• 한국환경과학회지
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• 제2권2호
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• pp.95-102
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• 1993

In order to find the most fitted biodegradation model, biodegradation models to the initial 4-chlorophenol concentrations were investigated and had been fitted by the linear regression. The degrading bacterium, EL-091S, was selected among phenol-degraders. The strain was identified with Pseudomows sp. from the result of taxonomical studies. The optimal condition for the biodegradation was as fellows: secondary carbon source, concentration of ammonium nitrate, temperature and pH were 200mg/l fructose, 600 mg/l, $30^{\circ}C$ and 7.0 respectively. The highest degradation rate of the 4-chlorophenol was about 58% for 24 hours incubation on the optimal condition. Biodegradation kinetics model of 5 mg/l 4-Chlorophenol, 10 mg/l 4-chlorophenol and 50 mg/l 4-chlorophenol were fitted the zero order kinetics model, respectively. Key Words : 4-chlorophenol, Pseudomonas sp., zero order kinetics model.

• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2002년도 추계학술발표회
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• pp.92-95
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• 2002

Bioavailability study was conducted to elucidate the relationship between sorption/desorption and biodegradation of sorbed phenanthrene in seven different soils. Mineralization kinetics was determined for phenanthrene-sorbed soil slurries inoculated with Pseudomonas putida (ATCC strain 17484). Two biodegradation models were used to fit mineralization kinetics; (i) a first-order degradation model and (ii) a coupled degradation-desorption model. The biodegradation rates were in order of vermicompost >Bion peat > 50% organoclay > Pahokee > blank (no soil, medium only) > montmorillonite > Ohio shale. The mineralization rate constants increased as desorption-resistance of phenanthrene increased. Among the tested sorbents, active biodegradation of phenanthrene was observed in vermicompost and Bion peat. Biodegradation in these two sorbents exhibited little lag time and a high maximum mineralized capacity. The role of sorption/desorption in bioavailability of phenanthrene sorbed in soils was discussed.

• Environmental Engineering Research
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• 제24권2호
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• pp.309-317
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• 2019

This study evaluated the kinetics of acrylamide (AM) biodegradation by mixed culture bacteria and Enterobacter aerogenes (E. aerogenes) in sequencing batch reactor (SBR) systems with AQUASIM and linear regression. The zero-order, first-order, and Monod kinetic models were used to evaluate the kinetic parameters of both autotrophic and heterotrophic nitrifications and both AM and chemical oxygen demand (COD) removals at different AM concentrations of 100, 200, 300, and 400 mg AM/L. The results revealed that both autotrophic and heterotrophic nitrifications and both AM and COD removals followed the Monod kinetics. High AM loadings resulted in the transformation of Monod kinetics to the first-order reaction for AM and COD removals as the results of the compositions of mixed substrates and the inhibition of the free ammonia nitrogen (FAN). The kinetic parameters indicated that E. aerogenes degraded AM and COD at higher rates than mixed culture bacteria. The FAN from the AM biodegradation increased both heterotrophic and autotrophic nitrification rates at the AM concentrations of 100-300 mg AM/L. At higher AM concentrations, the FAN accumulated in the SBR system inhibited the autotrophic nitrification of mixed culture bacteria. The accumulation of intracellular polyphosphate caused the heterotrophic nitrification of E. aerogenes to follow the first-order approximation.

• 한국환경과학회지
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• 제6권2호
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• pp.153-163
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• 1997

In order to fad the most fitted biodegradation model, biodegradation kinetics model to the initial phenol and p-cresot concentrations were investigated and had been fitted by the linear regression. Bacteria capable of degrading p-cresol were isolated from soil by enrichment culture technique. Among them, strain Ml capable of degradillg p.rcresol has also degraded phenal and was identified as the genus Micrococcus from the results from of taxonomical studies. The optimal tonditlons for the biodegradation of phenal and p-cresol by Micrococcus sp. Ml were $NH_4NO_3$ 0.05%, pH 7.0, 3$0^{\circ}C$, respectively, and medium volume 100m1/250m1 shaking flask. iwicrococcus sp. Ml was able to grow on phenal concentration up to 14mM and p-cresol concelltration up to 0.8mM. With increasing substrate concentraction, the lag period increased, but the maximum specific growth rates decreased. The yield coefficient decreased with increasing substrate concentation. The biodegradation kinetics of phenol and p-cresol were best described by Monod with growth model for every experimented concentration. In cultivation of mixed substrate, p-cresol was degraded first and phenol was second. This result implies that p-cresol and phenol was not degraded simultaneously.

