• BMB Reports
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• 제28권4호
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• pp.336-341
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• 1995

Using a mutant M13 phage derivative lacking a great part of the complementary strand synthesis origin, we identified six single-strand initiation (ssi) signals for DNA replication in pACYC184, pLG214, pGKV21, and pDPT270 plasmids, and named them $ssiA_{YC}$, $ssiA_{LG}$, $ssiB_{LG}$, $ssiA_{KV}$, $ssiA_{PT}$, and $ssiB_{PT}$, respectively. Two of them were from pDPT270, one from downstream the on of pACYC184, two from pLG214, one from upstream the plus origin of pGKV21. Introduction of these ssi signals into the deleted $ori_c$ site of a mutant filamentous M13 phage ($M13{\Delta}lac182$) resulted in the restoration of growth activity of this phage. These ssi signals were classified into a number of groups on the basis of sequence similarity. $ssiA_{YC}$ and $ssiA_{LG}$ show extensive sequence homology to the n'-site (primosome assembly sites) of ColE1, whereas $ssiB_{PT}$ is homologous to the n'-site of ${\Phi}X174$. $ssiA_{PT}$ belongs to G4-type ssi signals which require only dnaG primase and SSB protein for the priming of replication. In addition, possible biological roles of these ssi signals are discussed.

• 한국생물정보학회:학술대회논문집
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• 한국생물정보시스템생물학회 2005년도 BIOINFO 2005
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• pp.139-143
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• 2005

Cytochrome P450 14${\alpha}$-sterol demethylase enzyme (CYP51) is the target a of azole type antifungals. The azole blocks the ergosterol synthesis and thereby inhibits fungal growth. A three-dimensional (3D) homology model of CYP51 from Candida albicans was constructed based on the X-ray crystal structure of CYP51 from Mycobacterium tuberculosis. Using this model, the binding modes for the substrate (24-methylene-24, 25-dihydrolanosterol) and the known inhibitors (fluconazole, voriconazole, oxiconazole, miconazole) were predicted from docking. Virtual screening was performed employing Structure Based Focusing (SBF). In this procedure, the pharmacophore models for database search were generated from the protein-ligands interactions each other. The initial structure-based virtual screening selected 15 compounds from a commercial available 3D database of approximately 50,000 molecule library, Being evaluated by a cell-based assay, 5 compounds were further identified as the potent inhibitors of Candida albicans CYP51 (CACYP51) with low minimal inhibitory concentration (MIC) range. BMD-09-01${\sim}$BMD-09-04 MIC range was 0.5 ${\mu}$g/ml and BMD-09-05 was 1 ${\mu}$g/ml. These new inhibitors provide a basis for some non-azole antifungal rational design of new, and more efficacious antifungal agents.

• Biomolecules & Therapeutics
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• 제3권1호
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• pp.72-79
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• 1995

The antiulcer effects of newly synthesized prostaglandin derivatives were investigated in various experimental ulcer models and on gastric secretion in rats. HK-3 and HK-4, PG $E_2$derivatives, prevented the formation of acute gastric ulcer induced by ethanol or aspirin in pylorus-ligated rats. The ulcer formation was moderately inhibited by HK-1 and HK-2, PG $F_{2{\alpha}}$ derivatives, and aggravated by SK-1, SK-2 and SK-3, PG $F_{2{\alpha}}$ derivatives. HK-3 and HK-4 reduced the volume, acid output and pepsin output of gastric juice in pylorus-ligated rats. The gastric perfusion with physiologic saline(pH 6.0) showed relatively constant acid secretion and indomethacin increased the acid secretion. The acid secretion was markedly decreased by PG $E_2$but PG $F_{2{\alpha}}$ caused little change. Prostaglandin derivatives, especially HK-3 arid HK-4, significantly inhibited the acid secretion induced by indomethacin. The results show that, PG $E_2$ derivatives, HK-3 and HK-4, inhibit acid secretion and also have protective effects on gastric ulceration induced by ethanol or aspirin.

