• Molecules and Cells
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• 제25권1호
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• pp.43-49
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• 2008

Cancer Osaka thyroid, also known as Tpl-2 (Cot) is a member of the MAP3K kinase family and plays a key role in the regulation of the immune response to pro-inflammatory stimuli such as lipopolysaccharide (LPS) and tumour necrosis $factor-{\alpha}$ ($TNF-{\alpha}$). A series of Cot constructs with an N-terminal 6xHis tag were transiently expressed in HEK293 cells: $Cot_{130-399}$ (kinase domain), $Cot_{1-388}$ (N-terminal and kinase do-mains), $Cot_{1-413}$, $Cot_{1-438}$ (containing a putative PEST sequence), $Cot_{1-457}$ (containing both PEST and degron sequences) and $Cot_{1-467}$ (full-length protein). These Cot proteins were pulled down using an anti-6xHis antibody and separated by 2D electrophoresis. The gels were silver-stained and 21 proteins were detected that did not appear, or had substantially reduced intensity, in the control sample. Three of these were identified by MS and MS/MS analysis as Hsp90, Hsp70 and Grp78. Hsp90 appeared to bind to the kinase domain of Cot and this interaction was further investigated using co-immuno-precipitation with both overexpressed Cot in HEK293 cells and endogenous Cot in Hela cells.

• Food Science and Biotechnology
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• 제16권3호
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• pp.337-342
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• 2007

Conventional methods for pathogen detection and identification are labor-intensive and take days to complete. Biosensors have shown great potential for the rapid detection of foodborne pathogens. Fiber-optic biosensors have been used to rapidly detect pathogens because they can be very sensitive and are simple to operate. However, many fiber-optic biosensors rely on manual sensor handling and the sandwich assay, which require more effort and are less sensitive. To increase the simplicity of operation and detection sensitivity, a binding inhibition assay method for detecting Listeria monocytogenes in food samples was developed using an automated, fiber-optic-based immunosensor: RAPTOR (Research International, Monroe, WA, USA). For the assay, fiber-optic biosensors were developed by the immobilization of Listeria antibodies on polystyrene fiber waveguides through a biotin-avidin reaction. Developed fiber-optic biosensors were incorporated into the RAPTOR to evaluate the detection of L. monocytogenes in frankfurter samples. The binding inhibition method combined with RAPTOR was sensitive enough to detect L. monocytogenes ($5.4{\times}10^7\;CFU/mL$) in a frankfurter sample.

• 생물정신의학
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• 제4권2호
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• pp.234-236
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• 1997

Though initial report from Japan showed positive association of schizophrenia with dinucleotide repeat polymorphism in the NT-3 gene, subsequent studies showed mixed results. Therefore we conducted a replication study with Korean schizophrenics and matched controls who share similar ethnic background with Japanese population. The frequency of allele of dinucleotide repeat at 147 base pairs in the NT-3 gene was slightly increased, however, failed to reach statistical significance(${\chi}^2$=1.884, df=1, p<0.170) between the two groups. These findings do not support an association of NT-3 gene polymorphism with schizophrenia in Korean sample.

• Toxicological Research
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• 제17권
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• pp.319-321
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• 2001

Taihu Lake is a major water source for part of Yangtze Delta, which is one of the most urbanized and economically prosperous areas in China. In last couple of decades, some parts of the lake were highly polluted due to eutrophication. This study analyzed dioxin-like potential and mutagenic potential in surface water samples from Taihu Lake. The samples were prepared by XAD-2 resin procedure. A batch of biological assays, including dioxin-like potential microassay with the rat hepatocyte cell line H411E, and Ames test was employed in the research. Results showed that jour water samples have high content of dioxin-like biological potential, the highest activity TEQ to 2,3,7,8-TCDD was 48 pg/ι in sample 1. The mutagenic effect with reading-frame shifting mechanism was confirmed in 3 of 4 samples. The effective sewage treatment facilities and reliable monitoring surveillance system are urgently needed for this area.

• 환경생물
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• 제21권4호
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• pp.358-365
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• 2003

A bacterial strain capable of producing a novel bioflocculant was isolated from a biofilm sample and identified as Bacillus megaterium G31. The highest biopolymer yield was achieved when the organism was cultivated in a medium containing acetate as the sole carbon source and ($NH_4)_2HPO_4$as the nitrogen source. In kaolin suspension, the flocculating activity was highest at 170 mg I$^{-1}$ and decreased at the higher bioflocculant concentrations. The crude bioflocculant produced by the organism was purified by ethanol precipitation and gel permeation chromatography. The FTIR spectrum of the purified bioflocculant revealed that the bioflocculant might be a heterogeneous polysaccharide composed of hexosamines and neutral sugars. The analysis of sugar components of the bioflocculant using high performance anion-exchange chromatography showed that the sugar constituents of the bioflocculant were glucosamine, fucose, galactosamine, galactose, glucose, mannose in approximate molar ratio of 4 : 1 : 6 : 3 : 8 : 19. Its flocculating activity was stimulated by various cations. The bioflocculant was thermo-stable and retained 64% of its original activity after heating at $100^{\circ}C$ for 50 min.

