• Title/Summary/Keyword: biological application

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A New Material for Rapid and Easy Method of Plant Surface Imprinting

  • Bhat, R.B.;Etejere, E.O.
    • Journal of Plant Biology
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    • v.28 no.4
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    • pp.329-332
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    • 1985

  • A simple new device for obtaining very clear epidermal imprints for light microscopic studies is discussed. This new device is developed from“Britfix”(polystyrene cement) which is non-toxic to the plant organs. It involves direct application of the material on the desired surface of the plant organ to obtain thin, transparent replica. From the present investigation“Britfix”is found to be useful for the study of epidermal anatomy, morphology and physiology. Epidermal imprints can be mounted on the microscope slide without a mounting medium. Permanent slide of these imprints can be kept for any desired period without any deterioration of the replica.

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Functional Study of Lysine Decarboxylases from Klebsiella pneumoniae in Escherichia coli and Application of Whole Cell Bioconversion for Cadaverine Production

  • Kim, Jung-Ho;Kim, Hyun Joong;Kim, Yong Hyun;Jeon, Jong Min;Song, Hun Suk;Kim, Junyoung;No, So-Young;Shin, Ji-Hyun;Choi, Kwon-Young;Park, Kyung Moon;Yang, Yung-Hun
    • Journal of Microbiology and Biotechnology
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    • v.26 no.9
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    • pp.1586-1592
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    • 2016

  • Klebsiella pneumoniae is a gram-negative, non-motile, rod-shaped, and encapsulated bacterium in the normal flora of the intestines, mouth, skin, and food, and has decarboxylation activity, which results in generation of diamines (cadaverine, agmatine, and putrescine). However, there is no specific information on the exact mechanism of decarboxylation in K. pnuemoniae. Specifically lysine decarboxylases that generate cadaverine with a wide range of applications has not been shown. Therefore, we performed a functional study of lysine decarboxylases. Enzymatic characteristics such as optimal pH, temperature, and substrates were examined by overexpressing and purifying CadA and LdcC. CadA and LdcC from K. pneumoniae had a preference for L-lysine, and an optimal reaction temperature of 37℃ and an optimal pH of 7. Although the activity of purified CadA from K. pneumoniae was lower than that of CadA from E. coli, the activity of K. pneumoniae CadA in whole cell bioconversion was comparable to that of E. coli CadA, resulting in 90% lysine conversion to cadaverine with pyridoxal 5'-phosphate L-lysine.

  • https://doi.org/10.4014/jmb.1602.02030 인용 PDF KSCI KPUBS HTML

Expression of Antioxidant Isoenzyme Genes in Rice under Salt Stress and Effects of Jasmonic Acid and ${\gamma}$-Radiation

  • Kim, Jin-Hong;Chung, Byung-Yeoup;Baek, Myung-Hwa;Wi, Seung-Gon;Yang, Dae-Hwa;Lee, Myung-Chul;Kim, Jae-Sung
    • Journal of Applied Biological Chemistry
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    • v.48 no.1
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    • pp.1-6
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    • 2005

  • Analysis of chlorophyll (Chl) fluorescence implicated treatment of 40 mM NaCl decreased maximal photochemical efficiency of photosystem II (PSII) (Fv/Fm), actual quantum yield of PSII (${\Phi}_{PSII}$), and photochemical quenching (qP) in rice, but increased non-photochemical quenching (NPQ). Decreases in Fv/Fm, ${\Phi}_{PSII}$, and qP were significantly alleviated by $30\;{\mu}M$ jasmonic acid (JA), while NPQ increase was enhanced. Transcription levels of antioxidant isoenzyme genes were differentially modulated by NaCl treatment. Expression of cCuZn-SOD2 gene increased, while those of cAPXb, CATb, and CATc genes decreased. JA prevented salt-induced decrease of pCuZn-SOD gene expression, but caused greater decrease in mRNA levels of cAPXa and Chl_tAPX genes. Investigation of vacuolar $Na^+/H^+$ exchanger (NHX2) and 1-pyrroline-5-carboxylate synthetase (P5CS) gene expressions revealed transcription level of NHX2 gene was increased by JA, regardless of NaCl presence, while that of P5CS gene slightly increased only in co-presence of JA and NaCl. Unlike JA, ${\gamma}$-radiation rarely affected expressions of antioxidant isoenzyme, NHX2, and P5CS genes, except for increase in mRNA level of Chl_tAPX and decrease in that of pCuZn-SOD. These results demonstrate enhanced salt-tolerance in JA-treated rice seedlings may be partly due to high transcription levels of pCuZn-SOD, NHX2, and P5CS genes under salt stress.

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