• Korean Journal of Soil Science and Fertilizer
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• v.45 no.3
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• pp.421-427
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• 2012

Nepal is one of the least urbanized countries in the world where more than 80% of the total population live in rural areas. In recent years, the rate of urbanization became rampant which ultimately accelerated immense pressure on municipal services, especially on managing the ever increasing amount of wastes. Due to lack of technology, infrastructure and financial capacity management of increasing amount of solid waste has become a major challenge in municipalities of Nepal. The indiscriminate dumping of solid wastes already affected the urban environment by creating a serious occupational health and environmental hazard to the vicinity of the dumping sites. However, there is great possibility of recovering methane from the landfill sites since the typical Nepalese municipal solid waste contains more than 65% of organic wastes. Despite having enormous potential of generating electricity from hydropower, Nepal is facing acute shortage of energy. Therefore, comprehensive scientific research and development is necessary for making solid waste to environmentally friendly by converting waste to the energy.

• Korean Journal of Soil Science and Fertilizer
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• v.48 no.2
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• pp.119-124
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• 2015

Pyoverdines (PVDs) are organic compounds produced by the fluorescent Pseudomonads under iron starvation conditions. Among the isolated rhizosphere pseudomonads strains, P. putida KNUK9 showed the highest production of PVDs and its production reached to 62.81% siderophores units. DNA isolation, ligation, PCR amplification, and transformation using E. coli $DH5{\alpha}$ cells were carried out for preparing the strong pyoverdine producer strains. We detected seven genes playing the fundamental roles in the pyoverdine metabolism in Pseudomonads. According to data and analysis obtained from the study, we deduced that the strain P. putida KNUK9 contains the essential genes required for pyoverdine biosynthesis.

• Journal of Animal Environmental Science
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• v.10 no.2
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• pp.101-110
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• 2004

A solid of Thermophilic Aerobic Oxidation(TAO) System was mixed with sawdust or a rice husks. After fermentation was finished, molding machine and a dryer were used, and pellet fertilizer was produced. The fertilizing experiment was carried out as five pieces by Bed soil, TAO solid(TAO-S), TAO pellet fertilizer(TAO-PF), Chemical fertilizer(NPK) and Control(no fertilizer). Growth rate of the Chinese cabbage by each treatment was examined. Analysis of microbe and soil characteristic before and after crop experiment were carried out. When the moisture contents of TAO-PF were $18\%$ and $25\%$, the occurrence rate of microbes for the storage time was increased to $80\%$ and $100\%$ respectively. However, in the $12\%$ of water content treatment was not increased microbes. The concentration of soil bacteria in TAO-PF and TAO-S for 15 day after treatment was $1.5\times10^7\~8.0\times10^7$ CFU/ml, and the concentration of bacteria for 50 day was increased to $6.3\times10^7$ and $8.3\times10^7$ CFU/ml. However, Fungus decreased. The concentration of Actinomycetes was increased in TAO solid, Bed soil and TAO-PF treatment. The TAO-S and TAO-PF treatment were normal to compare to the NPK treatment. In this experiment the height and width of the Chinese cabbage were 22.3 cm, 16.8 cm in Bed soil and 28.8 cm, 21.3 cm in TAO solid. The leaf number of TAO-S, TAO-PF and NPK treatment were similar to 39.8, 38.3, 40.3 sheet. As the result, the TAO-PF knew that use was possible with fertilizer.

• Proceedings of the Botanical Society of Korea Conference
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• 2002.04a
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• pp.4-6
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• 2002

• Korean Journal of Soil Science and Fertilizer
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• v.47 no.6
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• pp.533-539
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• 2014

Mine wastes such as acid mine drainage (AMD) can cause the detrimental effects on surrounding environment, thereby eventually threatening human health. Main objective of this study was to evaluate the neutralizing effect of fly ash (FA) as a stabilizing material AMD. Field plot was constructed in a coal waste depot which has caused aluminium-whitening adjacent to the stream. Different mixing ratios of FA were applied on a top of the soil, and then the physicochemical properties of runoff and soil were monitored. Constructed plots were as following: control (mine waste only (W)), mine waste + 20% ($w\;w^{-1}$)of FA (WC20M), mine waste + 40% ($w\;w^{-1}$)of FA (WC40M), and WC40M dressed with a fresh soil at the top (WC40MD). Result showed that initial pH of runoff in control was 5.09 while that in WC40M (7.81) was significantly increased. For a plot treated with WC40M, the concentration of Al in runoff was decreased to $0.22mg\;L^{-1}$ compared to the W as the control ($4.85mg\;L^{-1}$). Moreover, the concentration of Fe was also decreased to less than half at the WC40M compared to the control. Application of FA can be useful for neutralizing AMD and possibly minimizing adverse effect of AMD in mining area.

