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A Study on the Effects of Anticareinogenie Activity of Chondria Crassicaulis  

Jeon Kwang-Hye (Department of Food and Nutrition, Marine Biotechnology Center for Biofunctional Material Industries, Silla University)
Shin Mi-Ok (Department of Food and Nutrition, Marine Biotechnology Center for Biofunctional Material Industries, Silla University)
Bae Song-Ja (Department of Food and Nutrition, Marine Biotechnology Center for Biofunctional Material Industries, Silla University)
Publication Information
Journal of Nutrition and Health / v.38, no.7, 2005 , pp. 503-511 More about this Journal
Abstract
In this study we investigated the biological activity of Chondria Crassicaulis (CC) on the human cancer cells. CC was extracted with methanol and further fractionated into four different types: hexane (CCMH), methanol (CCMM), butanol (CCMB), and aqueous (CCMA) partition layers. We determined the cytotoxic effect of these layers on human cancer cells by MTT assay. Among various partition layers of CC, the CCMM and CCMB showed the strong cytotoxic effects at 150 ${\mu}g$/ml which resulted $98.91\%$, $92.96\%$ on HeLa cell lines and $95.47\%$, $77.05\%$ on MCF-7 cell lines. And, the anti-proliferative effect of CC was accompanied by a marked inhibition of cyclooxygenase (COX-2), Caspase-3 and IAP (cIAP-1, cIAP-2 and XIAP) protein and concomitant induction of p53, p21 and Survivin protein. However, CC did not affect the level of Bax, Bcl-2 and Bcl-XL- protein. Also, we observed quinone reductase (QR) induced effects in all fraction layers of CC on HepG2 cells. The QR induced effects of the CCMH and CCMM on HePG2 cells at 120 ${\mu}g$/mL concentration indicated 3.73 and 2.45 with the control value of 1.0. Although further studies are needed, the present work suggests that CC may be a chemopreventive agent for the treatment of human cancer cells.
Keywords
cytotoxicity; quinone reductase; Chondria crassicaulis (CC);
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1 Shim SM, Kim MH, Bae SJ. Cytotoxicity and quinone reductase induced effects of Daucus carota L. leaf extracts on human cancer cells. J Korean Soc Food Sci Nutr 30(1): 86-91, 2001
2 Park JH, Kang KC, Baek SB, Lee YH, Rhee KS. Separation of antioxidant compounds from edible marine algae. Koran J Food Sci Technol 23 (3) : 256-260, 1991
3 Alley MC, Scudiero DA, Monks A, Hursey ML, Czerwinski MJ, Fine DL, Abbortt BJ, Mayo JG, Shoemaker RH, Boyd MR. Feasibility of drug screening with panels of human tumor cell line using a microculture tetrazolium assay. Cancer Res 48: 589-601, 1988
4 Baliga BC, Kumar S. Role of Bcl-2 family of proteins in malignancy. Hematol Oncol 20 (2) : 63-74,,2002   DOI   ScienceOn
5 Bae SJ. Studies on the antioxidative and antimicrobial effects of Chondria crassicaulis. J Life Sci 14 (3) : 411-416, 2004   DOI
6 Gerh?user C, Klimo K, Heiss E, Neumann I, Gamal-Eldeen A, Knauft J, Liu GY, Sitthimonchai S, Frank N. Mechanism-based in vitro screening of potential cancer chemopreventive agents. Mutation Research 523-524: 163-172,2003   DOI   ScienceOn
7 Lee YP, Yoon SY. Taxonomy of Chondria (Rhodopfyta) in Korea Algae. Korean J Phycology 11 (1): 107-139, 1996
8 Reddy L, Odhav B, Bhoola K. Natural products for cancer prevention: a global perspective. Phannacology & Therapeutics 99: 1-13,2003   DOI   ScienceOn
9 Ryu BH, Kim DS, Cho KJ, Sin DB. Antitumor activity of seaweeds toward sarcoma-180. Koran J Food Sci Technol 21 (5): 595-600,1989
10 Femandes-Alnemri T, Litwack G, Alnemri ES. CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-l beta-converting enzyme. J Bioi Chem 269: 30761-30764,1994
11 Shon YH, Nam K. Antimutagenicity and induction of anticarcinogenic phase II enzymes by basidiomycetes. Journal of Ethnopha-macology 77: 103-109,2001
12 Stavric B. Role of chemopreventers in human diet. Clin Biochem 27: 319, 1994   DOI   PUBMED   ScienceOn