• 한국환경과학회지
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• 제2권4호
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• pp.271-278
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• 1993

Nonylphenol ethoxylates-30을 분해할 수 있는 Pseudomonas sp.를 분리, 동정하였다. Nonylphenol ethoxylates-30 의 최적분해조건은 탄소원으로 nonylphenol ethoxylates-30 1.0 g/ι, 질소원으로 ammonium nitrate 0.02 g/ι, pH 7.5, 30였다. 최적분해 조건에서 nonylphenol ethoxylates-30은 배양 30시간 후 89%가 분해되었다. 최적분해조건에서 nonylphenol ethoxylates-30의 초기농도가 각각 100 ppm, 500 ppm, 1000 ppm, 5000 ppm일 때 시간에 따른 분해율을 수식으로 설명할 수 있는 가장 적합한 분해 kinetics는 각각 first order model, Monod no growth model, Monod with growth model로 나타났다.

• 한국환경과학회지
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• 제6권5호
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• pp.481-488
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• 1997

Microorganisms capable of degrading trlchloroethylene(TCEI using phenol as a induction substrate were isolated from industrial effluents and soil. The strain MS-64K which had the highest blodegradablllty was identified as the genus Micrococcus. The optimal conditions of medium for the growth and blodegadatlon of trlchloroethylene were observed as follows; the initial pH 7.0, trlchloroethylene 1, 000ppm as the carbon source, 0.2% ${(NH_4)}_2SO_4$, as the nitrogen source. respectively. Lag period and degradation time on optimal medium were shorter than those on Isolation medium. Growth on the optimal medium was Increased. Addition of 0.1% Triton X-100 Increased the growth rate of Micrococcus sp. MS-64K, but degradation was equal to optimal medium. Trlchloroethylene degradation by Micrococcus sp. MS-64K was shown to fit logarithmic model when the compound was added at initial concentration of 1, 000ppm.

• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2001년도 추계학술발표회
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• pp.235-238
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• 2001

Monod kinetics에 관련된 주요 생분해 파라미터를 도출하기 위하여 microcosm 규모의 배치실험에서 BTEX 화합물에 대해 분해능이 우수한 Pseudomonas aeruginosa을 이용해 다양한 농도의 벤젠에 대한 분해기작을 고찰하였다. 벤젠의 생분해율(D)과 Maximumspecific growth rate ($\mu$$_{max}$)는 기질의 농도가 증가할수록 높아지다가 최고점에 도달 후에 점차적으로 감소하였으며 이것은 어느 한계점 이상의 벤젠 농도가 미생물의 생분해에 방해 요소로 작용한다는 것을 나타낸다. 그러나 미생물에 의한 벤젠 분해의 상관관계를 나타내는 yield coefficient(Y)는 벤젠의 초기 농도가 낮을수록 높은 값을 나타내었다. Microbial decay constant( b)와 half-saturation constant(K$_{c}$)는 각각 0.21~0.48day$^{-1}$와 218mg/$\ell$로서 문헌값 보다 높은 수치를 나타내었다. 실험으로부터 결정된 생분해 파라미터들은 초기 벤젠 농도에 따라 큰 차이를 보이므로 생분해 모델링에 사용할 파라미터는 기질농도에 따라 적절하게 선택되어야 한다고 사료된다.

