• Applied Biological Chemistry
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• v.41 no.1
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• pp.13-17
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• 1998

To improve extraction of insoluble proteins and functional properties of abolished proteins by protease. It was found that the optimum pH, optimum temperature, optimum treatment time and optimum unit of enzyme far extraction of protein were pH 9.0, $60^{\circ}C$, 8 hrs, 40 units. The foaming capacity and foaming satbility of sesame meal protein after treatment of enzyme were especially higher than control. The emulsion capacity and emulsion satbility of sesame meal protein were higher than control. Coil absorption as well as water absorption capacities of sesame meal protein were higher than control.

• Korean Journal of Ichthyology
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• v.30 no.3
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• pp.161-166
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• 2018

The olfactory organ of Korean endemic fish Microphysogobio yaluensis are described anatomically and histologically, focused on relationship to its habitat and ecology. The paired olfactory organs are located at the dorsal snout, and externally consist of two semicircular nostrils and single nasal flap. They internally have rosette structure with 22 to 24 units of lamellae and the raphe inside the olfactory chamber. The lamella is made up of the sensory and the non-sensory epitheliums. The sensory epithelium has olfactory receptor neurons, supporting cells and basal cells whereas the nonsensory epithelium has stratified epithelial cells, ciliated non-sensory cells and mucous cells with acidic and neutral mucins. These structures might be considered that M. yaluensis has the olfactory organ which corresponds to the high sensitivity for its habitat and ecology, and is usable as a taxonomic key.

• Journal of Korean Biological Nursing Science
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• v.26 no.1
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• pp.16-25
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• 2024

Purpose: This study aimed to investigate the effect of a video-based enteral nutrition education program using QR codes on the perception, knowledge, and performance of enteral nutrition for intensive care unit (ICU) nurses. Methods: This was a quasi-experimental study with a nonequivalent control group pre- and post-test design. The participants were nurses working at six ICUs within a single university hospital, with 55 nurses in the experimental group and 55 nurses in the control group. The video-based enteral nutrition education program using QR codes was repeated three times to the experimental group. Results: There was a statistically significant pre-post difference in perceptions between the two groups (Z = -4.04, p < .001) with 2.00 points (± 5.57) for the control group and 7.89 points (± 7.95) for the experimental group, in knowledge (Z = -7.48, p < .001) with 0.02 points (± 1.91) for the control and 4.18 points (± 2.33) for the experimental, and in performance (Z = -2.20, p = .028) with 0.06 points (± 3.96) for the control and 2.00 points (± 5.14) for the experimental. Conclusion: The video-based enteral nutrition education program using QR codes was effective in improving the perceptions, knowledge, and performance of enteral nutrition among ICU nurses. This enteral nutrition education program using QR codes in clinical education can contribute to evidence-based nursing practice by improving perceptions and knowledge of enteral nutrition.

• Journal of Korean Biological Nursing Science
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• v.11 no.1
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• pp.92-98
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• 2009

Purpose: This study was aimed to investigate the colony counts by different disinfecting processes and duration of the operation for humidifiers in an intensive care unit (ICU) in Busan. Method: The four steps of disinfecting process were taken with four different humidifiers according to the use of disinfectant (1:100 $Deconex^{(R)}$ 50FF/nothing) and refilling water (tap water/distilled water). First, the colony counts using water sample from wick was conducted at 0 hr, 24 hr, and 48 hr respectively after turning on the humidifiers. Next, the colony counts using waterdrop from the outlet was done at 0 hr, 24 hr, 48 hr, and 72 hr respectively after turning on the humidifiers. Result: No colony was counted after disinfecting with 1:100 $Deconex^{(R)}$ 50FF until 72 hr after turning on the humidifiers. However, without disinfecting with $Deconex^{(R)}$ 50FF, the colony count was increased in the humidifiers from 24 hr after turning on the humidifiers. The result was the same regardless of the refilling water. Conclusion: According to the results, the study found that disinfection of humidifiers in the ICUs with 1:100 $Deconex^{(R)}$ 50FF every 72 hr is effective to remove general bacteria.

• Applied Biological Chemistry
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• v.36 no.3
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• pp.191-196
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• 1993

This method using the synthesis substrate of $5-bromo-4-chloro-3-indolyl-{\alpha}-galactoside\;(X-{\alpha}-Gal)$ was examined for the differential enumeration of Bifidobacteria and lactic acid-producing bacteria. Bifidobacteria possess a high level of ${\alpha}-galactosidase$ activity. Bifidobacterium longum KCTC 3215 exhibited the highest ${\alpha}-galactosidase$ specific activity (8.57 units/mg protein). Determination of ${\alpha}-galactosidase$ activity using the PNPG procedure showed that Lactobacillus, Streptococcus, Pediococcus, and Leuconostoc strain had lower ${\alpha}-galactosidase$ activity as compared to Bifidobacteria. The $X-{\alpha}-Gal$ based medium is useful to identify Bifidobacteria among lactic acid-producing bacteria since the enzyme action of ${\alpha}-galactosidase$ spills $X-{\alpha}-Gal$ substrate and releases indol which impacts a blue color to Bifidobacterial colonies on agar plates. All strains of Bifidobacteria appeared as blue colonies on MRS agar medium supplemented with $100\;{\mu}M\;X-{\alpha}-Gal$ while colonies of other lactic acid-producing bacteria appeared white or light blue.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 1997.05a
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• pp.8-11
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• 1997

