• 한국초지조사료학회지
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• 제33권3호
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• pp.206-212
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• 2013

This study was conducted to investigate the effect of biological membrane transfer modifier, lysophospholipd (LPLs) on the parameters from in vitro rumen simulated fermentation. Commercially available LPLs product (Lipidol$^{TM}$) was supplemented into experimental diets which consisted of orchard grass and concentrate diet (60:40) in different levels (0.1%, 0.3% and 0.5%). Then in vitro rumen simulated fermentation was performed. Although, a declining trend of pH was found in treatments, all pH values were detected in a range relevant to normal rumen fermentation. Gas production, ammonia nitrogen and total VFA production were greatly influenced by the supplementation of LPLs. All parameters were increased along with increased levels of LPLs in diet. As a result, 0.1% of Lipidol$^{TM}$ is recommended based on the determined in vitro rumen fermentative parameters in this study.

• 생태와환경
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• 제42권4호
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• pp.487-494
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• 2009

We compared spatial and temporal variations of water chemistry between high-flow year ($HF_y$) and low-flow year ($LF_y$) in an artificial lentic ecosystem of Daechung Reservoir. The differences in the rainfall distributions explained the variation of the annual inflow and determined flow characteristics and water residence time and modified chemical and biological conditions, based on TP, suspended solids, and chlorophylla, resulting in changes of ecological functions. The intense rainfall and inflow from the watershed resulted in partial disruption of thermal structure in the metalimnion depth, ionic dilution, high TP, and high suspended solids. This condition produced a reduced chlorophyll-a in the headwaters due to low light availability and rapid flushing. In contrast, reduced inflow and low rainfall by drought resulted in strong thermal difference between the epilimnion and hypolimnion, low inorganic solids, high total dissolved solids, and low phosphorus in the ambient water. The riverine conditions dominated the hydrology in the monsoon of $HF_y$ and lacustrine conditions dominated in the $HF_y$. Overall data suggest that effective managements of the flow from the watershed may have an important role in the eutrophication processes.

• Journal of Microbiology and Biotechnology
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• 제16권9호
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• pp.1429-1433
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• 2006

Application of recombinant protein production and particularly their isotopic enrichment has stimulated development of a range of novel multidimensional heteronuclear NMR techniques. Peptides in most cases are amenable to assignment and structure determination without the need for isotopic labeling. However, there are many cases where the availability of $^{15}N$ and/or $^{13}C$ labeled peptides is useful to study the structure of peptides with more than 30 residues and the interaction between peptides and membrane. CRAMP (Cathelicidin-Related AntiMicrobial Peptide) was identified from a cDNA clone derived from mouse femoral marrow cells as a member of cathelicidin-derived antimicrobial peptides. CRAMP was successfully expressed as a GST-fused form in E. coli and purified using affinity chromatography and reverse-phase chromatography. The yield of the CRAMP was 1.5 mg/l 1. According to CD spectra, CRAMP adopted ${\alpha}$-helical conformation in membrane-mimetic environments. Isotope labeling of CRAMP is expected to make it possible to study the structure and dynamic properties of CRAMP in various membrane systems.

• 대한의용생체공학회:의공학회지
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• 제17권1호
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• pp.43-48
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• 1996

We investigated the energistics of the physiological heart model by comparing predictive indexes of the myocardial oxygen consumption (MOC), such as tension-time index (R), tension-time or force-time inteual (FTI), rate-pressure product (RPP), pressure-work index, and systolic pressure-volume area (PVA) when using the electro-hydraulic left ventricular device (LVAD). We developed the model of LVAD incorporated the closed-loop cardiovascular system with a baroreceptor which can control heart rate and time-varying elastance of left and right ventricles. On considering the benefit of the LVAD, the effects of various operation modes, especially timing of assistance, were evaluated using this coupled computer model. Overall results of the computer simulation shows that our LVAD can unload the ischemic (less contractile) heart by decreasing the MU and increasing coronary flow. Because the pump ejection at the end diastolic phase of the natural heart may increase the afterload of the left ventricle, the control scheme of our LVAD must prohibit ejecting at this time. Since the increment of coronary flow is proportional to the peak aortic pressure after ventricle contraction, the LVAD must eject immediately following the closure of the aortic valve to increase oxygen availability.

• Bulletin of the Korean Chemical Society
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• 제26권6호
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• pp.952-958
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• 2005

Angiotensin-converting enzyme (ACE) is known to be primarily responsible for hypertension. Threedimensional quantitative structure-activity relationship (3D-QSAR) models have been constructed using the comparative molecular field analysis (CoMFA) and comparative molecular similarity indices analysis (CoMSIA) for a series of 28 ACE inhibitors. The availability of ACE crystal structure (1UZF) provided the plausible biological orientation of inhibitors to ACE active site (C-domain). Alignment for CoMFA obtained by docking ligands to 1UZF protein using FlexX program showed better statistical model as compared to superposition of corresponding atoms. The statistical parameters indicate reasonable models for both CoMFA ($q^2$ = 0.530, $r^2$ = 0.998) and CoMSIA ($q^2$ = 0.518, $r^2$ = 0.990). The 3D-QSAR analyses provide valuable information for the design of ACE inhibitors with potent activity towards C-domain of ACE. The group substitutions involving the phenyl ring and carbon chain at the propionyl and sulfonyl moieties of captopril are essential for better activity against ACE.