• 대한의용생체공학회:의공학회지
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• 제37권5호
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• pp.178-185
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• 2016

Tear is a promising biological fluid for non-invasive health monitoring. It has been studied in the past to be a possible candidate for the diagnosis of certain systemic diseases, such as breast cancer, multiple sclerosis, prostate cancer, and diabetes. However, currently existing methods for collecting and extracting tear from the human eye causes inconsistencies in the biomolecule concentrations of the tear sample due to the irritating nature of the process. In response, we designed and fabricated a microfluidic system embedded soft contact-lens for the purpose of tear sampling. The lens was then tested with artificial tear for its tear sampling capability, and found to be able to find concentration equilibrium within 50 minutes. Additionally, simulation was carried out to further optimize the design so that tear sampling rate matched the natural tear turn-over rate of 1 microliter per minute.

• 여성건강간호학회지
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• 제14권2호
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• pp.93-103
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• 2008

Purpose: This descriptive study was to explore the factors relating to Social Physique Anxiety(SPA) in the adolescent girls(N=700). These factors included the biological(level of school[age], period of menstruation, and BMI), the sociocultural(Influence of Mass Media, [IMM], Stereotype of Ideal Female Body[SIFB], type of school, perceived figure, and ideal figure) and the BW control-related factors(experience of bodyweight [BW] control, satisfaction at the result of BW control, and motive of BW control). Method: The sample was 700 girls of the middle and high schools in Jeju-do. The data was collected by the self-reported questionnaire and processed with the SPSS Win 12.0 program. Result: 26% of the variance in SPA was explained by the biological factors(level of school) and the sociocultural factors (perceived figure, ideal figure, type of school, and IMM). SPA, IMM, and SIFB were significantly correlated with each others(r=1,65-2.92, p<.05). The mean SPA scores according to BW control-related factors were significantly different. Conclusion: This results suggest that media education is necessary to reduce or eliminate the adolescent girl's SPA. In addition, SPA needs to be considered as the important concept for the future nursing researches and interventions related to BW control.

• Applied Biological Chemistry
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• 제27권4호
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• pp.252-258
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• 1984

칡 전분의 수분을 18, 21 및 24%로 조절하고, $100^{\circ}C$에서 16시간 가열 처리한 다음, 물리화학적 성질을 규명하였다. X-ray회절 양상은 수분을 24%에서 열처리한 경우 C형에서 A형으로 전환되었고, 수분 처리 수준이 높아질수록 팽화력 및 용해도는 감소하였다. 수분(24%)-열처리한 시료는 동일팽화력에서 대조구보다 높은 용해도를 보였으며, 물 결합 능력은 수분처리 수준이 높을수록 현저히 증가하였다. 칡 전분은 가열 및 냉각에 대하여 안정된 paste 점도를 보였으며, 수분-열처리에 의하여 호화온도는 증가하였고 점도는 감소하였다.

• Applied Biological Chemistry
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• 제51권4호
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• pp.251-257
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• 2008

Different types of milk products, such as kwacha golla, mawa, cheese, curd, and chocolate are popular in Bangladesh. However, the microbiological safety of these products is poorly understood. This study was performed to assess the microbiological quality of kwacha golla, a local milk product. Kwacha golla samples were collected from ten different areas of Rajshahi and Kushtia regions, and the quality of the each sample was assessed using various parameters including standard plate count, total coliform, fecal coliform, total fungi, and spore-forming bacteria, as well as food-borne microorganisms. Out of 300 samples, total coliform was detected at 56.66% (n= 300), exceeding the minimum allowable limit of 36.66%. Similarly, experiments were carried out with fungi and food-borne pathogens including Escherichia coli, Listeria monocytogenes, Salmonella sp., and Staphylococcus aureus. Results revealed 85.33, 53, and 49.33% of the samples were contaminated by fungi, E. coli, and L. monocytogenes, respectively. However, all samples showed no contaminations of Salmonella sp. and Staphylococcus sp. Therefore, this study could be helpful to the people of Bangladesh by providing information on the possibility of a major health problem caused by the consumption of kwacha golla.

• Applied Biological Chemistry
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• 제20권3호
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• pp.285-291
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• 1977

(가) 2개 이상(以上) t개의 시료(試料)를 3점기호척도시험법(點嗜好尺度試驗法)에 의해 실험한 결과를 통계처리(統計處理)하여 t개 시료 상호간(相互間)의 품질차(品質差)를 비교(比較)하는 해석법(解析法)에 대해서 검토(檢討) 하였다. (나) 그 결과 $Scheff{\acute{e}}'s$ method의 제1신법(第1新法)이 품질차(品質差)의 검출력(檢出力)에서나 얻어지는 정보량(情報量)에서 우수(優秀)함을 확인하고 t개의 시료에 대할 3점기호척도시험법(點嗜好尺度試驗法)의 해석방법(解析方法)으로써 실용성(實用性)이 있다는 결론을 얻었다. (다) 이때 correct oddity chosen에 대한 incorrec toddity chosen의 weight fraction은 chance probability에 의해 계산하여 1/2로 하였다.