• Title/Summary/Keyword: biological stability

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Community changes in carabid beetles (Coleoptera: Carabidae) through ecological succession in abandoned paddy fields

  • Do, Yu-No;Jeong, Kwang-Seuk;Lineman, Maurice;Kim, Ji-Yoon;Kim, Hang-Ah;Joo, Gea-Jae
    • Journal of Ecology and Environment
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    • v.34 no.3
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    • pp.269-278
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    • 2011
  • Carabid beetle community changes in paddy fields, abandoned paddy fields, and mountains were investigated to understand differences in species diversity and composition of different habitat types in small agricultural landscapes in South Korea. A total of 2,938 individuals from 37 species were identified and classified from the studied habitats. Diversity in the mountains was higher than that in abandoned paddy fields. As the number of years since abandonment increased, the diversity and stability of the carabid beetle community also increased. Abandoned paddy fields in some paddy field areas and adjoining mountainous areas, left with preconditions for management and control, have provided the opportunity to improve the diversity and habitat of small agricultural landscapes. Species composition changed continuously from the paddy fields to the mountains, in accordance with individual species habitat preferences. Abandoned paddy fields in small agricultural landscapes are believed to play an important role as transitional buffer zones between paddy fields and the adjacent mountainous areas.

PREPARATION OF POLYSTYRENE BEADS CONTAINING SULFONAMIDE GROUPS AND THEIR APPLICATION TO POLYMERIC BIOCIDES

  • Kim, Cheol-Jin;Kim, Jee-Yeon;Byun, Jang-Woong;Kim, Jae-Eun;Lee, Yoon-Sik;Yoon, Je-Yong
    • Environmental Engineering Research
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    • v.11 no.6
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    • pp.325-332
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    • 2006
  • A novel series of polystyrene (PS) beads containing various sulfonamide groups was prepared, and their chemical stabilities in an aqueous solution were tested in order to determine their ability to inactivate microbes. By reacting aminomethyl polystyrene (AM PS) beads or carboxy polystyrene beads with various benzenesulfonic acid derivatives, the sulfonamide groups were introduced on the PS beads. The characteristics of the product beads were analyzed by elementary analysis after the substitution of various sulfonamide groups. Energy Dispersive Spectroscopy (EDS), and FT-IR analysis were used to analyze the elemental functional group composition, respectively. The hydrolytic stabilities of the PS beads containing various sulfonamide groups along with the relationship between the swelling ratio and their hydrophilicity were investigated. The antibacterial activity of the beads was determined by their ability to inactivate E. coli. This study reports that PS beads containing sulfonamide groups had lasting antibacterial efficacy over a satisfactory period, whilst maintaining their chemical stabilities against hydrolysis. The 8 synthesized polymer beads exhibited antibacterial ability.

Stability of Partial Nitrification and Microbial Population Dynamics in a Bioaugmented Membrane Bioreactor

  • Zhang, Yunxia;Xu, Yanli;Jia, Ming;Zhou, Jiti;Yuan, Shouzhi;Zhang, Jinsong;Zhang, Zhen-Peng
    • Journal of Microbiology and Biotechnology
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    • v.19 no.12
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    • pp.1656-1664
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    • 2009
  • Bioaugmentation of bioreactors focuses on the removal of numerous organics, with little attention typically paid to the maintenance of high and stable nitrite accumulation in partial nitrification. In this study, a bioaugmented membrane bioreactor (MBR) inoculated with enriched ammonia-oxidizing bacteria (AOB) was developed, and the effects of dissolved oxygen (DO) and temperature on the stability of partial nitrification and microbial community structure, in particular on the nitrifying community, were evaluated. The results showed that DO and temperature played the most important roles in the stability of partial nitrification in the bioaugmented MBR. The optimal operation conditions were found at 2-3 mgDO/l and $30^{\circ}C$, achieving 95% ammonia oxidization efficiency and nitrite ratio ($NO_2^-/{NO_x}^-$) of 0.95. High DO (5-6 mg/l) and low temperature ($20^{\circ}C$) had negative impacts on nitrite accumulation, leading to nitrite ratio drop to 0.6. However, the nitrite ratio achieved in the bioaugmented MBR was higher than that in most previous literatures. Denaturing gradient gel electrophoresis (DGGE) and fluorescence in situ hybridization (FISH) were used to provide an insight into the microbial community. It showed that Nitrosomonas-like species as the only detected AOB remained predominant in the bioaugmented MBR all the time, and coexisted with numerous heterotrophic bacteria. The heterotrophic bacteria responsible for mineralizing soluble microbial products (SMP) produced by nitrifiers belonged to the Cytophaga-Flavobacterium-Bacteroides (CFB) group, and $\alpha$-, $\beta$-, and $\gamma$- Proteobacteria. The fraction of AOB ranging from 77% to 54% was much higher than that of nitrite-oxidizing bacteria (0.4-0.9%), which might be the primary cause for the high and stable nitrite accumulation in the bioaugmented MBR.