13 Carmichael J, De Graff WG, Gazder AF, Minna JD, Mitchell JB. Evalution of a tetrazolium based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res 47: 936-942, 1987
14 Steinkellner H, Rabot S, Freywald C, Nobis E, Scharf G, Chabicovsky M, Knasmiiller S, Kassie F. Effects of c rucifrous vegetable and their constituents on drug metabolizing enzymes involved in the bioactivation of DNA-reactive dietary carcinogens. Mutation Research 480-481: 285-297, 2001
15 Dunlap WC, YAmamoto Y. molecule antioxidants in marine organism: antioxidant activity of mycosporine-glycine. Comp Biochem Physiol 112B: 105-114,1995   DOI   ScienceOn
16 Bae SJ. The effects of anticarcinogenic activity of Solanum tuberosum peel fractions. J Korean Soc Food Sci Nutr 31 (5) : 905-909,2002
17 Prochaska HJ, Santamaria AB. Direct measurement of NAD(P) H: Quinone reductase from cells cultured in microtiter wells: A screening assay for anticarcinogenic enzyme in ducers. Anal Biochem 169: 328-336, 1988   DOI   ScienceOn
18 Smali A, Zhivotoxvsky B, Jones D, Nagata S, Orrenius S. Apoptpsis: cell death defined by caspase activation. Cell Death Differ 6: 495-496, 1999   DOI   ScienceOn
19 Edmonds JS, Shibata Y, Yang F, Morita M. Isolation and synthesis of I-deoxy-l-dimethylarsinoylribitol-5-sulfate, a natural constituent of Condria crassicaulis and other red algae. Tetrahedron Letters 38 (33): 5819-5820, 1997   DOI   ScienceOn
20 Talalay P, Benson AM. Elvation of quinone reductase activity by anticarcinogenic antioxidants. Advances in Enzyme Regulation 20: 287-300, 1982   DOI   ScienceOn
21 Shim SM, Choi SW, Bae SJ. Effets of Punica granatum L. Fraction on quinone reductase induction and growth inhibition on several cancer cells. J Korean Soc Food Sci Nutr 30(1): 8085,2001
22 Schwartsmann G,ha AB, Berlinck R, GS, Jimeno J. Marine organisms as a souce of new anticancer agents. Oncology 2: 221-225,2001   DOI   ScienceOn
23 Park YB, Kim IS, Yoo SJ, Ahn JK., Lee TG, Park DC, Kim SB. Elucidation of anti-tumor initiator and promoter derived from seaweed-2: investigation of seaweed extracts suppressing mutagenic activity of PhIP and MeIQx. J Korean Fish Soc 31(4): 581-586,1998
24 Lee JH, Lee E, Park J, Kim E, Kim J. In vivo p53 function is indispensable for DNA damage-induced apoptotic signaling in Drosophlia. FEBS Letters 550: 5-10,2003   DOI   PUBMED   ScienceOn
25 Wefers H, Komai T, Talalay P, Sies H. Protection against reactive oxygen species by NAD(P) H: quinone reductase induced by the dietary antioxidant butylated hydroxyanisole (BHA). Federation of European Biochemical Societies 169 (1): 63-66, 1984
26 Park YB, Kim IS, Yoo SJ, Ahn JK., Lee TG, Park DC, Kim SB. Elucidation of anti-tumor initiator and promoter derived from seaweed-3: anti-tumor promoters of Ecklonia stolonifera extracts. J Korean Fish Soc 31 (4): 587-593, 1998
27 Lee HM, Kim YJ, Park TS. Induction of Apoptoisis by (-)epigallocatechin- 3-gallate in HL-6O Cells. J Korean Soc Food Sci Nutr 36 (4) : 382-388,2003
28 Doll R. Peto R. The cause of cancer. Quantitative estimates of available risks of cancer in the United States today. J Natl Cancer Inst 66: 1192-1308, 1981
29 Yu J, Leung WK, Ebert MP, et aI. Increased expression of survivin in gastric cancer patients and in first degree relative. Br J Cancer 87: 91-97, 2002   DOI   ScienceOn
30 Krinsky Nl. Effects of carotenoids in celluar and animal systems. Am J Clin Nutr 53: 38S-246S, 1991
31 Min SH, Shon YH, Park CH, Lee WS, Joo YE, Choi SK, Rew JS, Park CS, Kim SJ. Expression of cyclooxygenase-2 and p53 in gastric cancer and precursor lesions. Korean J Gastroenterology 41(4):268-276,2003
32 Subbaramaiah K, Altorki N, Chung WJ, Mestre JR, Sampat A, Dannenberg AJ. Inhibition of cyclooxygenase-2 gene expression by p53. J Bioi Chem 274: 10911-10915, 1999   DOI   ScienceOn
33 Prochaska HJ. Screening strtegies for the detection of anticarcinogenic enzyme inducers. J Nutr Biochem 5: 360, 1994   DOI   ScienceOn