• Korean Journal of Soil Science and Fertilizer
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• v.49 no.5
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• pp.608-613
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• 2016

Arbuscular mycorrhizal fungi (AMF) spore propagation and long term maintenance is still a complicated technique for farmers. The use of AMF for their ability to promote plant growth and protect plants against pathogen attack and environmental stresses demands AMF propagation for large scale application. This study aimed to propagate AMF spores by trap culture technique and assess their ability to propagate with different host plants in a continuous plant cycle. Mycorrhizal inoculation by trap culture in maize resulted in longer shoots and roots than sudangrass plants. Increase in dry weight with higher percentage also was observed for maize plants. After first and second plant cycle, maize plants had the higher percentage of mycorrhizal response in terms of colonization and arbuscules than sudangrass. Maximum in spore count also achieved in the pots of maize plants. The results show that maize plant is more suitable host plant for AMF spore propagation and trap culture technique can be used effectively to maintain the AMF culture for long time.

• Korean Journal of Soil Science and Fertilizer
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• v.49 no.1
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• pp.87-92
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• 2016

With the population growth and industrialization, the characteristics of discharged waste water and sewage have become more diverse. The removal of phosphorus (P) in the wastewater is essential for the prevention of eutrophication in the river and stream. This study was performed in order to estimate the field application of the Bacillus sp. 3434 BRRJ. Bacillus sp. 3434 BRRJ was cultured in the raw wastewater and synthetic medium at the 5 L reactor. The best optimum conditions for P removal by Bacillus sp. 3434BRRJ in the synthetic medium at the 5 L reactor were as follows: temperature, $30^{\circ}C$; P concentration, 20 mg/L; carbon sources, glucose + acetate (1:1); oxygen concentration, alternatively anaerobic and aerobic conditions. P removal efficiency under the optimum condition was 89.4%. In case of wastewater, P removal efficiency was 95.5% under controlled at $30^{\circ}C$. Through this study we confirmed that P removal by Bacillus sp. 3434BRRJ in case of wastewater was as effective as the synthetic medium. It is considered that Bacillus sp. 3434 BRRJ can be applied to the treatment of wastewater in order to biologically remove P from the wastewater on a large scale.

• Korean Journal of Soil Science and Fertilizer
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• v.50 no.1
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• pp.65-70
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• 2017

Soil was regarded as infinite resources but recently, soil is considered as invaluable resources that we need to protect and conserve. Main objective of this research was to evaluate soil value in terms of soil carbon contents. Soil was classified into forest, paddy, upland, and grass. Carbon contents in each soil was calculated based on soil chemical properties. Calculated soil carbon contents was ranged $15.31-108.86mg\;kg^{-1}$. Based on soil carbon contents, soil value was assumed adapting economic concepts. Calculated total soil value based on soil carbon contents was about 18.46 trillion won. Among others, carbon contents in forest was the highest and value was assumed 11.95 trillion won followed by paddy field (3.7 trillion won).

• Korean Journal of Soil Science and Fertilizer
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• v.50 no.1
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• pp.56-64
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• 2017

Soil quality has been regarded as an important factor for maintaining sustainability of ecosystem. Main purpose of this research was i) to select minimum factor for predicting biomass, and ii) to calculate soil quality index for biomass according to soil chemical properties. Result showed that soil pH, electrical conductivity (EC), soil organic matter (SOM), cation exchange capacity (CEC), and available phosphorus are minimum data set for calculating biomass production in soil. Selected representative soil chemical properties were evaluated for soil quality index and rated from 1 to 5 (1 is the best for biomass production). Percentage of each grade in terms of biomass production in national wide was 14.52, 35.23, 33.03, 6.47, 10.75% respectively. Although, only soil chemical properties were evaluated for calculating optimum soil quality, result of this research can be useful to understand basic protocol of soil quality assessment in national wide.

• Korean Journal of Soil Science and Fertilizer
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• v.44 no.4
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• pp.615-624
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• 2011

last few decades. In this study, the lyophilized cells of Pleurotus eryngii (mushroom) were used as an inexpensive biosorbent for Cd(II) and Pb(II) removal from aqueous solutions. The effect of various physicochemical factors on Cd(II) and Pb(II) biosorption such as pH (2.0-7.0), initial metal concentration ($0.0-300mg\;L^{-1}$), temperature, fungal biomass and contact time (0-120 min) were studied. Optimum pH for removal of Cd(II) and Pb(II) was 6.0, and the contact time was 45 min at room temperature. The nature of biosorbent and metal ion interaction was evaluated by Infrared (IR) spectroscopic technique. IR analysis of mushroom biomass revealed the presence of amino, carboxyl, hydroxyl and methyl groups, which are responsible for biosorption of Cd(II) and Pb(II). The maximum adsorption capacities of P. eryngii for Pb(II) and Cd(II) calculated using Langmuir adsorption isotherm were 82.0 and $16.13mg\;g^{-1}$, respectively. The adsorption isotherms for two biosorbed heavy metals were fitted well with Freundlich isotherm as well as Langmuir model with correlation coefficient ($r^2$>0.99). Thus, this study indicated that the P. eryngii is an efficient biosorbent for the removal of Cd(II) and Pb(II) from aqueous solutions.