (PCR result was SHV type but pI was not detected).

• Journal of Nutrition and Health
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• v.38 no.7
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• pp.503-511
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• 2005

In this study we investigated the biological activity of Chondria Crassicaulis (CC) on the human cancer cells. CC was extracted with methanol and further fractionated into four different types: hexane (CCMH), methanol (CCMM), butanol (CCMB), and aqueous (CCMA) partition layers. We determined the cytotoxic effect of these layers on human cancer cells by MTT assay. Among various partition layers of CC, the CCMM and CCMB showed the strong cytotoxic effects at 150 ${\mu}g$/ml which resulted $98.91\%$, $92.96\%$ on HeLa cell lines and $95.47\%$, $77.05\%$ on MCF-7 cell lines. And, the anti-proliferative effect of CC was accompanied by a marked inhibition of cyclooxygenase (COX-2), Caspase-3 and IAP (cIAP-1, cIAP-2 and XIAP) protein and concomitant induction of p53, p21 and Survivin protein. However, CC did not affect the level of Bax, Bcl-2 and Bcl-XL- protein. Also, we observed quinone reductase (QR) induced effects in all fraction layers of CC on HepG2 cells. The QR induced effects of the CCMH and CCMM on HePG2 cells at 120 ${\mu}g$/mL concentration indicated 3.73 and 2.45 with the control value of 1.0. Although further studies are needed, the present work suggests that CC may be a chemopreventive agent for the treatment of human cancer cells.

• Journal of The Korean Society of Agricultural Engineers
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• v.61 no.6
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• pp.1-8
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• 2019

Carbon dioxide is one of the major driving forces causing climate changes, and many countries have been trying to reduce carbon dioxide emissions from various sources. Soil stores more carbon dioxide(two to three times) amounts than atmosphere indicating that soil organic carbon emission management are a pivotal issue. In this study, we developed a Soil Organic Carbon(SOC) storage estimation model to predict SOC storage amounts in soils. Also, SOC storage values were assessed based on the carbon emission price provided from Republic Of Korea(ROK). Here, the SOC model calculated the soil hydraulic properties based on the soil physical and chemical information. Base on the calculated the soil hydraulic properties and the soil physical chemical information, SOC storage amounts were estimated. In validation, the estimated SOC storage amounts were 486,696 tons($3.526kg/m^2$) in Jindo-gun and shown similarly compared to the previous literature review. These results supported the robustness of our SOC model in estimating SOC storage amounts. The total SOC storage amount in ROK was 305 Mt, and the SOC amount at Gyeongsangbuk-do were relatively higher than other regions. But the SOC storage amount(per unit) was highest in Jeju island indicating that volcanic ashes might influence on the relatively higher SOC amount. Based on these results, the SOC storage value was shown as 8.4 trillion won in ROK. Even though our SOC model was not fully validated due to lacks of measured SOC data, our approach can be useful for policy-makers in reducing soil organic carbon emission from soils against climate changes.

• Proceedings of the Korea Water Resources Association Conference
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• 2016.05a
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• pp.308-308
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• 2016

기후변화는 하천 수문특성을 변화시켰으며, 이로 인해 홍수와 가뭄과 같은 자연재해로 인한 피해와 발생빈도가 점차 증가하고 있다. 이에 따라 기후변화에 대비한 유역관리를 위해 유역특성을 고려한 정확한 수문예측이 요구되고 있다. 다양한 유역특성 중에서 수문곡선의 감수부는 유역의 토성, 경사, 대수층 특성 등에 영향을 받는다. 하지만 대부분 유출 모의 모형에서는 이를 고려하지 않고 유역 전체가 동일한 감수특성을 가지고 있다고 가정하여 모의를 한다. 이에 본 연구에서는 미계측 유역에서의 유출 모의가 가능한 Soil and Water Assessement Tool(SWAT) 모형을 활용하여 소유역 별 수문곡선의 감수부 특성이 기저유출 모의 결과에 대한 영향을 분석하였다. alpha factor는 SWAT 모형의 다양한 매개변수 중 한가지로 수문곡선의 감수부 특성을 나타내는 매개변수이다. 이에 본 연구에서는 소유역 중에서 실측 유량자료가 활용 가능한 소유역에 대하여 Web-based RECESS를 통해 alpha factor를 직접 산정하였다. 이렇게 산정한 alpha factor를 SWAT 모형에 적용하여 유출 모의를 한 뒤 모의 결과로부터 기저유출을 분리하였다. SWAT 모형의 기본 입력값(default value)을 적용에 따른 기저유출 분리결과와 실측 유량자료로부터 산정된 alpha factor 적용에 따른 기저유출 분리결과를 비교한 결과 실측 유량자료로부터 산정된 alpha factor를 적용 시 기저유출 모의 결과가 실제 기저유출에 더 가까운 값을 나타내었다. 하지만 alpha factor의 산정 방법 특성 상 실측 유량자료 부재시 이를 산정할 수 없다는 한계점이 있다. 이에 실측 유량자료를 이용한 기존의 alpha factor 산정 방법을 대체할 수 있는 alpha factor 산정 방법을 개발한다면 정확한 기저유출 모의가 가능할 것으로 기대된다.

• Journal of Microbiology and Biotechnology
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• v.29 no.10
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• pp.1656-1664
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• 2019

Isoprene has the potential to replace some petroleum-based chemicals and can be produced through biological systems using renewable carbon sources. Ralstonia eutropha can produce value-added compounds, including intracellular polyhydroxyalkanoate (PHA) through fatty acid and lipid metabolism. In the present study, we engineered strains of R. eutropha H16 and examined the strains for isoprene production. We optimized codons of all the genes involved in isoprene synthesis by the mevalonate pathway and manipulated the promoter regions using pLac and pJ5 elements. Our results showed that isoprene productivity was higher using the J5 promoter ($1.9{\pm}0.24{\mu}g/l$) than when using the lac promoter ($1.5{\pm}0.2{\mu}g/l$). Additionally, the use of three J5 promoters was more efficient ($3.8{\pm}0.18{\mu}g/l$) for isoprene production than a one-promoter system, and could be scaled up to a 5-L batch-cultivation from a T-flask culture. Although the isoprene yield obtained in our study was insufficient to meet industrial demands, our study, for the first time, shows that R. eutropha can be modified for efficient isoprene production and lays the foundation for further optimization of the fermentation process.