• Animal cells and systems
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• 제16권4호
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• pp.343-349
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• 2012

A variety of fish species undergo an ontogenetic change in prey selectivity, and several potentially interacting factors, including nutrient requirement, microhabitat change, and foraging ability, may account for the occurrence of the shift. Here we examine the foraging ecology and ontogenetic diet shift of a micro-carnivorous goby, Pterogobius elapoides (serpentine goby), dominant component of fish assemblage in shallow rocky areas off the coast in Korea and Japan. Although most other gobies are primarily benthic carnivores, P. elapoides is a semipelagic fish; however, little is known about how those species change their foraging tactics with growth. In our diet analyses, the most common diet was pelagic copepods and benthic amphipods, and diet shift was observed from pelagic to benthic with growth. The ontogenetic diet shift seems to be the result of the preference for energetically more profitable prey in larger-size classes as well as the results of different prey availability due to among-habitat variation in diet. However, differential food preference does not appear to affect individual scope for searching food. Several factors such as predation pressures and interspecific resource partitioning might contribute to the changes in diet observed among size classes, which were included in our ongoing tests.

• Ocean Science Journal
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• 제40권4호
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• pp.183-190
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• 2005

We investigated the seasonal variability of tree alkaline phosphatase activity in seawater and alkaline phosphatase hydrolysable phosphorus (APHP) at 3 stations in Hiroshima Bay using alkaline phosphatase extracted from the dinoflagellates Alexandrium tamarense and Gymnodinium catenatum. The dissolved inorganic phosphorus (DIP) was lower than $1\;{\mu}M$ all samples; the lowest values were in May. The amount of APHP was high at the surface and bottom waters of all stations in May, showing DIP-depleted conditions. In August and November, the amount of APHP was much less than the amount of APHP in May, indicating that the availability of dissolved organic phosphorus (DOP) for these species was low and/or uptake during the dinoflagellate blooming might have occurred in the area. The results obtained from short-term variations of AP activity might suggest that the growth of dinoflagellates in this season may be partly supported by the AP produced by other diatoms.

• 생태와환경
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• 제49권4호
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• pp.271-278
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• 2016

Forest clear-cutting operations influence biodiversity through habitat changes and food resource availability for inhabitant species. This study examined the effects of clear-cutting on forest arthropod communities. Arthropods were collected from two different forest treatment areas (clear-cut and control) in summer and autumn. In each treatment area, arthropods were sampled from both crown and ground surfaces using sweeping and pitfall trap methods, respectively. Then, the taxonomic order of the collected arthropod specimens was easily identified. Results indicate that arthropod abundance and number of taxa present were higher at ground surface than at crown levels in both clear-cut and control areas. At crown level, more homopteran species were present in clear-cut areas than in control areas in summer. At ground surface, populations of Isopoda and Opiliones were higher in control areas than in clear-cut areas, whereas numbers of Araneae, Orthoptera, and Hymenoptera were greater in clear-cut areas. Cluster analysis and principal component analysis showed distinct differences between clear-cut and control communities at crown level in summer and at ground surface in autumn. Thus, our results indicate that clear-cutting significantly influences arthropod communities, and higher taxa are valuable for conducting rapid biological assessments of ecosystem disturbances.

• 한국식품과학회지
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• 제4권2호
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• pp.61-71
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• 1972

Se은 비타민 E의 기능과 비슷한 기능을 가지고 있으므로 필수원소로 생각되나, 허용량을 조금만 넘으면 중독증을 일으키는 유해원소이다. 이러한 Se분석은 종래방법은 시료분해중 Se의 증발로 인한 손실과 시약의 오염으로 오는 오차로 분석이 힘들었다. 그러나 Se분석을 $^{77m}Se$(반감기 18초)의 핵종을 이용하여 비파괴 분석으로 간편하고 고감도로 미량을 신속하게 방사화 분석을 이용하여 분석할 수가 있다. 본 실험은 한국산 곡류, 야채, 과일, 건초, 육류, 해산물 등을 분석한 결과를 외국산 것과 비교한 결과를 발표한다.

• 대한원격탐사학회지
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• 제35권1호
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• pp.93-115
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• 2019

The availability of high-resolution laser scanning data and advanced machine learning algorithms has enabled an accurate potential rockfall source identification. However, the presence of other mass movements, such as landslides within the same region of interest, poses additional challenges to this task. Thus, this research presents a method based on an integration of Gaussian mixture model (GMM) and ensemble artificial neural network (bagging ANN [BANN]) for automatic detection of potential rockfall sources at Kinta Valley area, Malaysia. The GMM was utilised to determine slope angle thresholds of various geomorphological units. Different algorithms(ANN, support vector machine [SVM] and k nearest neighbour [kNN]) were individually tested with various ensemble models (bagging, voting and boosting). Grid search method was adopted to optimise the hyperparameters of the investigated base models. The proposed model achieves excellent results with success and prediction accuracies at 95% and 94%, respectively. In addition, this technique has achieved excellent accuracies (ROC = 95%) over other methods used. Moreover, the proposed model has achieved the optimal prediction accuracies (92%) on the basis of testing data, thereby indicating that the model can be generalised and replicated in different regions, and the proposed method can be applied to various landslide studies.