• Journal of Microbiology and Biotechnology
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• 제15권5호
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• pp.946-951
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• 2005

Biodegradation of the endocrine-disrupting chemical di-n-butyl phthalate (DBP) was investigated using a bacterium, Pseudomonas fluorescens B-1, isolated from mangrove sediment. The effects of temperature, pH, salinity, and oxygen availability on DBP degradation were studied. Degradation of DBP was monitored by solid-phase extraction using reversed-phase HPLC and UV detection. The major metabolites of DBP degradation were identified as mono-n-butyl phthalate and phthalic acid by gas chromatography-mass spectrometry (GC-MS) and a pathway of degradation was proposed. Degradation by P. fluorescens B-1 conformed to first-order kinetics. Degradation of DBP was also tested in seawater by inoculating P. fluorescens B-1, and complete degradation of an initial concentration of $100{\mu}g/l$ was achieved in 144 h. These results suggest that DBP is readily degraded by bacteria in natural environments.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 춘계학술발표대회
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• pp.418-421
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• 2000

A model was developed to describe the microbial decontamination of diesel contaminated soil in a soil column. The biodegradation rate of diesel in nature depends on temperature and the pH of soil, availability of nutrients, oxygen and water. The soil moisture content is one of the essential factors because it characterizes the availability not only of water to microorganisms but also of oxygen and nutrient dissolved in soil. In this work, the rate of biodegradation was modeled by coupling Michaelis-Menten kinetics for the aqueous-phase solute with adsoption-desoption equation for diesel sorption and desorption from soil.

• 한국미생물·생명공학회지
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• 제26권5호
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• pp.465-469
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• 1998

본 연구의 목적은 토양매립조건에서 생분해성 고분자의 생분해도를 정량적이며 신속하게 측정하는 방법을 개발하는 것으로서, 실험대상 물질은 생분해가 잘되는 것으로 알려져 있는 셀로판 필름을 사용하였다. 분해정도를 측정하기 위하여 흙속에서 채취한 셀로판 필름을 HCl로 가수분해 시킨 후 생성된 glucose를 측정하는 방법을 사용하였다. 토양매립시 생분해도는 겨울에는 4개월간 고작해야 41.2%정도의 분해를 보였으나, 여름에는 2개월동안 76.5%정도의 분해도를 나타낸 결과로 보아 계절의 영향을 많이 받는 것으로 나타났다. 실험실적 토양매립조건의 실험을 위해 토양을 채취하여 플라스크에 넣고, 여기에 셀로판 필름을 묻은 후 항온배양기내에서 일정한 조건(3$0^{\circ}C$, 습도 50~55%)으로 실험하였다. 이때에는 40일 경과 후 94%정도의 분해도를 나타내었다. 분해속도는 1차 반응 속도식을 따르고 반응속도 상수는 067(1/day)이었다. 생분해도의 가속화를 위해 토양내 미생물을 필름 표면에 접종하고 플라스크 내의 흙속에 매립한 후 항온배양기내에서 생분해도를 측정한 결과, 초반 12일경까지는 미생물 접종의 효과가 뚜렷이 나타났으나 말기로 갈수록 그 효과는 없어졌다. 가속화를 위한 또 다른 방법으로 토양내 영양성분인 N, P, S 등을 추가로 공급한 결과, 초반부터 말기까지 지속적인 가속화 효과를 보임과 동시에 반응속도 상수도 0.096(1/day)에서 0.21(1/day)로 118%정도의 높은 증가를 보였다. 결과적으로, 실외에서의 토양매립조건에서는 생분해도의 반응속도 상수가 겨울기간의 0.0042(1/day), 여름기간의 0.024(1/day)로 나타났고 같은 실험실적 토양매립조건에서라도 토양을 채취한 시기에 따라 봄에 채취한 토양에서는 0.065(1/day)로 여름에 채취한 토양에서는 0.096(1/day)로 반응 속도 상수가 다르게 나타났다. 각각의 매립조건들 중에서 N, P, S 모두를 첨가한 실험실적 토양매립조건에서의 생분해도가 가장 뛰어난 것으로 나타났다.