• 미생물학회지
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• 제47권1호
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• pp.30-37
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• 2011

조류 인플루엔자 바이러스(AIV) 아형을 ultra-time PCR법(UPCR)을 이용하여 초스피드로 진단할 수 있는 방법을 고안하였다. 표적 대상의 프라이머는 AIV H5N1 아형의 hemagglutinin(HA) 유전자 중 가장 상보성이 높은 133 bp의 부위를 선택하였고, 실험의 안전을 위하여 인공합성의 방법으로 제작하였다. 압타머와 결합한 molecular beacon 기반 Mini-Opticon Q-PCR 기기를 사용한 UPCR법으로, 총 UPCR 반응액의 양을 10 ${\mu}l$으로, UPCR과 용융온도 분석시간을 15분 이내로 매우 짧게 단축시켰다. 민감도 측정에서 최소의 주형인 5분자의 HA 유전자만으로 정확히 AIV의 특이적 133 bp를 합성하였다. UPCR로 디자인된 이 PCR은 AIV 아형의 진단에 적용될 수 있을 뿐 아니라, UPCR이 기반되는 진단을 이용하여 다른 병원체에도 널리 적용 될 수 있을 것으로 기대된다.

• Bulletin of the Korean Chemical Society
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• 제32권6호
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• pp.2015-2020
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• 2011

A series of isoxazol-indolin-2-one was designed for GSK-3${\beta}$ inhibitors as novel anticancer agents based on their binding mode analysis in GSK-3${\beta}$ crystal structure. Total 21 compounds were synthesized and evaluated for their inhibitory activity against two tumor cell lines (DU145 and HT29). Most of the synthesized compounds were potent with above 80% inhibitory activity at 100 ${\mu}M$, and several compounds were examined for inhibitory activity against GSK-3${\beta}$. Among them, 15(Z) ($R_1$=H, $R_2$=3-Cl-phenyl) was most active with 78% inhibition of tumor cell line (HT29) at 20 ${\mu}M$ and 72% inhibition of GSK-3${\beta}$ at 20 ${\mu}M$.

• 한국염색가공학회:학술대회논문집
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• 한국염색가공학회 2011년도 제44차 학술발표회
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• pp.36-36
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• 2011

We synthesized new dye sensor based on indole compound. Through the UV-vis absorptions, we analyzed chemosensing properties to explain metal binding properties. The peak absorptions increased at 472 nm when added metal cations($Cd^{2+}$, $Cu^{2+}$, $Hg^{2+}$, $Fe^{2+}$, $Zn^{2+}$, $Ni^{2+}$ and $Cr^{3+}$) and gradually decreased the peak at 516 nm. Thus, this UV-Vis absorption behavior clearly showed the metal binding reaction. To measure energy level of used dye sensor, HOMO/LUMO energy value was calculated with cyclovaltagramm(CV) and using computational calculation method, in which we estimated the optimum structure of dye sensor. CV and computational calculation method, both compared to find suitable geometric structure. (with almost same energy values.) From the computational calculation, dye sensor has plane structure. So, Amine and ketone in the dye sensor faced each other and makes position to bind metal cations. In addition, these positions was supported pull-push electron system and generated MLCT process, when the dye sensor was bonded with the metal cations and resulted chemosensing properties. Through the electrochemical and computational calculation method analyze, we proposed the chemosensing principles that the dye sensor bind the metal cation between ketone and amine. Finally, the formation type of metal ion bindings was determined by Job's plot measurements.

• BMB Reports
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• 제31권4호
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• pp.355-363
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• 1998

MHC unrestricted, antigen nonspecific killing by $CD4^+$ T-cells against virally-infected target cells was induced following cross-linking of CD4 molecules. The cytotoxicity of antibody-activated $CD4^+$ T-cells was abolished by genistein (4',5,7-trihydroxyisoflavone), a protein tyrosine kinase (PTK) inhibitor, but not by H-7, a protein kinase C (PKC) inhibitor. Genisteintreated human or bovine peripheral blood $CD4^+$ T-cells lacked PTK activity and failed to kill virally-infected target cells even after cross-linking of CD4 molecules. The cross-linking of CD4 molecules did not induce effector cell proliferation or the transcription of TNF ${\beta}$. TNF ${\beta}$ synthesis was up-regulated by incubating antibody activated effector cells with bovine herpesvirus type 1 (BHV-1) infected D17 target cells. Anti-TNF ${\beta}$ antibody partially abrogated direct effector cell-mediated antiviral cytotoxicity. On the other hand, this antibody effectively neutralized antiviral activity of effector and target cell culture supernatants against BHV-1 infected D17 cells. The inhibition level of the antiviral activity by the antibody was dependent on effector and target cell ratio. These findings have importance to define the mechanisms of how CD4 cytotoxic cells control viral infection.