Current methods of evaluating soil contamination by heavy metals rely on analyzing samples for total contents of metals or quantities recovered in various chemical extracting solutions. Results from these approaches provide only an index for evaluation because these methodologies yield values not directly related to bioavailability of soil-borne metals. In addition, even though concentrations of metals may be less than those required to cause toxic effects to biota, they may cause substantial effects on soil chemical parameters that determine soil quality and sustainable productivity. The objective of this research was to characterize effects of Cu or Cd additions on soil solution chemistry of soil quality indices, such as pH, EC, nutrient cation distribution and quantity/intensity relations (buffer capacity). Metals were added at rates ranging from 0 to 400 mg/kg of soil. Soil solution was sequentially extracted from saturated pastes using vacuum. Concentrations of Cu or Cd remaining in soil solutions were very low as compared to those added to the soils, warranting that most of the added metals were recovered as nonavailable (strongly adsorbed) fractions. Adsorption of the added metals released cations into soil solution causing increases of soluble cation contents and thus ionic strength of soil solution. At metal additions of 200~400 mg/kg, EC of soil solution increased to as much as 2~4 dS/m; salinity levels considered high enough to cause detrimental effects on plant production. More divalent cations (Ca+Mg) than monovalent cations (K+Na) were exchanged by Cu or Cd adsorption. The loss of exchangeable nutrient cations decreased long-term nutrient supplying capacity or each soil. At 100 mg/kg or metal loading, the buffering capacity was decreased by 60%. pH of soil solution decreased linearly with increasing metal loading rates, with a decrement of up to 1.3 units at 400 mg Cu/kg addition. Influences of Cu on each of these soil quality parameters were consistently greater than those of Cd. These effects were of a detrimental nature and large enough in most cases to significantly impact soil productivity. It is clear that new protocols are needed for evaluating potential effects of heavy metal loading of soils.

• Journal of Mushroom
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• v.17 no.4
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• pp.241-246
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• 2019

Samples (10 mg/mL) of wild Grifola frondosa aqueous extract and solvent fractions were examined for fibrinolytic, thrombin inhibitory, acetylcholinesterase inhibitory, and antioxidative activities to determine the biological activities. The fibrinolytic activity of the aqueous extract and solvent fractions was 0.93 and 0.73 plasmin units/mL, respectively. The thrombin inhibitory activity of the butanol extract was 79.60%. The chloroform fraction had high acetylcholinesterase inhibitory activity (85.88%). The aqueous extract had low antioxidative activity (39.81%). The aqueous fraction hydrolyzed Bβ subunits of human fibrinogen but did not show any reactivity for the γ form of the human fibrinogen. The findings indicate the potential of wild Grifola frondosa for the development of drugs and bio-functional foods to prevent cardiovascular diseases.

• Journal of Applied Biological Chemistry
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• v.59 no.4
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• pp.299-304
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• 2016

When used for amplicon sequencing, Illumina platforms produce more than hundreds of sequence artefacts, which affects operational taxonomic units based analyses such as differential abundance and network analyses. Nevertheless it has become a major tool for fecal microbial community analysis. In addition, results from sequence-based fecal microbial community analysis vary depending on conditions of samples (i.e., freshness, time of storage and quantity). We investigated if freeze-drying samples could improve quality of sequence data. Our results showed reduced number of possible artefacts while maintaining overall microbial community structure. Therefore, freeze-drying feces prior to DNA extraction is recommended for Illumina-based microbial community analysis.

• Korean Journal of Environment and Ecology
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• v.21 no.5
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• pp.432-441
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• 2007

This study was done to classify the Quercus forests, along with their interpretations of environmental conditions, located in the lower montane zones of Mt. Bakdal, Gyemyeong, Boryeon, Worak, Cheondeung, Joryeong, Gugmang, Buyong, Duta, Malloe, Midong, Gamak, Sirubong, Sipjabong and Yongsanbong in Chungbuk Province, According to the methodology of the Z.-M. schools of phytosociology, the Quercus forests were classified into three communities and two subcommunities A. Quercus variabilis community, A-1. Quercus acutissima subcommunity, A-2. Typical subcommunity; B. Quercus mongolica community; C. Quercus dentata community. These vegetation units were arranged by human interferences and a gradient of environmental complex such as altitudes, and also were corresponded relatively well with the result of cluster analysis(UPGMA), based on the dominance of component species.

• Journal of Korean Biological Nursing Science
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• v.20 no.1
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• pp.38-46
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• 2018

Purpose: The aim of this study was to verify the effects of daily 2% chlorhexidine gluconate (CHG) bathing on the acquisition of multidrug-resistant organisms (MDRO) and healthcare-associated infection (HAI) in a medical intensive care unit (MICU). Methods: The study was a randomized controlled group posttest only design, involving 91 patients in MICU at a tertiary hospital (47 patients in the experimental group and 44 patients in the control group). The 2% CHG bathing was performed daily according to bathing protocol to the patients in the experimental group, and traditional bath was performed every three days to those in the control group. Fisher's exact test and x2 test were used to analyze the data. Results: MDRO were found in 6 patients of the experimental group and in 15 patients of the control group. The difference was statistically significant (p= .016). HAI occurred in 2 patients of the experimental group and in 7 patients of the control group. The difference was not statistically significant (p= .084). Conclusion: The results confirmed that daily bathing with CHG was effective in reducing the incidence of MDRO acquisition. Therefore, it is expected that daily bathing with CHG will be used as an effective nursing intervention to reduce the incidence of MDRO acquisition.