Dictyostelium discoideum Ax2 as an Assay System for Screening of Pharmacological Chaperones for Phenylketonuria Mutations

  • Kim, Yu-Min;Yang, Yun Gyeong;Kim, Hye-Lim;Park, Young Shik
    • Journal of Microbiology and Biotechnology
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    • v.25 no.6
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    • pp.782-787
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    • 2015
  • In this study, we developed an assay system for missense mutations in human phenylalanine hydroxylases (hPAHs). To demonstrate the reliability of the system, eight mutant proteins (F39L, K42I, L48S, I65T, R252Q, L255V, S349L, and R408W) were expressed in a mutant strain (pah-) of Dictyostelium discoideum Ax2 disrupted in the indigenous gene encoding PAH. The transformed pah - cells grown in FM minimal medium were measured for growth rate and PAH activity to reveal a positive correlation between them. The protein level of hPAH was also determined by western blotting to show the impact of each mutation on protein stability and catalytic activity. The result was highly compatible with the previous ones obtained from other expression systems, suggesting that Dictyostelium is a dependable alternative to other expression systems. Furthermore, we found that both the protein level and activity of S349L and R408W, which were impaired severely in protein stability, were rescued in HL5 nutrient medium. Although the responsible component(s) remains unidentified, this unexpected finding showed an important advantage of our expression system for studying unstable proteins. As an economic and stable cell-based expression system, our development will contribute to mass-screening of pharmacological chaperones for missense PAH mutations as well as to the in-depth characterization of individual mutations.

Evaluation of Ecosystem Changes in Upo Wetland through Analysis of Benthic Macroinvertebrate Community Data (저서성 대형무척추동물 군집자료분석에 의한 우포늪의 생태계변화 평가)

  • Tae-Won Hwang;Seong-Woo Bae;Chun-Sik Yoon;Sung-Jin Hong;Seon-Woo Cheong
    • Journal of Environmental Science International
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    • v.32 no.2
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    • pp.99-120
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    • 2023
  • The community structure of benthic macroinvertebrates in Upo wetland was identified, and the biological water quality was evaluated. In addition, through statistical analysis of current and literature data, ecological changes over time were evaluated for each wetland. Benthos were quantitatively collected in March, June, and September of 2020 and 2021, and 4 phyla, 5 classes, 16 orders, 42 families, 81 species and 3,406 individuals were identified. In the functional feeding group of Upo wetland, predators were dominant with 34 species (45.95%) and 1,504 individuals (41.84%). In the habitual dwelling group, sprawlers and swimmers showed the highest proportion in the number of species and individuals. Average biological indices in Mokpo and Upo were the highest and lowest, respectively, and it is considered that Mokpo maintains the healthy ecosystem for benthic macroinvertebrates. Community stability was high in Upo, and other wetlands are thought to be stabilizing. The ecological score of benthic macroinvertebrate community is considered to be more suitable index among three biological water quality evaluation indices for the environmental evaluation of Upo wetland. The evaluation results on changes in environmental quality showed that Upo has stable ecosystem without significant change, Mokpo and Sajipo have significant increases in some indices.

Effects of oil refining processes on oil characteristics and oxidation stability of sesame oil (정제공정이 참기름의 유지특성과 산화안정성에 미치는 영향)

  • Han, Jin-Suk;Ahn, Seung-Yo
    • Applied Biological Chemistry
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    • v.36 no.4
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    • pp.284-289
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    • 1993
  • The effect of oil refining processes-degumming, alkali-refining, bleaching and deodorizing-on oil characteristics and oxidation stability of sesame oil were investigated. The colors(L, a, b) of samples were markedly changed and their peroxide and acid values were decreased, while the other characteristics of samples were not changed during refining stages. The L values of alkali-refined, bleached and deodorized sesame oils were largely decreased and their a values were increased due to browning reaction during the storage at $70^{\circ}C$. The colors of crude and degmmed sesame oils were very stable and their peroxide, free fatty acid and conjugated dienoic acid values were slowly increased. Volatile carbonyl compounds formed by oxidation were increased during the storage at $70^{\circ}C$. The results indicated that refining processes did not affect the sesame oil characteristics but decreased the oxidation stability of sesame oil.

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Processing and quality stability of precooked frozen fish foods : (II) Quality stability of sardine burger (조리냉동식품의 가공 및 저장 중 품질안정성 : (II) 정어리버어거의 동결저장 안정성)

  • Ihm, Chi-Won;Kim, Jin-Soo;Joo, Dong-Sik;Lee, Eung-Ho
    • Applied Biological Chemistry
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    • v.35 no.4
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    • pp.260-264
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    • 1992
  • In present paper, we investigated the quality stability of sardine burgers during storage at $-20{\pm}2^{\circ}C$. During frozen storage of sardine burger, the PH were decreased, while volatile basic nitrogen contents were increased. The results of changes in peroxide values, thiobarbituric acid values, fatty acid compositions and color values during frozen storage showed that lipid oxidation and discolorization of antioxidant treated sardine burger and vacuum packed sardine burger could be effectively retarded. The changes in the taste compounds such as free amino acid, nucleotide and their related compounds, total creatinine, betaine and trimethylamine oxide, total amino acids and texture profile analysis of vacuum packed sardine burger were negligible during frozen storage. From the results of sensory evaluation and chemical experiments, the vacuum packed sardine burger could be preserved in good quality during frozen storage of 90 days.