• Journal of the Korean Wood Science and Technology
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• 제48권2호
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• pp.166-180
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• 2020

Rhododendron schlippenbachii have been used as a medicine because of their various biological activities. In this study, R. schlippenbachii ethanol extract was evaluated for the treatment of vitiligo. The R. schlippenbachii ethanol extract did not show any cell cytotoxicity. The effect on mushroom tyrosinase and cellular tyrosinase activities were further assessed. In addition, the determination of melanin content in melanocytes was measured using both the B16 melanoma cells and C57BL/6J Ler-vit/vit mice. Finally, the existence of quercetin in R. schlippenbachii was confirmed by qualitative analysis using HPLC. The results clearly demonstrated the R. schlippenbachii extract enhanced melanogenesis and also increased tyrosinase activity in cultured melanoma cells and C57BL/6J Ler-vit/vit mice. In addition, treatment with R. schlippenbachii extract led to a higher content of melanin and eumelanin in C57BL/6J Ler-vit/vit mice hair than in control (untreated) mice, which demonstrated the therapeutic effect of hair-graying associated with vitiligo. Finally, we confirmed a notable increase in melanocytes in the skin of C57BL/6J Ler-vit/vit mice treated with R. schlippenbachii extract compared with the control. Extracts of R. schlippenbachii was shown to be potent tyrosinase and melanin synthesis activator in B16 melanoma cells. The R. schlippenbachii extract have significantly higher melanin content than the untreated control in C57BL/6J Ler-vit/vit mice hair. The results suggest that R. schlippenbachii extract might be considered as an alternative treatment for improvement of vitiligo.

• 한국방재학회 논문집
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• 제10권1호
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• pp.81-88
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• 2010

수자원의 관리 및 계획시 강우, 유출, 유량과 같이 다양한 종류에 의한 수문사상의 합성 및 분석이 요구된다. 다양한 수문사상들은 대부분 추계학적모형에 의한 해석이 필요하며, 이중 적절한 시계열모의결과를 나타낼 수 있는 자기회귀모형 적용을 시도하였다. 본 연구에서는 낙동강 상류에 위치한 안동댐과 임하댐 두 관측소의 월유출량 자료를 이용하여 최적의 자기회귀모형을 검토하였으며, 분석결과 AR(3) 모형의 매개변수($\phi_1$, $\phi_2$, and $\phi_3$)가 가장 적합한 것으로 나타났으며, 다양한 분석 및 평가결과 AR(3)모형이 효과적이고 정확한 것으로 나타났다.

• 한국미생물·생명공학회지
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• 제35권1호
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• pp.36-40
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• 2007

Redox signaling은 단백질을 산화환원 시키는 세포의 중요 신호가 전달되어, 그 단백질의 기능이 변화함으로써 세포의 성장 및 사멸을 조절하게 되는 과정이다. 단백질 합성 구성원의 산화, 환원 과정에 의한 단백질 합성 조절을 알아보기 위해 환원제인 DTT 존재 하에 단백질 합성 활성을 관찰한 결과 DTT가 존재하지 않는 것에 비해 단백질합성이 1.4배 정도 증가됨이 관찰되어 redox potential을 보이는 것으로 보아 환원제가 단백질 합성을 좀 더 증진시키는 것으로 사료된다. DTT에 의한 이러한 현상은 산화환원 조절 단백질인 thioredoxin를 첨가한다면 thiol기에 환원력이 전달되어 단백질합성이 더욱 촉진되기 때문에 효모에서 thioredoxin유전자를 cloning하고 이로부터 효모에서 GST-thioredoxin을 분리하였다. DTT 존재 하에 산화환원 조절 단백질인 thioredoxin을 농도별로 첨가하였을 때의 단백질 합성이 어떻게 조절되는지 알아보았다. 반응 액에 DTT를 넣은 것과 넣지 않은 것을 사용하여 thioredoxin을 0ng, 18ng, 90ng, 460ng, 2,300 ng의 농도로 각각 넣어서 반응시켜 보았다. 이렇게 반응시킨 반응물에서 만들어진 단백질 활성을 측정하였는데 thioredoxin의 농도가 높아질수록 그 활성이 높게 나타났으며, thioredoxin을 넣은 것이 넣지 않은 것에 비해 활성이 약 4배 이상 높게 나왔다 이 결과는 산화환원 조절 단백질인 thioredoxin이 환원력을 단백질합성구성원에 효율적으로 전달하는데 관여함을 보여주는 것이며, 산화환원이 단백질 합성 시 중요한 신호전달 과정임을 암시한다.