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Sonoelectrodeposition of RuO2 electrodes for high chlorine evolution efficiencies (초음파 전기증착법을 활용한 고효율 염소 발생용 루테늄 옥사이드 전극)

  • Luu, Tran Le;Kim, Choonsoo;Yoon, Jeyong
    • Journal of Korean Society of Water and Wastewater
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    • v.31 no.5
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    • pp.397-407
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    • 2017
  • A dimensionally stable anode based on the $RuO_2$ electrocatalyst is an important electrode for generating chlorine. The $RuO_2$ is well-known as an electrode material with high electrocatalytic performance and stability. In this study, sonoelectrodeposition is proposed to synthesize the $RuO_2$ electrodes. The electrode obtained by this novel process shows better electrocatalytic properties and stability for generating chlorine compared to the conventional one. The high roughness and outer surface area of the $RuO_2$ electrode from a new fabrication process leads to increase in the chlorine generation rate. This enhanced performance is attributed to the accelerated mass transport rate of the chloride ions from electrolyte to electrode surface. In addition, the electrode with sonodeposition method showed higher stability than the conventional one, which might be explained by the mass coverage enhancement. The effect of sonodeposition time was also investigated, and the electrode with longer deposition time showed higher electrocatalytic performance and stability.

How can improve the insufficient success rate at immediate loading? (임상가를 위한 특집 1 - Immediate loading 부족한 성공률 5% 채우기)

  • Jun, Sang-Ho;Ahn, Jin-Soo;Ryu, Jae-Jun;Kwon, Jong-Jin
    • The Journal of the Korean dental association
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    • v.51 no.4
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    • pp.190-197
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    • 2013
  • A titanium based screw shaped dental implant was first introduced by Branemark and a treatment protocol where the restoration of edentulous area by connecting abutment after the osseointegration of the titanium surface of the implant and surrounding bone structure has been proposed. Although this protocol is widely accepted as a standard up to date, the healing duration of 3-6 months as well as the need for provisional prostheses during this period present as a major drawback. Immediate loading has been accomplished through the advent of various implant designs, enforced surface treatments, diverse forms of abutment, and delicate surgical techniques together with the increase in demand from the patients. The success rate of the immediate loading technique has been first reported as 85.7% by Dr. Schnitman in 1990 which recently has been reported up to 100% in the case of immediate loading in single tooth by Dr. Kan. To ameliorate the success rate of immediate loading technique, selection of patients presenting a sound bone quality and quantity, acquiring primary stability through delicate surgical techniques and fabrication of prostheses which accounts for biological stabilities should all be taken into consideration. This presentation introduces the understanding of biological stability of immediate loading, various methods for measurement of stability and clinical cases regarding immediate loading technique.

A Study on the High Content Ceramide Stabilization Formulation with Cyclodextrin (사이클로덱스트린을 함유한 고함량 세라마이드 안정화 제형 연구)

  • Ye Ji Kim;Sang Woo Han;So Min Lee;Byungsun Cha;Hyojin Heo;Sofia Brito;Lei Lei;Sang Hun Lee;You-Yeon Chun;Ha Hyeon Jo;Hyung Mook Kim;Byeong-Mun Kwak;Bum-Ho Bin
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.49 no.2
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    • pp.97-106
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    • 2023
  • In this study, we would like to study the stabilization of the high content of ceramide formulation by containing cyclodextrin. Ceramide, which constitutes the intercellular lipid, a human skin barrier, is a very important ingredient in moisturizing maintenance by protecting moisture in the skin and strengthening the skin barrier. However, since ceramide is poorly soluble, even if it is included in the cosmetic formulation, it has a problem that it is slowly gelled or crystallized and deposited over time, making it difficult to containing a high amount of ceramide. Cyclodextrin is a cyclic oligosaccharide connected with glucose molecules and has a cylindrical structure with hydrophilic outer surface and hydrophobic inner surface, which is known to improve the physicochemical properties of drugs such as improving solubility and absorption of poorly soluble drugs. We demonstrated the stability of the formulation containing high amount of ceramide by measuring hardness and observing emulsion drops with polarized microscope. This study also demonstrated that the high-content ceramide formulation containing cyclodextrin has the effect of preventing gelation or crystallization of ceramide, thus having excellent environmental conditions stability and skin moisturization.